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The map label for this gene is ilvB [H]

Identifier: 159041735

GI number: 159041735

Start: 1227557

End: 1229308

Strand: Reverse

Name: ilvB [H]

Synonym: Cmaq_1170

Alternate gene names: 159041735

Gene position: 1229308-1227557 (Counterclockwise)

Preceding gene: 159041743

Following gene: 159041734

Centisome position: 59.17

GC content: 47.37

Gene sequence:

>1752_bases
ATGAATCAAAATCAAGTGAATCAAATAGGGGTGGTTACGGTGGTTAGGGCTGTTGATGCATTGGCTGATGAAATAGTTAA
AATGAATATTAGGGAAGTCTTCGGCATACCGGGAGGCCAAATAATGCCACTATTCGATGCCTTCTACGGTAAGGAAGCTA
AAATATTCCTCTTTAGGCATGAACAAGGGGCAATTCACGCCGCTGATGCCTACGGTAGAGTCACTAAAAGACCTGGGATT
GTAATGGTTACCTCAGGTCCAGGGGCAACTAACCTGGCCACTGGCTTGGCTAACGCAATGATGGATTCATCACCCCTAGT
GGCCTTAACGGGTCAAGTACCAACCTCAGTGTTCGGTAGGGATGCATTCCAGGAGACCGACATAGTTGGTTTATCAATGC
CCATTGTTAAACACACCTTCATGGTTAAGAAACCTGAGGACCTGGTGCCAGCCTTTAAGGCAGCCTACAGGATAGCCACT
GATGGTAGACCTGGCCCAGTCTTAGTTGATATACCTAGGGATGCCCAATTATCAGAGGTTAAGGGGGATGGCTCAGGAAG
CATTAACGTTAACTACAAGGGTGTCCCTGAACCAGACATAGGCTTAGTGGCCTATGCCGTTAAACTACTAATGGAGGCTA
AGAGACCGGTTATGCTTGTTGGCGGTGGCGTCTGCTGGAGTAACGCCACTGAGGAGGCTTTAGCCATTGCTGAAACCCTA
TGGATGCCCATAGTGACCACCCTACCTGGTAAGAATTGCGTGCCGAATAATCACCCATTATTCATGGGTCCCGCTGGGAT
GCATGGTAGGGTTGAGGCTGATGCAGCCATAATTAACTCAGACCTCGTCCTGGCTGTTGGGACTAGGTTCAGCGATAGGA
CTGTGGGTGATTTTAAGGAGTTCCAGAGGGGTAGGAAGATTATTCACATTGATATTGATAAGAGTGAGATCGGTAAGAAC
GTTAAACCAAGCGTGGGCATAATAGGTGACGCTAAGGTGGCTTTAAGAATGATGCTTGAGTTAATTCCCAAGGCCGCTGT
TAAGAATGAGGCCTTCGTTAATTGGCTTAAGTCAATTAAGGAGGCCTACGAGAAGTTCAGGGAGCAGGATAACATGCCTG
GGTTCGCGCCGTGGAAGGTACTTAAGGTGATTAGGGAGGTTACACCACCTCACGCCATAACCGCAACAAGCGTAGGCAGT
CACCAAATGTGGAGTGAACTGCATTGGGATGTATACGTCCCTGGAACATTCATAACCAGTGCAGGCCTAGGAACCATGGG
TTTCGGTTTACCTGCAGCCCTAGGTGCTAAGGTGGCTAAGCCCAATGTACCTGTTATTGATATTGATGGTGATGGTTCAT
TCCAAATGACCATGCAGAACCTAGCCCTAGTTAGGGAGTATAACCTACCCATAGTAGTGGTTATCTTCGATAATTCAACG
CTTATGCTTGTTAGGCATTGGCAAATGCTACTGTACAGTAGGAGGATAATTGGGGTTGATTTCCAGGCTAACCCAGACTT
CATGAAGATAGCTGAGGCCTACGGGATAGATGGTGTTAGGCCGAGTAACTACGATGAGTTAAGGAACAGTGTGGCTAGGG
CTATTAGGAATAATGAACCACTGATTGTTGACGTTACCATAGATAGGGAAAGGGACCTGGTGCTGCCTTGGGTTCAACCT
GGTAAGTGGCTTACTGAGGTAACCCTACCTGATGGGTTTAAGGTTAACTTAAGGTATGGTGATGGGGAATGA

Upstream 100 bases:

>100_bases
ACAGTAGTTTAATGAGGTGTTTTATTCTTTACGTACGATAGGGAGGGGCCTTGGAGGGCCCAATATGAAAATATGTTAAG
TGAAGAAAGAAATCAAATGA

Downstream 100 bases:

>100_bases
ATAACTATAAGATAACCATAACCCTATTGAATAATGATGGAAGCGTGGATCCACTGGTCAGGGCGCTTAACGTGATTAGG
AGGGGTAAGGTGAACGTTAA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MNQNQVNQIGVVTVVRAVDALADEIVKMNIREVFGIPGGQIMPLFDAFYGKEAKIFLFRHEQGAIHAADAYGRVTKRPGI
VMVTSGPGATNLATGLANAMMDSSPLVALTGQVPTSVFGRDAFQETDIVGLSMPIVKHTFMVKKPEDLVPAFKAAYRIAT
DGRPGPVLVDIPRDAQLSEVKGDGSGSINVNYKGVPEPDIGLVAYAVKLLMEAKRPVMLVGGGVCWSNATEEALAIAETL
WMPIVTTLPGKNCVPNNHPLFMGPAGMHGRVEADAAIINSDLVLAVGTRFSDRTVGDFKEFQRGRKIIHIDIDKSEIGKN
VKPSVGIIGDAKVALRMMLELIPKAAVKNEAFVNWLKSIKEAYEKFREQDNMPGFAPWKVLKVIREVTPPHAITATSVGS
HQMWSELHWDVYVPGTFITSAGLGTMGFGLPAALGAKVAKPNVPVIDIDGDGSFQMTMQNLALVREYNLPIVVVIFDNST
LMLVRHWQMLLYSRRIIGVDFQANPDFMKIAEAYGIDGVRPSNYDELRNSVARAIRNNEPLIVDVTIDRERDLVLPWVQP
GKWLTEVTLPDGFKVNLRYGDGE

Sequences:

>Translated_583_residues
MNQNQVNQIGVVTVVRAVDALADEIVKMNIREVFGIPGGQIMPLFDAFYGKEAKIFLFRHEQGAIHAADAYGRVTKRPGI
VMVTSGPGATNLATGLANAMMDSSPLVALTGQVPTSVFGRDAFQETDIVGLSMPIVKHTFMVKKPEDLVPAFKAAYRIAT
DGRPGPVLVDIPRDAQLSEVKGDGSGSINVNYKGVPEPDIGLVAYAVKLLMEAKRPVMLVGGGVCWSNATEEALAIAETL
WMPIVTTLPGKNCVPNNHPLFMGPAGMHGRVEADAAIINSDLVLAVGTRFSDRTVGDFKEFQRGRKIIHIDIDKSEIGKN
VKPSVGIIGDAKVALRMMLELIPKAAVKNEAFVNWLKSIKEAYEKFREQDNMPGFAPWKVLKVIREVTPPHAITATSVGS
HQMWSELHWDVYVPGTFITSAGLGTMGFGLPAALGAKVAKPNVPVIDIDGDGSFQMTMQNLALVREYNLPIVVVIFDNST
LMLVRHWQMLLYSRRIIGVDFQANPDFMKIAEAYGIDGVRPSNYDELRNSVARAIRNNEPLIVDVTIDRERDLVLPWVQP
GKWLTEVTLPDGFKVNLRYGDGE
>Mature_583_residues
MNQNQVNQIGVVTVVRAVDALADEIVKMNIREVFGIPGGQIMPLFDAFYGKEAKIFLFRHEQGAIHAADAYGRVTKRPGI
VMVTSGPGATNLATGLANAMMDSSPLVALTGQVPTSVFGRDAFQETDIVGLSMPIVKHTFMVKKPEDLVPAFKAAYRIAT
DGRPGPVLVDIPRDAQLSEVKGDGSGSINVNYKGVPEPDIGLVAYAVKLLMEAKRPVMLVGGGVCWSNATEEALAIAETL
WMPIVTTLPGKNCVPNNHPLFMGPAGMHGRVEADAAIINSDLVLAVGTRFSDRTVGDFKEFQRGRKIIHIDIDKSEIGKN
VKPSVGIIGDAKVALRMMLELIPKAAVKNEAFVNWLKSIKEAYEKFREQDNMPGFAPWKVLKVIREVTPPHAITATSVGS
HQMWSELHWDVYVPGTFITSAGLGTMGFGLPAALGAKVAKPNVPVIDIDGDGSFQMTMQNLALVREYNLPIVVVIFDNST
LMLVRHWQMLLYSRRIIGVDFQANPDFMKIAEAYGIDGVRPSNYDELRNSVARAIRNNEPLIVDVTIDRERDLVLPWVQP
GKWLTEVTLPDGFKVNLRYGDGE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=25.2688172043011, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=25.8620689655172, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI87081685, Length=538, Percent_Identity=39.4052044609665, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=35.8834244080146, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=29.802513464991, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=25.8064516129032, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=25.3187613843352, Blast_Score=158, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=555, Percent_Identity=26.6666666666667, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=27.7486910994764, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=573, Percent_Identity=27.7486910994764, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=38.6120996441281, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6320816, Length=480, Percent_Identity=23.75, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=508, Percent_Identity=23.8188976377953, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=505, Percent_Identity=23.3663366336634, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=591, Percent_Identity=26.9035532994924, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63736; Mature: 63736

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNQVNQIGVVTVVRAVDALADEIVKMNIREVFGIPGGQIMPLFDAFYGKEAKIFLFRH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEE
EQGAIHAADAYGRVTKRPGIVMVTSGPGATNLATGLANAMMDSSPLVALTGQVPTSVFGR
CCCCEEECHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHCC
DAFQETDIVGLSMPIVKHTFMVKKPEDLVPAFKAAYRIATDGRPGPVLVDIPRDAQLSEV
CCCCCCCEEEEECHHHHHEEEECCCHHHHHHHHHHEEEECCCCCCCEEEECCCCCCHHHC
KGDGSGSINVNYKGVPEPDIGLVAYAVKLLMEAKRPVMLVGGGVCWSNATEEALAIAETL
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEECCCCHHHHHHHHHHH
WMPIVTTLPGKNCVPNNHPLFMGPAGMHGRVEADAAIINSDLVLAVGTRFSDRTVGDFKE
HHHHHEECCCCCCCCCCCCEEECCCCCCCCEECCEEEECCCEEEEECCCCCCCCHHHHHH
FQRGRKIIHIDIDKSEIGKNVKPSVGIIGDAKVALRMMLELIPKAAVKNEAFVNWLKSIK
HHCCCEEEEEECCHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
EAYEKFREQDNMPGFAPWKVLKVIREVTPPHAITATSVGSHQMWSELHWDVYVPGTFITS
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCEEEECCCEEEC
AGLGTMGFGLPAALGAKVAKPNVPVIDIDGDGSFQMTMQNLALVREYNLPIVVVIFDNST
CCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCEEEHHHHHHHHEECCCCEEEEEECCCE
LMLVRHWQMLLYSRRIIGVDFQANPDFMKIAEAYGIDGVRPSNYDELRNSVARAIRNNEP
EEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
LIVDVTIDRERDLVLPWVQPGKWLTEVTLPDGFKVNLRYGDGE
EEEEEEECCCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCC
>Mature Secondary Structure
MNQNQVNQIGVVTVVRAVDALADEIVKMNIREVFGIPGGQIMPLFDAFYGKEAKIFLFRH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEEE
EQGAIHAADAYGRVTKRPGIVMVTSGPGATNLATGLANAMMDSSPLVALTGQVPTSVFGR
CCCCEEECHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHCC
DAFQETDIVGLSMPIVKHTFMVKKPEDLVPAFKAAYRIATDGRPGPVLVDIPRDAQLSEV
CCCCCCCEEEEECHHHHHEEEECCCHHHHHHHHHHEEEECCCCCCCEEEECCCCCCHHHC
KGDGSGSINVNYKGVPEPDIGLVAYAVKLLMEAKRPVMLVGGGVCWSNATEEALAIAETL
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEECCCCHHHHHHHHHHH
WMPIVTTLPGKNCVPNNHPLFMGPAGMHGRVEADAAIINSDLVLAVGTRFSDRTVGDFKE
HHHHHEECCCCCCCCCCCCEEECCCCCCCCEECCEEEECCCEEEEECCCCCCCCHHHHHH
FQRGRKIIHIDIDKSEIGKNVKPSVGIIGDAKVALRMMLELIPKAAVKNEAFVNWLKSIK
HHCCCEEEEEECCHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
EAYEKFREQDNMPGFAPWKVLKVIREVTPPHAITATSVGSHQMWSELHWDVYVPGTFITS
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCEEEECCCEEEC
AGLGTMGFGLPAALGAKVAKPNVPVIDIDGDGSFQMTMQNLALVREYNLPIVVVIFDNST
CCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCEEEHHHHHHHHEECCCCEEEEEECCCE
LMLVRHWQMLLYSRRIIGVDFQANPDFMKIAEAYGIDGVRPSNYDELRNSVARAIRNNEP
EEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
LIVDVTIDRERDLVLPWVQPGKWLTEVTLPDGFKVNLRYGDGE
EEEEEEECCCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]