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The map label for this gene is nadE [H]

Identifier: 159041674

GI number: 159041674

Start: 1167198

End: 1168055

Strand: Reverse

Name: nadE [H]

Synonym: Cmaq_1108

Alternate gene names: 159041674

Gene position: 1168055-1167198 (Counterclockwise)

Preceding gene: 159041676

Following gene: 159041672

Centisome position: 56.22

GC content: 45.57

Gene sequence:

>858_bases
ATGCAGCCTTGGATTACCCTTGATAGGATACTGAACGTTAATTACGATTACATTGAAAGAAGGATAGTGGACTTCATAAG
AACATACGTAAACAGCGTGGGCGCTAAGGGTGCTGTTATAGGGCTTAGTGGTGGTATTGACTCAAGTGTAACCGCATCAC
TGCTGGTTAGGGCTTTAGGTAAGGATAAGGTCCTAGCCTTAATAATGCCATACAAGACAACGCCGCCTGAGGATGTTAAG
GACGCTATTCAACTAGCCCAGATGCTGGGTGTAAAGTATGATGTTGTTAACATAGACCCCATTAGGGCATCATTCTCAAG
CACAATACCTGCCTTCAAGGAAAGTGAAATTGTTGCTAACGGCAACATACTGGCTAGAATAAGGATGACGATACTGTACT
ACTACGCCAACCTAAACAACATGATTGTCGCCGGTACCGGGGATAAGAGTGAGTTATTGATAGGCTACTTCACTAAGTAT
GGTGATGGCGGTGTTGATATATTGCCAATAGGGGATGTGTATAAGAGTCAAGTTAGAATGCTTGGGAGGCGTTTAGGCTT
ACCTGACTCAATAGTCACGAAACCCTCAAGCCCACGCCTATGGGAAGGTCAGACCGCTGAGGGTGAATTGGGTGTTAGTT
ACGCTGATATTGATGTTATCCTCCATGCGTTAGTTGACTTAAGGATGAATCCACAGAAGGCTCAGGAGGCCACTGGGAAG
CCTCTTAACCTTATTGAACAGGTATGGAGGAGAGTGGTTACAACTGAGCATAAGCGTAGAACCCCAGTGGTGCCTAGGAT
TGGGTTAAGGACTATTGGGCTTGACTGGCGTATGCCGGTTCACTACAATGGCTACTAA

Upstream 100 bases:

>100_bases
AATACACCTAATAATACTTAGGTTTAAGCACAACGTATGACTATTGATTCCGTTAACCTTAAGACGAAAGCGTTAATTAG
GCTAGGTGGCGTGACCTATT

Downstream 100 bases:

>100_bases
GGTAAGGCTTCTAATACACCTCATCAATATTTAGGCGCAGTGATTCATAACTATGAACCTAATTTTATAAATACATGCCT
TACCTTAGTTTTAACACTAT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQPWITLDRILNVNYDYIERRIVDFIRTYVNSVGAKGAVIGLSGGIDSSVTASLLVRALGKDKVLALIMPYKTTPPEDVK
DAIQLAQMLGVKYDVVNIDPIRASFSSTIPAFKESEIVANGNILARIRMTILYYYANLNNMIVAGTGDKSELLIGYFTKY
GDGGVDILPIGDVYKSQVRMLGRRLGLPDSIVTKPSSPRLWEGQTAEGELGVSYADIDVILHALVDLRMNPQKAQEATGK
PLNLIEQVWRRVVTTEHKRRTPVVPRIGLRTIGLDWRMPVHYNGY

Sequences:

>Translated_285_residues
MQPWITLDRILNVNYDYIERRIVDFIRTYVNSVGAKGAVIGLSGGIDSSVTASLLVRALGKDKVLALIMPYKTTPPEDVK
DAIQLAQMLGVKYDVVNIDPIRASFSSTIPAFKESEIVANGNILARIRMTILYYYANLNNMIVAGTGDKSELLIGYFTKY
GDGGVDILPIGDVYKSQVRMLGRRLGLPDSIVTKPSSPRLWEGQTAEGELGVSYADIDVILHALVDLRMNPQKAQEATGK
PLNLIEQVWRRVVTTEHKRRTPVVPRIGLRTIGLDWRMPVHYNGY
>Mature_285_residues
MQPWITLDRILNVNYDYIERRIVDFIRTYVNSVGAKGAVIGLSGGIDSSVTASLLVRALGKDKVLALIMPYKTTPPEDVK
DAIQLAQMLGVKYDVVNIDPIRASFSSTIPAFKESEIVANGNILARIRMTILYYYANLNNMIVAGTGDKSELLIGYFTKY
GDGGVDILPIGDVYKSQVRMLGRRLGLPDSIVTKPSSPRLWEGQTAEGELGVSYADIDVILHALVDLRMNPQKAQEATGK
PLNLIEQVWRRVVTTEHKRRTPVVPRIGLRTIGLDWRMPVHYNGY

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=264, Percent_Identity=28.4090909090909, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1788036, Length=275, Percent_Identity=30.1818181818182, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 31718; Mature: 31718

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPWITLDRILNVNYDYIERRIVDFIRTYVNSVGAKGAVIGLSGGIDSSVTASLLVRALG
CCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHC
KDKVLALIMPYKTTPPEDVKDAIQLAQMLGVKYDVVNIDPIRASFSSTIPAFKESEIVAN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCCCCEEEC
GNILARIRMTILYYYANLNNMIVAGTGDKSELLIGYFTKYGDGGVDILPIGDVYKSQVRM
CCCHHHHHHHHHHHEECCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHH
LGRRLGLPDSIVTKPSSPRLWEGQTAEGELGVSYADIDVILHALVDLRMNPQKAQEATGK
HHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
PLNLIEQVWRRVVTTEHKRRTPVVPRIGLRTIGLDWRMPVHYNGY
CHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEECCCEECCEEECCC
>Mature Secondary Structure
MQPWITLDRILNVNYDYIERRIVDFIRTYVNSVGAKGAVIGLSGGIDSSVTASLLVRALG
CCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHC
KDKVLALIMPYKTTPPEDVKDAIQLAQMLGVKYDVVNIDPIRASFSSTIPAFKESEIVAN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCCCCEEEC
GNILARIRMTILYYYANLNNMIVAGTGDKSELLIGYFTKYGDGGVDILPIGDVYKSQVRM
CCCHHHHHHHHHHHEECCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHH
LGRRLGLPDSIVTKPSSPRLWEGQTAEGELGVSYADIDVILHALVDLRMNPQKAQEATGK
HHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
PLNLIEQVWRRVVTTEHKRRTPVVPRIGLRTIGLDWRMPVHYNGY
CHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEECCCEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA