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The map label for this gene is trpB [H]

Identifier: 159041613

GI number: 159041613

Start: 1096453

End: 1097703

Strand: Reverse

Name: trpB [H]

Synonym: Cmaq_1046

Alternate gene names: 159041613

Gene position: 1097703-1096453 (Counterclockwise)

Preceding gene: 159041614

Following gene: 159041612

Centisome position: 52.84

GC content: 49.24

Gene sequence:

>1251_bases
GTGAGTGGTGTTCCAACTCATTGGTATAACATAGCCGTTGACCTACCTAAACCACTACCACCACCCATGGATCCCTATGA
GGGTGAGTCCAGGATAGCTTTACTTAATAGGATAATGCCGAGTGAATTACTTCACCAGGAATTCACCTCATTAATGTACG
TACCCATTCCCGATGAGGTTAGGGAAATATACATGAGGCTTGGTAGACCAACACCACTGAGGAGGGCTAGGGGTTTGGAG
AAGCTTATAGGTAATGGGGTTAAGATCTACTATAAATTCGAGGGTGCGTTACCGGGTGGTTCACATAAACTCAACACAGC
AGTGCCTCAGGTGTATTATGCGTCTAAGGATGGGGCTAAGGAGGTTGCCACTGAGACTGGGGCTGGGCAATGGGGCTTAG
CAGTATCCATTGCGGCTGCGTTAATTGGGATTAAGGCCGTGGTCTTCATGACCAAGAGCTCCTACTTGAGTAAGAGGCAG
AGGGTTATGTTCATGAGGACTTACGGGGCCATGGTTTACCCAAGCCCCAGTGAGGTTACTAAGTACGGAGTTGAGGCCGT
TAAGGCTAACCCCAATCACCCAGGTAGCTTAGGCTTAGCCATAAGTGAGGCAATAGACTACGTGCTTCAGGGTGAGGGGA
GGAGGTATATTCCAGGTAGTGTGCTTGAATTCGTCCTAATGCATCAAACAGTTATCGGGCAGGAGGCCATTAATCAAATA
CCCGAGGAACCTGACCTAGTGGTTGGATGCGTTGGCGGTGGAAGCAACTTCGCTGGTTTAACGTACCCATTCATAGGGGC
TAAACTAAGGGGTGAGGGTTTTGAGAAGACTAGGTTCCTTGCAGTTGAATCCAAGGCAGCACCCAAGTTGACTCAAGGCA
GGTATGAATACGACTTCCCTGATTCAGGGGGTTTACTACCCATGATTAGAATGCTAACCCTCGGTCACAATTACGTACCA
CCGCCAAGCCATGCAGCCGGCTTAAGGTACCATGGGGCTGCCCCAAGCCTATCACTACTCGTTAAGGAGGGTGTTGTTGA
GACAAGAGCCTACACGCAGGAGGAGGCTCTTGAGGCGGGTGTATTATTTGCAAGGAGTGAAGGCATTATACCTGCCCCTG
AGTCGTCTCATGCAATAAGGGCTGTTATTGATGAAGCCAGGAGAATGCCTAAGGGCAGTGTAATATTGTTTAACCTATCT
GGGCATGGTTTACTGGATCCTGACGCCTACGAGAAGGTGAACGCATCATGA

Upstream 100 bases:

>100_bases
CTGGGGCTGATGGGGTTGTTGTTGGTTCAGCCTTCATTCAAAGAATGAGTGACCTTAATGATGCGTTAAGCTTCCTTAAG
TGGCTTAGGGGTGGTTTACC

Downstream 100 bases:

>100_bases
GGCCACTCCTAGAGAAGATAGCTAGGGGTCTTGAGTTGAGTCTTGAGGAGGCCTATAACGCCGCCTTAGCCATATTAAAG
ATGGAGGCAGGTGAGGCTGA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSGVPTHWYNIAVDLPKPLPPPMDPYEGESRIALLNRIMPSELLHQEFTSLMYVPIPDEVREIYMRLGRPTPLRRARGLE
KLIGNGVKIYYKFEGALPGGSHKLNTAVPQVYYASKDGAKEVATETGAGQWGLAVSIAAALIGIKAVVFMTKSSYLSKRQ
RVMFMRTYGAMVYPSPSEVTKYGVEAVKANPNHPGSLGLAISEAIDYVLQGEGRRYIPGSVLEFVLMHQTVIGQEAINQI
PEEPDLVVGCVGGGSNFAGLTYPFIGAKLRGEGFEKTRFLAVESKAAPKLTQGRYEYDFPDSGGLLPMIRMLTLGHNYVP
PPSHAAGLRYHGAAPSLSLLVKEGVVETRAYTQEEALEAGVLFARSEGIIPAPESSHAIRAVIDEARRMPKGSVILFNLS
GHGLLDPDAYEKVNAS

Sequences:

>Translated_416_residues
MSGVPTHWYNIAVDLPKPLPPPMDPYEGESRIALLNRIMPSELLHQEFTSLMYVPIPDEVREIYMRLGRPTPLRRARGLE
KLIGNGVKIYYKFEGALPGGSHKLNTAVPQVYYASKDGAKEVATETGAGQWGLAVSIAAALIGIKAVVFMTKSSYLSKRQ
RVMFMRTYGAMVYPSPSEVTKYGVEAVKANPNHPGSLGLAISEAIDYVLQGEGRRYIPGSVLEFVLMHQTVIGQEAINQI
PEEPDLVVGCVGGGSNFAGLTYPFIGAKLRGEGFEKTRFLAVESKAAPKLTQGRYEYDFPDSGGLLPMIRMLTLGHNYVP
PPSHAAGLRYHGAAPSLSLLVKEGVVETRAYTQEEALEAGVLFARSEGIIPAPESSHAIRAVIDEARRMPKGSVILFNLS
GHGLLDPDAYEKVNAS
>Mature_415_residues
SGVPTHWYNIAVDLPKPLPPPMDPYEGESRIALLNRIMPSELLHQEFTSLMYVPIPDEVREIYMRLGRPTPLRRARGLEK
LIGNGVKIYYKFEGALPGGSHKLNTAVPQVYYASKDGAKEVATETGAGQWGLAVSIAAALIGIKAVVFMTKSSYLSKRQR
VMFMRTYGAMVYPSPSEVTKYGVEAVKANPNHPGSLGLAISEAIDYVLQGEGRRYIPGSVLEFVLMHQTVIGQEAINQIP
EEPDLVVGCVGGGSNFAGLTYPFIGAKLRGEGFEKTRFLAVESKAAPKLTQGRYEYDFPDSGGLLPMIRMLTLGHNYVPP
PSHAAGLRYHGAAPSLSLLVKEGVVETRAYTQEEALEAGVLFARSEGIIPAPESSHAIRAVIDEARRMPKGSVILFNLSG
HGLLDPDAYEKVNAS

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=357, Percent_Identity=33.8935574229692, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321412, Length=376, Percent_Identity=31.6489361702128, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 45240; Mature: 45109

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVPTHWYNIAVDLPKPLPPPMDPYEGESRIALLNRIMPSELLHQEFTSLMYVPIPDEV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHEEEECCCHHH
REIYMRLGRPTPLRRARGLEKLIGNGVKIYYKFEGALPGGSHKLNTAVPQVYYASKDGAK
HHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHH
EVATETGAGQWGLAVSIAAALIGIKAVVFMTKSSYLSKRQRVMFMRTYGAMVYPSPSEVT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEECCCHHHHH
KYGVEAVKANPNHPGSLGLAISEAIDYVLQGEGRRYIPGSVLEFVLMHQTVIGQEAINQI
HHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PEEPDLVVGCVGGGSNFAGLTYPFIGAKLRGEGFEKTRFLAVESKAAPKLTQGRYEYDFP
CCCCCEEEEEECCCCCCCCCCHHHHCCEECCCCCCCEEEEEEECCCCCCHHCCCEEECCC
DSGGLLPMIRMLTLGHNYVPPPSHAAGLRYHGAAPSLSLLVKEGVVETRAYTQEEALEAG
CCCCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHCHHHHHHHHHHHHHHHC
VLFARSEGIIPAPESSHAIRAVIDEARRMPKGSVILFNLSGHGLLDPDAYEKVNAS
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SGVPTHWYNIAVDLPKPLPPPMDPYEGESRIALLNRIMPSELLHQEFTSLMYVPIPDEV
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHEEEECCCHHH
REIYMRLGRPTPLRRARGLEKLIGNGVKIYYKFEGALPGGSHKLNTAVPQVYYASKDGAK
HHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHH
EVATETGAGQWGLAVSIAAALIGIKAVVFMTKSSYLSKRQRVMFMRTYGAMVYPSPSEVT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEECCCHHHHH
KYGVEAVKANPNHPGSLGLAISEAIDYVLQGEGRRYIPGSVLEFVLMHQTVIGQEAINQI
HHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PEEPDLVVGCVGGGSNFAGLTYPFIGAKLRGEGFEKTRFLAVESKAAPKLTQGRYEYDFP
CCCCCEEEEEECCCCCCCCCCHHHHCCEECCCCCCCEEEEEEECCCCCCHHCCCEEECCC
DSGGLLPMIRMLTLGHNYVPPPSHAAGLRYHGAAPSLSLLVKEGVVETRAYTQEEALEAG
CCCCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHCHHHHHHHHHHHHHHHC
VLFARSEGIIPAPESSHAIRAVIDEARRMPKGSVILFNLSGHGLLDPDAYEKVNAS
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]