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The map label for this gene is ilvB [H]

Identifier: 159041554

GI number: 159041554

Start: 1034978

End: 1036639

Strand: Reverse

Name: ilvB [H]

Synonym: Cmaq_0984

Alternate gene names: 159041554

Gene position: 1036639-1034978 (Counterclockwise)

Preceding gene: 159041556

Following gene: 159041551

Centisome position: 49.9

GC content: 42.36

Gene sequence:

>1662_bases
GTGCGTGATCTTGTTTCAGAATGGTTAATAAATAATGTTGGTAATTACGTGTTTACAGTTGCCGCTGAAAATATTCTGGG
TTTATTAAGGTCTTTAATCAATAGGGGAGGGTTCATTATTAATTCTAAGTTTGAGCCTTCAGCAGGCTTCATGGCATTAG
TTTCATCAAGGCTCCTACTTAAGCCCAGTACACTAATCGTTACCGCTGGTCCAGGGATATTTGGTGCATTATCACCCATT
GCTGAGGCATTCATTGAGGGTGATCCATTAATTGTCATTGCCACATCCATAATTGGTGGTAAGGGTACACGCATGCATCA
AATACGTGACGACACTCAATTAAACACGTTAAGTAATGTTGTTAAGGCATCCCTTAGAGTAAGTAATCCAAGTAACGTTA
CTGAGGTTTTAACTAAGGCGTATAGAATTGCAACAAGTGGTAAACCCGGCCCAGTTTACATTGATATACCATCCGACTTA
ATGGACTTAGACACCACCGGTGAACGTAAGGATGTAAACCCTGTTACTGGGGAAACAGTAGCAATCAATAATGATGCCGT
TAAGGAAGCAGCCAACCTACTCTCCAATGCTGAATTACCTGTCCTACTCCTAGGTAGGGGTGTTATCTTATCCGGCGCTA
AGGATTTGGCGATTAAATTGGCTGAATTACTGAATGCGCCAATCACAACAACAATAATGGCTAAGGGGTTAATATCACCT
AATCATCCCCTCTACGCCGGTGTAGCCGCTGGTAAGGCAGGTAACATGACTGCCTATGAGATTATTAAGAAAGCTGACGT
AGTGCTTGCTATTGGTAATAGATTCAGTGAAATTGGCACTGGTAGGTATAGTCTTGAGATTAGGGGTAAGCTCATTCACG
TTAACATTGATGAGTATGATTTAGGAAGGGCGTATGAACCATATTTAAGGATACGGGCTGATGCTAAGGAATTCCTCCTA
ATGCTAATTAATGTACTTAAGGGCATGAGTATTAGACGTAGAGGTGGTGTAATTAATGAATTAAGGGAATTATGGAGTAT
TGAGAATAGGGAGCTTAATAGTTACTACGAGAAGGCTACAGGCTTAATTAAGCCCTGGGAGGTTATTAGGGCGGTTAGGG
ATGTTTTCAACAAGGGTGGATCAATATTCATTGGTGATGTTGGGGCTCACAGGATTGAAAGCTTCCTAATGCCCATAAAC
GAGAATGAACACTACGTAACAAGCACCAGCTACGTCTCAATGGGGTTAGCAGTACCTGGCGCCGTGGCTGCATCAATAAT
ATACCGTGACAGGGATGTTGTTGCCTTGGTTGGTGATGGTGGATTCCTAATGACTGGGCTTGAGGTTGCAACTGCTGTTC
AATATGGGGTTAAGCCCAAGATAATTGTATTCAATGATTCAGCGTACAGGGTATTGAGGATTTACGAGAAGGTGAGGTTC
AGTAATGCAACCGAATCATTAATTAAACTACCCAGCATTGACTTCAACCTATTAGCCGAATCCCTGGGTGCGGAGGGTTT
AAGGATAACACGTAGGGAGGAGTTGAGGCCGTTGTTGAATGAGGCTTTAAACAGTGACAAGGCCACAATCGTAGATGTGC
ACATTGATCCTAACACAATCCCAATACCGTACCAGAGGTTATACGGTTTAAACACCATTTAA

Upstream 100 bases:

>100_bases
ATTATTATTCATAATTCATGGATTAACAGAGCGGGCGCACTCATGAGCTTTGTTAATACTTATATTTCCTTATAGTTTAG
TATCACTCATGGGTGTGAAG

Downstream 100 bases:

>100_bases
ATGCAGTATACTCTAATACTTACCGTAAAACGCCTGCCTCAGTGTTGATCCTATTAACCCCACTACCACCTTCGCGTGGG
GTATGGGCATGTTGTAGAAG

Product: thiamine pyrophosphate protein central region

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRDLVSEWLINNVGNYVFTVAAENILGLLRSLINRGGFIINSKFEPSAGFMALVSSRLLLKPSTLIVTAGPGIFGALSPI
AEAFIEGDPLIVIATSIIGGKGTRMHQIRDDTQLNTLSNVVKASLRVSNPSNVTEVLTKAYRIATSGKPGPVYIDIPSDL
MDLDTTGERKDVNPVTGETVAINNDAVKEAANLLSNAELPVLLLGRGVILSGAKDLAIKLAELLNAPITTTIMAKGLISP
NHPLYAGVAAGKAGNMTAYEIIKKADVVLAIGNRFSEIGTGRYSLEIRGKLIHVNIDEYDLGRAYEPYLRIRADAKEFLL
MLINVLKGMSIRRRGGVINELRELWSIENRELNSYYEKATGLIKPWEVIRAVRDVFNKGGSIFIGDVGAHRIESFLMPIN
ENEHYVTSTSYVSMGLAVPGAVAASIIYRDRDVVALVGDGGFLMTGLEVATAVQYGVKPKIIVFNDSAYRVLRIYEKVRF
SNATESLIKLPSIDFNLLAESLGAEGLRITRREELRPLLNEALNSDKATIVDVHIDPNTIPIPYQRLYGLNTI

Sequences:

>Translated_553_residues
MRDLVSEWLINNVGNYVFTVAAENILGLLRSLINRGGFIINSKFEPSAGFMALVSSRLLLKPSTLIVTAGPGIFGALSPI
AEAFIEGDPLIVIATSIIGGKGTRMHQIRDDTQLNTLSNVVKASLRVSNPSNVTEVLTKAYRIATSGKPGPVYIDIPSDL
MDLDTTGERKDVNPVTGETVAINNDAVKEAANLLSNAELPVLLLGRGVILSGAKDLAIKLAELLNAPITTTIMAKGLISP
NHPLYAGVAAGKAGNMTAYEIIKKADVVLAIGNRFSEIGTGRYSLEIRGKLIHVNIDEYDLGRAYEPYLRIRADAKEFLL
MLINVLKGMSIRRRGGVINELRELWSIENRELNSYYEKATGLIKPWEVIRAVRDVFNKGGSIFIGDVGAHRIESFLMPIN
ENEHYVTSTSYVSMGLAVPGAVAASIIYRDRDVVALVGDGGFLMTGLEVATAVQYGVKPKIIVFNDSAYRVLRIYEKVRF
SNATESLIKLPSIDFNLLAESLGAEGLRITRREELRPLLNEALNSDKATIVDVHIDPNTIPIPYQRLYGLNTI
>Mature_553_residues
MRDLVSEWLINNVGNYVFTVAAENILGLLRSLINRGGFIINSKFEPSAGFMALVSSRLLLKPSTLIVTAGPGIFGALSPI
AEAFIEGDPLIVIATSIIGGKGTRMHQIRDDTQLNTLSNVVKASLRVSNPSNVTEVLTKAYRIATSGKPGPVYIDIPSDL
MDLDTTGERKDVNPVTGETVAINNDAVKEAANLLSNAELPVLLLGRGVILSGAKDLAIKLAELLNAPITTTIMAKGLISP
NHPLYAGVAAGKAGNMTAYEIIKKADVVLAIGNRFSEIGTGRYSLEIRGKLIHVNIDEYDLGRAYEPYLRIRADAKEFLL
MLINVLKGMSIRRRGGVINELRELWSIENRELNSYYEKATGLIKPWEVIRAVRDVFNKGGSIFIGDVGAHRIESFLMPIN
ENEHYVTSTSYVSMGLAVPGAVAASIIYRDRDVVALVGDGGFLMTGLEVATAVQYGVKPKIIVFNDSAYRVLRIYEKVRF
SNATESLIKLPSIDFNLLAESLGAEGLRITRREELRPLLNEALNSDKATIVDVHIDPNTIPIPYQRLYGLNTI

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=538, Percent_Identity=26.0223048327138, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI21361361, Length=553, Percent_Identity=22.7848101265823, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1790104, Length=500, Percent_Identity=28.6, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=26.5017667844523, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786717, Length=475, Percent_Identity=25.0526315789474, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788716, Length=503, Percent_Identity=24.0556660039761, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1787096, Length=423, Percent_Identity=23.4042553191489, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=504, Percent_Identity=21.8253968253968, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17531301, Length=504, Percent_Identity=21.8253968253968, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=27.0462633451957, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323163, Length=471, Percent_Identity=21.2314225053079, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=22.0588235294118, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=335, Percent_Identity=23.2835820895522, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=335, Percent_Identity=22.6865671641791, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=568, Percent_Identity=23.7676056338028, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60274; Mature: 60274

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLVSEWLINNVGNYVFTVAAENILGLLRSLINRGGFIINSKFEPSAGFMALVSSRLLL
CHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCEE
KPSTLIVTAGPGIFGALSPIAEAFIEGDPLIVIATSIIGGKGTRMHQIRDDTQLNTLSNV
CCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEHHHCCCHHHHHHHHH
VKASLRVSNPSNVTEVLTKAYRIATSGKPGPVYIDIPSDLMDLDTTGERKDVNPVTGETV
HHHHEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCEE
AINNDAVKEAANLLSNAELPVLLLGRGVILSGAKDLAIKLAELLNAPITTTIMAKGLISP
EECCHHHHHHHHHHCCCCCCEEEECCCEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
NHPLYAGVAAGKAGNMTAYEIIKKADVVLAIGNRFSEIGTGRYSLEIRGKLIHVNIDEYD
CCCEEEEEECCCCCCCHHHHHHHHCCEEEEECCHHHHHCCCEEEEEEEEEEEEEECCCCC
LGRAYEPYLRIRADAKEFLLMLINVLKGMSIRRRGGVINELRELWSIENRELNSYYEKAT
CCCCCCCHHEEECCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHC
GLIKPWEVIRAVRDVFNKGGSIFIGDVGAHRIESFLMPINENEHYVTSTSYVSMGLAVPG
CCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCCEEEECHHEEECCCCCH
AVAASIIYRDRDVVALVGDGGFLMTGLEVATAVQYGVKPKIIVFNDSAYRVLRIYEKVRF
HHHHHHEEECCCEEEEEECCCEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
SNATESLIKLPSIDFNLLAESLGAEGLRITRREELRPLLNEALNSDKATIVDVHIDPNTI
CCHHHHHHHCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
PIPYQRLYGLNTI
CCCHHHHCCCCCC
>Mature Secondary Structure
MRDLVSEWLINNVGNYVFTVAAENILGLLRSLINRGGFIINSKFEPSAGFMALVSSRLLL
CHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCEE
KPSTLIVTAGPGIFGALSPIAEAFIEGDPLIVIATSIIGGKGTRMHQIRDDTQLNTLSNV
CCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEHHHCCCHHHHHHHHH
VKASLRVSNPSNVTEVLTKAYRIATSGKPGPVYIDIPSDLMDLDTTGERKDVNPVTGETV
HHHHEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCEE
AINNDAVKEAANLLSNAELPVLLLGRGVILSGAKDLAIKLAELLNAPITTTIMAKGLISP
EECCHHHHHHHHHHCCCCCCEEEECCCEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
NHPLYAGVAAGKAGNMTAYEIIKKADVVLAIGNRFSEIGTGRYSLEIRGKLIHVNIDEYD
CCCEEEEEECCCCCCCHHHHHHHHCCEEEEECCHHHHHCCCEEEEEEEEEEEEEECCCCC
LGRAYEPYLRIRADAKEFLLMLINVLKGMSIRRRGGVINELRELWSIENRELNSYYEKAT
CCCCCCCHHEEECCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHC
GLIKPWEVIRAVRDVFNKGGSIFIGDVGAHRIESFLMPINENEHYVTSTSYVSMGLAVPG
CCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCCEEEECHHEEECCCCCH
AVAASIIYRDRDVVALVGDGGFLMTGLEVATAVQYGVKPKIIVFNDSAYRVLRIYEKVRF
HHHHHHEEECCCEEEEEECCCEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
SNATESLIKLPSIDFNLLAESLGAEGLRITRREELRPLLNEALNSDKATIVDVHIDPNTI
CCHHHHHHHCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
PIPYQRLYGLNTI
CCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]