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The map label for this gene is folE

Identifier: 159041104

GI number: 159041104

Start: 554271

End: 554837

Strand: Reverse

Name: folE

Synonym: Cmaq_0521

Alternate gene names: 159041104

Gene position: 554837-554271 (Counterclockwise)

Preceding gene: 159041105

Following gene: 159041103

Centisome position: 26.71

GC content: 45.5

Gene sequence:

>567_bases
ATGGGTGTTAATAAGGCCTTGATTAAGCAGGCAGTTAAGCAGATACTTGTAGCCATTGGTGAGGACCCTGAGAGAGAGGG
GTTGAGGAGGACGCCTGATAGGGTTGCCAACATGCTTGAGGAGTTGACTAACGGCCGTGAGGAAAATGTCCAGTACACTC
TCTTTGAGGGGTCTAGTAACATGGTTATTGTGGCTGGTATAAGGTTCTCAACACTCTGTGAGCATCACCTACTGCCAATG
CTTGGGGTGACTCACGTAGCATACATACCTAGTGATAAGGTTATTGGAGTGAGTAAAATACCTAGAATAGTGGTTAAGTA
CTCCAGGATGCTTCAACTACAGGAGAGGTTGACTAGGCAGATAATGAATGAGGTTTCCTCAGCCACAGGTTCTGGGGATG
TCATGGTTCTCACCGAGGCATACCATACCTGCATGATGATTAGAGGTGTTAGGAGTGCATCACCCCTTGTTTCAATGGCC
TATAAGGGTAAGTTCAATGATTCCTCACTTAGACTAGAGTTCCTGGAGTACATTAGGCCATTTAGGCTTAATAAATTCCT
CACATGA

Upstream 100 bases:

>100_bases
AGGCTAAGAGGAGGGAATTACTTAAACTCGCTGAGCAGTAATAGATTGAAATTCATTTGCTGGTGAGTTTTTTAAAGAAA
TACTGTGAAGGCTTGATTCT

Downstream 100 bases:

>100_bases
GTAATCTAATTCACCTATTTCAATCGAGTAAGGTATTTTAATAACCCGCATGAGTGTTAGACGTATGATTAACGAGTACC
TGAAGCTAATGAAACCCCAC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MGVNKALIKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSNMVIVAGIRFSTLCEHHLLPM
LGVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQIMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMA
YKGKFNDSSLRLEFLEYIRPFRLNKFLT

Sequences:

>Translated_188_residues
MGVNKALIKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSNMVIVAGIRFSTLCEHHLLPM
LGVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQIMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMA
YKGKFNDSSLRLEFLEYIRPFRLNKFLT
>Mature_187_residues
GVNKALIKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSNMVIVAGIRFSTLCEHHLLPML
GVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQIMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMAY
KGKFNDSSLRLEFLEYIRPFRLNKFLT

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=42.0454545454545, Blast_Score=130, Evalue=6e-31,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=42.0454545454545, Blast_Score=130, Evalue=6e-31,
Organism=Homo sapiens, GI66932970, Length=156, Percent_Identity=39.7435897435897, Blast_Score=113, Evalue=9e-26,
Organism=Homo sapiens, GI66932972, Length=135, Percent_Identity=42.2222222222222, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1788476, Length=183, Percent_Identity=36.0655737704918, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560486, Length=175, Percent_Identity=41.1428571428571, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321706, Length=175, Percent_Identity=42.2857142857143, Blast_Score=128, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24656782, Length=171, Percent_Identity=38.5964912280702, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24656796, Length=171, Percent_Identity=38.5964912280702, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24656791, Length=171, Percent_Identity=38.5964912280702, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CALMQ (A8MC60)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540356.1
- ProteinModelPortal:   A8MC60
- SMR:   A8MC60
- GeneID:   5709170
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0521
- HOGENOM:   HBG370191
- OMA:   MYVEEIF
- ProtClustDB:   CLSK964527
- BioCyc:   CMAQ397948:CMAQ_0521-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21219; Mature: 21088

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVNKALIKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSN
CCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
MVIVAGIRFSTLCEHHLLPMLGVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQ
EEEEECCHHHHHHHHHHHHHHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMAYKGKFNDSSLRLEFLEYIRP
HHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
FRLNKFLT
HHHHHCCC
>Mature Secondary Structure 
GVNKALIKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSN
CCCHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
MVIVAGIRFSTLCEHHLLPMLGVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQ
EEEEECCHHHHHHHHHHHHHHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMAYKGKFNDSSLRLEFLEYIRP
HHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
FRLNKFLT
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA