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The map label for this gene is nirB [C]

Identifier: 159040876

GI number: 159040876

Start: 328555

End: 329991

Strand: Reverse

Name: nirB [C]

Synonym: Cmaq_0290

Alternate gene names: 159040876

Gene position: 329991-328555 (Counterclockwise)

Preceding gene: 159040877

Following gene: 159040875

Centisome position: 15.88

GC content: 45.79

Gene sequence:

>1437_bases
GTGTTCAAGTATTCTGATGTACATAAGTTGGTTTACCCAAGGAGGCATGAGGGGGTTTACAAGAATCGTGGTGATAACCC
ATGGGTCTCGGTGAGGATTAGGCAGAGTATTGGCCGTGACCCAAGTAAGTGGTATTCAAGCCAATTAAGGGTTATTGCAG
AGGTTAGTAGGAGGTATGGTGATGGTAGGGTAATGCTTGGGACCAGGGGTGATGTTGAGATCTTTAAAATCGATTACAGG
CTTTTCGATGAAGTTACGGCTAAGCTTAAGGATACTGGACTTGACCCAAGGGACTCCTGCGGCAATAGCATCAGGAATCC
AATACCATGCCCCAGTAACCACTGCCCACTGGCCCACGTGAATGCCGAGGCCTTATCAGTATTCATAGGGGATTACTTCA
GGTATAAGGGTGAGTATGAGGGGTCGGCATTACCGCATAGGATTAAGATATCGATTTCAGCCTGTGAGAGGGCCTGTGCA
GTACCTGTGAGCCAGGATGTGGGAATTGTGGCTAAGCCTAATGGTAAATTCAACATATACCTAGGCGGGGGTATTGGTGA
ACATGCATTCACAGCTAAATTAGCCTTCAGTGACGTGGAACTGGAGGATATACTACCGTTAATAGTTGGTGTGGTTGAGG
TCTTTAAGAGGAGTGGGGAGAGGAGGGGTTTTAAATGGTTCATTAAGCTTAATGGATTCGATAAAGTTAAGGAGGAGGTA
TTGGCAGTGGCTAGAGAGGTTAAGTCAAAGCTTCCTAAACCACCCGACGTTAAGCCAACATTCATTAACACCAGGGTTAT
TAAGATGCATTACCCAACGGGCTGGGTTACCGCCAGTGAATTAGATGAGTTAGCGCATGCTGCTGATGAGTATGGTTTAG
GCTACGTAGTGTTGTTTAATAATCAAAGCATCTACATACCCATTAGGGATAATGTTAAGGAGATTAGGGGAATTAATGGT
GAAATTAAGGAACCAGGACCATACTACCCAGAGGGCCCAATAACAGTTGCCTGCGTTGGTAGTGAACTCTGTCCGCCAGG
TCTAATAGACACAACGAGCCTAGGCCGTAAAATACACGTACATATCAGTGGATTAAAGGAGCATATTAGAGTCGGCATTA
GTGGCTGTACCCATGATTGCGGCATGAGCTGGGTCTCAGACATCGGCTTCGAGCCTTTCGGCTTCAAAACAAGGATACTG
GTCACAATAGGGGGTGCGTCAAGGCAATTAGGCTTTATACTAGGCACTATCGACCCCAGTGAAGCTGAGTTAACGGCCAA
GGTTATATTAAGCATGTATGTGAAGTCTAAGGCACCCAACATAAGGGAGTTTGCGGTGAGGACTGGTATTGACGCCATTA
GGGATGAGTTAAGGAGGCAAGTACCATCATTTCAACCACCCAATGATAGTATTACTGCATTATTTGGACCTGATTAA

Upstream 100 bases:

>100_bases
TCGAGGAGTGGGCTAAGAAGTATGGGATAACTGGGAAGCCTGCTCAAGTGACTCAATAGTTAAGTAACCTAGGGATGAGG
GTCATTAAAAGTGATTAATT

Downstream 100 bases:

>100_bases
ATCAAGCATTATCCCCACCCTTTAACCTAAGTCCGCATCCCTTACCCAGTGAAGGTGATTTCACCTCACTTCTTTTAAAA
CATGGTACTTTACTTCAATG

Product: nitrite and sulfite reductase 4Fe-4S subunit

Products: nitrite; reduced ferredoxin; H+

Alternate protein names: Sulfite Reductase; Nitrite And Sulphite Reductase 4Fe-4S Region; Nitrite Reductase Large Subunit; Nitrite Reductase; Precorrin-3B Synthase; Sulfite Reductase Assimilatory-Type; Nitrite And Sulphite Reductase 4Fe-4S Domain Protein; Ferredoxin-Sulfite Reductase; Ferredoxin-Sulfite/Nitrite Reductase; Sulfite Reductase Ferredoxin; Adenylylsulfate Reductase Thioredoxin Dependent; Ferredoxin Nitrite Reductase; Nitrite And Sulphite Reductase 4Fe-4S Subunit; FerredoxiN-Nitrite Reductase; Sulfite Reductase Contains SirA-Like Domain; Assimilatory Nitrite Reductase Large Subunit; Nitrite/Sulfite Reductase Domain-Containing Protein; NAD(P)H-Nitrite Reductase

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MFKYSDVHKLVYPRRHEGVYKNRGDNPWVSVRIRQSIGRDPSKWYSSQLRVIAEVSRRYGDGRVMLGTRGDVEIFKIDYR
LFDEVTAKLKDTGLDPRDSCGNSIRNPIPCPSNHCPLAHVNAEALSVFIGDYFRYKGEYEGSALPHRIKISISACERACA
VPVSQDVGIVAKPNGKFNIYLGGGIGEHAFTAKLAFSDVELEDILPLIVGVVEVFKRSGERRGFKWFIKLNGFDKVKEEV
LAVAREVKSKLPKPPDVKPTFINTRVIKMHYPTGWVTASELDELAHAADEYGLGYVVLFNNQSIYIPIRDNVKEIRGING
EIKEPGPYYPEGPITVACVGSELCPPGLIDTTSLGRKIHVHISGLKEHIRVGISGCTHDCGMSWVSDIGFEPFGFKTRIL
VTIGGASRQLGFILGTIDPSEAELTAKVILSMYVKSKAPNIREFAVRTGIDAIRDELRRQVPSFQPPNDSITALFGPD

Sequences:

>Translated_478_residues
MFKYSDVHKLVYPRRHEGVYKNRGDNPWVSVRIRQSIGRDPSKWYSSQLRVIAEVSRRYGDGRVMLGTRGDVEIFKIDYR
LFDEVTAKLKDTGLDPRDSCGNSIRNPIPCPSNHCPLAHVNAEALSVFIGDYFRYKGEYEGSALPHRIKISISACERACA
VPVSQDVGIVAKPNGKFNIYLGGGIGEHAFTAKLAFSDVELEDILPLIVGVVEVFKRSGERRGFKWFIKLNGFDKVKEEV
LAVAREVKSKLPKPPDVKPTFINTRVIKMHYPTGWVTASELDELAHAADEYGLGYVVLFNNQSIYIPIRDNVKEIRGING
EIKEPGPYYPEGPITVACVGSELCPPGLIDTTSLGRKIHVHISGLKEHIRVGISGCTHDCGMSWVSDIGFEPFGFKTRIL
VTIGGASRQLGFILGTIDPSEAELTAKVILSMYVKSKAPNIREFAVRTGIDAIRDELRRQVPSFQPPNDSITALFGPD
>Mature_478_residues
MFKYSDVHKLVYPRRHEGVYKNRGDNPWVSVRIRQSIGRDPSKWYSSQLRVIAEVSRRYGDGRVMLGTRGDVEIFKIDYR
LFDEVTAKLKDTGLDPRDSCGNSIRNPIPCPSNHCPLAHVNAEALSVFIGDYFRYKGEYEGSALPHRIKISISACERACA
VPVSQDVGIVAKPNGKFNIYLGGGIGEHAFTAKLAFSDVELEDILPLIVGVVEVFKRSGERRGFKWFIKLNGFDKVKEEV
LAVAREVKSKLPKPPDVKPTFINTRVIKMHYPTGWVTASELDELAHAADEYGLGYVVLFNNQSIYIPIRDNVKEIRGING
EIKEPGPYYPEGPITVACVGSELCPPGLIDTTSLGRKIHVHISGLKEHIRVGISGCTHDCGMSWVSDIGFEPFGFKTRIL
VTIGGASRQLGFILGTIDPSEAELTAKVILSMYVKSKAPNIREFAVRTGIDAIRDELRRQVPSFQPPNDSITALFGPD

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.7.7.1

Molecular weight: Translated: 53119; Mature: 53119

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKYSDVHKLVYPRRHEGVYKNRGDNPWVSVRIRQSIGRDPSKWYSSQLRVIAEVSRRYG
CCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHCC
DGRVMLGTRGDVEIFKIDYRLFDEVTAKLKDTGLDPRDSCGNSIRNPIPCPSNHCPLAHV
CCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCHHCCCCCCCCCCCCCEEEE
NAEALSVFIGDYFRYKGEYEGSALPHRIKISISACERACAVPVSQDVGIVAKPNGKFNIY
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCEEECCCCEEEEE
LGGGIGEHAFTAKLAFSDVELEDILPLIVGVVEVFKRSGERRGFKWFIKLNGFDKVKEEV
ECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
LAVAREVKSKLPKPPDVKPTFINTRVIKMHYPTGWVTASELDELAHAADEYGLGYVVLFN
HHHHHHHHHHCCCCCCCCCEEECEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
NQSIYIPIRDNVKEIRGINGEIKEPGPYYPEGPITVACVGSELCPPGLIDTTSLGRKIHV
CCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEHHCCCCEEEE
HISGLKEHIRVGISGCTHDCGMSWVSDIGFEPFGFKTRILVTIGGASRQLGFILGTIDPS
EEECHHHHHHCCCCCCCCHHCHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCC
EAELTAKVILSMYVKSKAPNIREFAVRTGIDAIRDELRRQVPSFQPPNDSITALFGPD
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MFKYSDVHKLVYPRRHEGVYKNRGDNPWVSVRIRQSIGRDPSKWYSSQLRVIAEVSRRYG
CCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHCC
DGRVMLGTRGDVEIFKIDYRLFDEVTAKLKDTGLDPRDSCGNSIRNPIPCPSNHCPLAHV
CCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCHHCCCCCCCCCCCCCEEEE
NAEALSVFIGDYFRYKGEYEGSALPHRIKISISACERACAVPVSQDVGIVAKPNGKFNIY
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCEEECCCCEEEEE
LGGGIGEHAFTAKLAFSDVELEDILPLIVGVVEVFKRSGERRGFKWFIKLNGFDKVKEEV
ECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
LAVAREVKSKLPKPPDVKPTFINTRVIKMHYPTGWVTASELDELAHAADEYGLGYVVLFN
HHHHHHHHHHCCCCCCCCCEEECEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
NQSIYIPIRDNVKEIRGINGEIKEPGPYYPEGPITVACVGSELCPPGLIDTTSLGRKIHV
CCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEHHCCCCEEEE
HISGLKEHIRVGISGCTHDCGMSWVSDIGFEPFGFKTRILVTIGGASRQLGFILGTIDPS
EEECHHHHHHCCCCCCCCHHCHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCC
EAELTAKVILSMYVKSKAPNIREFAVRTGIDAIRDELRRQVPSFQPPNDSITALFGPD
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ammonia; H2O; oxidized ferredoxin

Specific reaction: ammonia + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA