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The map label for this gene is trmD [H]

Identifier: 158425578

GI number: 158425578

Start: 4519577

End: 4520269

Strand: Direct

Name: trmD [H]

Synonym: AZC_3954

Alternate gene names: 158425578

Gene position: 4519577-4520269 (Clockwise)

Preceding gene: 158425577

Following gene: 158425579

Centisome position: 84.17

GC content: 72.15

Gene sequence:

>693_bases
ATGACCTTCCGCGCCACCGTCCTCACCCTCTATCCCGAGATGTTTCCGGGGCCGCTCGGCCATGCGCTGGCCGGCCGCGC
GCTGGCCAACGGGCTGTGGGCGCTGGAGGCGGTGCAGATTCGCGACCATGCGCTGGACAAGCACCGCTCGGTGGACGACA
CGCCGGCCGGCGGCGGGCTCGGCATGGTGATGCGGGCCGATGTGCTCGCCCGCGCCATCGACGCCGTCTCTCCGCCGGAC
GATCCCCGCCCCCGCCTGCTCATGACCCCGCGCGGGCGCCCGCTGACGCAGGCGCGCGTGCGCGACCTCGCCGCGGGGCC
CGGTGCCGTCATCCTCTGCGGGCGGTTCGAGGGGGTGGACGAGCGCATCATCCCCGGCCGCAATCTGGAAGAAGTGTGCG
TGGGCGACGTGGTGCTCTCCGGCGGCGAGGCAGCGGCTCTGCTGACGCTGGATGCCTGCGTGCGGCTGATCCCCGGCGTC
ATGGGCAAGACCGAGAGCGGAGCGGAGGAGAGCTTCTCCGACGGCCTGCTCGAATATCCCCAATACACCCGCCCGCAGCT
CTTCGAGGGCGCTAGCATCCCCGAGGTTCTCACCTCCGGTGATCACGCCAAGATCGCCGCCTGGCGCCGGGCGCAGGCGC
TCGCCGTCACCCGCGAGCGCCGGCCGGACCTTCTCGCCAAGGCGGAAAAGTGA

Upstream 100 bases:

>100_bases
GACACCAATCCCGACGAGACTCCGCCCGAGGACGCCGATCAGGCCTGAGCCTGCCCGCCGTCAAGTTGACGGCACGGGCA
GGCTCCCCCATTCCTGCGCC

Downstream 100 bases:

>100_bases
GGCTGCGCGCCGCGTCCGGTGGGGAAGATGACGCTGCCATGAAGGAAAGGGCATGACATCTGCCCCTTTCTCCTGTATGT
GAGCGCGTCCCTTAACTTAC

Product: tRNA (guanine-N1-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTFRATVLTLYPEMFPGPLGHALAGRALANGLWALEAVQIRDHALDKHRSVDDTPAGGGLGMVMRADVLARAIDAVSPPD
DPRPRLLMTPRGRPLTQARVRDLAAGPGAVILCGRFEGVDERIIPGRNLEEVCVGDVVLSGGEAAALLTLDACVRLIPGV
MGKTESGAEESFSDGLLEYPQYTRPQLFEGASIPEVLTSGDHAKIAAWRRAQALAVTRERRPDLLAKAEK

Sequences:

>Translated_230_residues
MTFRATVLTLYPEMFPGPLGHALAGRALANGLWALEAVQIRDHALDKHRSVDDTPAGGGLGMVMRADVLARAIDAVSPPD
DPRPRLLMTPRGRPLTQARVRDLAAGPGAVILCGRFEGVDERIIPGRNLEEVCVGDVVLSGGEAAALLTLDACVRLIPGV
MGKTESGAEESFSDGLLEYPQYTRPQLFEGASIPEVLTSGDHAKIAAWRRAQALAVTRERRPDLLAKAEK
>Mature_229_residues
TFRATVLTLYPEMFPGPLGHALAGRALANGLWALEAVQIRDHALDKHRSVDDTPAGGGLGMVMRADVLARAIDAVSPPDD
PRPRLLMTPRGRPLTQARVRDLAAGPGAVILCGRFEGVDERIIPGRNLEEVCVGDVVLSGGEAAALLTLDACVRLIPGVM
GKTESGAEESFSDGLLEYPQYTRPQLFEGASIPEVLTSGDHAKIAAWRRAQALAVTRERRPDLLAKAEK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=222, Percent_Identity=44.1441441441441, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 24605; Mature: 24473

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRATVLTLYPEMFPGPLGHALAGRALANGLWALEAVQIRDHALDKHRSVDDTPAGGGL
CCCCHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
GMVMRADVLARAIDAVSPPDDPRPRLLMTPRGRPLTQARVRDLAAGPGAVILCGRFEGVD
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCC
ERIIPGRNLEEVCVGDVVLSGGEAAALLTLDACVRLIPGVMGKTESGAEESFSDGLLEYP
CCCCCCCCHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHCCC
QYTRPQLFEGASIPEVLTSGDHAKIAAWRRAQALAVTRERRPDLLAKAEK
CCCCCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
TFRATVLTLYPEMFPGPLGHALAGRALANGLWALEAVQIRDHALDKHRSVDDTPAGGGL
CCCHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
GMVMRADVLARAIDAVSPPDDPRPRLLMTPRGRPLTQARVRDLAAGPGAVILCGRFEGVD
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCC
ERIIPGRNLEEVCVGDVVLSGGEAAALLTLDACVRLIPGVMGKTESGAEESFSDGLLEYP
CCCCCCCCHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHCCC
QYTRPQLFEGASIPEVLTSGDHAKIAAWRRAQALAVTRERRPDLLAKAEK
CCCCCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]