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The map label for this gene is rpoN

Identifier: 158425549

GI number: 158425549

Start: 4487654

End: 4489198

Strand: Reverse

Name: rpoN

Synonym: AZC_3925

Alternate gene names: 158425549

Gene position: 4489198-4487654 (Counterclockwise)

Preceding gene: 158425550

Following gene: 158425548

Centisome position: 83.6

GC content: 67.83

Gene sequence:

>1545_bases
ATGGCGATGAGCCCAAAGATGGAGTTCCGCCAGAGCCAGTCTCTGGTGATGACGCCGCAGCTGATGCAGGCCATCAAGCT
GCTGCAGCTCTCCAATCTCGAACTGGTCGCCTATGTGGAGGCCGAGCTCGAACGCAATCCGCTGCTGGAGCGGGCGAGCG
AGCCGGAAAGCCCCGAGCACGATCCGCCGAACCCGCAGGAAGAGGCACCCACCCCGCCTGACAGTGGCGCGCCGGTGTCC
GGCGACTGGATGGAAAGCGACATGGGCTCGAGCCGCGAGGCCATCGAGACCCGGCTGGACACCGACCTCGGCAATGTCTT
TCCCGATGATGCGCCGGCCGAGCGCATCGGCGCGGGCAGCGGCAGCGGCTCGTCCATCGAATGGGGCTCGGGCGGCGACC
GGGGCGAGGACTACAATCCGGAAGCCTTCCTCGCTGCCGAGACGACGCTGGCCGACCATCTGGAAGCCCAGCTCTCCGTG
GCGGAGCCCGATCCGGCGCGCCGCCTCATCGGCCTCAACCTCATCGGCCTCATCGACGAGACGGGTTATTTCTCCGGCGA
CCTCGATGCGGTGGCCGAGCAACTGGGCGCCACCCACGATCAGGTGGCCGACGTGCTGCGCGTCATCCAGAGCTTCGAGC
CGTCCGGCGTCGGCGCACGGTCGCTCAGCGAATGCCTGGCCCTGCAATTGCGCGACAAGGATCGCTGCGATCCCGCCATG
CAGGCGCTGCTCGACAATCTGGAACTCCTCGCCCGCCACGACCGCAACGCGCTGAAGCGCATCTGCGGGGTGGACGCGGA
AGACCTCGCGGACATGATCGGCGAGATCCGCCGCCTCGATCCGAAGCCCGGCCTCGCCTATGGCGGCGGCGTCGTCCACC
CGCTGGTGCCGGACGTGTTCGTGCGCGAGGGCTCCGACGGCAGCTGGATCGTGGAACTGAATTCCGAGACGCTGCCGCGC
GTGCTGGTGAACCAGACCTATCACGCGACGGTGGCCAAGGCGGCGCGCTCGGCCGAGGAAAAGACCTTCCTCGCCGACTG
CCTCCAGAGCGCCTCCTGGCTTACCCGCTCGCTCGACCAGCGGGCTCGCACCATCCTCAAGGTGGCGAGCGAGATCGTGC
GCCAGCAGGACGCCTTCCTCGTGCACGGCGTGCGGCACCTGCGCCCCCTGAACCTGCGCACGGTGGCGGATGCCATCGGC
ATGCACGAATCCACCGTCTCGCGGGTGACCTCGAACAAGTACATCTCCACCCCGCGCGGGGTGCTGGAGATGAAGTTCTT
CTTCTCCTCCTCCATCGCTTCCTCGGGTGGTGGCGAGGCCCATGCGGCGGAGGCGGTGCGCCACCGCATCAAGAGCCTCA
TCGAGGCCGAGAGTGCGGACGACGTGCTGTCCGACGACACGCTGGTGCAGAAGCTGAAGGACGACGGCATCGATATCGCC
CGCCGAACGGTCGCGAAATATCGCGAGAGCATGAACATCCCGTCCTCGGTCCAGCGCCGCCGCGAAAAGCAGGCCCTGCG
CAGCGACGCCGCCGCCGCCGGCTGA

Upstream 100 bases:

>100_bases
GCGGCGGCTCTATCTCGGCGAAGAGTTCCGGCTGTGAGCACGGCGCTGCGCCTTCCCGCCTCGGCGAGGGCGCGGCGCGC
GAGCGGGGAGGGCTGACGTC

Downstream 100 bases:

>100_bases
GAGGCGAAGATGACCGATCACGAATGTGAAATCCGGTCCGTGGATGGTAGTTGGGAGGCCGTCGGGGTCGAGGAGGCCTT
GGGCTTACCGTCCAGCCTTC

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MAMSPKMEFRQSQSLVMTPQLMQAIKLLQLSNLELVAYVEAELERNPLLERASEPESPEHDPPNPQEEAPTPPDSGAPVS
GDWMESDMGSSREAIETRLDTDLGNVFPDDAPAERIGAGSGSGSSIEWGSGGDRGEDYNPEAFLAAETTLADHLEAQLSV
AEPDPARRLIGLNLIGLIDETGYFSGDLDAVAEQLGATHDQVADVLRVIQSFEPSGVGARSLSECLALQLRDKDRCDPAM
QALLDNLELLARHDRNALKRICGVDAEDLADMIGEIRRLDPKPGLAYGGGVVHPLVPDVFVREGSDGSWIVELNSETLPR
VLVNQTYHATVAKAARSAEEKTFLADCLQSASWLTRSLDQRARTILKVASEIVRQQDAFLVHGVRHLRPLNLRTVADAIG
MHESTVSRVTSNKYISTPRGVLEMKFFFSSSIASSGGGEAHAAEAVRHRIKSLIEAESADDVLSDDTLVQKLKDDGIDIA
RRTVAKYRESMNIPSSVQRRREKQALRSDAAAAG

Sequences:

>Translated_514_residues
MAMSPKMEFRQSQSLVMTPQLMQAIKLLQLSNLELVAYVEAELERNPLLERASEPESPEHDPPNPQEEAPTPPDSGAPVS
GDWMESDMGSSREAIETRLDTDLGNVFPDDAPAERIGAGSGSGSSIEWGSGGDRGEDYNPEAFLAAETTLADHLEAQLSV
AEPDPARRLIGLNLIGLIDETGYFSGDLDAVAEQLGATHDQVADVLRVIQSFEPSGVGARSLSECLALQLRDKDRCDPAM
QALLDNLELLARHDRNALKRICGVDAEDLADMIGEIRRLDPKPGLAYGGGVVHPLVPDVFVREGSDGSWIVELNSETLPR
VLVNQTYHATVAKAARSAEEKTFLADCLQSASWLTRSLDQRARTILKVASEIVRQQDAFLVHGVRHLRPLNLRTVADAIG
MHESTVSRVTSNKYISTPRGVLEMKFFFSSSIASSGGGEAHAAEAVRHRIKSLIEAESADDVLSDDTLVQKLKDDGIDIA
RRTVAKYRESMNIPSSVQRRREKQALRSDAAAAG
>Mature_513_residues
AMSPKMEFRQSQSLVMTPQLMQAIKLLQLSNLELVAYVEAELERNPLLERASEPESPEHDPPNPQEEAPTPPDSGAPVSG
DWMESDMGSSREAIETRLDTDLGNVFPDDAPAERIGAGSGSGSSIEWGSGGDRGEDYNPEAFLAAETTLADHLEAQLSVA
EPDPARRLIGLNLIGLIDETGYFSGDLDAVAEQLGATHDQVADVLRVIQSFEPSGVGARSLSECLALQLRDKDRCDPAMQ
ALLDNLELLARHDRNALKRICGVDAEDLADMIGEIRRLDPKPGLAYGGGVVHPLVPDVFVREGSDGSWIVELNSETLPRV
LVNQTYHATVAKAARSAEEKTFLADCLQSASWLTRSLDQRARTILKVASEIVRQQDAFLVHGVRHLRPLNLRTVADAIGM
HESTVSRVTSNKYISTPRGVLEMKFFFSSSIASSGGGEAHAAEAVRHRIKSLIEAESADDVLSDDTLVQKLKDDGIDIAR
RTVAKYRESMNIPSSVQRRREKQALRSDAAAAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family

Homologues:

Organism=Escherichia coli, GI1789594, Length=507, Percent_Identity=38.6587771203156, Blast_Score=317, Evalue=1e-87,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): RP54_AZOC5 (P33984)

Other databases:

- EMBL:   X69959
- EMBL:   AP009384
- PIR:   S33580
- RefSeq:   YP_001526841.1
- ProteinModelPortal:   P33984
- SMR:   P33984
- GeneID:   5688444
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3925
- HOGENOM:   HBG613158
- OMA:   YKVTESI
- ProtClustDB:   CLSK979133
- BioCyc:   ACAU438753:AZC_3925-MONOMER
- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634
- PRINTS:   PR00045
- TIGRFAMs:   TIGR02395

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD

EC number: NA

Molecular weight: Translated: 55888; Mature: 55757

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00717 SIGMA54_1; PS00718 SIGMA54_2; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSPKMEFRQSQSLVMTPQLMQAIKLLQLSNLELVAYVEAELERNPLLERASEPESPEH
CCCCCCCHHHCCCCEEECHHHHHHHHHHHHCCCEEEEEEHHHHHCCHHHHHCCCCCCCCC
DPPNPQEEAPTPPDSGAPVSGDWMESDMGSSREAIETRLDTDLGNVFPDDAPAERIGAGS
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
GSGSSIEWGSGGDRGEDYNPEAFLAAETTLADHLEAQLSVAEPDPARRLIGLNLIGLIDE
CCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
TGYFSGDLDAVAEQLGATHDQVADVLRVIQSFEPSGVGARSLSECLALQLRDKDRCDPAM
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
QALLDNLELLARHDRNALKRICGVDAEDLADMIGEIRRLDPKPGLAYGGGVVHPLVPDVF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHH
VREGSDGSWIVELNSETLPRVLVNQTYHATVAKAARSAEEKTFLADCLQSASWLTRSLDQ
EEECCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARTILKVASEIVRQQDAFLVHGVRHLRPLNLRTVADAIGMHESTVSRVTSNKYISTPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCH
VLEMKFFFSSSIASSGGGEAHAAEAVRHRIKSLIEAESADDVLSDDTLVQKLKDDGIDIA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHCCHHHH
RRTVAKYRESMNIPSSVQRRREKQALRSDAAAAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AMSPKMEFRQSQSLVMTPQLMQAIKLLQLSNLELVAYVEAELERNPLLERASEPESPEH
CCCCCCHHHCCCCEEECHHHHHHHHHHHHCCCEEEEEEHHHHHCCHHHHHCCCCCCCCC
DPPNPQEEAPTPPDSGAPVSGDWMESDMGSSREAIETRLDTDLGNVFPDDAPAERIGAGS
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
GSGSSIEWGSGGDRGEDYNPEAFLAAETTLADHLEAQLSVAEPDPARRLIGLNLIGLIDE
CCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
TGYFSGDLDAVAEQLGATHDQVADVLRVIQSFEPSGVGARSLSECLALQLRDKDRCDPAM
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
QALLDNLELLARHDRNALKRICGVDAEDLADMIGEIRRLDPKPGLAYGGGVVHPLVPDVF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHH
VREGSDGSWIVELNSETLPRVLVNQTYHATVAKAARSAEEKTFLADCLQSASWLTRSLDQ
EEECCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARTILKVASEIVRQQDAFLVHGVRHLRPLNLRTVADAIGMHESTVSRVTSNKYISTPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCH
VLEMKFFFSSSIASSGGGEAHAAEAVRHRIKSLIEAESADDVLSDDTLVQKLKDDGIDIA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHCCHHHH
RRTVAKYRESMNIPSSVQRRREKQALRSDAAAAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8471796