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The map label for this gene is thlA [H]

Identifier: 158425527

GI number: 158425527

Start: 4455898

End: 4457373

Strand: Reverse

Name: thlA [H]

Synonym: AZC_3903

Alternate gene names: 158425527

Gene position: 4457373-4455898 (Counterclockwise)

Preceding gene: 158425528

Following gene: 158425524

Centisome position: 83.01

GC content: 70.05

Gene sequence:

>1476_bases
TTGACCAGGCGTTGCCATCGGATGCGCCAACCGGTTGGCGCGTGGTGTCCGTGCTCCATCGGCAGGCAAGGAAAGGAAGA
GTCCGCGCAGTTCATAAGGGTATATGCCCGCTCTGATGTGGCTCGCCGGCTCCGTTCGGCTTCCCGACGGCTGCGCGCCC
GCGTCTTGCCAGGGCGAAGCTGCCGCCCGATCGGCCGCTGTGCAGCGGCCCCCGACCAACGGCGCCGGGCCACCCCCGGT
CGATCCGTCCCGCCGTGCCGAAAGGCGCGCTCCGCCCCATTGCAGGAGGTCCTCATGACCCGAGACATCGTCATTGCCCA
TCCGGTCCGCACCGCCATCGGCGCCTATAACGGCACGCTGAAGGGCACCCCCGCCACCGACCTCGGCGCCGTGGTGGTGC
GCGAAACCGTGCGTCGCGCGGGGCTGGATCCGGCTGCGGTCGGGTCCGTGGTGATGGGAAATGTCATCCAGGCGGGGAAC
CGCATGAACCCCGCCCGGCAGGCTTCCATCCATGGCGGGCTGCCGGTGGAGGTGCCCGCCATGACCGTCAATCGGGTCTG
CGGGTCCGGCGCGCAGGCCGTGGTGACTGCAGCGCTGGAGATCGCCTCCGGCCTCCATGATGTGGCTGTGGCCGGCGGCA
TGGAGAATATGGATCGGGCGCCCTACCTCATGGATGGCGGCCGCTGGGGTTATCGGATGGGCCCCGCCCAGATCCTCGAC
AGCATGCTGACGGACGGGCTGAATGATGCCTTCTCCGGCTCCCATTCCGGCTGGCACACGGAAGATCTCGTCGCGCGGTC
CCAGATCACCCGCGCCGAGCAGGATGCCTTTGCGGCGCGCTCGCAGCAGCGCTTCGCGGCGGCCCAGGCCGAAGGCCGGT
TCACCGAGGAGATCGTCGCGGTGGAGGTGAAGGGCCGGAAGGGCATCGAGCTTTTCAGCGTCGACGAGGCGCCGCGCCCC
GACACCACGGTCGAGACGCTGGCCAGGCTGCGGCCCGCCTTCCGGCCCGAGGGCACCATCACGGCCGGCAATGCGCCGGG
TCTCAACAGCGCCGCTGCGGCCATGATCGTCGCCGAGCGCGCCTTTGCCGAGAAGAGTGGCATTGCGCCCCTCGGACGGC
TGGCCGGCTTCGGCATTGCGGGCGTGGAGCCGGGTCTCTTCGGCCTCGGTCCGGTGCCGGCGGTGCGCAAGGCGCTCCAG
CATGCCGGCTGGTCGCTCGGCGACATCGAACGGATCGAGATCAACGAGGCCTTCGCCGCCGTGCCCATCGCGGTGGCCCG
TGAGCTTGGTCTGCCGGAGGACATCGTGAATGTGGAAGGCGGGGCGGTGGCCCATGGCCACCCGATTGGTGCCACTGGCG
CGATCCTCGTGACGCGCCTGCTCCATGCCATGAAGCGTGACGGCGTGAAGCGCGGCGTGGTCACCCTGTGCATCGGCGGC
GGCCAGGGCATCGCGCTGGCGCTCGAAGCGGCGTGA

Upstream 100 bases:

>100_bases
CGCGGGTATATACGCCTATCTGACAGAAGGTGACACGCCGAGGCGTGGACGGCCCGTCGCCGTACCGAACGGTTGGCGTT
TTGTCGTTCGGCGTCGGCGC

Downstream 100 bases:

>100_bases
CGGCTTGAGGCGGCGCCGACTCTAGTTCGTGGAGGCGGCCGATGCCGCCTCTGCGAGCCTGCTGAACTGGAAGACGGGCA
TGGCCTTGCCGAAGAGATAG

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTRRCHRMRQPVGAWCPCSIGRQGKEESAQFIRVYARSDVARRLRSASRRLRARVLPGRSCRPIGRCAAAPDQRRRATPG
RSVPPCRKARSAPLQEVLMTRDIVIAHPVRTAIGAYNGTLKGTPATDLGAVVVRETVRRAGLDPAAVGSVVMGNVIQAGN
RMNPARQASIHGGLPVEVPAMTVNRVCGSGAQAVVTAALEIASGLHDVAVAGGMENMDRAPYLMDGGRWGYRMGPAQILD
SMLTDGLNDAFSGSHSGWHTEDLVARSQITRAEQDAFAARSQQRFAAAQAEGRFTEEIVAVEVKGRKGIELFSVDEAPRP
DTTVETLARLRPAFRPEGTITAGNAPGLNSAAAAMIVAERAFAEKSGIAPLGRLAGFGIAGVEPGLFGLGPVPAVRKALQ
HAGWSLGDIERIEINEAFAAVPIAVARELGLPEDIVNVEGGAVAHGHPIGATGAILVTRLLHAMKRDGVKRGVVTLCIGG
GQGIALALEAA

Sequences:

>Translated_491_residues
MTRRCHRMRQPVGAWCPCSIGRQGKEESAQFIRVYARSDVARRLRSASRRLRARVLPGRSCRPIGRCAAAPDQRRRATPG
RSVPPCRKARSAPLQEVLMTRDIVIAHPVRTAIGAYNGTLKGTPATDLGAVVVRETVRRAGLDPAAVGSVVMGNVIQAGN
RMNPARQASIHGGLPVEVPAMTVNRVCGSGAQAVVTAALEIASGLHDVAVAGGMENMDRAPYLMDGGRWGYRMGPAQILD
SMLTDGLNDAFSGSHSGWHTEDLVARSQITRAEQDAFAARSQQRFAAAQAEGRFTEEIVAVEVKGRKGIELFSVDEAPRP
DTTVETLARLRPAFRPEGTITAGNAPGLNSAAAAMIVAERAFAEKSGIAPLGRLAGFGIAGVEPGLFGLGPVPAVRKALQ
HAGWSLGDIERIEINEAFAAVPIAVARELGLPEDIVNVEGGAVAHGHPIGATGAILVTRLLHAMKRDGVKRGVVTLCIGG
GQGIALALEAA
>Mature_490_residues
TRRCHRMRQPVGAWCPCSIGRQGKEESAQFIRVYARSDVARRLRSASRRLRARVLPGRSCRPIGRCAAAPDQRRRATPGR
SVPPCRKARSAPLQEVLMTRDIVIAHPVRTAIGAYNGTLKGTPATDLGAVVVRETVRRAGLDPAAVGSVVMGNVIQAGNR
MNPARQASIHGGLPVEVPAMTVNRVCGSGAQAVVTAALEIASGLHDVAVAGGMENMDRAPYLMDGGRWGYRMGPAQILDS
MLTDGLNDAFSGSHSGWHTEDLVARSQITRAEQDAFAARSQQRFAAAQAEGRFTEEIVAVEVKGRKGIELFSVDEAPRPD
TTVETLARLRPAFRPEGTITAGNAPGLNSAAAAMIVAERAFAEKSGIAPLGRLAGFGIAGVEPGLFGLGPVPAVRKALQH
AGWSLGDIERIEINEAFAAVPIAVARELGLPEDIVNVEGGAVAHGHPIGATGAILVTRLLHAMKRDGVKRGVVTLCIGGG
QGIALALEAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=44.7300771208226, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=39.9491094147583, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI4557237, Length=392, Percent_Identity=37.5, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=39.2405063291139, Blast_Score=235, Evalue=8e-62,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=31.5294117647059, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=30.1790281329923, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788554, Length=390, Percent_Identity=50.5128205128205, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=44.2159383033419, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=42.8571428571429, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=39.2947103274559, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788683, Length=431, Percent_Identity=30.8584686774942, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=42.2680412371134, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25147385, Length=384, Percent_Identity=36.9791666666667, Blast_Score=255, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=36.8956743002545, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17535917, Length=387, Percent_Identity=30.4909560723514, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=29.9528301886792, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=39.0428211586902, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=34.3511450381679, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=46.3917525773196, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=40.1015228426396, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=37.7892030848329, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17137578, Length=433, Percent_Identity=31.1778290993072, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 51681; Mature: 51550

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRCHRMRQPVGAWCPCSIGRQGKEESAQFIRVYARSDVARRLRSASRRLRARVLPGRS
CCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
CRPIGRCAAAPDQRRRATPGRSVPPCRKARSAPLQEVLMTRDIVIAHPVRTAIGAYNGTL
CCCHHHHCCCCHHHHCCCCCCCCCCHHHHHCCCHHHHHHHCCHHEECHHHHHHHCCCCCC
KGTPATDLGAVVVRETVRRAGLDPAAVGSVVMGNVIQAGNRMNPARQASIHGGLPVEVPA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEECH
MTVNRVCGSGAQAVVTAALEIASGLHDVAVAGGMENMDRAPYLMDGGRWGYRMGPAQILD
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHH
SMLTDGLNDAFSGSHSGWHTEDLVARSQITRAEQDAFAARSQQRFAAAQAEGRFTEEIVA
HHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VEVKGRKGIELFSVDEAPRPDTTVETLARLRPAFRPEGTITAGNAPGLNSAAAAMIVAER
EEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
AFAEKSGIAPLGRLAGFGIAGVEPGLFGLGPVPAVRKALQHAGWSLGDIERIEINEAFAA
HHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECHHHHH
VPIAVARELGLPEDIVNVEGGAVAHGHPIGATGAILVTRLLHAMKRDGVKRGVVTLCIGG
HHHHHHHHCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
GQGIALALEAA
CCCEEEEEECC
>Mature Secondary Structure 
TRRCHRMRQPVGAWCPCSIGRQGKEESAQFIRVYARSDVARRLRSASRRLRARVLPGRS
CCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
CRPIGRCAAAPDQRRRATPGRSVPPCRKARSAPLQEVLMTRDIVIAHPVRTAIGAYNGTL
CCCHHHHCCCCHHHHCCCCCCCCCCHHHHHCCCHHHHHHHCCHHEECHHHHHHHCCCCCC
KGTPATDLGAVVVRETVRRAGLDPAAVGSVVMGNVIQAGNRMNPARQASIHGGLPVEVPA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEECH
MTVNRVCGSGAQAVVTAALEIASGLHDVAVAGGMENMDRAPYLMDGGRWGYRMGPAQILD
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHH
SMLTDGLNDAFSGSHSGWHTEDLVARSQITRAEQDAFAARSQQRFAAAQAEGRFTEEIVA
HHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VEVKGRKGIELFSVDEAPRPDTTVETLARLRPAFRPEGTITAGNAPGLNSAAAAMIVAER
EEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
AFAEKSGIAPLGRLAGFGIAGVEPGLFGLGPVPAVRKALQHAGWSLGDIERIEINEAFAA
HHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECHHHHH
VPIAVARELGLPEDIVNVEGGAVAHGHPIGATGAILVTRLLHAMKRDGVKRGVVTLCIGG
HHHHHHHHCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
GQGIALALEAA
CCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]