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The map label for this gene is rutB [H]

Identifier: 158425524

GI number: 158425524

Start: 4452386

End: 4453234

Strand: Reverse

Name: rutB [H]

Synonym: AZC_3900

Alternate gene names: 158425524

Gene position: 4453234-4452386 (Counterclockwise)

Preceding gene: 158425527

Following gene: 158425523

Centisome position: 82.93

GC content: 69.61

Gene sequence:

>849_bases
GTGGAATATTCGGCAATAGATAGCATCACTCCACCGTGCGTAGTCTCACGGGGTTCGGGAGCGCCGCCTCCCGCCAGCCG
CGAGCCGCGCCCCATGACCTCCGCAGGTTCAGTCACCGCAGGCCTTTCCACGCTCAGCTTCGCGCGCCCCGTCCCCGCGC
CGCGCCCCGTGGCCGTTCCGGCAACGCCGCAAGACGCGGAGATCGACCTCGCGCGCACGGCCCTCGTCATCGTGGACATG
CAGAACGACTTCCTGCATCCGGACGGCTGGTTTCCCCGGCTCGGTGTCGATGCGGCACCGCTTCTGGCCATCGTGCCGCG
CCTGAAGGCACTGGCAGCGGCGGCGCGCGCCGCCGGACTGCCGGTGATCTTCGTCAACTGGGGTGTGCGGGCGGACCGGG
CGGAACTGTCCGCACCCCTCCTCGCCAAGGCTGGCGCCAGCGGCCGGCGGCCGGTCTATGGCGATCCGTCCGCCTCGGGA
CGCGGGCGCATCCTCGTGGAAGGCGACTGGGGGGCGGCCACCATCGACGCGCTGGCGCCCGAGCGCGGCGATCTCGTGGT
GCACAAGCACCGGCTCTCCGGCTTCCATGACAATGAGTTCGACTCGATCCTGCGCAACCGCGGCATCGACACGCTGCTGT
TCAGCGGCGTCAACATTGACCGCTGCGTTTTCTCCACGCTCTGCGACGCGAGCTTCCGCGGCTACGGCTGCCTGCTGGTG
GAGGACGCCTGCGCCACCGTGTCCCCGGCCTATGTGTCGGACGCCATCCTCTTCCTCGTGCGCCAGCTCTACGGCGTCAC
GCTCGCGAGCGATGCCCTCATCGCCGCGCTCGAAGCCCAGCCTCAATGA

Upstream 100 bases:

>100_bases
CGCGGATGGAGCCGGTGCGGGCCACTTCGTCCATGTAGCGCAGGAATCTGAGATGCCGCATGCCGTCTCCACCGGGTTGT
AGCCATTTGGGCTACAATGC

Downstream 100 bases:

>100_bases
CAACCCAGAACCAGACGGGGACCCTCCAGATGACATTCGGAAAACTGACGCGCCGCCTCGCTCTGGGGCTCGCGCTCGGC
ATGGCGTCCAGCGCCTTCGC

Product: isochorismatase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEYSAIDSITPPCVVSRGSGAPPPASREPRPMTSAGSVTAGLSTLSFARPVPAPRPVAVPATPQDAEIDLARTALVIVDM
QNDFLHPDGWFPRLGVDAAPLLAIVPRLKALAAAARAAGLPVIFVNWGVRADRAELSAPLLAKAGASGRRPVYGDPSASG
RGRILVEGDWGAATIDALAPERGDLVVHKHRLSGFHDNEFDSILRNRGIDTLLFSGVNIDRCVFSTLCDASFRGYGCLLV
EDACATVSPAYVSDAILFLVRQLYGVTLASDALIAALEAQPQ

Sequences:

>Translated_282_residues
MEYSAIDSITPPCVVSRGSGAPPPASREPRPMTSAGSVTAGLSTLSFARPVPAPRPVAVPATPQDAEIDLARTALVIVDM
QNDFLHPDGWFPRLGVDAAPLLAIVPRLKALAAAARAAGLPVIFVNWGVRADRAELSAPLLAKAGASGRRPVYGDPSASG
RGRILVEGDWGAATIDALAPERGDLVVHKHRLSGFHDNEFDSILRNRGIDTLLFSGVNIDRCVFSTLCDASFRGYGCLLV
EDACATVSPAYVSDAILFLVRQLYGVTLASDALIAALEAQPQ
>Mature_282_residues
MEYSAIDSITPPCVVSRGSGAPPPASREPRPMTSAGSVTAGLSTLSFARPVPAPRPVAVPATPQDAEIDLARTALVIVDM
QNDFLHPDGWFPRLGVDAAPLLAIVPRLKALAAAARAAGLPVIFVNWGVRADRAELSAPLLAKAGASGRRPVYGDPSASG
RGRILVEGDWGAATIDALAPERGDLVVHKHRLSGFHDNEFDSILRNRGIDTLLFSGVNIDRCVFSTLCDASFRGYGCLLV
EDACATVSPAYVSDAILFLVRQLYGVTLASDALIAALEAQPQ

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=212, Percent_Identity=32.5471698113208, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081992, Length=192, Percent_Identity=30.2083333333333, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 29646; Mature: 29646

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSAIDSITPPCVVSRGSGAPPPASREPRPMTSAGSVTAGLSTLSFARPVPAPRPVAVP
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECC
ATPQDAEIDLARTALVIVDMQNDFLHPDGWFPRLGVDAAPLLAIVPRLKALAAAARAAGL
CCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PVIFVNWGVRADRAELSAPLLAKAGASGRRPVYGDPSASGRGRILVEGDWGAATIDALAP
CEEEEECCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHCCC
ERGDLVVHKHRLSGFHDNEFDSILRNRGIDTLLFSGVNIDRCVFSTLCDASFRGYGCLLV
CCCCEEEEEHHCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEE
EDACATVSPAYVSDAILFLVRQLYGVTLASDALIAALEAQPQ
ECCHHHCCHHHHHHHHHHHHHHHHCCCHHCCHHHEEECCCCC
>Mature Secondary Structure
MEYSAIDSITPPCVVSRGSGAPPPASREPRPMTSAGSVTAGLSTLSFARPVPAPRPVAVP
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECC
ATPQDAEIDLARTALVIVDMQNDFLHPDGWFPRLGVDAAPLLAIVPRLKALAAAARAAGL
CCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PVIFVNWGVRADRAELSAPLLAKAGASGRRPVYGDPSASGRGRILVEGDWGAATIDALAP
CEEEEECCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHCCC
ERGDLVVHKHRLSGFHDNEFDSILRNRGIDTLLFSGVNIDRCVFSTLCDASFRGYGCLLV
CCCCEEEEEHHCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEE
EDACATVSPAYVSDAILFLVRQLYGVTLASDALIAALEAQPQ
ECCHHHCCHHHHHHHHHHHHHHHHCCCHHCCHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA