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The map label for this gene is nolK [H]

Identifier: 158425474

GI number: 158425474

Start: 4403947

End: 4404933

Strand: Direct

Name: nolK [H]

Synonym: AZC_3850

Alternate gene names: 158425474

Gene position: 4403947-4404933 (Clockwise)

Preceding gene: 158425472

Following gene: 158425475

Centisome position: 82.01

GC content: 47.92

Gene sequence:

>987_bases
ATGCGCGAGAGCGAAGGGCTTTACGGCGACAGGGAGGGAATCCCCCGCGTGGGTAAAGGTAAAAAACTCTTGATCACCGG
TGGTCGAGGTATGGTCGGGCGAAACTTGATTGCATGCGCGGCGCGCTCGGGCTGGGAAATAATAGCGCCCACCTCAGTCG
ATCTGGATTTGCGGAATGCAGAGGCGGTCGAGCAATATATCCGTCGTCAGCTACCGGATGTTGTGGTTCACGCCGCTGGC
GTAGTCGGTGGCATCCACGCAAATATCGCCGATCCGATACATTTTCTCGCAGATAATGCGGCGATGGCTCTAAATGTCGT
CATGTCGAGCTTTCGTTCGGAAGTGGTTACATTGATCAACTTGTCCTCGAGTTGCATGTATCCTGCCTGCATCGAGGGGC
CTTTAAAAGAGTGCGACATCCTGCGTGGCCCGTTTGAAGTGACTAATGAAGGATATGCTCTAGCGAAGACGGTTGGTCTT
AAGATTTGTGAATACATTGACAAGCTGCCGAATTTTAATTACAAAACACTGATTGCATGCAATTTATATGGAGTGGGCGA
CAATTTTGATCCAAGAAGATCGCATCTTCTGCCTGCAATAATAGAAAAAATTCACAAAGCCTCTCAATGTGGTAGCGAAA
GTGTGAGTATCTGGGGCGATGGGACTGCTAGGCGTGAGTTTATGTTCGCGTACGATTTTGCAAAAATTATCATTAAGGCA
TTGGAAGTTCCGGAATTAATACCTAGTTCAATGAATGTCGGTGTTGGGAAAGATTTGTCTGTGTTAGAATATTACAGCTT
GGTGGCTCGTGTAATTGGGTGGAGCGGTGAATTTGTCTATGACTTGAATAGGCCTGTGGGTATGCGTAGTAAATTGATGG
ATATCACGCATCTTACGGCATTAGGTTGGGTCCCAGAACGGTCGCTTGAAGGGGGGATTCGATCGACATACCAGTATTAT
ATCACCGGGAATGAGGTCTACGAGTAG

Upstream 100 bases:

>100_bases
GCCATCCATCGGATGGATAATTCGGATAATCAAAAAGGATTTTGTTTTTTGAAATTCAATCACCAGTATCGCGAGAAGTG
GGCATTAACAAAGGTCAGAC

Downstream 100 bases:

>100_bases
AGTCGTTCTATCGGTGTCTCGTGCGGGAATGGGGTGCTCGGTCGGGGAGACCTGAGTGCGCCTTCAGGATTTGACATTCC
CGGTCGATGACGTAGACGGC

Product: nodulation protein nolK

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRESEGLYGDREGIPRVGKGKKLLITGGRGMVGRNLIACAARSGWEIIAPTSVDLDLRNAEAVEQYIRRQLPDVVVHAAG
VVGGIHANIADPIHFLADNAAMALNVVMSSFRSEVVTLINLSSSCMYPACIEGPLKECDILRGPFEVTNEGYALAKTVGL
KICEYIDKLPNFNYKTLIACNLYGVGDNFDPRRSHLLPAIIEKIHKASQCGSESVSIWGDGTARREFMFAYDFAKIIIKA
LEVPELIPSSMNVGVGKDLSVLEYYSLVARVIGWSGEFVYDLNRPVGMRSKLMDITHLTALGWVPERSLEGGIRSTYQYY
ITGNEVYE

Sequences:

>Translated_328_residues
MRESEGLYGDREGIPRVGKGKKLLITGGRGMVGRNLIACAARSGWEIIAPTSVDLDLRNAEAVEQYIRRQLPDVVVHAAG
VVGGIHANIADPIHFLADNAAMALNVVMSSFRSEVVTLINLSSSCMYPACIEGPLKECDILRGPFEVTNEGYALAKTVGL
KICEYIDKLPNFNYKTLIACNLYGVGDNFDPRRSHLLPAIIEKIHKASQCGSESVSIWGDGTARREFMFAYDFAKIIIKA
LEVPELIPSSMNVGVGKDLSVLEYYSLVARVIGWSGEFVYDLNRPVGMRSKLMDITHLTALGWVPERSLEGGIRSTYQYY
ITGNEVYE
>Mature_328_residues
MRESEGLYGDREGIPRVGKGKKLLITGGRGMVGRNLIACAARSGWEIIAPTSVDLDLRNAEAVEQYIRRQLPDVVVHAAG
VVGGIHANIADPIHFLADNAAMALNVVMSSFRSEVVTLINLSSSCMYPACIEGPLKECDILRGPFEVTNEGYALAKTVGL
KICEYIDKLPNFNYKTLIACNLYGVGDNFDPRRSHLLPAIIEKIHKASQCGSESVSIWGDGTARREFMFAYDFAKIIIKA
LEVPELIPSSMNVGVGKDLSVLEYYSLVARVIGWSGEFVYDLNRPVGMRSKLMDITHLTALGWVPERSLEGGIRSTYQYY
ITGNEVYE

Specific function: Probable nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=276, Percent_Identity=29.3478260869565, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=35.126582278481, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17554428, Length=317, Percent_Identity=26.4984227129338, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19922778, Length=315, Percent_Identity=27.3015873015873, Blast_Score=131, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 36116; Mature: 36116

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRESEGLYGDREGIPRVGKGKKLLITGGRGMVGRNLIACAARSGWEIIAPTSVDLDLRNA
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHCHHHHHHCCCCCEEECCCCCCEECCCH
EAVEQYIRRQLPDVVVHAAGVVGGIHANIADPIHFLADNAAMALNVVMSSFRSEVVTLIN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHEEE
LSSSCMYPACIEGPLKECDILRGPFEVTNEGYALAKTVGLKICEYIDKLPNFNYKTLIAC
CCCCCCCHHHHCCCHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
NLYGVGDNFDPRRSHLLPAIIEKIHKASQCGSESVSIWGDGTARREFMFAYDFAKIIIKA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
LEVPELIPSSMNVGVGKDLSVLEYYSLVARVIGWSGEFVYDLNRPVGMRSKLMDITHLTA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
LGWVPERSLEGGIRSTYQYYITGNEVYE
HCCCCCHHHCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRESEGLYGDREGIPRVGKGKKLLITGGRGMVGRNLIACAARSGWEIIAPTSVDLDLRNA
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHCHHHHHHCCCCCEEECCCCCCEECCCH
EAVEQYIRRQLPDVVVHAAGVVGGIHANIADPIHFLADNAAMALNVVMSSFRSEVVTLIN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHEEE
LSSSCMYPACIEGPLKECDILRGPFEVTNEGYALAKTVGLKICEYIDKLPNFNYKTLIAC
CCCCCCCHHHHCCCHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
NLYGVGDNFDPRRSHLLPAIIEKIHKASQCGSESVSIWGDGTARREFMFAYDFAKIIIKA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
LEVPELIPSSMNVGVGKDLSVLEYYSLVARVIGWSGEFVYDLNRPVGMRSKLMDITHLTA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
LGWVPERSLEGGIRSTYQYYITGNEVYE
HCCCCCHHHCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1472718 [H]