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The map label for this gene is vfr [H]

Identifier: 158425412

GI number: 158425412

Start: 4341832

End: 4342593

Strand: Direct

Name: vfr [H]

Synonym: AZC_3788

Alternate gene names: 158425412

Gene position: 4341832-4342593 (Clockwise)

Preceding gene: 158425411

Following gene: 158425415

Centisome position: 80.86

GC content: 69.55

Gene sequence:

>762_bases
ATGGACCCCGCACCCCTCCCCCTCAAGGCCCCCTTGCCCCCTCCCGCCGGCCGGAGCGTCCAAGCGGCACGCATGCCGGC
GAAGGCGGAGGTGCCGCCCGTCGTGGCCCGCTCGTGCCTGTTCCAGGGGCTCACGGGCGATGAGGTCGCCCGCATCTTCG
CGCGTGCCCATCCGCGCAGCTTCACGCGCGACGAACCCGTGATCACCGATGGCGACCTGCCCGAGCGGCTCTATTTCGTC
ACCGAGGGCAGCTTCATCTGTCGCAAGACCTCTCCGGCCGGACTGGTCTTCACCCTGGGCCAGCTTCATCCTGGCAGCTT
CTTTGGGGAAAGCGGCGTGATCGGCGGCCGCCGGAGCGACATGGACGTCACGGCCACGGGGGAAGCTGCGGCGCTCGGGG
TCCCCGCCTCGGACTTTCGCCATGCACTGGAGCGCCATCCCTCCATCAGCCGCCACCTGGTCGATGAAATGGCGGCCCGC
ATCGCCGCACTCACCCTGCTGAGTTTCGAAGTCGCCACGCTGAAGCTGGATGCCCGCCTGAGCCGCGCCATCGCAAAGCT
TGCGCGCGAGCGCGATCAGTTCCGCGACGGGGGACTGATCCGCCCCGCGCCGACCCATGCGGAACTGGCGGCCATGCTCG
GCACCACGCGGGAAGTGGTCTCCCGCTCCATGACCGCCCTCTCCCGCCAGGGCCTCATCGAGACGCGCCGGCAGGAAATC
CTGATCCGCCGGGCCCTTGGCCTGCAGAACTTCGAACGGTGA

Upstream 100 bases:

>100_bases
CCGGTCCGCCGCCAGATCCGCCTGCCGCTTCGGCGCGGGCATCTGCGGCCGGTCGCCGGGCCACGGCTCTCCTGACCCGC
GCCCACCGCCGGAGAGCGCC

Downstream 100 bases:

>100_bases
CGGGGAAGAGGAAGAAGGAGGCGACCATCCCTGAAAAACCAGCGCGCGGGACGGCGCTGTCGGGATGGCTCAGCGCAGCA
CGACGATAAAAAGACGGGGG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDPAPLPLKAPLPPPAGRSVQAARMPAKAEVPPVVARSCLFQGLTGDEVARIFARAHPRSFTRDEPVITDGDLPERLYFV
TEGSFICRKTSPAGLVFTLGQLHPGSFFGESGVIGGRRSDMDVTATGEAAALGVPASDFRHALERHPSISRHLVDEMAAR
IAALTLLSFEVATLKLDARLSRAIAKLARERDQFRDGGLIRPAPTHAELAAMLGTTREVVSRSMTALSRQGLIETRRQEI
LIRRALGLQNFER

Sequences:

>Translated_253_residues
MDPAPLPLKAPLPPPAGRSVQAARMPAKAEVPPVVARSCLFQGLTGDEVARIFARAHPRSFTRDEPVITDGDLPERLYFV
TEGSFICRKTSPAGLVFTLGQLHPGSFFGESGVIGGRRSDMDVTATGEAAALGVPASDFRHALERHPSISRHLVDEMAAR
IAALTLLSFEVATLKLDARLSRAIAKLARERDQFRDGGLIRPAPTHAELAAMLGTTREVVSRSMTALSRQGLIETRRQEI
LIRRALGLQNFER
>Mature_253_residues
MDPAPLPLKAPLPPPAGRSVQAARMPAKAEVPPVVARSCLFQGLTGDEVARIFARAHPRSFTRDEPVITDGDLPERLYFV
TEGSFICRKTSPAGLVFTLGQLHPGSFFGESGVIGGRRSDMDVTATGEAAALGVPASDFRHALERHPSISRHLVDEMAAR
IAALTLLSFEVATLKLDARLSRAIAKLARERDQFRDGGLIRPAPTHAELAAMLGTTREVVSRSMTALSRQGLIETRRQEI
LIRRALGLQNFER

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=192, Percent_Identity=24.4791666666667, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27477; Mature: 27477

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPAPLPLKAPLPPPAGRSVQAARMPAKAEVPPVVARSCLFQGLTGDEVARIFARAHPRS
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
FTRDEPVITDGDLPERLYFVTEGSFICRKTSPAGLVFTLGQLHPGSFFGESGVIGGRRSD
CCCCCCEEECCCCCCEEEEEECCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
MDVTATGEAAALGVPASDFRHALERHPSISRHLVDEMAARIAALTLLSFEVATLKLDARL
CCEEECCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRAIAKLARERDQFRDGGLIRPAPTHAELAAMLGTTREVVSRSMTALSRQGLIETRRQEI
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LIRRALGLQNFER
HHHHHHCHHCCCC
>Mature Secondary Structure
MDPAPLPLKAPLPPPAGRSVQAARMPAKAEVPPVVARSCLFQGLTGDEVARIFARAHPRS
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
FTRDEPVITDGDLPERLYFVTEGSFICRKTSPAGLVFTLGQLHPGSFFGESGVIGGRRSD
CCCCCCEEECCCCCCEEEEEECCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
MDVTATGEAAALGVPASDFRHALERHPSISRHLVDEMAARIAALTLLSFEVATLKLDARL
CCEEECCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRAIAKLARERDQFRDGGLIRPAPTHAELAAMLGTTREVVSRSMTALSRQGLIETRRQEI
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LIRRALGLQNFER
HHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]