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The map label for this gene is nth [H]

Identifier: 158425308

GI number: 158425308

Start: 4231131

End: 4232210

Strand: Direct

Name: nth [H]

Synonym: AZC_3684

Alternate gene names: 158425308

Gene position: 4231131-4232210 (Clockwise)

Preceding gene: 158425305

Following gene: 158425312

Centisome position: 78.8

GC content: 71.11

Gene sequence:

>1080_bases
ATGCCGCGAAAGACGACTGGCTCCGCGCCGCCTCCCGTGCCCGCCAAGGCCACCGGCCGCAAGGCGGCCCCTGTCGCCCC
GTCCGACCAGAAGGAAGCGCCGGCAAAAGCGGCGAAAGCCGGCGCGGGCAAGGAGACCGGCGCCCGCACGGTGATGCCGG
CCAAGGCCAATGCAGGAGCGCCGAAAGCGACCGCTCCGAAGACTGGGGCGTCACTCGCCTCCCCGGCTCCCGCCACGAAG
GCCACTGCCAAGGCTGTGTCGAGCACCAAAGCCGCATCGACCACGAAGCCCCGCAAGGCGCCGGCCAGCACGCGATCCGG
CCTCGCGCCGCCCAAGGGGCTCAAGGCGAAGGCCGAGGCGGCGGGGCTCGCGCCCGGTGCCGCAAAGCCCCGCCGCCGGG
CGGTGGCCAATGCGGCGGCGGCGGTGCACGGGCGCACCGTCACGCCCTGGAGCGACGACGACATCTTCGAGGCCTTCTCC
CGCTTCGAGCGGCTGAACCCGGAGCCGAAGGGCGAGCTGGAATATCACGACCCCTTCACTTTGCTGGTGGCGGTGGTTCT
CTCCGCGCAGGCGACAGACGTCGGCGTCAACAAGGCGACGCGCGGCCTCTTCGCCGCCGCCCCGACGCCCAAGGCCATGT
TCGCGCTCGGCGAGGAAGGGGTCGCGCAGTTCATCCGCACCCTCGGCCTCTATCGCGGCAAGGCGAAGAATGTGGTGGAG
CTGTCCCGCCTGCTGCTGGAGAAGCACGACGGCGTGGTGCCGCCGGACCGCGAGGCACTGGAGGCGCTGCCCGGCGTCGG
TCGCAAGACGGCGAATGTGGTGCTGAACATCGCCTTCGGCCTGCCCACCATTGCGGTGGACACCCACCTCTTCCGCGTCG
CGAACCGCACCGGGCTCGCGCCGGGGGCGACGCCGCTGGACGTGGAGCTGGCGCTCGAAAAGCGCATCCCCGACCGCTTC
AAGCTGCACGCCCACCACTGGCTGATCCTGCACGGGCGCTACATCTGCAAGGCCCTGCGCCCCGACTGTCCCATCTGTCC
CATCAACGACCTCTGCCGCTGGCCGGACAAGCCGATCTGA

Upstream 100 bases:

>100_bases
GCGATGCGGGGCGAGTGTGGCGGCGAAAACCGTGGGCTCCTCGTCCGGAACGACGGGCGGGTCGTTGGCGTCGGTCATGG
CTGCACAGTATACGTGAAAA

Downstream 100 bases:

>100_bases
GCCCGCTCAGGCCGAGACCTTCCGCCCCTCGATCAGCGGCCCCTGCCCCATGCCGGCGCCGGGCACCGGGGCAAGGCGGC
GGTGGAGGTGGACCATCAGC

Product: endonuclease III/Nth precursor

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPRKTTGSAPPPVPAKATGRKAAPVAPSDQKEAPAKAAKAGAGKETGARTVMPAKANAGAPKATAPKTGASLASPAPATK
ATAKAVSSTKAASTTKPRKAPASTRSGLAPPKGLKAKAEAAGLAPGAAKPRRRAVANAAAAVHGRTVTPWSDDDIFEAFS
RFERLNPEPKGELEYHDPFTLLVAVVLSAQATDVGVNKATRGLFAAAPTPKAMFALGEEGVAQFIRTLGLYRGKAKNVVE
LSRLLLEKHDGVVPPDREALEALPGVGRKTANVVLNIAFGLPTIAVDTHLFRVANRTGLAPGATPLDVELALEKRIPDRF
KLHAHHWLILHGRYICKALRPDCPICPINDLCRWPDKPI

Sequences:

>Translated_359_residues
MPRKTTGSAPPPVPAKATGRKAAPVAPSDQKEAPAKAAKAGAGKETGARTVMPAKANAGAPKATAPKTGASLASPAPATK
ATAKAVSSTKAASTTKPRKAPASTRSGLAPPKGLKAKAEAAGLAPGAAKPRRRAVANAAAAVHGRTVTPWSDDDIFEAFS
RFERLNPEPKGELEYHDPFTLLVAVVLSAQATDVGVNKATRGLFAAAPTPKAMFALGEEGVAQFIRTLGLYRGKAKNVVE
LSRLLLEKHDGVVPPDREALEALPGVGRKTANVVLNIAFGLPTIAVDTHLFRVANRTGLAPGATPLDVELALEKRIPDRF
KLHAHHWLILHGRYICKALRPDCPICPINDLCRWPDKPI
>Mature_358_residues
PRKTTGSAPPPVPAKATGRKAAPVAPSDQKEAPAKAAKAGAGKETGARTVMPAKANAGAPKATAPKTGASLASPAPATKA
TAKAVSSTKAASTTKPRKAPASTRSGLAPPKGLKAKAEAAGLAPGAAKPRRRAVANAAAAVHGRTVTPWSDDDIFEAFSR
FERLNPEPKGELEYHDPFTLLVAVVLSAQATDVGVNKATRGLFAAAPTPKAMFALGEEGVAQFIRTLGLYRGKAKNVVEL
SRLLLEKHDGVVPPDREALEALPGVGRKTANVVLNIAFGLPTIAVDTHLFRVANRTGLAPGATPLDVELALEKRIPDRFK
LHAHHWLILHGRYICKALRPDCPICPINDLCRWPDKPI

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=59.4059405940594, Blast_Score=251, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.0734463276836, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 37542; Mature: 37411

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKTTGSAPPPVPAKATGRKAAPVAPSDQKEAPAKAAKAGAGKETGARTVMPAKANAGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHCCCCCCCCCCEEECCCCCCCC
PKATAPKTGASLASPAPATKATAKAVSSTKAASTTKPRKAPASTRSGLAPPKGLKAKAEA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AGLAPGAAKPRRRAVANAAAAVHGRTVTPWSDDDIFEAFSRFERLNPEPKGELEYHDPFT
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LLVAVVLSAQATDVGVNKATRGLFAAAPTPKAMFALGEEGVAQFIRTLGLYRGKAKNVVE
HHHHHHHHHCCCCCCCCHHHCCEEECCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCHHHH
LSRLLLEKHDGVVPPDREALEALPGVGRKTANVVLNIAFGLPTIAVDTHLFRVANRTGLA
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHCCCC
PGATPLDVELALEKRIPDRFKLHAHHWLILHGRYICKALRPDCPICPINDLCRWPDKPI
CCCCCCCHHHHHHHCCCHHEEEEEEEEEEEEHHHHHHHHCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
PRKTTGSAPPPVPAKATGRKAAPVAPSDQKEAPAKAAKAGAGKETGARTVMPAKANAGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHCCCCCCCCCCEEECCCCCCCC
PKATAPKTGASLASPAPATKATAKAVSSTKAASTTKPRKAPASTRSGLAPPKGLKAKAEA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AGLAPGAAKPRRRAVANAAAAVHGRTVTPWSDDDIFEAFSRFERLNPEPKGELEYHDPFT
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LLVAVVLSAQATDVGVNKATRGLFAAAPTPKAMFALGEEGVAQFIRTLGLYRGKAKNVVE
HHHHHHHHHCCCCCCCCHHHCCEEECCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCHHHH
LSRLLLEKHDGVVPPDREALEALPGVGRKTANVVLNIAFGLPTIAVDTHLFRVANRTGLA
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHCCCC
PGATPLDVELALEKRIPDRFKLHAHHWLILHGRYICKALRPDCPICPINDLCRWPDKPI
CCCCCCCHHHHHHHCCCHHEEEEEEEEEEEEHHHHHHHHCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]