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The map label for this gene is aspS

Identifier: 158425283

GI number: 158425283

Start: 4205742

End: 4207514

Strand: Reverse

Name: aspS

Synonym: AZC_3659

Alternate gene names: 158425283

Gene position: 4207514-4205742 (Counterclockwise)

Preceding gene: 158425286

Following gene: 158425282

Centisome position: 78.36

GC content: 66.89

Gene sequence:

>1773_bases
ATGCACCGCTACCGCACCCACACCTGCGGCGCGCTCACCGAAGCCCAGGTGGGCGACACCGTCCGCGTCTCCGGCTGGTG
CCACCGTATCCGCGACCATGGCGGCGTGCTGTTCGTGGACCTGCGCGACCATTACGGCTTGACCCAGGTGGTGTTCGATC
CCGACAGCGCCGCCTTCGCCGCCGCCGAGAAGGTGCGCGCGGAATGGGTGATCCGCGTCGATGGCCGCGTGCGCCTGCGC
CCCGAGGGCACGGAGAATTCAGAGCTCGCCACCGGCAAGGTGGAGATCTACGCCACCGACCTTGAGGTGCTCGGCCCGGC
CGCCGAGCTGCCGCTGCCGGTGTTCGGCGATCAGGATTATCCGGAAGATATCCGCCTGCGCTACCGCTTCCTGGACCTGC
GCCGCGAGCGCATCCACGGCAACATCATGAAGCGCGGCCAGATCATCGACAGCCTGCGCCAGCGCATGAAGGGGCAGGGC
TTCTTCGAGTTCCAGACGCCGATCCTGACCGCCTCCTCGCCGGAAGGCGCGCGCGACTTCCTCGTGCCCTCGCGCATCCA
TCCCGGCAAGTTCTACGCCCTGCCCCAGGCGCCCCAGCAGTACAAGCAGCTCATCATGATGAGCGGCTTCGACCGCTACT
TCCAGATCGCCCCCTGTTTCCGCGACGAGGACCCGCGTGCCGACCGCCTGCCGGGCGAGTTCTACCAGCTCGACCTCGAA
ATGAGCTTCGTCACGCAGGACGACGTGTTCGCGGCGGTGGAGCCGGTGATCCGGGGCGTGTTCGAGGAGTTCGGCGGCGG
CAAGCCCGTCACCCAGAACTGGCCGCGCATTCCATATGCGGAAGCCCTGCGCAAGTACGGCTCCGACAAGCCGGACCTGC
GCAACCCGCTGGTGATGCAGAACGTCTCCGAGCACTTCCGTGGCTCGAATTTCAAGGTGTTCGCGCGCCTGCTCGAAGAC
CCGAAGAACGAGGTGTGGGCGATCCCCGGCCCGACCGGCGGTTCGCGTGCGTTCTGCGACCGCATGAACTCGTGGGCGCA
GGGCGAGGGCCAGCCGGGCCTCGGCTACATCATGTGGCGTGAAGGCGGAGAGGGCGCAGGCCCGCTCGCCAACAACATCG
GCCCCGAGCGCACCGCTGCCATCCGCGAGCAGCTCGGCCTCAAGGACGGCGATGCCGCCTTCTTCGTCGCCGGCAATCCG
GAGAAGTTCTACAAGTTCGCCGGTCTTGCCCGCACCAAGGTGGGCGAGGAGCTGAAGCTCATCGACACCGAGCGGTTCGA
GCTGGCCTGGATCGTGGACTTCCCCTTCTACGAATGGTCGGACGAGGAGAAGAAGATCGACTTCTCCCACAACCCCTTCT
CCATGCCGCAGGGCGGGCTTGAGGCGCTGAACGGGCAGGACCCGCTCACCATCAAGGCCTTCCAGTACGACATCGCCTGC
AACGGCTACGAGATCGCCTCCGGCGGCATCCGCAACCACCGCCCCGAGGCGATGGTGAAGGCGTTCGAGCTGGCCGGCTA
TGACGAGGCGACCGTCATCGAGCGCTTCGGCGGCATGTACCGCGCCTTCCAGTATGGCGCGCCGCCCCACGGAGGCATGG
CGGCGGGCATCGACCGCATCGTGATGCTCCTGTGCGGCGTCTCGAACCTGCGCGAGATATCGCTCTTCCCCATGAACCAG
CAGGCCCTCGACCTGCTCATGGGCGCGCCGAACGAGGCGACGCCCAAGCAGATGAAGGAACTGCACATCCGTCCGGCCTT
GCCGGCGAAGTGA

Upstream 100 bases:

>100_bases
TTGACAAGGCAGGGTGCGCATGCACCTTCCCCGCCGGCCCGGCGTGAGCCTTTGGCGCCAAACTCCCGTTTCGTTTACCG
TGTTCCCCGAAAGGTCCGCC

Downstream 100 bases:

>100_bases
GGCGAACGGGGGCGGGCATCGGCTGGGTCCCCGGGCGGATTGCTCCTGCCCGAACCGCCGCTGCCCCTGCCAGTCCTCCC
ATCCGAAGGCACATACCAAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MHRYRTHTCGALTEAQVGDTVRVSGWCHRIRDHGGVLFVDLRDHYGLTQVVFDPDSAAFAAAEKVRAEWVIRVDGRVRLR
PEGTENSELATGKVEIYATDLEVLGPAAELPLPVFGDQDYPEDIRLRYRFLDLRRERIHGNIMKRGQIIDSLRQRMKGQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLIMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQDDVFAAVEPVIRGVFEEFGGGKPVTQNWPRIPYAEALRKYGSDKPDLRNPLVMQNVSEHFRGSNFKVFARLLED
PKNEVWAIPGPTGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIREQLGLKDGDAAFFVAGNP
EKFYKFAGLARTKVGEELKLIDTERFELAWIVDFPFYEWSDEEKKIDFSHNPFSMPQGGLEALNGQDPLTIKAFQYDIAC
NGYEIASGGIRNHRPEAMVKAFELAGYDEATVIERFGGMYRAFQYGAPPHGGMAAGIDRIVMLLCGVSNLREISLFPMNQ
QALDLLMGAPNEATPKQMKELHIRPALPAK

Sequences:

>Translated_590_residues
MHRYRTHTCGALTEAQVGDTVRVSGWCHRIRDHGGVLFVDLRDHYGLTQVVFDPDSAAFAAAEKVRAEWVIRVDGRVRLR
PEGTENSELATGKVEIYATDLEVLGPAAELPLPVFGDQDYPEDIRLRYRFLDLRRERIHGNIMKRGQIIDSLRQRMKGQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLIMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQDDVFAAVEPVIRGVFEEFGGGKPVTQNWPRIPYAEALRKYGSDKPDLRNPLVMQNVSEHFRGSNFKVFARLLED
PKNEVWAIPGPTGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIREQLGLKDGDAAFFVAGNP
EKFYKFAGLARTKVGEELKLIDTERFELAWIVDFPFYEWSDEEKKIDFSHNPFSMPQGGLEALNGQDPLTIKAFQYDIAC
NGYEIASGGIRNHRPEAMVKAFELAGYDEATVIERFGGMYRAFQYGAPPHGGMAAGIDRIVMLLCGVSNLREISLFPMNQ
QALDLLMGAPNEATPKQMKELHIRPALPAK
>Mature_590_residues
MHRYRTHTCGALTEAQVGDTVRVSGWCHRIRDHGGVLFVDLRDHYGLTQVVFDPDSAAFAAAEKVRAEWVIRVDGRVRLR
PEGTENSELATGKVEIYATDLEVLGPAAELPLPVFGDQDYPEDIRLRYRFLDLRRERIHGNIMKRGQIIDSLRQRMKGQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLIMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQDDVFAAVEPVIRGVFEEFGGGKPVTQNWPRIPYAEALRKYGSDKPDLRNPLVMQNVSEHFRGSNFKVFARLLED
PKNEVWAIPGPTGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIREQLGLKDGDAAFFVAGNP
EKFYKFAGLARTKVGEELKLIDTERFELAWIVDFPFYEWSDEEKKIDFSHNPFSMPQGGLEALNGQDPLTIKAFQYDIAC
NGYEIASGGIRNHRPEAMVKAFELAGYDEATVIERFGGMYRAFQYGAPPHGGMAAGIDRIVMLLCGVSNLREISLFPMNQ
QALDLLMGAPNEATPKQMKELHIRPALPAK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=603, Percent_Identity=33.4991708126036, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI45439306, Length=328, Percent_Identity=26.219512195122, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226491502, Length=252, Percent_Identity=23.4126984126984, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI4758762, Length=254, Percent_Identity=25.9842519685039, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=41.0562180579216, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=27.891156462585, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789256, Length=220, Percent_Identity=30.9090909090909, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=32.396694214876, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI71984122, Length=295, Percent_Identity=24.0677966101695, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17551876, Length=122, Percent_Identity=37.7049180327869, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=641, Percent_Identity=28.5491419656786, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6321807, Length=245, Percent_Identity=27.7551020408163, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=32.7212020033389, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24640849, Length=272, Percent_Identity=25.7352941176471, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640851, Length=271, Percent_Identity=25.4612546125461, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921528, Length=226, Percent_Identity=25.2212389380531, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_AZOC5 (A8IFX1)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001526575.1
- ProteinModelPortal:   A8IFX1
- SMR:   A8IFX1
- GeneID:   5687877
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3659
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   ACAU438753:AZC_3659-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66325; Mature: 66325

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRTHTCGALTEAQVGDTVRVSGWCHRIRDHGGVLFVDLRDHYGLTQVVFDPDSAAFA
CCCCCCCCCCCCCHHCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
AAEKVRAEWVIRVDGRVRLRPEGTENSELATGKVEIYATDLEVLGPAAELPLPVFGDQDY
HHHHHCEEEEEEECCEEEECCCCCCCCCEEECEEEEEEECHHHHCCHHHCCCCCCCCCCC
PEDIRLRYRFLDLRRERIHGNIMKRGQIIDSLRQRMKGQGFFEFQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLIMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQDDVFAAVEPVIRGVFEEFGGGKPVTQNWPRIPYAEALRKYGSDKPDLRNPLVMQ
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
NVSEHFRGSNFKVFARLLEDPKNEVWAIPGPTGGSRAFCDRMNSWAQGEGQPGLGYIMWR
HHHHHHCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHCHHCCCCCCCCCEEEEEE
EGGEGAGPLANNIGPERTAAIREQLGLKDGDAAFFVAGNPEKFYKFAGLARTKVGEELKL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEE
IDTERFELAWIVDFPFYEWSDEEKKIDFSHNPFSMPQGGLEALNGQDPLTIKAFQYDIAC
EECCCEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCEEEEEEEEEEEE
NGYEIASGGIRNHRPEAMVKAFELAGYDEATVIERFGGMYRAFQYGAPPHGGMAAGIDRI
CCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHH
VMLLCGVSNLREISLFPMNQQALDLLMGAPNEATPKQMKELHIRPALPAK
HHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHEECCCCCCCC
>Mature Secondary Structure
MHRYRTHTCGALTEAQVGDTVRVSGWCHRIRDHGGVLFVDLRDHYGLTQVVFDPDSAAFA
CCCCCCCCCCCCCHHCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
AAEKVRAEWVIRVDGRVRLRPEGTENSELATGKVEIYATDLEVLGPAAELPLPVFGDQDY
HHHHHCEEEEEEECCEEEECCCCCCCCCEEECEEEEEEECHHHHCCHHHCCCCCCCCCCC
PEDIRLRYRFLDLRRERIHGNIMKRGQIIDSLRQRMKGQGFFEFQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLIMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQDDVFAAVEPVIRGVFEEFGGGKPVTQNWPRIPYAEALRKYGSDKPDLRNPLVMQ
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
NVSEHFRGSNFKVFARLLEDPKNEVWAIPGPTGGSRAFCDRMNSWAQGEGQPGLGYIMWR
HHHHHHCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHCHHCCCCCCCCCEEEEEE
EGGEGAGPLANNIGPERTAAIREQLGLKDGDAAFFVAGNPEKFYKFAGLARTKVGEELKL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEE
IDTERFELAWIVDFPFYEWSDEEKKIDFSHNPFSMPQGGLEALNGQDPLTIKAFQYDIAC
EECCCEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCEEEEEEEEEEEE
NGYEIASGGIRNHRPEAMVKAFELAGYDEATVIERFGGMYRAFQYGAPPHGGMAAGIDRI
CCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHH
VMLLCGVSNLREISLFPMNQQALDLLMGAPNEATPKQMKELHIRPALPAK
HHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA