Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 158425272

GI number: 158425272

Start: 4190391

End: 4191812

Strand: Reverse

Name: puuA [H]

Synonym: AZC_3648

Alternate gene names: 158425272

Gene position: 4191812-4190391 (Counterclockwise)

Preceding gene: 158425276

Following gene: 158425271

Centisome position: 78.06

GC content: 67.79

Gene sequence:

>1422_bases
TTGGATGATGGAAAAATCGCATTTTTTTACGGAAGTCACATGCAAGCTGCCGAATGCGACCCCGCCGCGGGCGCCGAAGC
TGCTCCGTCGGGGGACGAAGCGGCCGATTTCATAGCCCGTTTCGGACAGCCGGACATCGTCGAGGTGCTGCTGCCCGATA
CCAGCGGCGTGCTGCGCGGCAAGTGGGTGCCCGGCACCAGCATGGGCAAGATCTGGCGCGACGGCGTGCCGATGCCCCGC
TCACTGTTCGGCCTGGATATCTGGGGCCGGGAAGTCAATTCCACCGGCATCCATATTGAGACCGGCGACCGTGACGGCCT
GTGCCGGCCCATCCCCGGCACGCTGAAGCCGGTGCCCTGGTCACCGCGTCCGGCGGCGCAGGTGCTGCTCACCATGCATC
AGGACGACGGCGCGCCCTGGATGCTGGACCCGCGCCAGCAACTGGTGCGGCAGGTGGAGCGGGCGCGGGCGCTGGGCTTC
GAGCCCACGGTGGCGTTCGAGCTCGAATTCTATCTGCTCAAGCCCTGGGGCCCCGGCGAGGCGCCGGTGCCGATCTTCCC
GCACACGGGCGAAGCCCGGCAGAACATGTATTCCATGTCCGATCTCGATGCCTGCGCCGGCGTGCTGCACGAGATGCGCG
AGGCGGCCAAGGTGCAGGGCCTGCCGGCGGACACGGTGATTTCGGAAGCCGCGCCGGGGCAGTTCGAGATCAATCTCTAT
CACCGCCCCGATGCGCTGGCGGCGGCGGACGAAGCCATCCTGCTGCGGCGCCTCATCGATGGCGTGGCCCGCCGGAACGG
CATGCGGGCCACCTTCATGGCCAAGCCCTTCGCCGAGTTCGCGGGCAATGGCATGCATGTGCACATCAGCCTTGGCGGTG
TGGACGGCACGAATGCCTTCGCCCGGCCCGACGGCGTGGGCGAGCGCCTGTTGCGCTATGCGGCGGCCGGCCTGCTCGCC
ACCATGAAGGACACGCTGCTGTTCTTCGTGCCGAGCTTCAACGGCTATCGACGCCTCGTGCCCGCGAGCTATGCGCCCAC
CGGCATCAGCTGGGGCTATGACAATCGCTCCGTGGCCGTGCGCGTGCCCAACGGACCGCCGGCCGCCCGCCGGCTGGAAC
ACCGCATCGCCGGGGCCGAGGCCCATCCGCATCTGGTCATGGCGGGCATTCTCGCCGGCATGCTGGAAGGCATCGAGCGG
GAAATGGAGCCGCCGCCGCCGCTTAGTGGCAACGCCTTCGAGGGCGAGACGGAGCGGCTGTCGCCCTCCATGGCCGCGGC
GGTGGAGGGCTTCCTCGCTTCCGACTTCATCACCCGCACCTTCGGCGCGGACTACCAGCGCATCTATGGGGTGATGAAGC
AGGAGGAATGCGGGGCCTTCACCCGCGCCATCCTGCCGCTGGAATATGAGACTTATCTGTAG

Upstream 100 bases:

>100_bases
CCATCGCGACTGGCAGCCCCTTGTCCAAGACCAAACTCCCGACACGCATCAACGGAGGCAATAAGCTCCGCTGAAGCTTG
TCGGTTCCATCGAGTCGCGC

Downstream 100 bases:

>100_bases
CTTAGCCGCAAAAAGAGGGGTCCGGTTGTGGCATTCCGGGCACGCCCGGTTCGCCCCGCGCCGTCGTAGCGGTCTACCGC
ATTGACCTAACCGCAGGTGC

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MDDGKIAFFYGSHMQAAECDPAAGAEAAPSGDEAADFIARFGQPDIVEVLLPDTSGVLRGKWVPGTSMGKIWRDGVPMPR
SLFGLDIWGREVNSTGIHIETGDRDGLCRPIPGTLKPVPWSPRPAAQVLLTMHQDDGAPWMLDPRQQLVRQVERARALGF
EPTVAFELEFYLLKPWGPGEAPVPIFPHTGEARQNMYSMSDLDACAGVLHEMREAAKVQGLPADTVISEAAPGQFEINLY
HRPDALAAADEAILLRRLIDGVARRNGMRATFMAKPFAEFAGNGMHVHISLGGVDGTNAFARPDGVGERLLRYAAAGLLA
TMKDTLLFFVPSFNGYRRLVPASYAPTGISWGYDNRSVAVRVPNGPPAARRLEHRIAGAEAHPHLVMAGILAGMLEGIER
EMEPPPPLSGNAFEGETERLSPSMAAAVEGFLASDFITRTFGADYQRIYGVMKQEECGAFTRAILPLEYETYL

Sequences:

>Translated_473_residues
MDDGKIAFFYGSHMQAAECDPAAGAEAAPSGDEAADFIARFGQPDIVEVLLPDTSGVLRGKWVPGTSMGKIWRDGVPMPR
SLFGLDIWGREVNSTGIHIETGDRDGLCRPIPGTLKPVPWSPRPAAQVLLTMHQDDGAPWMLDPRQQLVRQVERARALGF
EPTVAFELEFYLLKPWGPGEAPVPIFPHTGEARQNMYSMSDLDACAGVLHEMREAAKVQGLPADTVISEAAPGQFEINLY
HRPDALAAADEAILLRRLIDGVARRNGMRATFMAKPFAEFAGNGMHVHISLGGVDGTNAFARPDGVGERLLRYAAAGLLA
TMKDTLLFFVPSFNGYRRLVPASYAPTGISWGYDNRSVAVRVPNGPPAARRLEHRIAGAEAHPHLVMAGILAGMLEGIER
EMEPPPPLSGNAFEGETERLSPSMAAAVEGFLASDFITRTFGADYQRIYGVMKQEECGAFTRAILPLEYETYL
>Mature_473_residues
MDDGKIAFFYGSHMQAAECDPAAGAEAAPSGDEAADFIARFGQPDIVEVLLPDTSGVLRGKWVPGTSMGKIWRDGVPMPR
SLFGLDIWGREVNSTGIHIETGDRDGLCRPIPGTLKPVPWSPRPAAQVLLTMHQDDGAPWMLDPRQQLVRQVERARALGF
EPTVAFELEFYLLKPWGPGEAPVPIFPHTGEARQNMYSMSDLDACAGVLHEMREAAKVQGLPADTVISEAAPGQFEINLY
HRPDALAAADEAILLRRLIDGVARRNGMRATFMAKPFAEFAGNGMHVHISLGGVDGTNAFARPDGVGERLLRYAAAGLLA
TMKDTLLFFVPSFNGYRRLVPASYAPTGISWGYDNRSVAVRVPNGPPAARRLEHRIAGAEAHPHLVMAGILAGMLEGIER
EMEPPPPLSGNAFEGETERLSPSMAAAVEGFLASDFITRTFGADYQRIYGVMKQEECGAFTRAILPLEYETYL

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=415, Percent_Identity=23.6144578313253, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI87081870, Length=445, Percent_Identity=38.876404494382, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1790301, Length=284, Percent_Identity=29.5774647887324, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51411; Mature: 51411

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDGKIAFFYGSHMQAAECDPAAGAEAAPSGDEAADFIARFGQPDIVEVLLPDTSGVLRG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCEEE
KWVPGTSMGKIWRDGVPMPRSLFGLDIWGREVNSTGIHIETGDRDGLCRPIPGTLKPVPW
CCCCCCCHHHHHHCCCCCCHHHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
SPRPAAQVLLTMHQDDGAPWMLDPRQQLVRQVERARALGFEPTVAFELEFYLLKPWGPGE
CCCCHHEEEEEEECCCCCCCEECHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCC
APVPIFPHTGEARQNMYSMSDLDACAGVLHEMREAAKVQGLPADTVISEAAPGQFEINLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEE
HRPDALAAADEAILLRRLIDGVARRNGMRATFMAKPFAEFAGNGMHVHISLGGVDGTNAF
ECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHCCCHHHHCCCCEEEEEEECCCCCCCCC
ARPDGVGERLLRYAAAGLLATMKDTLLFFVPSFNGYRRLVPASYAPTGISWGYDNRSVAV
CCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEECCCCCCCCCCCCCCCCCCEEEE
RVPNGPPAARRLEHRIAGAEAHPHLVMAGILAGMLEGIEREMEPPPPLSGNAFEGETERL
ECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHC
SPSMAAAVEGFLASDFITRTFGADYQRIYGVMKQEECGAFTRAILPLEYETYL
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCEEHHCCC
>Mature Secondary Structure
MDDGKIAFFYGSHMQAAECDPAAGAEAAPSGDEAADFIARFGQPDIVEVLLPDTSGVLRG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCEEE
KWVPGTSMGKIWRDGVPMPRSLFGLDIWGREVNSTGIHIETGDRDGLCRPIPGTLKPVPW
CCCCCCCHHHHHHCCCCCCHHHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
SPRPAAQVLLTMHQDDGAPWMLDPRQQLVRQVERARALGFEPTVAFELEFYLLKPWGPGE
CCCCHHEEEEEEECCCCCCCEECHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCC
APVPIFPHTGEARQNMYSMSDLDACAGVLHEMREAAKVQGLPADTVISEAAPGQFEINLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEE
HRPDALAAADEAILLRRLIDGVARRNGMRATFMAKPFAEFAGNGMHVHISLGGVDGTNAF
ECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHCCCHHHHCCCCEEEEEEECCCCCCCCC
ARPDGVGERLLRYAAAGLLATMKDTLLFFVPSFNGYRRLVPASYAPTGISWGYDNRSVAV
CCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEECCCCCCCCCCCCCCCCCCEEEE
RVPNGPPAARRLEHRIAGAEAHPHLVMAGILAGMLEGIEREMEPPPPLSGNAFEGETERL
ECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHC
SPSMAAAVEGFLASDFITRTFGADYQRIYGVMKQEECGAFTRAILPLEYETYL
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]