| Definition | Azorhizobium caulinodans ORS 571, complete genome. |
|---|---|
| Accession | NC_009937 |
| Length | 5,369,772 |
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The map label for this gene is dnaG [H]
Identifier: 158425266
GI number: 158425266
Start: 4179404
End: 4181335
Strand: Direct
Name: dnaG [H]
Synonym: AZC_3642
Alternate gene names: 158425266
Gene position: 4179404-4181335 (Clockwise)
Preceding gene: 158425263
Following gene: 158425267
Centisome position: 77.83
GC content: 68.84
Gene sequence:
>1932_bases ATGCGCTTCCCGCCCTCTTTCCTCGACGAGATCCGAACCCGGCTGCCGGTTTCGGAAGTGGTGGGGCGACGGGTCAAGCT GAAGAAGCAGGGCCGCGAGTTCGCCGGCCTCTCGCCCTTCAATGCGGAAAAGACGCCCTCCTTCTTCGTCAATGACCAGA AGGGGTTCTATCACTGCTTCTCCTCGGGAAAGCACGGCGACGTGTTCGACTTCCTGATGGAGACCGAAGGCCTGCCCTTC GGTGAAGCGGTGGAACGGCTCGCGTCCATGGCCGGCCTGCCGCTGCCGGACGTCACGCCGGAGGCCGCCGCCAAGGAGGC CCGCCGCCGCACGCTGCATGAGGTCATGGAGATGGCCGCCGCCTTCTTCGAGGCGGAACTTCAGGCACGGGCCGGCGCCA AGGCACGGGGCTATCTCTCCGATCGCGGGCTCGGCCCCTCCACGCAGAAGCGCTTCCGCCTCGGCTACGCGCCGGGCGAC CGCTTCGCGCTGAAGGAGGCGCTCGGCAAGGCGGGCGTCCCGGTCTCCGACATGGTCGAGACCGGCCTTCTCATCGCCGG TGACGACATTCCCGTGCCCTACGACAGGTTCCGGGACCGGGTGATGTTCCCCATCACCGATCTGAAGGGCCGCGTCGTGG CCTTCGGGGGCCGGGCGCTGGAGAAGGACGTTCCGGCCAAATACCTGAACTCGCCGGAGACGCCGCTCTTCCACAAGGGC TCTCTGCTCTACAACGCTTATTCCGCCCGTGCCGCCGTCCAGAAGGGCGCACCCGTCATCGCGGTGGAAGGTTATGTGGA CGTGATCGCGCTGGTGGAGGCAGGCTTCGAGGGTGCCGTCGCCCCGCTCGGCACCGCCCTCACCGAGGAGCAGCTCCAGT TGCTCTGGCGTATGGCGGACGAGCCGGTGCTGCTGTTCGACGGCGACAAGGCCGGCCGCCGCGCCGCCTTCCGCGCGGTG GGGCTTGCCTTGCCCCACATCAAGCCGGGCAAGAGCCTCTCCTTCGGCGCGCTGCCGGACGGGCAGGACCCGGACGACCT CATCCGTGCCAGCGGACGCGAGGCCATGGAAGGCGTGCTGGCGCAGGCGCAGCCGCTCTCCGCCATGCTGTGGGCGCGGG AGACCGAGGGCAGCAACCTCGAGACGCCGGAGCGCCGGGCGGCGCTGGAAGCGCGCATCGGCGAGGCGGTGGCCGCCATT CAGGACGAGAACGTCCGCCGCCATTACAAGCAGGATCTGCAGGATCGCCTGCGCCGCCTGCTCTCCCCCGGCAGCGAGAA CCGGCCGCGCCGATTCGAGGGCGGCTGGAGCGCTGCGCCCGGTCGCTCGGGCAATTTCCGGCCCCAGAACGGCCGTTTCA TGCGCGAGCCGCCGCTGGTGCCGGGCACGGAGCTGGCACGATCTGCGGTGGTGCGGGGGCCTCTCGCCAGCCTGCCGCGC AACGAGGCGCTGCTGCTCTTCTGCCTCATCAACCATCCCTGGCTGATGGACGAATATGCCGAGGAGGTCGCCGGCCTGCC CTTTGCCAACAAGGACGCGGACCGGCTCCGGCGCGCGCTTCTGGAGGCGCATCTGGAAGGCGCCCATGAGGGCGAGGCGC TCACCGAACATCTGACGCGCGAGGATCTCGACGCCCTCGCGCAGCGCGTGCGGGCCTTCGCCAATCCGAAGATGGACTGG CCGGCCTATCCCGACACCGCTCCGGAGGACGCAAGGCTCTGGTGGTTGCAGCGCGCGGCCTTGCATCAAAAGGCTTACGC GCTATCTAAGGAACTGAGGGAAGCTGAACGTTCCCTGGGCTCCGATCCGAGCGAGGCGAACTTCGCGTGGCTGCGCGATG TGCGCGAGCGCCTTTCGGCAGCCGAGGGAACGGAGGCCCTGGTGGAAGGTTTCGGCGCCTCGTCCGGCCGCGGATCGCAC AAATCCGTCTAG
Upstream 100 bases:
>100_bases AAAACCTCCATCGTGACCGGCCCCAACGGGCTGTTCGGATGCCACGATGTATATACAAACGTAATCTGTCAAGAATCCCG CACCCGTGCTAAATAGGCCC
Downstream 100 bases:
>100_bases CCGCTCTGCGCTTTTTGAAGGGCCAAGGGGGCCTGCCGCCTCTTTTTGGGGTTGGAGCGGCCTTCAGGCTGGTTTAACGG GATCGCCATAGGGTGGCCCG
Product: DNA primase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 643; Mature: 643
Protein sequence:
>643_residues MRFPPSFLDEIRTRLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCFSSGKHGDVFDFLMETEGLPF GEAVERLASMAGLPLPDVTPEAAAKEARRRTLHEVMEMAAAFFEAELQARAGAKARGYLSDRGLGPSTQKRFRLGYAPGD RFALKEALGKAGVPVSDMVETGLLIAGDDIPVPYDRFRDRVMFPITDLKGRVVAFGGRALEKDVPAKYLNSPETPLFHKG SLLYNAYSARAAVQKGAPVIAVEGYVDVIALVEAGFEGAVAPLGTALTEEQLQLLWRMADEPVLLFDGDKAGRRAAFRAV GLALPHIKPGKSLSFGALPDGQDPDDLIRASGREAMEGVLAQAQPLSAMLWARETEGSNLETPERRAALEARIGEAVAAI QDENVRRHYKQDLQDRLRRLLSPGSENRPRRFEGGWSAAPGRSGNFRPQNGRFMREPPLVPGTELARSAVVRGPLASLPR NEALLLFCLINHPWLMDEYAEEVAGLPFANKDADRLRRALLEAHLEGAHEGEALTEHLTREDLDALAQRVRAFANPKMDW PAYPDTAPEDARLWWLQRAALHQKAYALSKELREAERSLGSDPSEANFAWLRDVRERLSAAEGTEALVEGFGASSGRGSH KSV
Sequences:
>Translated_643_residues MRFPPSFLDEIRTRLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCFSSGKHGDVFDFLMETEGLPF GEAVERLASMAGLPLPDVTPEAAAKEARRRTLHEVMEMAAAFFEAELQARAGAKARGYLSDRGLGPSTQKRFRLGYAPGD RFALKEALGKAGVPVSDMVETGLLIAGDDIPVPYDRFRDRVMFPITDLKGRVVAFGGRALEKDVPAKYLNSPETPLFHKG SLLYNAYSARAAVQKGAPVIAVEGYVDVIALVEAGFEGAVAPLGTALTEEQLQLLWRMADEPVLLFDGDKAGRRAAFRAV GLALPHIKPGKSLSFGALPDGQDPDDLIRASGREAMEGVLAQAQPLSAMLWARETEGSNLETPERRAALEARIGEAVAAI QDENVRRHYKQDLQDRLRRLLSPGSENRPRRFEGGWSAAPGRSGNFRPQNGRFMREPPLVPGTELARSAVVRGPLASLPR NEALLLFCLINHPWLMDEYAEEVAGLPFANKDADRLRRALLEAHLEGAHEGEALTEHLTREDLDALAQRVRAFANPKMDW PAYPDTAPEDARLWWLQRAALHQKAYALSKELREAERSLGSDPSEANFAWLRDVRERLSAAEGTEALVEGFGASSGRGSH KSV >Mature_643_residues MRFPPSFLDEIRTRLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCFSSGKHGDVFDFLMETEGLPF GEAVERLASMAGLPLPDVTPEAAAKEARRRTLHEVMEMAAAFFEAELQARAGAKARGYLSDRGLGPSTQKRFRLGYAPGD RFALKEALGKAGVPVSDMVETGLLIAGDDIPVPYDRFRDRVMFPITDLKGRVVAFGGRALEKDVPAKYLNSPETPLFHKG SLLYNAYSARAAVQKGAPVIAVEGYVDVIALVEAGFEGAVAPLGTALTEEQLQLLWRMADEPVLLFDGDKAGRRAAFRAV GLALPHIKPGKSLSFGALPDGQDPDDLIRASGREAMEGVLAQAQPLSAMLWARETEGSNLETPERRAALEARIGEAVAAI QDENVRRHYKQDLQDRLRRLLSPGSENRPRRFEGGWSAAPGRSGNFRPQNGRFMREPPLVPGTELARSAVVRGPLASLPR NEALLLFCLINHPWLMDEYAEEVAGLPFANKDADRLRRALLEAHLEGAHEGEALTEHLTREDLDALAQRVRAFANPKMDW PAYPDTAPEDARLWWLQRAALHQKAYALSKELREAERSLGSDPSEANFAWLRDVRERLSAAEGTEALVEGFGASSGRGSH KSV
Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]
COG id: COG0358
COG function: function code L; DNA primase (bacterial type)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Escherichia coli, GI1789447, Length=418, Percent_Identity=41.866028708134, Blast_Score=331, Evalue=1e-91,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016136 - InterPro: IPR013264 - InterPro: IPR019475 - InterPro: IPR006295 - InterPro: IPR013173 - InterPro: IPR006171 - InterPro: IPR002694 [H]
Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]
EC number: 2.7.7.-
Molecular weight: Translated: 70717; Mature: 70717
Theoretical pI: Translated: 6.67; Mature: 6.67
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRFPPSFLDEIRTRLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCF CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEECCCCCHHHHH SSGKHGDVFDFLMETEGLPFGEAVERLASMAGLPLPDVTPEAAAKEARRRTLHEVMEMAA CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH AFFEAELQARAGAKARGYLSDRGLGPSTQKRFRLGYAPGDRFALKEALGKAGVPVSDMVE HHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHEEECCCCCHHHHHHHHHCCCCCCHHHHHH TGLLIAGDDIPVPYDRFRDRVMFPITDLKGRVVAFGGRALEKDVPAKYLNSPETPLFHKG CCCEEECCCCCCCHHHHCCCEEEEEECCCCCEEEECCCHHHHCCCHHHCCCCCCCCEECC SLLYNAYSARAAVQKGAPVIAVEGYVDVIALVEAGFEGAVAPLGTALTEEQLQLLWRMAD CHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC EPVLLFDGDKAGRRAAFRAVGLALPHIKPGKSLSFGALPDGQDPDDLIRASGREAMEGVL CCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHH AQAQPLSAMLWARETEGSNLETPERRAALEARIGEAVAAIQDENVRRHYKQDLQDRLRRL HHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LSPGSENRPRRFEGGWSAAPGRSGNFRPQNGRFMREPPLVPGTELARSAVVRGPLASLPR HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCC NEALLLFCLINHPWLMDEYAEEVAGLPFANKDADRLRRALLEAHLEGAHEGEALTEHLTR CCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH EDLDALAQRVRAFANPKMDWPAYPDTAPEDARLWWLQRAALHQKAYALSKELREAERSLG HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC SDPSEANFAWLRDVRERLSAAEGTEALVEGFGASSGRGSHKSV CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC >Mature Secondary Structure MRFPPSFLDEIRTRLPVSEVVGRRVKLKKQGREFAGLSPFNAEKTPSFFVNDQKGFYHCF CCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEECCCCCHHHHH SSGKHGDVFDFLMETEGLPFGEAVERLASMAGLPLPDVTPEAAAKEARRRTLHEVMEMAA CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH AFFEAELQARAGAKARGYLSDRGLGPSTQKRFRLGYAPGDRFALKEALGKAGVPVSDMVE HHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHEEECCCCCHHHHHHHHHCCCCCCHHHHHH TGLLIAGDDIPVPYDRFRDRVMFPITDLKGRVVAFGGRALEKDVPAKYLNSPETPLFHKG CCCEEECCCCCCCHHHHCCCEEEEEECCCCCEEEECCCHHHHCCCHHHCCCCCCCCEECC SLLYNAYSARAAVQKGAPVIAVEGYVDVIALVEAGFEGAVAPLGTALTEEQLQLLWRMAD CHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC EPVLLFDGDKAGRRAAFRAVGLALPHIKPGKSLSFGALPDGQDPDDLIRASGREAMEGVL CCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHH AQAQPLSAMLWARETEGSNLETPERRAALEARIGEAVAAIQDENVRRHYKQDLQDRLRRL HHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LSPGSENRPRRFEGGWSAAPGRSGNFRPQNGRFMREPPLVPGTELARSAVVRGPLASLPR HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCC NEALLLFCLINHPWLMDEYAEEVAGLPFANKDADRLRRALLEAHLEGAHEGEALTEHLTR CCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH EDLDALAQRVRAFANPKMDWPAYPDTAPEDARLWWLQRAALHQKAYALSKELREAERSLG HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC SDPSEANFAWLRDVRERLSAAEGTEALVEGFGASSGRGSHKSV CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]