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The map label for this gene is carA [H]

Identifier: 158425262

GI number: 158425262

Start: 4176776

End: 4177981

Strand: Reverse

Name: carA [H]

Synonym: AZC_3638

Alternate gene names: 158425262

Gene position: 4177981-4176776 (Counterclockwise)

Preceding gene: 158425264

Following gene: 158425260

Centisome position: 77.81

GC content: 66.92

Gene sequence:

>1206_bases
ATGACGCAGGAACAAGACGCACCCGGCTCCGCCGAGGGCTGGACCGAACCGAAGCCGACCGCCCTTCTGGTTCTCGCTGA
TGGCACCGTGCTGGAAGGCTTCGGCTTCGGCGCGGCCACCGAGGGCGTAGGGGAGGTGTGCTTCAACACCGCCATCACCG
GCTATGAGGAGATTCTCACCGATCCCTCCTATGCCGGGCAGATCATCACCTTCACCTTCCCGCACATTGGCAATGTGGGC
ACGAATGAGGAGGACATCGAGACGGTGTCCGCCGCCGCCGTGTCCGGCGTGCGCGGGGTGGTGGTGCGCGGATCGGTGAC
CGATCCGTCCAACTACCGGGCCACCCGCCATCTCGACGCCTGGCTGAAGGCGCGGGACATTCCCGCCATCAGCGGCATCG
ACACCCGCGCGCTCACCGCCCTCATCCGCGAAGGCGGCATGCCGAATGCGGTGCTCGCCCACAATCCGGCCGGCGTGTTC
GACATCGAGGCGCTCAAGGCCAAGGCCAAGGAATGGCCGGGCCTCGTCGGCATGGACCTCGTGCCGGGCGTCACCTCGGT
CCAGCGCTTCAACTGGGACGAGACCGAGTGGCAGTGGAATGGCGGCTATGGCCACCAGGGCGCGCCCAAGTATCACGTGG
TCGCCATCGACTATGGCGTGAAGCGCAACATCCTGCGCCTGCTCGCCACCGCCGGCTGCAAGGTGACCGTGGTTCCCGCC
ACCACCTCGGCGGCGGATGTGCTGGCGCTCCAGCCGGACGGCGTGTTCCTGTCCAACGGGCCCGGTGATCCGGCGGCGAC
AGGCGAATATGCGGTGCCGGTGATCCGCGAGCTGATCGCCAAGGAGATCCCCACGTTCGGCATCTGCCTCGGCCACCAGA
TGATCGGCCTCGCGGTCGGTGGCCGCACCCTGAAGATGCACCAGGGCCACCACGGCGCGAACCATCCGGTGAAGGATCTG
ACCACCGGCAAGGTGGAGATCACCTCCATGAACCACGGCTTTGCCGTGGATCGCGACAGCCTGCCGGCCAATGCGCAGGA
GACCCATGTCTCCCTGTTCGACGGCTCCAACGCCGGCATCGCGCTGGCCGACAAGCCGGTGTTCTCGGTGCAGTATCACC
CCGAGGCGAGCCCCGGCCCGCGCGACAGCCACTATCTCTTCACGCGTTTCGTGGAACTGATGGCCAAGCACAAGACCGCC
GCCTGA

Upstream 100 bases:

>100_bases
CCCTTGTCACCAGCGGCATCCTCAGGTAGCCGGGCATTCGGCGCACAAATTCGCGCGCATGGCTGGCAACGCCGCGCGCA
GTCCCTTATCTAGGCGATCC

Downstream 100 bases:

>100_bases
GGCATCCGAAGGTCCGAACATAAGAAAGCGCCCGGCCAGACGGTCCGGGCGCTTTTCTTTTGGGACCTCACTCTGCAGGT
TGCCCGCTAGGGCGGCTGGT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTQEQDAPGSAEGWTEPKPTALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILTDPSYAGQIITFTFPHIGNVG
TNEEDIETVSAAAVSGVRGVVVRGSVTDPSNYRATRHLDAWLKARDIPAISGIDTRALTALIREGGMPNAVLAHNPAGVF
DIEALKAKAKEWPGLVGMDLVPGVTSVQRFNWDETEWQWNGGYGHQGAPKYHVVAIDYGVKRNILRLLATAGCKVTVVPA
TTSAADVLALQPDGVFLSNGPGDPAATGEYAVPVIRELIAKEIPTFGICLGHQMIGLAVGGRTLKMHQGHHGANHPVKDL
TTGKVEITSMNHGFAVDRDSLPANAQETHVSLFDGSNAGIALADKPVFSVQYHPEASPGPRDSHYLFTRFVELMAKHKTA
A

Sequences:

>Translated_401_residues
MTQEQDAPGSAEGWTEPKPTALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILTDPSYAGQIITFTFPHIGNVG
TNEEDIETVSAAAVSGVRGVVVRGSVTDPSNYRATRHLDAWLKARDIPAISGIDTRALTALIREGGMPNAVLAHNPAGVF
DIEALKAKAKEWPGLVGMDLVPGVTSVQRFNWDETEWQWNGGYGHQGAPKYHVVAIDYGVKRNILRLLATAGCKVTVVPA
TTSAADVLALQPDGVFLSNGPGDPAATGEYAVPVIRELIAKEIPTFGICLGHQMIGLAVGGRTLKMHQGHHGANHPVKDL
TTGKVEITSMNHGFAVDRDSLPANAQETHVSLFDGSNAGIALADKPVFSVQYHPEASPGPRDSHYLFTRFVELMAKHKTA
A
>Mature_400_residues
TQEQDAPGSAEGWTEPKPTALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILTDPSYAGQIITFTFPHIGNVGT
NEEDIETVSAAAVSGVRGVVVRGSVTDPSNYRATRHLDAWLKARDIPAISGIDTRALTALIREGGMPNAVLAHNPAGVFD
IEALKAKAKEWPGLVGMDLVPGVTSVQRFNWDETEWQWNGGYGHQGAPKYHVVAIDYGVKRNILRLLATAGCKVTVVPAT
TSAADVLALQPDGVFLSNGPGDPAATGEYAVPVIRELIAKEIPTFGICLGHQMIGLAVGGRTLKMHQGHHGANHPVKDLT
TGKVEITSMNHGFAVDRDSLPANAQETHVSLFDGSNAGIALADKPVFSVQYHPEASPGPRDSHYLFTRFVELMAKHKTAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=378, Percent_Identity=40.7407407407407, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=35.4005167958656, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=35.4005167958656, Blast_Score=214, Evalue=9e-56,
Organism=Escherichia coli, GI1786215, Length=387, Percent_Identity=51.1627906976744, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI193204318, Length=398, Percent_Identity=37.1859296482412, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=36.0406091370558, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=392, Percent_Identity=36.9897959183673, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=38.0829015544041, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=38.0829015544041, Blast_Score=217, Evalue=9e-57,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42518; Mature: 42387

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEQDAPGSAEGWTEPKPTALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILT
CCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCHHHHHC
DPSYAGQIITFTFPHIGNVGTNEEDIETVSAAAVSGVRGVVVRGSVTDPSNYRATRHLDA
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
WLKARDIPAISGIDTRALTALIREGGMPNAVLAHNPAGVFDIEALKAKAKEWPGLVGMDL
HHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHCCCEECCHH
VPGVTSVQRFNWDETEWQWNGGYGHQGAPKYHVVAIDYGVKRNILRLLATAGCKVTVVPA
CCCCHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEC
TTSAADVLALQPDGVFLSNGPGDPAATGEYAVPVIRELIAKEIPTFGICLGHQMIGLAVG
CCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEEEC
GRTLKMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDRDSLPANAQETHVSLFDGSNAGI
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEECCCCCE
ALADKPVFSVQYHPEASPGPRDSHYLFTRFVELMAKHKTAA
EEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQEQDAPGSAEGWTEPKPTALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILT
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCHHHHHC
DPSYAGQIITFTFPHIGNVGTNEEDIETVSAAAVSGVRGVVVRGSVTDPSNYRATRHLDA
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
WLKARDIPAISGIDTRALTALIREGGMPNAVLAHNPAGVFDIEALKAKAKEWPGLVGMDL
HHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHCCCEECCHH
VPGVTSVQRFNWDETEWQWNGGYGHQGAPKYHVVAIDYGVKRNILRLLATAGCKVTVVPA
CCCCHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEC
TTSAADVLALQPDGVFLSNGPGDPAATGEYAVPVIRELIAKEIPTFGICLGHQMIGLAVG
CCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEEEC
GRTLKMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDRDSLPANAQETHVSLFDGSNAGI
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEECCCCCE
ALADKPVFSVQYHPEASPGPRDSHYLFTRFVELMAKHKTAA
EEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]