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The map label for this gene is nifS1 [H]

Identifier: 158425240

GI number: 158425240

Start: 4145508

End: 4146725

Strand: Reverse

Name: nifS1 [H]

Synonym: AZC_3616

Alternate gene names: 158425240

Gene position: 4146725-4145508 (Counterclockwise)

Preceding gene: 158425242

Following gene: 158425239

Centisome position: 77.22

GC content: 72.0

Gene sequence:

>1218_bases
TTGCAAGCCTTTCCGCTTCACCCACGCGGGAGCGCGGTGATGGCGGAGGCGCGCGTCCATCTCGATCACAATGCCACATC
CCCGCTCCGGCCGGAAGCGCGAGCCGCGCTGATGGCCGCGTTGGATCAGGGTGGAAACCCCTCGTCCGTGCATGCGGACG
GGCGCGCCGCGCGCGGGCGCATGGAAGGGGCGCGGGCGGGCGTTGCGGCCCTGTGTGGCGCCCCGGCACGGGGTGTCATG
TTCACCTCCGGCGCCACCGAGGCGAATGCGCTGGCGCTCCATCCCTCCTGGGAGATCGCCGGCCGACCGGTGACCTGCGA
CGTGCTGCTGATGAGCGCCGTCGAGCATCCCGCTGTGCTTCGGGGCCATCGCTTTCCGGACGCGGCCGTCGAGCTGCTGC
CGGTGGATGAGGCCGGGCGGCTGGATCTCGATGCGCTCTCCGATCGGCTCGCGGCCCATGCGGCGGCCGGGCGGCGGGCC
CTGGTGAGCTTGATGGCCGCCAACAACGAGACCGGCGTCATCCAGCCCGTGGCCGAAGCGGCGGAACTCGCCAAGGTTCA
CGGCGGGTTGATGCATTGCGATGCCGTGCAGGCGGCCGGCCGCCTGCCGCTCGACATGACGGCGCTGGGCCTTGATCTCC
TCTCGCTCTCCGCCCACAAGCTCGGCGGGCCGCAGGGCGCCGGTGCGCTGGTGGCGGCGAGCGAGGATACCCGGCCGCTG
GTGCCGCTGCTGCGGGGCGGCGGCCAGGAGCGGGGCCGCCGCGGCGGGACGGAGAACGTGCCGGCGGTCGCGGGCTTCGG
CGCGGCGGCGGAGGCGGCGCGTCTCGCGCAGCCGCAGGAAGCCATGCGCCTCGCGCACTTGCGCGACCTGCTGGAACAGG
AGATTGTCGCCGCCCTTCCGGAAACGGAGGTTGTCGGCGGTGCCGCGGCACGGGTGCCGAACACGAGTTGCATCGCCTTC
GGCGGTTTGAAGGCGGAAACACTGGTGATCGCCCTCGACCTTTCCCACATAGCGGTCAGTGCCGGCTCGGCCTGTTCCTC
GGGGAAGGTGGGGCCCTCGCATGTGTTGTCGGCCATGGGGTTCGATGCCGTGCGCGCCGGCGGAGCGATCCGCCTGTCGC
TGGGCTGGTCGAGCACCGAACAGGATGTGGAACGGGCGATTGCGGCCTTGCGCCGCGTCGTTCCGCAGGTGAGAAGCCGC
TCGCTGAGAGCGGCGTGA

Upstream 100 bases:

>100_bases
CTATTTAGAATAATTCTAATGTATCCCTGCTGACTGCGCGGGGACGCGCTGCACGACAGAGACGGGTGAAGTTGGATCAT
ATGGACGAAAGAAGGCGCAA

Downstream 100 bases:

>100_bases
GGAACTTGACCCGCGGGCCTTGACCCCGCGCGGGAGAGGAGACGGAAGATGCCAGCAGTCCAGGAGACGGTCGAACGCGT
CCGGTCGATCGACGTCGATC

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MQAFPLHPRGSAVMAEARVHLDHNATSPLRPEARAALMAALDQGGNPSSVHADGRAARGRMEGARAGVAALCGAPARGVM
FTSGATEANALALHPSWEIAGRPVTCDVLLMSAVEHPAVLRGHRFPDAAVELLPVDEAGRLDLDALSDRLAAHAAAGRRA
LVSLMAANNETGVIQPVAEAAELAKVHGGLMHCDAVQAAGRLPLDMTALGLDLLSLSAHKLGGPQGAGALVAASEDTRPL
VPLLRGGGQERGRRGGTENVPAVAGFGAAAEAARLAQPQEAMRLAHLRDLLEQEIVAALPETEVVGGAAARVPNTSCIAF
GGLKAETLVIALDLSHIAVSAGSACSSGKVGPSHVLSAMGFDAVRAGGAIRLSLGWSSTEQDVERAIAALRRVVPQVRSR
SLRAA

Sequences:

>Translated_405_residues
MQAFPLHPRGSAVMAEARVHLDHNATSPLRPEARAALMAALDQGGNPSSVHADGRAARGRMEGARAGVAALCGAPARGVM
FTSGATEANALALHPSWEIAGRPVTCDVLLMSAVEHPAVLRGHRFPDAAVELLPVDEAGRLDLDALSDRLAAHAAAGRRA
LVSLMAANNETGVIQPVAEAAELAKVHGGLMHCDAVQAAGRLPLDMTALGLDLLSLSAHKLGGPQGAGALVAASEDTRPL
VPLLRGGGQERGRRGGTENVPAVAGFGAAAEAARLAQPQEAMRLAHLRDLLEQEIVAALPETEVVGGAAARVPNTSCIAF
GGLKAETLVIALDLSHIAVSAGSACSSGKVGPSHVLSAMGFDAVRAGGAIRLSLGWSSTEQDVERAIAALRRVVPQVRSR
SLRAA
>Mature_405_residues
MQAFPLHPRGSAVMAEARVHLDHNATSPLRPEARAALMAALDQGGNPSSVHADGRAARGRMEGARAGVAALCGAPARGVM
FTSGATEANALALHPSWEIAGRPVTCDVLLMSAVEHPAVLRGHRFPDAAVELLPVDEAGRLDLDALSDRLAAHAAAGRRA
LVSLMAANNETGVIQPVAEAAELAKVHGGLMHCDAVQAAGRLPLDMTALGLDLLSLSAHKLGGPQGAGALVAASEDTRPL
VPLLRGGGQERGRRGGTENVPAVAGFGAAAEAARLAQPQEAMRLAHLRDLLEQEIVAALPETEVVGGAAARVPNTSCIAF
GGLKAETLVIALDLSHIAVSAGSACSSGKVGPSHVLSAMGFDAVRAGGAIRLSLGWSSTEQDVERAIAALRRVVPQVRSR
SLRAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=388, Percent_Identity=35.8247422680412, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI156713448, Length=431, Percent_Identity=33.6426914153132, Blast_Score=183, Evalue=2e-46,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=34.3511450381679, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1789175, Length=407, Percent_Identity=27.5184275184275, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787970, Length=369, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=394, Percent_Identity=36.5482233502538, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17533177, Length=322, Percent_Identity=29.5031055900621, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=36.4102564102564, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI20129463, Length=388, Percent_Identity=35.5670103092784, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41399; Mature: 41399

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFPLHPRGSAVMAEARVHLDHNATSPLRPEARAALMAALDQGGNPSSVHADGRAARGR
CCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCH
MEGARAGVAALCGAPARGVMFTSGATEANALALHPSWEIAGRPVTCDVLLMSAVEHPAVL
HHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCHHH
RGHRFPDAAVELLPVDEAGRLDLDALSDRLAAHAAAGRRALVSLMAANNETGVIQPVAEA
CCCCCCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AELAKVHGGLMHCDAVQAAGRLPLDMTALGLDLLSLSAHKLGGPQGAGALVAASEDTRPL
HHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
VPLLRGGGQERGRRGGTENVPAVAGFGAAAEAARLAQPQEAMRLAHLRDLLEQEIVAALP
HHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
ETEVVGGAAARVPNTSCIAFGGLKAETLVIALDLSHIAVSAGSACSSGKVGPSHVLSAMG
CCHHCCCCHHCCCCCCEEEECCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHC
FDAVRAGGAIRLSLGWSSTEQDVERAIAALRRVVPQVRSRSLRAA
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQAFPLHPRGSAVMAEARVHLDHNATSPLRPEARAALMAALDQGGNPSSVHADGRAARGR
CCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCH
MEGARAGVAALCGAPARGVMFTSGATEANALALHPSWEIAGRPVTCDVLLMSAVEHPAVL
HHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCHHH
RGHRFPDAAVELLPVDEAGRLDLDALSDRLAAHAAAGRRALVSLMAANNETGVIQPVAEA
CCCCCCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AELAKVHGGLMHCDAVQAAGRLPLDMTALGLDLLSLSAHKLGGPQGAGALVAASEDTRPL
HHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
VPLLRGGGQERGRRGGTENVPAVAGFGAAAEAARLAQPQEAMRLAHLRDLLEQEIVAALP
HHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
ETEVVGGAAARVPNTSCIAFGGLKAETLVIALDLSHIAVSAGSACSSGKVGPSHVLSAMG
CCHHCCCCHHCCCCCCEEEECCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHC
FDAVRAGGAIRLSLGWSSTEQDVERAIAALRRVVPQVRSRSLRAA
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]