Click here to switch to the map view.

The map label for this gene is thiO/thiG [H]

Identifier: 158425178

GI number: 158425178

Start: 4081706

End: 4083067

Strand: Reverse

Name: thiO/thiG [H]

Synonym: AZC_3554

Alternate gene names: 158425178

Gene position: 4083067-4081706 (Counterclockwise)

Preceding gene: 158425179

Following gene: 158425177

Centisome position: 76.04

GC content: 70.34

Gene sequence:

>1362_bases
GTGGCACTGCCTCCCCCGCGCCGAGATGGCGCAATGGTTTTCCTTGCCGCCGAAGCATCGTGCGGCGATAATGACTTGAC
GGCCTGGGGTGCCGCGCGTCACGCGCGGCTGAGATCACACCCATCGAACCTGTCTGGGTCATGCCAGCGTAGGGAGGAGC
CGATGAGCGAGAGCTTGTCGCATCACGATTCCGCATCTTCGTCCGCCCATTCCGCGCGCGCATCCGGTGCGCGGCCGCGT
GTCGCCATCGTCGGCGGCGGCGTCATGGGCCTGTCCATCGCCTGGCGGCTCGCGGAAGCCGGCTGCACGGTGGAGATCTT
CGAGCAGGGCCGCACGGGCCTTGGCGCCAGCCATGCGGCGGCCGGCATGCTTGCCGCCTGCGCCGAGGCGGAACCCGGCG
AGGAGGGCCTGCTCGCCCTCAATCGCGAGAGCCAGCGCCTGTGGCCCTCCTTTGCCGCCGATCTGGAGGCCGCCTCCGGC
ATGGCGGTGGACCTGCGCACGGACGGCACCATCGTTCTCGCCCTCAATGCGGACGATGCGGCCAAGCTCCGCCACCTCAT
CACCTTCCAGCAGGGCCTCGGCCTGCCGGTGGAGTGGCTGAACGGAGCGGAGGTGCGCCGGCGCGAACCCCATCTCGCCA
CCAAGCTCGCGGGCGCCGTATGGTGCCCCGAGGACCATCAGGTGGACAATCGCAAGGTCTCCGCTGCCTTGCGCGTTGCG
GCGCTGAAGGCCGGCGCGACGTTGCATGAGGACATGCCCGTCGAGCGCGTGGAGGTCGTGGACGGTCGCGCCCGTGCCGT
CATCGCGAAGGGCGAGCGCTTCGGGGCGGATATCGTGGTTCTGGCGGCAGGCGCATGGTCGCGCGGCGTTGCCATTACTC
CCGCGACACCGCTGCCGGTGCGGCCGGTGAAGGGGCAGATGCTGGCGCTCGCCATGGACCCGGCCGCACCCATCCTGAGC
CATGTGGTGTGGGCGCCGGGCACGTATCTCGTACCGCGCAAGGACGGGCGGCTCATCGTTGGTGCGACGGTGGAGGAGCG
CGGCTTCAATGCCGATCTCACCGCCGGCGGGCAGCTGGCGCTGCTCACCCATGCCTGGCGGGCGCTGCCCACCATCGAGG
AACTGCCGATCCTCGAGCAATGGGTCGGCTTCCGCCCCGGCAGCCGGGACGACGCGCCCATTCTCGGCCGCAGCGCCGAG
GTGGAGGGGCTGGTGTTCGCCACCGGCCATCACCGCAACGGCATCCTGCTGCTGCCGGTGACGGCGGAAGCGATCTCCCG
CCTCATCCTTGAGGGTGAGACGGACCCGGTGATCGCGCCCTTCGTGGCGGAACGCTTCCTGCCGCGCGCGGCAGCGGAAT
GA

Upstream 100 bases:

>100_bases
CGTCCAGCTCACGGAATTGCGCGATCGCCTGAACGCCGCCGTCGACTAGGCGGCAACGCCGCCTGCGGTCGGCCCGCCGC
ATTGCGACGGCGGGCGAAAC

Downstream 100 bases:

>100_bases
ACCCGGCCGCGTGACGAACGAAAACGTCCGTGGCAGCGTTGGACCTGAAAGCACAGACAGACGGCGAGAGGCGAGGGACG
TGAAGCAACTGCGCGTGAAC

Product: glycine oxidase precursor

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MALPPPRRDGAMVFLAAEASCGDNDLTAWGAARHARLRSHPSNLSGSCQRREEPMSESLSHHDSASSSAHSARASGARPR
VAIVGGGVMGLSIAWRLAEAGCTVEIFEQGRTGLGASHAAAGMLAACAEAEPGEEGLLALNRESQRLWPSFAADLEAASG
MAVDLRTDGTIVLALNADDAAKLRHLITFQQGLGLPVEWLNGAEVRRREPHLATKLAGAVWCPEDHQVDNRKVSAALRVA
ALKAGATLHEDMPVERVEVVDGRARAVIAKGERFGADIVVLAAGAWSRGVAITPATPLPVRPVKGQMLALAMDPAAPILS
HVVWAPGTYLVPRKDGRLIVGATVEERGFNADLTAGGQLALLTHAWRALPTIEELPILEQWVGFRPGSRDDAPILGRSAE
VEGLVFATGHHRNGILLLPVTAEAISRLILEGETDPVIAPFVAERFLPRAAAE

Sequences:

>Translated_453_residues
MALPPPRRDGAMVFLAAEASCGDNDLTAWGAARHARLRSHPSNLSGSCQRREEPMSESLSHHDSASSSAHSARASGARPR
VAIVGGGVMGLSIAWRLAEAGCTVEIFEQGRTGLGASHAAAGMLAACAEAEPGEEGLLALNRESQRLWPSFAADLEAASG
MAVDLRTDGTIVLALNADDAAKLRHLITFQQGLGLPVEWLNGAEVRRREPHLATKLAGAVWCPEDHQVDNRKVSAALRVA
ALKAGATLHEDMPVERVEVVDGRARAVIAKGERFGADIVVLAAGAWSRGVAITPATPLPVRPVKGQMLALAMDPAAPILS
HVVWAPGTYLVPRKDGRLIVGATVEERGFNADLTAGGQLALLTHAWRALPTIEELPILEQWVGFRPGSRDDAPILGRSAE
VEGLVFATGHHRNGILLLPVTAEAISRLILEGETDPVIAPFVAERFLPRAAAE
>Mature_452_residues
ALPPPRRDGAMVFLAAEASCGDNDLTAWGAARHARLRSHPSNLSGSCQRREEPMSESLSHHDSASSSAHSARASGARPRV
AIVGGGVMGLSIAWRLAEAGCTVEIFEQGRTGLGASHAAAGMLAACAEAEPGEEGLLALNRESQRLWPSFAADLEAASGM
AVDLRTDGTIVLALNADDAAKLRHLITFQQGLGLPVEWLNGAEVRRREPHLATKLAGAVWCPEDHQVDNRKVSAALRVAA
LKAGATLHEDMPVERVEVVDGRARAVIAKGERFGADIVVLAAGAWSRGVAITPATPLPVRPVKGQMLALAMDPAAPILSH
VVWAPGTYLVPRKDGRLIVGATVEERGFNADLTAGGQLALLTHAWRALPTIEELPILEQWVGFRPGSRDDAPILGRSAEV
EGLVFATGHHRNGILLLPVTAEAISRLILEGETDPVIAPFVAERFLPRAAAE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Homo sapiens, GI194306651, Length=373, Percent_Identity=24.3967828418231, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787438, Length=401, Percent_Identity=24.6882793017456, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20130091, Length=382, Percent_Identity=22.7748691099476, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 47873; Mature: 47742

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPPPRRDGAMVFLAAEASCGDNDLTAWGAARHARLRSHPSNLSGSCQRREEPMSESLS
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHH
HHDSASSSAHSARASGARPRVAIVGGGVMGLSIAWRLAEAGCTVEIFEQGRTGLGASHAA
HCCCCCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
AGMLAACAEAEPGEEGLLALNRESQRLWPSFAADLEAASGMAVDLRTDGTIVLALNADDA
HHHHHHHHCCCCCCCCEEEECCCCHHHCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCH
AKLRHLITFQQGLGLPVEWLNGAEVRRREPHLATKLAGAVWCPEDHQVDNRKVSAALRVA
HHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHCEEECCCCCCCCCHHHHHHHHHH
ALKAGATLHEDMPVERVEVVDGRARAVIAKGERFGADIVVLAAGAWSRGVAITPATPLPV
HHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCEEECCCCCCCC
RPVKGQMLALAMDPAAPILSHVVWAPGTYLVPRKDGRLIVGATVEERGFNADLTAGGQLA
CCCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCEE
LLTHAWRALPTIEELPILEQWVGFRPGSRDDAPILGRSAEVEGLVFATGHHRNGILLLPV
HHHHHHHHCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
TAEAISRLILEGETDPVIAPFVAERFLPRAAAE
CHHHHHHHHHCCCCCCEEHHHHHHHHCHHHCCC
>Mature Secondary Structure 
ALPPPRRDGAMVFLAAEASCGDNDLTAWGAARHARLRSHPSNLSGSCQRREEPMSESLS
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHH
HHDSASSSAHSARASGARPRVAIVGGGVMGLSIAWRLAEAGCTVEIFEQGRTGLGASHAA
HCCCCCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
AGMLAACAEAEPGEEGLLALNRESQRLWPSFAADLEAASGMAVDLRTDGTIVLALNADDA
HHHHHHHHCCCCCCCCEEEECCCCHHHCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCH
AKLRHLITFQQGLGLPVEWLNGAEVRRREPHLATKLAGAVWCPEDHQVDNRKVSAALRVA
HHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHCEEECCCCCCCCCHHHHHHHHHH
ALKAGATLHEDMPVERVEVVDGRARAVIAKGERFGADIVVLAAGAWSRGVAITPATPLPV
HHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCEEECCCCCCCC
RPVKGQMLALAMDPAAPILSHVVWAPGTYLVPRKDGRLIVGATVEERGFNADLTAGGQLA
CCCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCEE
LLTHAWRALPTIEELPILEQWVGFRPGSRDDAPILGRSAEVEGLVFATGHHRNGILLLPV
HHHHHHHHCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
TAEAISRLILEGETDPVIAPFVAERFLPRAAAE
CHHHHHHHHHCCCCCCEEHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]