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The map label for this gene is leuA [H]

Identifier: 158425169

GI number: 158425169

Start: 4070875

End: 4072455

Strand: Direct

Name: leuA [H]

Synonym: AZC_3545

Alternate gene names: 158425169

Gene position: 4070875-4072455 (Clockwise)

Preceding gene: 158425166

Following gene: 158425170

Centisome position: 75.81

GC content: 66.41

Gene sequence:

>1581_bases
ATGACCGAGACCACCGCCGCTACGCCCGCCGTCTCCACCGAGGCGACCGACCGCGTCATCATCTTCGACACCACCCTGCG
CGACGGCGAACAGTGCCCCGGCGCCTCCATGACCTTCGAGGAGAAGCTCGAGGTCGCGGAATTGCTGGAAACCATGGGCG
TCGATGTCATCGAGGCCGGCTTCCCCATCGCCTCCATCGGCGATTTCGAGTCGGTGGCCGAGATTGCCCGCCGCTCCAAG
ACGGCGATCATCGCCGGCCTCTCCCGCGCCGCCCTCAACGACATCGACCGCTGCGCGGAAGCGGTGAAGCAGGCCAAGCG
CGGCCGCATCCACACCTTCCTGTCCACCTCGCCGGTGCACATGAAGTACAAGCTCCAGAAGGAGCCCCACCAGGTGCTGG
AGATGATCGTCGCCTCGGTGACGCGCGCCCGGAACCATGTGGAGGATGTGGAGTGGTCGGCGGAGGACGGTACGCGCACC
GAGATGGACTTCCTCTGCCGCTGCGTGGAGGCCGCCATCAAGGCCGGCGCAACCACAATCAACATCCCCGACACCGTGGG
CTACACCACGCCGCAGGAATACGAGGCGCTCTTCCGGACGGTCCGCGAGCGCGTACCAAATTCCGACAAGGCCATCTTCT
CGGTCCATTGCCATAATGACCTCGGCATGGCGGTCGCCAACTCGCTGGCCGGCCTCGCCGGCGGCGCGCGGCAGATCGAA
TGCACCATCAACGGCATCGGCGAGCGGGCCGGCAATGCGGCGCTGGAAGAGGTGGTGATGGCCATCAACACCCGCCGCGA
CGTGCTGCCCTATCGCACCGGCATCGATGCCACCCTGCTCACCCGCGCCTCGAAGATGGTCTCGGGCGTGACGTCCTTCC
CGGTGCAGTACAACAAGGCCATCGTCGGCCGGAATGCCTTCGCCCATGAGAGCGGCATCCATCAGGACGGCATGCTCAAG
CACACCCAGACCTACGAGATCATGACCCCCGAGAGCGTGGGCGTGACCAAGACCTCGCTGGTGATGGGCAAGCACTCCGG
CCGCGCTGCCTTCCGCGACAAGCTGAAGGCGTTGGGCTACGAGCTGGGTGAGAATGCGCTGAACGACGCCTTCACCCGCT
TCAAGGACCTCGCCGATCGCAAGAAGGTCATTTACGACGAGGACATTGAGGCGCTGGTGGATCAGGGCATCGCCGCTGCC
TACGATCGCGTGAAGCTGCTGTCGCTCTCGGTGATCGCCGGCACCCGCGGCCCGCAGCGCGCCACGATGCGCATCGAAGT
GGATGGCCAGCCCCGCATTGAGGAAGCCGAGGGCAACGGCCCGGTGGATGCCACCTTCAACGCCATCAAGGCGCTGATCC
CGCACACCGCCAAGCTCGAGCTCTATCAGGTGCATGCCGTGACCGAGGGCACGGACGCGCAGGCCGAGGTCTCGGTGCGT
CTCGCAGAGGACGGCAAGGTGGTGACCGCCCGCGCGGCCGACCCCGACACGCTGGTCGCGTCCGCGCAGGCCTACATCAC
GGCGCTCAACAAGCTCTCCGTGAAGCGCCAGAGCGTGAACGCGCAGGCCGCCGCCAGCTGA

Upstream 100 bases:

>100_bases
GCCTGGGCACTCAGGGGATGCGGAAAAGCCAGACCGACGCACCCGAGGCCCTCTCTCCTGACAATCGGATCGAGACGGCC
CGTCCCGAAGGACCCGTGAC

Downstream 100 bases:

>100_bases
GCGGTGCCCGACACGACACATCCGAAGCCCTCGCCCTCCGGCGGGGGCTTTTTTGTCTGAGGACGGTCCATGCACCGCAC
CGGTGTTCTGCTCGCGCTCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTETTAATPAVSTEATDRVIIFDTTLRDGEQCPGASMTFEEKLEVAELLETMGVDVIEAGFPIASIGDFESVAEIARRSK
TAIIAGLSRAALNDIDRCAEAVKQAKRGRIHTFLSTSPVHMKYKLQKEPHQVLEMIVASVTRARNHVEDVEWSAEDGTRT
EMDFLCRCVEAAIKAGATTINIPDTVGYTTPQEYEALFRTVRERVPNSDKAIFSVHCHNDLGMAVANSLAGLAGGARQIE
CTINGIGERAGNAALEEVVMAINTRRDVLPYRTGIDATLLTRASKMVSGVTSFPVQYNKAIVGRNAFAHESGIHQDGMLK
HTQTYEIMTPESVGVTKTSLVMGKHSGRAAFRDKLKALGYELGENALNDAFTRFKDLADRKKVIYDEDIEALVDQGIAAA
YDRVKLLSLSVIAGTRGPQRATMRIEVDGQPRIEEAEGNGPVDATFNAIKALIPHTAKLELYQVHAVTEGTDAQAEVSVR
LAEDGKVVTARAADPDTLVASAQAYITALNKLSVKRQSVNAQAAAS

Sequences:

>Translated_526_residues
MTETTAATPAVSTEATDRVIIFDTTLRDGEQCPGASMTFEEKLEVAELLETMGVDVIEAGFPIASIGDFESVAEIARRSK
TAIIAGLSRAALNDIDRCAEAVKQAKRGRIHTFLSTSPVHMKYKLQKEPHQVLEMIVASVTRARNHVEDVEWSAEDGTRT
EMDFLCRCVEAAIKAGATTINIPDTVGYTTPQEYEALFRTVRERVPNSDKAIFSVHCHNDLGMAVANSLAGLAGGARQIE
CTINGIGERAGNAALEEVVMAINTRRDVLPYRTGIDATLLTRASKMVSGVTSFPVQYNKAIVGRNAFAHESGIHQDGMLK
HTQTYEIMTPESVGVTKTSLVMGKHSGRAAFRDKLKALGYELGENALNDAFTRFKDLADRKKVIYDEDIEALVDQGIAAA
YDRVKLLSLSVIAGTRGPQRATMRIEVDGQPRIEEAEGNGPVDATFNAIKALIPHTAKLELYQVHAVTEGTDAQAEVSVR
LAEDGKVVTARAADPDTLVASAQAYITALNKLSVKRQSVNAQAAAS
>Mature_525_residues
TETTAATPAVSTEATDRVIIFDTTLRDGEQCPGASMTFEEKLEVAELLETMGVDVIEAGFPIASIGDFESVAEIARRSKT
AIIAGLSRAALNDIDRCAEAVKQAKRGRIHTFLSTSPVHMKYKLQKEPHQVLEMIVASVTRARNHVEDVEWSAEDGTRTE
MDFLCRCVEAAIKAGATTINIPDTVGYTTPQEYEALFRTVRERVPNSDKAIFSVHCHNDLGMAVANSLAGLAGGARQIEC
TINGIGERAGNAALEEVVMAINTRRDVLPYRTGIDATLLTRASKMVSGVTSFPVQYNKAIVGRNAFAHESGIHQDGMLKH
TQTYEIMTPESVGVTKTSLVMGKHSGRAAFRDKLKALGYELGENALNDAFTRFKDLADRKKVIYDEDIEALVDQGIAAAY
DRVKLLSLSVIAGTRGPQRATMRIEVDGQPRIEEAEGNGPVDATFNAIKALIPHTAKLELYQVHAVTEGTDAQAEVSVRL
AEDGKVVTARAADPDTLVASAQAYITALNKLSVKRQSVNAQAAAS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=498, Percent_Identity=46.5863453815261, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320019, Length=394, Percent_Identity=26.9035532994924, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=27.8947368421053, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=497, Percent_Identity=25.9557344064386, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=501, Percent_Identity=25.1497005988024, Blast_Score=113, Evalue=9e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56833; Mature: 56702

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETTAATPAVSTEATDRVIIFDTTLRDGEQCPGASMTFEEKLEVAELLETMGVDVIEAG
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECC
FPIASIGDFESVAEIARRSKTAIIAGLSRAALNDIDRCAEAVKQAKRGRIHTFLSTSPVH
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
MKYKLQKEPHQVLEMIVASVTRARNHVEDVEWSAEDGTRTEMDFLCRCVEAAIKAGATTI
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NIPDTVGYTTPQEYEALFRTVRERVPNSDKAIFSVHCHNDLGMAVANSLAGLAGGARQIE
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEE
CTINGIGERAGNAALEEVVMAINTRRDVLPYRTGIDATLLTRASKMVSGVTSFPVQYNKA
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
IVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVTKTSLVMGKHSGRAAFRDKLKALGY
EECCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEEECCCCHHHHHHHHHHHHH
ELGENALNDAFTRFKDLADRKKVIYDEDIEALVDQGIAAAYDRVKLLSLSVIAGTRGPQR
HHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHEEEEEEECCCCCCC
ATMRIEVDGQPRIEEAEGNGPVDATFNAIKALIPHTAKLELYQVHAVTEGTDAQAEVSVR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEE
LAEDGKVVTARAADPDTLVASAQAYITALNKLSVKRQSVNAQAAAS
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TETTAATPAVSTEATDRVIIFDTTLRDGEQCPGASMTFEEKLEVAELLETMGVDVIEAG
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECC
FPIASIGDFESVAEIARRSKTAIIAGLSRAALNDIDRCAEAVKQAKRGRIHTFLSTSPVH
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
MKYKLQKEPHQVLEMIVASVTRARNHVEDVEWSAEDGTRTEMDFLCRCVEAAIKAGATTI
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NIPDTVGYTTPQEYEALFRTVRERVPNSDKAIFSVHCHNDLGMAVANSLAGLAGGARQIE
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEE
CTINGIGERAGNAALEEVVMAINTRRDVLPYRTGIDATLLTRASKMVSGVTSFPVQYNKA
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCE
IVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVTKTSLVMGKHSGRAAFRDKLKALGY
EECCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEEECCCCHHHHHHHHHHHHH
ELGENALNDAFTRFKDLADRKKVIYDEDIEALVDQGIAAAYDRVKLLSLSVIAGTRGPQR
HHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHEEEEEEECCCCCCC
ATMRIEVDGQPRIEEAEGNGPVDATFNAIKALIPHTAKLELYQVHAVTEGTDAQAEVSVR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEE
LAEDGKVVTARAADPDTLVASAQAYITALNKLSVKRQSVNAQAAAS
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA