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The map label for this gene is cca [H]

Identifier: 158425129

GI number: 158425129

Start: 4020752

End: 4021993

Strand: Reverse

Name: cca [H]

Synonym: AZC_3505

Alternate gene names: 158425129

Gene position: 4021993-4020752 (Counterclockwise)

Preceding gene: 158425130

Following gene: 158425125

Centisome position: 74.9

GC content: 73.67

Gene sequence:

>1242_bases
ATGAGCGGCCCGTCGCTGGCGGGGGCCGCCTTCCTTGCCGCACCCCCGTTGCGGGCGGTGCTGGCCGCGCTCAATGGCGA
CGGCGAGGAAACGCGCATCGTCGGCGGCGCGGTGCGCAACGGCCTGCTGGGCGAGGCCGTGGGCGAGGTGGATCTCGCCT
CCACCTGCCTGCCGGAGGAGGTGGTGCGTCGCGCCGAGGGCGCGGGCTTGAAGAGCGTGCCCACCGGCATTGCCCACGGT
ACCGTGACCGTGGTGAGCGCGGGCGTGCCCTTTGAGGTGACGACGCTCCGCGAGGATGTGGAGACGGACGGGCGCCATGC
GGTGGTGCGCTTCGGCCGCGACTGGGCGCGCGACGCCGCGCGCCGCGACTTCACCCTCAACGCCCTCTATGCGGATGCAG
ACGGCTTGGTCTTCGACCCTGTGGGTGGTCTGCCGGACCTCATGGCGCGCCGCGTGCGCTTCATCGGTGATGCCGAGACC
CGCATCCGCGAGGACCATCTGCGCATCCTCCGCCTGTTCCGGTTCCACGCCACCTATGCCAAGGGTCCCGTGGATGCCGA
GGCGCTCAGGGCCGCCGAGCGGCTGCGGGCCGGCCTCGACCAGCTCTCGCGGGAGCGGGTGCGGGCGGAGCTGGTGAAGC
TGCTCGTGGCCCGGCGGGCGGCGGACACGCTGGTGGAAATGACCGATGCCGGGCTGCTGACCCGCATCCTCGCGGGGGTG
CCGGACCTCTCCGGCTTTGCGCACCTCGCACGGCTTGAGCCTGAGCTTGGCATCCAGCCGGCACCCATGCGCCGGCTCGC
GGCGCTGGCTGTCCGGATTCCCGAGGACGCCGAGCGTCTGCGGGTCCGCCTGCGCCTTGCCAATGCAGAGCACGCCCGCC
TGCTGGCGCTCGCCGGCGCGCGGGCGGTGGGGCCGGGCCTCTCCGCTCCGGAGGCTCGGGCGTTGATCTTCGCGCTGGGA
GCGGACGTGTTCCGGGATGCCCTGCTGATCGCGGCGGCACGGCGGGGCGGCGATCCATCGGCGCTGCTGGCGCTCGCCGC
CGCATGGCCGGTTCCGGAATTCCCGCTGACCGCTCGGGATCTCATGGCCCTCGGGCTCAAGCCGGGACCGAAGCTCGGCG
CCGCGCTGGCGTCGGCGCGTGAGGCATGGGTGGCGGCGGATTTTCCGCATGACGCCGAAACCCTTGCGGGCATGGCGCGC
GCGGCGGCCGAAGCGGGAGGCGGCGGGCCGTCCTCCCGCTGA

Upstream 100 bases:

>100_bases
CGGGTCTGTGGCTGTTCGAGTGGGATCAGGGCGCGCGGCCGGGCAGCCGCTATGTGCCGGCCCATATGGAGAATGGCGTC
TTCGTGCCGGGCCGCTACGA

Downstream 100 bases:

>100_bases
ACGCAGGGCCGATGCGGCGCGACCGCAGGCTCAGTCCTCGTCTTCCTCGTCCCCGTCTTCTTCCACCTCGAACTCGGTGA
TGTCGAGGACTTCGAGGCCG

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSGPSLAGAAFLAAPPLRAVLAALNGDGEETRIVGGAVRNGLLGEAVGEVDLASTCLPEEVVRRAEGAGLKSVPTGIAHG
TVTVVSAGVPFEVTTLREDVETDGRHAVVRFGRDWARDAARRDFTLNALYADADGLVFDPVGGLPDLMARRVRFIGDAET
RIREDHLRILRLFRFHATYAKGPVDAEALRAAERLRAGLDQLSRERVRAELVKLLVARRAADTLVEMTDAGLLTRILAGV
PDLSGFAHLARLEPELGIQPAPMRRLAALAVRIPEDAERLRVRLRLANAEHARLLALAGARAVGPGLSAPEARALIFALG
ADVFRDALLIAAARRGGDPSALLALAAAWPVPEFPLTARDLMALGLKPGPKLGAALASAREAWVAADFPHDAETLAGMAR
AAAEAGGGGPSSR

Sequences:

>Translated_413_residues
MSGPSLAGAAFLAAPPLRAVLAALNGDGEETRIVGGAVRNGLLGEAVGEVDLASTCLPEEVVRRAEGAGLKSVPTGIAHG
TVTVVSAGVPFEVTTLREDVETDGRHAVVRFGRDWARDAARRDFTLNALYADADGLVFDPVGGLPDLMARRVRFIGDAET
RIREDHLRILRLFRFHATYAKGPVDAEALRAAERLRAGLDQLSRERVRAELVKLLVARRAADTLVEMTDAGLLTRILAGV
PDLSGFAHLARLEPELGIQPAPMRRLAALAVRIPEDAERLRVRLRLANAEHARLLALAGARAVGPGLSAPEARALIFALG
ADVFRDALLIAAARRGGDPSALLALAAAWPVPEFPLTARDLMALGLKPGPKLGAALASAREAWVAADFPHDAETLAGMAR
AAAEAGGGGPSSR
>Mature_412_residues
SGPSLAGAAFLAAPPLRAVLAALNGDGEETRIVGGAVRNGLLGEAVGEVDLASTCLPEEVVRRAEGAGLKSVPTGIAHGT
VTVVSAGVPFEVTTLREDVETDGRHAVVRFGRDWARDAARRDFTLNALYADADGLVFDPVGGLPDLMARRVRFIGDAETR
IREDHLRILRLFRFHATYAKGPVDAEALRAAERLRAGLDQLSRERVRAELVKLLVARRAADTLVEMTDAGLLTRILAGVP
DLSGFAHLARLEPELGIQPAPMRRLAALAVRIPEDAERLRVRLRLANAEHARLLALAGARAVGPGLSAPEARALIFALGA
DVFRDALLIAAARRGGDPSALLALAAAWPVPEFPLTARDLMALGLKPGPKLGAALASAREAWVAADFPHDAETLAGMARA
AAEAGGGGPSSR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=393, Percent_Identity=31.2977099236641, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI87081691, Length=227, Percent_Identity=33.4801762114537, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789436, Length=198, Percent_Identity=31.3131313131313, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71995920, Length=410, Percent_Identity=30.4878048780488, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI281360112, Length=389, Percent_Identity=32.6478149100257, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI21357337, Length=389, Percent_Identity=32.6478149100257, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 43412; Mature: 43281

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPSLAGAAFLAAPPLRAVLAALNGDGEETRIVGGAVRNGLLGEAVGEVDLASTCLPEE
CCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHEEECHHHHCCHHHHHHCCHHHHHHCCHHH
VVRRAEGAGLKSVPTGIAHGTVTVVSAGVPFEVTTLREDVETDGRHAVVRFGRDWARDAA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHCCCCCEEHHHHCHHHHHHHH
RRDFTLNALYADADGLVFDPVGGLPDLMARRVRFIGDAETRIREDHLRILRLFRFHATYA
HCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
KGPVDAEALRAAERLRAGLDQLSRERVRAELVKLLVARRAADTLVEMTDAGLLTRILAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDLSGFAHLARLEPELGIQPAPMRRLAALAVRIPEDAERLRVRLRLANAEHARLLALAGA
CCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCCHHHHHEEHHHCCCCCHHHHHHHHCC
RAVGPGLSAPEARALIFALGADVFRDALLIAAARRGGDPSALLALAAAWPVPEFPLTARD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LMALGLKPGPKLGAALASAREAWVAADFPHDAETLAGMARAAAEAGGGGPSSR
HHHHCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SGPSLAGAAFLAAPPLRAVLAALNGDGEETRIVGGAVRNGLLGEAVGEVDLASTCLPEE
CCCCCCCHHHHHCCHHHHHHHHHCCCCCHHEEECHHHHCCHHHHHHCCHHHHHHCCHHH
VVRRAEGAGLKSVPTGIAHGTVTVVSAGVPFEVTTLREDVETDGRHAVVRFGRDWARDAA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHCCCCCEEHHHHCHHHHHHHH
RRDFTLNALYADADGLVFDPVGGLPDLMARRVRFIGDAETRIREDHLRILRLFRFHATYA
HCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
KGPVDAEALRAAERLRAGLDQLSRERVRAELVKLLVARRAADTLVEMTDAGLLTRILAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDLSGFAHLARLEPELGIQPAPMRRLAALAVRIPEDAERLRVRLRLANAEHARLLALAGA
CCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCCHHHHHEEHHHCCCCCHHHHHHHHCC
RAVGPGLSAPEARALIFALGADVFRDALLIAAARRGGDPSALLALAAAWPVPEFPLTARD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LMALGLKPGPKLGAALASAREAWVAADFPHDAETLAGMARAAAEAGGGGPSSR
HHHHCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA