Click here to switch to the map view.

The map label for this gene is fixC

Identifier: 158425073

GI number: 158425073

Start: 3964583

End: 3965890

Strand: Direct

Name: fixC

Synonym: AZC_3449

Alternate gene names: 158425073

Gene position: 3964583-3965890 (Clockwise)

Preceding gene: 158425072

Following gene: 158425074

Centisome position: 73.83

GC content: 63.84

Gene sequence:

>1308_bases
ATGATTGATGAGAAGTTCGACGCCATCGTCGTCGGCGCGGGCATGGCGGGCAACGCCGCCACGCTCACCCTCGCCCGGCG
CGGCCTGAAGGTGCTGCAACTGGAGCGGGGCGAATATTCCGGCTCCAAGAATGTGCAGGGCGCCATTCTCTATGCGGACA
TGCTGGAGAAGCTGGTCCCCGATTTCCGCGAGGACGCGCCGCTGGAGCGCCATCTCGTGGAACAGCGCTTCTGGATGCTG
GACGACCGCTCCCATGTGGGCCTCCACTATCGCTCGGAGGACTTCAACGAGGAGAAGCCGAACCGCTACACCATCATCCG
CGCCCAGTTCGACAAGTGGTTCTCCTCCAAGGTGAAGGAGGCCGGCGCCACGGTGCTGTGCGAGACCACGGTGACGGAAC
TGGTGCAGGATGCCTATGGCAAGGTGATCGGGGTCAAGACCGATCGCTCCGGTGGCCAGATCCATGCGGATGTGGTGGTG
CTGGCGGAAGGCGTGAACGGCCTGCTCGGCACCAAGGCGCACCTGCGCGAACGGCCGAAGCCCGAGCATGTGGCGCTGGC
GGTGAAGGAGATGCATTTCCTGCCCCGCGAGACCATCGAAGCCCGCTTCAACCTCCAGGGCGACGAGGGGGTGGTCATCG
AGGCCGCCGGCACCATCTCCCGCGGCATGACGGGCATGGGCTTCATCTATGCCAACAAGGAATGCATCTCGCTGGGCATC
GGCTGCCTCGTCTCCGATTTCCAGAAGACCGGCGAGACGCCCTATGGCCTGCTGGAGCGCTTCAAGAGCCACCCCTCCGT
CGCGCCGCTCATCGAGGGGTCGGAAGTGAAGGAATATGCCGCGCACCTCATTCCCGAGGGTGGATTCAAGGCCATTCCCC
AGCTCTTCGGCGACGGCTGGGTGGTGGTGGGCGATGCCGCCCAGCTCAACAATGCCGTCCATCGCGAGGGGTCGAACCTC
GCCATGACCTCGGGCCGGATCGCGGCGGAAGCCATCTTCCAGGTGAAATCCCGCCGCGAGCCCATGAGCGCGAAGAACCT
CTCGCTCTACAAGACCATGCTGGAAGAGAGCTTCGTCCTGAAGGACATGAAGAAGTACAAGGACCTTCCGGCCCTGCTTC
ACATCAATTCGCAGAACTTCTTCCTCACCTATCCGCAGCTGGTCTCCAAGGCCATGCAGAACTTCGTGCGGGTGGATGGC
ACGCCCAAGGTCGAGAAGGAGAAGCGGACGCTGCGTGCCTTCGTGGAGGCCCGCTCCTGGTTCGGCCTGTTCGGCGATGC
CTTCCGCTTCGCCCGCGCGTGGCGGTGA

Upstream 100 bases:

>100_bases
TCACCGATGCGATCCGGCTGCTGCCGGCGCTGACCGAAGCCTTCCGCGCCCGCCTCTCCCCCCATTCGCGCGACCGGATC
GCCAGCTGAAGGAGCGTGCC

Downstream 100 bases:

>100_bases
GCCCGCCCGGTCGAAAGGTTTCGAGTGACATGCGTTCCCGGTCCGCTGCCCCCGCAGCGGGCGTTCCGCCACCGGCCTGA
GGCCGGGCGAGACAAGGAGA

Product: protein fixC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIDEKFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEYSGSKNVQGAILYADMLEKLVPDFREDAPLERHLVEQRFWML
DDRSHVGLHYRSEDFNEEKPNRYTIIRAQFDKWFSSKVKEAGATVLCETTVTELVQDAYGKVIGVKTDRSGGQIHADVVV
LAEGVNGLLGTKAHLRERPKPEHVALAVKEMHFLPRETIEARFNLQGDEGVVIEAAGTISRGMTGMGFIYANKECISLGI
GCLVSDFQKTGETPYGLLERFKSHPSVAPLIEGSEVKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNL
AMTSGRIAAEAIFQVKSRREPMSAKNLSLYKTMLEESFVLKDMKKYKDLPALLHINSQNFFLTYPQLVSKAMQNFVRVDG
TPKVEKEKRTLRAFVEARSWFGLFGDAFRFARAWR

Sequences:

>Translated_435_residues
MIDEKFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEYSGSKNVQGAILYADMLEKLVPDFREDAPLERHLVEQRFWML
DDRSHVGLHYRSEDFNEEKPNRYTIIRAQFDKWFSSKVKEAGATVLCETTVTELVQDAYGKVIGVKTDRSGGQIHADVVV
LAEGVNGLLGTKAHLRERPKPEHVALAVKEMHFLPRETIEARFNLQGDEGVVIEAAGTISRGMTGMGFIYANKECISLGI
GCLVSDFQKTGETPYGLLERFKSHPSVAPLIEGSEVKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNL
AMTSGRIAAEAIFQVKSRREPMSAKNLSLYKTMLEESFVLKDMKKYKDLPALLHINSQNFFLTYPQLVSKAMQNFVRVDG
TPKVEKEKRTLRAFVEARSWFGLFGDAFRFARAWR
>Mature_435_residues
MIDEKFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEYSGSKNVQGAILYADMLEKLVPDFREDAPLERHLVEQRFWML
DDRSHVGLHYRSEDFNEEKPNRYTIIRAQFDKWFSSKVKEAGATVLCETTVTELVQDAYGKVIGVKTDRSGGQIHADVVV
LAEGVNGLLGTKAHLRERPKPEHVALAVKEMHFLPRETIEARFNLQGDEGVVIEAAGTISRGMTGMGFIYANKECISLGI
GCLVSDFQKTGETPYGLLERFKSHPSVAPLIEGSEVKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNL
AMTSGRIAAEAIFQVKSRREPMSAKNLSLYKTMLEESFVLKDMKKYKDLPALLHINSQNFFLTYPQLVSKAMQNFVRVDG
TPKVEKEKRTLRAFVEARSWFGLFGDAFRFARAWR

Specific function: Could be required for the formation of a functional nitrogenase Fe protein. Probably accepts electrons from fixA/fixB and reduces a quinone

COG id: COG0644

COG function: function code C; Dehydrogenases (flavoproteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF-QO/fixC family

Homologues:

Organism=Homo sapiens, GI119703746, Length=403, Percent_Identity=25.8064516129032, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1787991, Length=436, Percent_Identity=38.0733944954128, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1786227, Length=414, Percent_Identity=38.4057971014493, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082155, Length=423, Percent_Identity=33.806146572104, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25144720, Length=393, Percent_Identity=23.4096692111959, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324932, Length=415, Percent_Identity=26.7469879518072, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652256, Length=401, Percent_Identity=24.6882793017456, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXC_AZOC5 (P26484)

Other databases:

- EMBL:   X55450
- EMBL:   AP009384
- PIR:   S14072
- RefSeq:   YP_001526365.1
- ProteinModelPortal:   P26484
- SMR:   P26484
- GeneID:   5690798
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3449
- HOGENOM:   HBG295204
- OMA:   ILYADML
- ProtClustDB:   CLSK864405
- BioCyc:   ACAU438753:AZC_3449-MONOMER
- InterPro:   IPR002938

Pfam domain/function: PF01494 FAD_binding_3

EC number: 1.5.5.-

Molecular weight: Translated: 48531; Mature: 48531

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDEKFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEYSGSKNVQGAILYADMLEKLVP
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHCC
DFREDAPLERHLVEQRFWMLDDRSHVGLHYRSEDFNEEKPNRYTIIRAQFDKWFSSKVKE
CHHCCCCHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHH
AGATVLCETTVTELVQDAYGKVIGVKTDRSGGQIHADVVVLAEGVNGLLGTKAHLRERPK
CCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEEECCCCCHHCCHHHHHCCCC
PEHVALAVKEMHFLPRETIEARFNLQGDEGVVIEAAGTISRGMTGMGFIYANKECISLGI
CHHHHHHHHHHHHCCHHHHHHEEECCCCCCEEEEECCCHHCCCCCCEEEEECHHHHHHHH
GCLVSDFQKTGETPYGLLERFKSHPSVAPLIEGSEVKEYAAHLIPEGGFKAIPQLFGDGW
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCE
VVVGDAAQLNNAVHREGSNLAMTSGRIAAEAIFQVKSRREPMSAKNLSLYKTMLEESFVL
EEECCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KDMKKYKDLPALLHINSQNFFLTYPQLVSKAMQNFVRVDGTPKVEKEKRTLRAFVEARSW
HHHHHHCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FGLFGDAFRFARAWR
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDEKFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEYSGSKNVQGAILYADMLEKLVP
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHCC
DFREDAPLERHLVEQRFWMLDDRSHVGLHYRSEDFNEEKPNRYTIIRAQFDKWFSSKVKE
CHHCCCCHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHH
AGATVLCETTVTELVQDAYGKVIGVKTDRSGGQIHADVVVLAEGVNGLLGTKAHLRERPK
CCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEEECCCCCHHCCHHHHHCCCC
PEHVALAVKEMHFLPRETIEARFNLQGDEGVVIEAAGTISRGMTGMGFIYANKECISLGI
CHHHHHHHHHHHHCCHHHHHHEEECCCCCCEEEEECCCHHCCCCCCEEEEECHHHHHHHH
GCLVSDFQKTGETPYGLLERFKSHPSVAPLIEGSEVKEYAAHLIPEGGFKAIPQLFGDGW
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCE
VVVGDAAQLNNAVHREGSNLAMTSGRIAAEAIFQVKSRREPMSAKNLSLYKTMLEESFVL
EEECCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KDMKKYKDLPALLHINSQNFFLTYPQLVSKAMQNFVRVDGTPKVEKEKRTLRAFVEARSW
HHHHHHCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FGLFGDAFRFARAWR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1850088