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The map label for this gene is nifH

Identifier: 158425067

GI number: 158425067

Start: 3959441

End: 3960331

Strand: Direct

Name: nifH

Synonym: AZC_3443

Alternate gene names: 158425067

Gene position: 3959441-3960331 (Clockwise)

Preceding gene: 158425062

Following gene: 158425068

Centisome position: 73.74

GC content: 64.2

Gene sequence:

>891_bases
ATGTCGTCACTGAGACAGATCGCATTTTACGGCAAGGGGGGCATCGGCAAGTCGACGACGTCCCAGAACACGCTGGCGGC
GCTGGCGGAGATGGGCCATCGCATCCTCATCGTCGGCTGCGACCCCAAGGCCGACTCAACCCGCCTCATCCTGCACGCCA
AGGCGCAGGACACCATCCTGTCGCTGGCCGCCGCCGCCGGCAGCGTCGAAGACCTCGAGCTCGAAGAGGTCATGAAGATC
GGCTACCGCGACATCCGCTGCGTGGAATCCGGCGGCCCGGAGCCGGGCGTCGGCTGCGCGGGGCGCGGCGTCATCACCTC
CATCAATTTCCTGGAGGAGAACGGCGCCTATGAGGACATCGACTACGTCTCCTACGACGTGCTCGGCGACGTGGTGTGCG
GCGGCTTCGCCATGCCCATCCGCGAGAACAAGGCGCAGGAAATCTACATCGTCATGTCCGGCGAGATGATGGCCATGTAT
GCGGCCAACAACATCTCCAAGGGCATTCTCAAGTACGCCAATTCCGGCGGCGTGCGCCTGGGCGGGCTCGTCTGCAACGA
GCGCCAGACGGACAAGGAGCTGGAACTGGCCGAGAACCTCGCCAAGAAGCTCGGCACCCAGCTCATCTATTTCGTGCCGC
GCGACAACATCGTGCAGCACGCCGAGCTGCGCCGCATGACGGTGATCGAGTATGCGCCCGACAGCGTTCAGGCGAACCAC
TATCGCAACCTCGCTGAGCGCGTCCACAACAATGGCGGCAAGGGCATCATCCCGACCCCCATCACCATGGACGAGCTCGA
GGACATGCTCATGGAGCACGGCATCATGAAGACGGTGGACGAGACCCAGGTCGGCAAGACCGCCGCCGAACTCGCCGCGC
TCTCCGCGTGA

Upstream 100 bases:

>100_bases
GCGATGTGCTCCTTCCCGTAACCGACGAGCCTGTTTGTGAGCGCGAGCGCCCCAGCCCCGGCGGGGTGGTCATTGGCAAC
GGTTCGAAGGAATTCAAGAC

Downstream 100 bases:

>100_bases
CGCCCCCGTCCCCGGCCGCCGGCCGGGGACATCCGTTCATGCCCGAGCCTTTCATCCCAAGACGTTTGATTGAGACCCTT
CATTCTGACCGACGATCCGC

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 1; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTILSLAAAAGSVEDLELEEVMKI
GYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
AANNISKGILKYANSGGVRLGGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDETQVGKTAAELAALSA

Sequences:

>Translated_296_residues
MSSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTILSLAAAAGSVEDLELEEVMKI
GYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
AANNISKGILKYANSGGVRLGGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDETQVGKTAAELAALSA
>Mature_295_residues
SSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTILSLAAAAGSVEDLELEEVMKIG
YRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYA
ANNISKGILKYANSGGVRLGGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANHY
RNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDETQVGKTAAELAALSA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH1_AZOC5 (P26251)

Other databases:

- EMBL:   M16709
- EMBL:   AP009384
- PIR:   B47622
- RefSeq:   YP_001526359.1
- ProteinModelPortal:   P26251
- SMR:   P26251
- GeneID:   5688598
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3443
- HOGENOM:   HBG488165
- OMA:   SDENIRQ
- ProtClustDB:   PRK13234
- BioCyc:   ACAU438753:AZC_3443-MONOMER
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 32002; Mature: 31871

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTIL
CCCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHH
SLAAAAGSVEDLELEEVMKIGYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDI
HHHHHCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEE
GGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
EEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHEEEEEECCCCCCHHH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDETQVGKTAAELAALSA
HHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTIL
CCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHH
SLAAAAGSVEDLELEEVMKIGYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDI
HHHHHCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEE
GGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
EEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHEEEEEECCCCCCHHH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDETQVGKTAAELAALSA
HHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA