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The map label for this gene is nifB [H]

Identifier: 158425038

GI number: 158425038

Start: 3941201

End: 3942760

Strand: Direct

Name: nifB [H]

Synonym: AZC_3414

Alternate gene names: 158425038

Gene position: 3941201-3942760 (Clockwise)

Preceding gene: 158425037

Following gene: 158425039

Centisome position: 73.4

GC content: 64.04

Gene sequence:

>1560_bases
ATGCACGGCTTGACACAGGACGTCAGCAGCGCCGGAAGCGCTCCCGACATGGGTGAGATCATGCAGGCCATTGCCGAACA
CAAAGGCTGTGGCACCTCGGGCGGCAGCGGCAAGGCCAGCTGCGGCTCCGGCGCGGGTCAGGGCGACCTGCCCACCGAAA
TCTGGGAGAAGGTGAAGAACCATCCCTGCTACAGCGAGGAAGCCCACCACCATTACGCCCGCATGCATGTGGCCGTCGCC
CCGGCGTGCAACATCCAGTGCAACTACTGCAACCGCAAGTATGATTGCGCGAACGAGAGCCGGCCCGGCGTGGTGAGCGA
GAAGCTCTCCCCCGAGCAGGCGGCCAAGAAGGTGCTGGCGGTGGCCTCCACCATCCCCCAGATGACGGTGCTCGGCATCG
CCGGCCCGGGCGACCCGCTCGCCAATCCGGAGAAGACCTTCAAGACCTTCGAACTGATTTCGAAGACCGCGCCGGACATC
AAGCTCTGCCTGTCCACTAACGGCCTCACCCTGCCGGACCACGTGGACACCATCGCCAGCTACAATGTCGATCACGTGAC
CATCACCATCAACATGGTGGATCCCGAGATCGGCGCGAAGATCTACCCCTGGGTCTTCTACAAGCACAAGCGCTACACCG
GCATCGACGCGGCCAAGATCCTCACCGATCGCCAGTTGCTCGGCCTCGAGATGCTCACCGCCCGCGGCATCCTCACCAAG
ATCAACTCGGTGATGATCCCCGGCATCAACGACCAGCATCTGGTCGAGGTGAACAAGGCGGTGAAGTCGCGCGGCGCCTT
CCTGCACAACATCATGCCCCTCATCTCCGCCCCCGAGCACGGCACCGTCTTCGGCCTCAACGGCCAGCGCGGCCCGACCG
CCCAGGAGCTGAAGGCCCTGCAGGACAGCTGCGAGGGCGAGATGAACATGATGCGTCACTGCCGCCAGTGCCGCGCCGAT
GCGGTGGGCCTGCTCGGCGAAGACCGCAGCGCAGAATTCACCACCGACAAGATCATGGCGATGGAGGTGAATTACGACAT
GGAAGGCCGCAAGGCCTACCAGGCCGCGGTGGAAGAAGAGCGCGTCGCCAAGGTCGCGGCCAAGCAGGAAGAGCTGGCCG
GCCTCGCTGGCGAGATGAGCGACATCAAGCTTCTGGTGGCGGTAGCGACCAAGGGCTCGGGCCTCATCAACGAGCACTTC
GGCCATGCCAAGGAATTCCAGGTCTATGAGCTCTCCACGGCGGGCGCCAAGTTCGTCGGTCATCGCCGCGTCGATCTCTA
TTGCCAGGGCGGCTATGGCGAGGAGGACAGCCTGGAGACCATCATCCGCGCCATCAACGACTGCCATGCGGTGTTCGTGG
CGAAGATCGGCGGCTGCCCGAAGAGCGACCTGCTGAAGGCCGGCATCGAGCCGGTGGACCAGTTCGCCCACGAGTTCATC
GAGAAATCGGCCATCGGCTGGTTCAAGGATTATCTCGCGAAGGTCAATTCGGGCGAGATCGAGCACGTGGCGCGTGGCGA
TGCCGCCATTCGCCAGGGCGCGCTCATCTCGGCCGCCTGA

Upstream 100 bases:

>100_bases
TTTGATGTTCAACGCTTCCGGCTCCTCCCGCCGTGGTGGCACGGCTGTTGCTGGTATCTCCTCAGTAATATTGCGGATCG
GCCAGCAGCGAGGAACGGAC

Downstream 100 bases:

>100_bases
GCGAACGATCGGGAGGCAGCCATGGCACTCAAGATCGTCTCATCCCAGTGCACCAGCTGTTCGGCCTGCGAGCCGGAATG
CCCGAACGTGGCCATCAGCG

Product: nitrogenase cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MHGLTQDVSSAGSAPDMGEIMQAIAEHKGCGTSGGSGKASCGSGAGQGDLPTEIWEKVKNHPCYSEEAHHHYARMHVAVA
PACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELISKTAPDI
KLCLSTNGLTLPDHVDTIASYNVDHVTITINMVDPEIGAKIYPWVFYKHKRYTGIDAAKILTDRQLLGLEMLTARGILTK
INSVMIPGINDQHLVEVNKAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKALQDSCEGEMNMMRHCRQCRAD
AVGLLGEDRSAEFTTDKIMAMEVNYDMEGRKAYQAAVEEERVAKVAAKQEELAGLAGEMSDIKLLVAVATKGSGLINEHF
GHAKEFQVYELSTAGAKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKSDLLKAGIEPVDQFAHEFI
EKSAIGWFKDYLAKVNSGEIEHVARGDAAIRQGALISAA

Sequences:

>Translated_519_residues
MHGLTQDVSSAGSAPDMGEIMQAIAEHKGCGTSGGSGKASCGSGAGQGDLPTEIWEKVKNHPCYSEEAHHHYARMHVAVA
PACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELISKTAPDI
KLCLSTNGLTLPDHVDTIASYNVDHVTITINMVDPEIGAKIYPWVFYKHKRYTGIDAAKILTDRQLLGLEMLTARGILTK
INSVMIPGINDQHLVEVNKAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKALQDSCEGEMNMMRHCRQCRAD
AVGLLGEDRSAEFTTDKIMAMEVNYDMEGRKAYQAAVEEERVAKVAAKQEELAGLAGEMSDIKLLVAVATKGSGLINEHF
GHAKEFQVYELSTAGAKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKSDLLKAGIEPVDQFAHEFI
EKSAIGWFKDYLAKVNSGEIEHVARGDAAIRQGALISAA
>Mature_519_residues
MHGLTQDVSSAGSAPDMGEIMQAIAEHKGCGTSGGSGKASCGSGAGQGDLPTEIWEKVKNHPCYSEEAHHHYARMHVAVA
PACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELISKTAPDI
KLCLSTNGLTLPDHVDTIASYNVDHVTITINMVDPEIGAKIYPWVFYKHKRYTGIDAAKILTDRQLLGLEMLTARGILTK
INSVMIPGINDQHLVEVNKAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKALQDSCEGEMNMMRHCRQCRAD
AVGLLGEDRSAEFTTDKIMAMEVNYDMEGRKAYQAAVEEERVAKVAAKQEELAGLAGEMSDIKLLVAVATKGSGLINEHF
GHAKEFQVYELSTAGAKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKSDLLKAGIEPVDQFAHEFI
EKSAIGWFKDYLAKVNSGEIEHVARGDAAIRQGALISAA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56032; Mature: 56032

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGLTQDVSSAGSAPDMGEIMQAIAEHKGCGTSGGSGKASCGSGAGQGDLPTEIWEKVKN
CCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
HPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLA
CCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHH
VASTIPQMTVLGIAGPGDPLANPEKTFKTFELISKTAPDIKLCLSTNGLTLPDHVDTIAS
HHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
YNVDHVTITINMVDPEIGAKIYPWVFYKHKRYTGIDAAKILTDRQLLGLEMLTARGILTK
CCCCEEEEEEEEECCCCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
INSVMIPGINDQHLVEVNKAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKAL
HCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
QDSCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDMEGRKAYQAAVEEE
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
RVAKVAAKQEELAGLAGEMSDIKLLVAVATKGSGLINEHFGHAKEFQVYELSTAGAKFVG
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHCCCCCCEEEEEECCCCHHHHC
HRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKSDLLKAGIEPVDQFAHEFI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHH
EKSAIGWFKDYLAKVNSGEIEHVARGDAAIRQGALISAA
HHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHCCCEECCC
>Mature Secondary Structure
MHGLTQDVSSAGSAPDMGEIMQAIAEHKGCGTSGGSGKASCGSGAGQGDLPTEIWEKVKN
CCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
HPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLSPEQAAKKVLA
CCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHH
VASTIPQMTVLGIAGPGDPLANPEKTFKTFELISKTAPDIKLCLSTNGLTLPDHVDTIAS
HHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
YNVDHVTITINMVDPEIGAKIYPWVFYKHKRYTGIDAAKILTDRQLLGLEMLTARGILTK
CCCCEEEEEEEEECCCCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
INSVMIPGINDQHLVEVNKAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKAL
HCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
QDSCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDMEGRKAYQAAVEEE
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
RVAKVAAKQEELAGLAGEMSDIKLLVAVATKGSGLINEHFGHAKEFQVYELSTAGAKFVG
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHCCCCCCEEEEEECCCCHHHHC
HRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKSDLLKAGIEPVDQFAHEFI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHH
EKSAIGWFKDYLAKVNSGEIEHVARGDAAIRQGALISAA
HHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]