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The map label for this gene is nifS [H]

Identifier: 158425035

GI number: 158425035

Start: 3938554

End: 3939705

Strand: Direct

Name: nifS [H]

Synonym: AZC_3411

Alternate gene names: 158425035

Gene position: 3938554-3939705 (Clockwise)

Preceding gene: 158425034

Following gene: 158425036

Centisome position: 73.35

GC content: 69.27

Gene sequence:

>1152_bases
GTGCCGATCTATCTCGACAACAACGCGACGACGCGGGTCGATCCCGCCGTCGTCGCGGCCATGTTGCCCTTTTTCACCGA
GCAGTTCGGCAATGCCTCCTCCTCCCACGCGTTCGGCAGCAGCGTTGCCGAAAGCGTGCGCGCCGCCCGTCGCAGCCTTC
AGGCGCTGATTGGCGCGGCGTTTGATCATGAGATCGTCTTCACCTCCGGCGGGACGGAATCGGACAACACCGCCATCCTT
TCCGCCCTCGCCACGCAGGAGGGACGGGACGAGATCGTCACCAGCGCGGTCGAGCACCCGGCGGTGCTGAACCTGCTGGA
TCATCTGGAAGCCACGCGCGGCATCACCGTTCATCGCATCGGTGTCGATGGCGAGGGACGGCTTGATCTTGAGGCCTTCG
CCCGCGCGCTCTCTCCGCGCACGGCGCTCGCCACCATCATGTGGGCGAACAATGAGACGGGCACCCTGTTCCCCGTCGCC
CAGCTGGCGCAGATGGCCCGCGCCGTCGGCGCGCTGTTCCACACGGACGCGGTTCAGGCGGTCGGCAAGATACCGATCGA
TCTGGCGGCGACGCAGATCGACATGCTTTCGCTCTCCGGCCACAAGCTGCACGGGCCAAAAGGCATCGGCGCCCTCTATC
TGCGCAAAGGCACGCCTTTCGCCCCGCTGGTGCGTGGCGGCCCGCAGGAGCGGCGGCGGCGCGGCGGCACGGAGAATGTG
CCGGGCATCGTCGGCCTCGGGGCGGCGGCGGCGCTGGCGCTCGAAGAGCTGCCCGAGGAACAGGGCCGCGTGCGCGCGCT
GCGCGACCGGCTGGAACAGGGCGTGCTCCAGCAGGCCGGCCCCTGCCGGGCGCTGGGCGTGCCGGACGGACGCCTGCCCA
ACACCACCAACATCGCCTTCGAGCATCTGGAGGGCGAGGCGGTACTGCACAATCTCAACAAGGCGGGCATCGCCGCCTCG
CTCGGCTCCGCCTGCGCCTCCGGCTCCATGGAGCCGTCCCATGTGCTGAAAGCCATGCAGGTGCCGGCGAGCGCGCTGCG
CGGGGCCATCCGCTTCTCGCTCTCGCGCGACAATACGGACGACGACGTCGACCGGGTTCTGCAGGTGCTGCCGGAAATCA
CCGCCAGGCTGCGGTCCACCGTGCCGGCCTGA

Upstream 100 bases:

>100_bases
ACGCTGGAGGGCATCCAGTCCCGCCTCGTGGAGCGGCTTGGAGAGCTCGTGCGCGTGGTGCCGGTCGCCGGCGCTGCGAA
GCACTGAGCGGGACGGAGCC

Downstream 100 bases:

>100_bases
CCGGCGCGCCGCTTTCTCTGGCGAGCGCGCGATCAGTGATCGCACTCTAATTTATCGAATAGAAACGGATTCTGCCCGCA
CATTGAAGCATCAAAGCAAT

Product: nitrogenase cofactor synthesis protein

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPIYLDNNATTRVDPAVVAAMLPFFTEQFGNASSSHAFGSSVAESVRAARRSLQALIGAAFDHEIVFTSGGTESDNTAIL
SALATQEGRDEIVTSAVEHPAVLNLLDHLEATRGITVHRIGVDGEGRLDLEAFARALSPRTALATIMWANNETGTLFPVA
QLAQMARAVGALFHTDAVQAVGKIPIDLAATQIDMLSLSGHKLHGPKGIGALYLRKGTPFAPLVRGGPQERRRRGGTENV
PGIVGLGAAAALALEELPEEQGRVRALRDRLEQGVLQQAGPCRALGVPDGRLPNTTNIAFEHLEGEAVLHNLNKAGIAAS
LGSACASGSMEPSHVLKAMQVPASALRGAIRFSLSRDNTDDDVDRVLQVLPEITARLRSTVPA

Sequences:

>Translated_383_residues
MPIYLDNNATTRVDPAVVAAMLPFFTEQFGNASSSHAFGSSVAESVRAARRSLQALIGAAFDHEIVFTSGGTESDNTAIL
SALATQEGRDEIVTSAVEHPAVLNLLDHLEATRGITVHRIGVDGEGRLDLEAFARALSPRTALATIMWANNETGTLFPVA
QLAQMARAVGALFHTDAVQAVGKIPIDLAATQIDMLSLSGHKLHGPKGIGALYLRKGTPFAPLVRGGPQERRRRGGTENV
PGIVGLGAAAALALEELPEEQGRVRALRDRLEQGVLQQAGPCRALGVPDGRLPNTTNIAFEHLEGEAVLHNLNKAGIAAS
LGSACASGSMEPSHVLKAMQVPASALRGAIRFSLSRDNTDDDVDRVLQVLPEITARLRSTVPA
>Mature_382_residues
PIYLDNNATTRVDPAVVAAMLPFFTEQFGNASSSHAFGSSVAESVRAARRSLQALIGAAFDHEIVFTSGGTESDNTAILS
ALATQEGRDEIVTSAVEHPAVLNLLDHLEATRGITVHRIGVDGEGRLDLEAFARALSPRTALATIMWANNETGTLFPVAQ
LAQMARAVGALFHTDAVQAVGKIPIDLAATQIDMLSLSGHKLHGPKGIGALYLRKGTPFAPLVRGGPQERRRRGGTENVP
GIVGLGAAAALALEELPEEQGRVRALRDRLEQGVLQQAGPCRALGVPDGRLPNTTNIAFEHLEGEAVLHNLNKAGIAASL
GSACASGSMEPSHVLKAMQVPASALRGAIRFSLSRDNTDDDVDRVLQVLPEITARLRSTVPA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=38.8174807197943, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=36.4734299516908, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=41.9847328244275, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=29.8994974874372, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789175, Length=386, Percent_Identity=28.2383419689119, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=39.7435897435897, Blast_Score=264, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI17533177, Length=320, Percent_Identity=31.25, Blast_Score=151, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=38.974358974359, Blast_Score=255, Evalue=8e-69,
Organism=Drosophila melanogaster, GI20129463, Length=386, Percent_Identity=39.119170984456, Blast_Score=247, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40360; Mature: 40229

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIYLDNNATTRVDPAVVAAMLPFFTEQFGNASSSHAFGSSVAESVRAARRSLQALIGAA
CCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FDHEIVFTSGGTESDNTAILSALATQEGRDEIVTSAVEHPAVLNLLDHLEATRGITVHRI
CCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEE
GVDGEGRLDLEAFARALSPRTALATIMWANNETGTLFPVAQLAQMARAVGALFHTDAVQA
CCCCCCCCCHHHHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGKIPIDLAATQIDMLSLSGHKLHGPKGIGALYLRKGTPFAPLVRGGPQERRRRGGTENV
HCCCCHHHHHHHHHHEECCCCCCCCCCCCCEEEEECCCCCCHHHCCCHHHHHHCCCCCCC
PGIVGLGAAAALALEELPEEQGRVRALRDRLEQGVLQQAGPCRALGVPDGRLPNTTNIAF
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHH
EHLEGEAVLHNLNKAGIAASLGSACASGSMEPSHVLKAMQVPASALRGAIRFSLSRDNTD
HHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHEEEECCCCCH
DDVDRVLQVLPEITARLRSTVPA
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIYLDNNATTRVDPAVVAAMLPFFTEQFGNASSSHAFGSSVAESVRAARRSLQALIGAA
CEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FDHEIVFTSGGTESDNTAILSALATQEGRDEIVTSAVEHPAVLNLLDHLEATRGITVHRI
CCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEE
GVDGEGRLDLEAFARALSPRTALATIMWANNETGTLFPVAQLAQMARAVGALFHTDAVQA
CCCCCCCCCHHHHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGKIPIDLAATQIDMLSLSGHKLHGPKGIGALYLRKGTPFAPLVRGGPQERRRRGGTENV
HCCCCHHHHHHHHHHEECCCCCCCCCCCCCEEEEECCCCCCHHHCCCHHHHHHCCCCCCC
PGIVGLGAAAALALEELPEEQGRVRALRDRLEQGVLQQAGPCRALGVPDGRLPNTTNIAF
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHH
EHLEGEAVLHNLNKAGIAASLGSACASGSMEPSHVLKAMQVPASALRGAIRFSLSRDNTD
HHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHEEEECCCCCH
DDVDRVLQVLPEITARLRSTVPA
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]