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The map label for this gene is phnI [H]

Identifier: 158425018

GI number: 158425018

Start: 3924476

End: 3925591

Strand: Direct

Name: phnI [H]

Synonym: AZC_3394

Alternate gene names: 158425018

Gene position: 3924476-3925591 (Clockwise)

Preceding gene: 158425017

Following gene: 158425019

Centisome position: 73.08

GC content: 69.0

Gene sequence:

>1116_bases
ATGTATGTGGCCGTCAAGGGTGGCGAGACCGCCATCCGCAACGCCCATGCCCTGCTCGCCGAGACCCGGCGTGGGGATAC
GTCGGTGCCGGAGATCGGCATCGACCAGATCGCCGGCCAGCTCACGCTGGCGGTGGATCGCGTGATGAGCGAAGGCTCGC
TTTATGACCGCCCACTCGCCGCGCTTGCCATCAAGCAGGCGCGCGGCGATCTCATCGAGGCCATCTTCCTCGTGCGCGCC
TTCCGCACCACCTTGCCCCGCTTCGGCTATAGCGAGCCGGTGGACACGGGCCGGATGCGCGTGGAACGGCGCATCTCCGC
CACCTACAAGGACATTCCGGGCGGCCAGGTGCTCGGCCCCACCTTCGATTACACCCATCGCCTGCTGGACCCGACGCTCG
CGACGACCGGCGGCGCGGCCGGTGCGCCCAAGGGCCAGCCCGCACGCGCGGACGGCGCGGCGGAGCCCGGTCCCATGCCC
CGCGTCACCGATCTTCTGGGCGACGAGGGGCTGATCGAGCCGGCGCCCGCCACCGGCGAGGAGCCGCGCGATCTCACCCG
GGAACCCTTGAGCTTCCCCGCCGACCGTCCCCTCCGCCTCCAGAATCTCGCGCGGGGCGATGAGGGCTTCCTGCTCTCGC
TCGGCTATTCCACCCAGCGCGGCTATGCCCGCAGCCATCCCTTCGTGGGCGAGATCCGCTTCGGCACCGTCGAGGTGGAG
CTGTTCGTGGAGGAACTGGGCTTTGCGGTGCCGCTCGGCGACATCGCGCTCACCGAGTGCCAGATGGTGAACCAGTTCAA
GGGCTCCGCCAGCGAGCCGCCGAAGTTCACGCGCGGCTATGGCCTCGTGTTCGGCCAGAGCGAGCGCAAGGCCATGTCGG
TGGCGCTGGTGGATCGCGCGCTCCGCTTCAAGGAACTGGGCGAAGAACAGTCCGCCCCCGCGCAGGACGAGGAATTTGTC
CTCTCCCACTCCGACAATGTGCAGGCCACCGGCTTCGTGGAGCATCTGAAGCTGCCGCACTACGTGGATTTCCAGGCCGA
ACTCGACCTCGTGCGCCGGATGCGCGCCGAGCATGCGGACGGCACCGAGACCAGCCAGCAGGAGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
CCGGCTGCTGTTTCCGCTGGGTGTCGATCTCATCTTCACCACGTCAGGGCTGGTGTCGGCCCTGCCGCGCTCCACCCGCA
TCACCGCGGAGGCCTGATCC

Downstream 100 bases:

>100_bases
ACGCGCCCTTCGAAACCGCACAGGCGTCAACGCCCGCCGGCATGCCGGCCATCACCTACAATTTTGCCTATCTGGACGAG
CAGACGAAAAGGATGATCCG

Product: phosphonate metabolism protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MYVAVKGGETAIRNAHALLAETRRGDTSVPEIGIDQIAGQLTLAVDRVMSEGSLYDRPLAALAIKQARGDLIEAIFLVRA
FRTTLPRFGYSEPVDTGRMRVERRISATYKDIPGGQVLGPTFDYTHRLLDPTLATTGGAAGAPKGQPARADGAAEPGPMP
RVTDLLGDEGLIEPAPATGEEPRDLTREPLSFPADRPLRLQNLARGDEGFLLSLGYSTQRGYARSHPFVGEIRFGTVEVE
LFVEELGFAVPLGDIALTECQMVNQFKGSASEPPKFTRGYGLVFGQSERKAMSVALVDRALRFKELGEEQSAPAQDEEFV
LSHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRAEHADGTETSQQEAAE

Sequences:

>Translated_371_residues
MYVAVKGGETAIRNAHALLAETRRGDTSVPEIGIDQIAGQLTLAVDRVMSEGSLYDRPLAALAIKQARGDLIEAIFLVRA
FRTTLPRFGYSEPVDTGRMRVERRISATYKDIPGGQVLGPTFDYTHRLLDPTLATTGGAAGAPKGQPARADGAAEPGPMP
RVTDLLGDEGLIEPAPATGEEPRDLTREPLSFPADRPLRLQNLARGDEGFLLSLGYSTQRGYARSHPFVGEIRFGTVEVE
LFVEELGFAVPLGDIALTECQMVNQFKGSASEPPKFTRGYGLVFGQSERKAMSVALVDRALRFKELGEEQSAPAQDEEFV
LSHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRAEHADGTETSQQEAAE
>Mature_371_residues
MYVAVKGGETAIRNAHALLAETRRGDTSVPEIGIDQIAGQLTLAVDRVMSEGSLYDRPLAALAIKQARGDLIEAIFLVRA
FRTTLPRFGYSEPVDTGRMRVERRISATYKDIPGGQVLGPTFDYTHRLLDPTLATTGGAAGAPKGQPARADGAAEPGPMP
RVTDLLGDEGLIEPAPATGEEPRDLTREPLSFPADRPLRLQNLARGDEGFLLSLGYSTQRGYARSHPFVGEIRFGTVEVE
LFVEELGFAVPLGDIALTECQMVNQFKGSASEPPKFTRGYGLVFGQSERKAMSVALVDRALRFKELGEEQSAPAQDEEFV
LSHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRAEHADGTETSQQEAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=361, Percent_Identity=60.9418282548476, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 40407; Mature: 40407

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGETAIRNAHALLAETRRGDTSVPEIGIDQIAGQLTLAVDRVMSEGSLYDRPLA
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
ALAIKQARGDLIEAIFLVRAFRTTLPRFGYSEPVDTGRMRVERRISATYKDIPGGQVLGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECC
TFDYTHRLLDPTLATTGGAAGAPKGQPARADGAAEPGPMPRVTDLLGDEGLIEPAPATGE
CHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
EPRDLTREPLSFPADRPLRLQNLARGDEGFLLSLGYSTQRGYARSHPFVGEIRFGTVEVE
CCHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECEEEEE
LFVEELGFAVPLGDIALTECQMVNQFKGSASEPPKFTRGYGLVFGQSERKAMSVALVDRA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
LRFKELGEEQSAPAQDEEFVLSHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRAEHAD
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEECHHHHHHHHHHHHHHCCC
GTETSQQEAAE
CCCCCHHHCCC
>Mature Secondary Structure
MYVAVKGGETAIRNAHALLAETRRGDTSVPEIGIDQIAGQLTLAVDRVMSEGSLYDRPLA
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
ALAIKQARGDLIEAIFLVRAFRTTLPRFGYSEPVDTGRMRVERRISATYKDIPGGQVLGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECC
TFDYTHRLLDPTLATTGGAAGAPKGQPARADGAAEPGPMPRVTDLLGDEGLIEPAPATGE
CHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
EPRDLTREPLSFPADRPLRLQNLARGDEGFLLSLGYSTQRGYARSHPFVGEIRFGTVEVE
CCHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECEEEEE
LFVEELGFAVPLGDIALTECQMVNQFKGSASEPPKFTRGYGLVFGQSERKAMSVALVDRA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
LRFKELGEEQSAPAQDEEFVLSHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRAEHAD
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEECHHHHHHHHHHHHHHCCC
GTETSQQEAAE
CCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]