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The map label for this gene is phnH [H]

Identifier: 158425017

GI number: 158425017

Start: 3923879

End: 3924472

Strand: Direct

Name: phnH [H]

Synonym: AZC_3393

Alternate gene names: 158425017

Gene position: 3923879-3924472 (Clockwise)

Preceding gene: 158425016

Following gene: 158425018

Centisome position: 73.07

GC content: 70.2

Gene sequence:

>594_bases
ATGACCGATCTTGCCCTCGGCTTCGCCGATCCCGTCCGCGAGGCGCAGGCCACCTTCAAGGCGGTGATGTGGGCCATCTC
GCGCCCCGGCAACCGGATGCCGCTCGCCACCGGCCTCAAGCCTCCGGCGCCGCTCACGCCCGAAATGGCCGCCTGCGTCC
TCGCGGTCGCCGATTATGAGACGCCTCTCTGGCTCGATGCGAAGCTCGCCGCCCGCGCCGAAGTGCTGGACTTCCTGCGC
TTCCATACCGGGGCCCCGATCCTCGCGGAGCCGGGCGAGGCGCGCCTCGCGCTCATCAGCGATCCGGCGGCGCTCCCGCC
CCTCACCGATTTCGCCCAGGGCACGCCTGAGTATCCGGATCGCTCCACCACGCTCATTCTCGCGGTGGACGATCTCACCG
ATGCGCCCTTCACGCTGGAAGGGCCGGGAATCAAGCATACGCGCCGCTTCGGCGCGGCGCCGCTGCCCGCGGATTTTGCC
GCCCGCTGGGCCGACAACCGGCTGCTGTTTCCGCTGGGTGTCGATCTCATCTTCACCACGTCAGGGCTGGTGTCGGCCCT
GCCGCGCTCCACCCGCATCACCGCGGAGGCCTGA

Upstream 100 bases:

>100_bases
GCCCCGTGCGGGCCCGCCTCGCGGCGGAGGCAACGGACGCCCGAGAAAAGACAGAAGCGACCAAGGTGAACTTCTTCACC
ATGGTCCGTGGAGAGGACTG

Downstream 100 bases:

>100_bases
TCCATGTATGTGGCCGTCAAGGGTGGCGAGACCGCCATCCGCAACGCCCATGCCCTGCTCGCCGAGACCCGGCGTGGGGA
TACGTCGGTGCCGGAGATCG

Product: phosphonate metabolism protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MTDLALGFADPVREAQATFKAVMWAISRPGNRMPLATGLKPPAPLTPEMAACVLAVADYETPLWLDAKLAARAEVLDFLR
FHTGAPILAEPGEARLALISDPAALPPLTDFAQGTPEYPDRSTTLILAVDDLTDAPFTLEGPGIKHTRRFGAAPLPADFA
ARWADNRLLFPLGVDLIFTTSGLVSALPRSTRITAEA

Sequences:

>Translated_197_residues
MTDLALGFADPVREAQATFKAVMWAISRPGNRMPLATGLKPPAPLTPEMAACVLAVADYETPLWLDAKLAARAEVLDFLR
FHTGAPILAEPGEARLALISDPAALPPLTDFAQGTPEYPDRSTTLILAVDDLTDAPFTLEGPGIKHTRRFGAAPLPADFA
ARWADNRLLFPLGVDLIFTTSGLVSALPRSTRITAEA
>Mature_196_residues
TDLALGFADPVREAQATFKAVMWAISRPGNRMPLATGLKPPAPLTPEMAACVLAVADYETPLWLDAKLAARAEVLDFLRF
HTGAPILAEPGEARLALISDPAALPPLTDFAQGTPEYPDRSTTLILAVDDLTDAPFTLEGPGIKHTRRFGAAPLPADFAA
RWADNRLLFPLGVDLIFTTSGLVSALPRSTRITAEA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3625

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnH family [H]

Homologues:

Organism=Escherichia coli, GI1790538, Length=191, Percent_Identity=32.9842931937173, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008772 [H]

Pfam domain/function: PF05845 PhnH [H]

EC number: NA

Molecular weight: Translated: 21043; Mature: 20912

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLALGFADPVREAQATFKAVMWAISRPGNRMPLATGLKPPAPLTPEMAACVLAVADYE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
TPLWLDAKLAARAEVLDFLRFHTGAPILAEPGEARLALISDPAALPPLTDFAQGTPEYPD
CCEEECHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHCCCCCCCC
RSTTLILAVDDLTDAPFTLEGPGIKHTRRFGAAPLPADFAARWADNRLLFPLGVDLIFTT
CCCEEEEEECCCCCCCEEECCCCCCHHHHCCCCCCCHHHHHHHCCCEEEEEECCEEEEEC
SGLVSALPRSTRITAEA
CHHHHHCCCCCEEEECC
>Mature Secondary Structure 
TDLALGFADPVREAQATFKAVMWAISRPGNRMPLATGLKPPAPLTPEMAACVLAVADYE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
TPLWLDAKLAARAEVLDFLRFHTGAPILAEPGEARLALISDPAALPPLTDFAQGTPEYPD
CCEEECHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHCCCCCCCC
RSTTLILAVDDLTDAPFTLEGPGIKHTRRFGAAPLPADFAARWADNRLLFPLGVDLIFTT
CCCEEEEEECCCCCCCEEECCCCCCHHHHCCCCCCCHHHHHHHCCCEEEEEECCEEEEEC
SGLVSALPRSTRITAEA
CHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]