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The map label for this gene is fadD25 [H]

Identifier: 158424991

GI number: 158424991

Start: 3896316

End: 3898061

Strand: Direct

Name: fadD25 [H]

Synonym: AZC_3367

Alternate gene names: 158424991

Gene position: 3896316-3898061 (Clockwise)

Preceding gene: 158424985

Following gene: 158424992

Centisome position: 72.56

GC content: 68.79

Gene sequence:

>1746_bases
ATGAGCTCCTTCTCCTCGCTGGTGGACATGCTGCGCCATCAGGCCCAGCACACGCCCGACAAGCGCGCCCTCGTCTTCTC
TTCCGGCAAGCCGGAGATCGAGCCGGAACTAAGCTTCGCCGGCCTCTACGCGCAGGCCGCGACGCTGGCGCGGCAGATTG
CGGATCAGACGCAGCCCGGCGACCGGGCGCTGCTCGTCTTCCCGTCCGGGCTTGAGTTCGTCGTCTCCTATTTCGCCTGC
CTCATGGCGGGCGTGGTGGCGGTGCCGCTCATGCCGCCCCGGCGCAACGCAAATCAGGATTCCAGCGCCGCCATCATCGC
AGATTGCGCCCCCGTGCTCGCGCTCAGCCCGGCGGGCGAGGCGGAAGACCCGCGCGGCACGCTGCGCCAGCGCCTCGCGG
ACGGCAATGTCCGGCATTTGCCGGTCGCGCTCGCGGACAATGCGACCGACGATCTGCCCGCTCGCGCCATCGCCCGGCAG
GATCTGGCCTTCCTGCAATATACGTCCGGCTCCACCTCCACCCCCAAGGGCGTGATGGTGAGCCACGCCAACCTGCTGGC
CAATCTGGAAATGATGCGCCAGCGCTTCGGCAATCACGCGGGTTCGACCCATGTGAGCTGGATCCCGCTCTATCACGACC
TCGGGCTGATCCTGAACCTGCTGGAGCCGCTGTATGTGGGCGCGACGTGCGTGCTCATGACCCCCTCCGGCTTCATGCAC
CGACCGCTGAACTGGCTGCGCAACATTGCGCGCTTCGAGGCCGAAGTCGCTGCCGCGCCCAACTTTGCCTACCACCTCTG
CGTCGACCGCTTCCGGGCCGACCAGATGGAGGGCGTGGACCTCAGCCGCTGGACGCTGGCGGTGAACGGTGCCGAGCCGG
TACGCGCCGTGACCATGGCGCGCTTTGCCGAGACCTTCGCCCCTTACGGCTTCAACGCCCGCACGCTCCATCCCACCTAC
GGGCTGGCGGAAGCGACCCTCGTCGTGAGTTCCGCCCCGCGCGGCAGCGGGGTAACGGTGCGCGAGGTGAGCGCCGAAGG
GCTGAAGGACCGTCGCATCGCGCCGCCGAAGGACGCGGAAGACCGCCAGCCCATCGTCGGCTGCGGCCTCACCATGCCGG
GCCAGAGCATCGCCATCGTCGATCCGCAGACCCGCCGCCGCGCCGAGCCCGGTGCCATCGGCGAGATCTGGGTGCATGGG
GCGAGCGTCGCCGGCGGCTACTGGAACCGGCCGGAGGAGAGCGAGGCGACCTTCCGGGCGCACATCGAGGACGAGCCCGA
CGCCGGCCCCTTCCTGCGCACCGGCGACCTCGGACATCTGGATGCGGATGGCGAACTCTTCATCGCCGGCCGCATCAAGG
ACGTCATCATCATCCGCGGCGTGAACCATTATCCGCAGGACATCGAGGGCACGGTGGCGGATGCCCACCCCACCCTGCGC
CGGGACTATGGCGCGGTGTTCTGCGTCACCGGCGAGGACGGCACCGAGCAGGTGGTGCTGGTGCAGGAGGTGGAACGCAC
CAAGCGCCATGCCATCGACGAGACGGAAGTCTTCGCCGCGATCCGGGCGGCGGTGGTCAACAATCACGAGGTGAACGTGG
CGAAGATCGTGCTGATCCGCCCCGGCACCATCCCCAAGACCACCAGCGGCAAGATCCAGCGCAGCCGCACCCGCCAGCAG
TGGCTGGACGGCACGCTGGAGGTGTGGGATCCCCGCGCACCGGAATCGGCGGCCGAAGCCGTCTGA

Upstream 100 bases:

>100_bases
TCGATGCCGGTTCGCGGCACCAGCGGAAGCTTGACACCGTGCGTTCCCCGTCCTCTCTCGCCGCGCGGGCACCGATGGCC
GGCGGTTTCAGATCCGAATC

Downstream 100 bases:

>100_bases
CACTTTCGAACAGAGCCCGCATCATGCCCAGCCCCAGCGACATCCTCAGCCATTGCCGCGAGCGCCTCGCGGCCATCGTG
GAGACGGACGCCTCAGCCAT

Product: AMP-dependent synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MSSFSSLVDMLRHQAQHTPDKRALVFSSGKPEIEPELSFAGLYAQAATLARQIADQTQPGDRALLVFPSGLEFVVSYFAC
LMAGVVAVPLMPPRRNANQDSSAAIIADCAPVLALSPAGEAEDPRGTLRQRLADGNVRHLPVALADNATDDLPARAIARQ
DLAFLQYTSGSTSTPKGVMVSHANLLANLEMMRQRFGNHAGSTHVSWIPLYHDLGLILNLLEPLYVGATCVLMTPSGFMH
RPLNWLRNIARFEAEVAAAPNFAYHLCVDRFRADQMEGVDLSRWTLAVNGAEPVRAVTMARFAETFAPYGFNARTLHPTY
GLAEATLVVSSAPRGSGVTVREVSAEGLKDRRIAPPKDAEDRQPIVGCGLTMPGQSIAIVDPQTRRRAEPGAIGEIWVHG
ASVAGGYWNRPEESEATFRAHIEDEPDAGPFLRTGDLGHLDADGELFIAGRIKDVIIIRGVNHYPQDIEGTVADAHPTLR
RDYGAVFCVTGEDGTEQVVLVQEVERTKRHAIDETEVFAAIRAAVVNNHEVNVAKIVLIRPGTIPKTTSGKIQRSRTRQQ
WLDGTLEVWDPRAPESAAEAV

Sequences:

>Translated_581_residues
MSSFSSLVDMLRHQAQHTPDKRALVFSSGKPEIEPELSFAGLYAQAATLARQIADQTQPGDRALLVFPSGLEFVVSYFAC
LMAGVVAVPLMPPRRNANQDSSAAIIADCAPVLALSPAGEAEDPRGTLRQRLADGNVRHLPVALADNATDDLPARAIARQ
DLAFLQYTSGSTSTPKGVMVSHANLLANLEMMRQRFGNHAGSTHVSWIPLYHDLGLILNLLEPLYVGATCVLMTPSGFMH
RPLNWLRNIARFEAEVAAAPNFAYHLCVDRFRADQMEGVDLSRWTLAVNGAEPVRAVTMARFAETFAPYGFNARTLHPTY
GLAEATLVVSSAPRGSGVTVREVSAEGLKDRRIAPPKDAEDRQPIVGCGLTMPGQSIAIVDPQTRRRAEPGAIGEIWVHG
ASVAGGYWNRPEESEATFRAHIEDEPDAGPFLRTGDLGHLDADGELFIAGRIKDVIIIRGVNHYPQDIEGTVADAHPTLR
RDYGAVFCVTGEDGTEQVVLVQEVERTKRHAIDETEVFAAIRAAVVNNHEVNVAKIVLIRPGTIPKTTSGKIQRSRTRQQ
WLDGTLEVWDPRAPESAAEAV
>Mature_580_residues
SSFSSLVDMLRHQAQHTPDKRALVFSSGKPEIEPELSFAGLYAQAATLARQIADQTQPGDRALLVFPSGLEFVVSYFACL
MAGVVAVPLMPPRRNANQDSSAAIIADCAPVLALSPAGEAEDPRGTLRQRLADGNVRHLPVALADNATDDLPARAIARQD
LAFLQYTSGSTSTPKGVMVSHANLLANLEMMRQRFGNHAGSTHVSWIPLYHDLGLILNLLEPLYVGATCVLMTPSGFMHR
PLNWLRNIARFEAEVAAAPNFAYHLCVDRFRADQMEGVDLSRWTLAVNGAEPVRAVTMARFAETFAPYGFNARTLHPTYG
LAEATLVVSSAPRGSGVTVREVSAEGLKDRRIAPPKDAEDRQPIVGCGLTMPGQSIAIVDPQTRRRAEPGAIGEIWVHGA
SVAGGYWNRPEESEATFRAHIEDEPDAGPFLRTGDLGHLDADGELFIAGRIKDVIIIRGVNHYPQDIEGTVADAHPTLRR
DYGAVFCVTGEDGTEQVVLVQEVERTKRHAIDETEVFAAIRAAVVNNHEVNVAKIVLIRPGTIPKTTSGKIQRSRTRQQW
LDGTLEVWDPRAPESAAEAV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=584, Percent_Identity=25.8561643835616, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI225735625, Length=569, Percent_Identity=25.3075571177504, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI225735629, Length=569, Percent_Identity=25.3075571177504, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI45827692, Length=569, Percent_Identity=25.3075571177504, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI55749758, Length=573, Percent_Identity=23.9092495636998, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI45827694, Length=506, Percent_Identity=24.703557312253, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI225735627, Length=478, Percent_Identity=24.0585774058577, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI45827696, Length=478, Percent_Identity=24.0585774058577, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI45827698, Length=441, Percent_Identity=24.2630385487528, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI27477105, Length=332, Percent_Identity=25.6024096385542, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788107, Length=601, Percent_Identity=27.6206322795341, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI193202679, Length=605, Percent_Identity=23.9669421487603, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=450, Percent_Identity=26, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=419, Percent_Identity=25.0596658711217, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18859661, Length=472, Percent_Identity=23.9406779661017, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654656, Length=464, Percent_Identity=22.4137931034483, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581924, Length=554, Percent_Identity=25.9927797833935, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281365686, Length=106, Percent_Identity=33.0188679245283, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648260, Length=195, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63137; Mature: 63006

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFSSLVDMLRHQAQHTPDKRALVFSSGKPEIEPELSFAGLYAQAATLARQIADQTQPG
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
DRALLVFPSGLEFVVSYFACLMAGVVAVPLMPPRRNANQDSSAAIIADCAPVLALSPAGE
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEECCCCC
AEDPRGTLRQRLADGNVRHLPVALADNATDDLPARAIARQDLAFLQYTSGSTSTPKGVMV
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCEEE
SHANLLANLEMMRQRFGNHAGSTHVSWIPLYHDLGLILNLLEPLYVGATCVLMTPSGFMH
EHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
RPLNWLRNIARFEAEVAAAPNFAYHLCVDRFRADQMEGVDLSRWTLAVNGAEPVRAVTMA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHH
RFAETFAPYGFNARTLHPTYGLAEATLVVSSAPRGSGVTVREVSAEGLKDRRIAPPKDAE
HHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCC
DRQPIVGCGLTMPGQSIAIVDPQTRRRAEPGAIGEIWVHGASVAGGYWNRPEESEATFRA
CCCCEEEECCCCCCCEEEEECCHHHCCCCCCCCEEEEEECCEECCCCCCCCCCCCCEEEE
HIEDEPDAGPFLRTGDLGHLDADGELFIAGRIKDVIIIRGVNHYPQDIEGTVADAHPTLR
ECCCCCCCCCCEECCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHH
RDYGAVFCVTGEDGTEQVVLVQEVERTKRHAIDETEVFAAIRAAVVNNHEVNVAKIVLIR
CCCCEEEEEECCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEC
PGTIPKTTSGKIQRSRTRQQWLDGTLEVWDPRAPESAAEAV
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHCCC
>Mature Secondary Structure 
SSFSSLVDMLRHQAQHTPDKRALVFSSGKPEIEPELSFAGLYAQAATLARQIADQTQPG
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
DRALLVFPSGLEFVVSYFACLMAGVVAVPLMPPRRNANQDSSAAIIADCAPVLALSPAGE
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEECCCCC
AEDPRGTLRQRLADGNVRHLPVALADNATDDLPARAIARQDLAFLQYTSGSTSTPKGVMV
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCEEE
SHANLLANLEMMRQRFGNHAGSTHVSWIPLYHDLGLILNLLEPLYVGATCVLMTPSGFMH
EHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
RPLNWLRNIARFEAEVAAAPNFAYHLCVDRFRADQMEGVDLSRWTLAVNGAEPVRAVTMA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHH
RFAETFAPYGFNARTLHPTYGLAEATLVVSSAPRGSGVTVREVSAEGLKDRRIAPPKDAE
HHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCC
DRQPIVGCGLTMPGQSIAIVDPQTRRRAEPGAIGEIWVHGASVAGGYWNRPEESEATFRA
CCCCEEEECCCCCCCEEEEECCHHHCCCCCCCCEEEEEECCEECCCCCCCCCCCCCEEEE
HIEDEPDAGPFLRTGDLGHLDADGELFIAGRIKDVIIIRGVNHYPQDIEGTVADAHPTLR
ECCCCCCCCCCEECCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHH
RDYGAVFCVTGEDGTEQVVLVQEVERTKRHAIDETEVFAAIRAAVVNNHEVNVAKIVLIR
CCCCEEEEEECCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEC
PGTIPKTTSGKIQRSRTRQQWLDGTLEVWDPRAPESAAEAV
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]