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The map label for this gene is garD [H]

Identifier: 158424964

GI number: 158424964

Start: 3866536

End: 3868098

Strand: Reverse

Name: garD [H]

Synonym: AZC_3340

Alternate gene names: 158424964

Gene position: 3868098-3866536 (Counterclockwise)

Preceding gene: 158424974

Following gene: 158424962

Centisome position: 72.03

GC content: 66.6

Gene sequence:

>1563_bases
ATGGATGAGCTGGTTCGCGAAGGGGCTTCGGAGGTGCGCGCCGGCGCAGCTTCGCCGCGCGTTCTGCATCTGGCGGCCTC
CGACAATGTAGCTGTGGCGCTGGATGCGCTCGCGCCGGGTGTTGTGGTGTCCGGCATCACGGCGCAGGCGCGCATTCCGC
GCGGCCACAAGTTTGCGCTGGTGCCCATCGCCGAGGGCGAGCCGGTGCGCAAGCTGGGCCAGATCATCGGCTTTGCCGCG
CGGGCCATCGCGCCGGGTGAGCATGTGCACGAGCACAATGTGGTGCTGCACGATTTCTCCCGCGACTACGCCTTCGCGGT
GGACGCCCACGACGACGGTCTGTTGCCGGAGGCCGAGCGGGCGACCTTTCAGGGCTATCGCCGCTCCAACGGCCGCACGG
GCACGCGCAATTATCTCGGCATTCTCACCAGCGTGAACTGCTCGGCCTCGGTGGCGCGCTTCGCGGCGCAGGAACTGGAG
CGCTCAGGGTTTCTGGAGCAATTTCCAGGCATCGACGGCATCGTACCCATCACCCACGGTACCGGCTGCGGCATGGCCTC
CAAGGGCGAGGGATTCGACATCCTGAACCGCACCGTGTGGGGCTATGCGACGCATCCCAATTTCGCTGCCGTGGTGATGG
TGGGACTGGGCTGCGAGGTGTTCCAGATCGCCCGCTTCAAGTCCGAATATGGTGTGCAGGAGAGCGACGGCTTCCGCTCG
CTGACCATTCAGGACGAGGGCGGCACCCGCAAGACGGTGGAGCATCTGGTTGGCGCGCTCAAGGAGATGCTGCCTGCCGC
CGCCGCGCACCGACGGCAGACGCGCCCGGCGGCTGAGCTCATCCTCGCCCTCCAGTGCGGCGGCTCGGACGGCTACAGCG
CGCTCACCGCCAATCCGGCGCTCGGTGTCGCCTCCGATCTTCTCGTGCGCAATGGCGGCACGTCCATCCTCTCCGAGACG
CCGGAAATCTACGGCGCCGAGCACCTGCTCACCCGCCGCGCAGCGAACCGCGCCGTGGGCGAGAAGCTGGTGGCGCGCAT
CAAATGGTGGGAGGAATACACCTCGCGCAGCGCGGGCGAGATGAACAACAATCCCTCGCCCGGCAATAAGGCGGGCGGTC
TCACCACCATTCTGGAGAAGTCGCTGGGCGCGGCGGCAAAAGGCGGCGCCTCGACGCTCCGGGCCGTCTATGAATATGCC
GAGCCGGTGACGGAGCATGGCTTCGTCTATATGGACACCCCCGGCTATGATCCCGTGGCTGCCACGGGCCAGGTGGCGGG
CGGGGCCAATCTCATCTGTTTCACCACCGGGCGTGGCTCCGCCTTCGGCTGCAAGCCCACGCCGTCGCTCAAGCTCGCCA
CCAATTCCGACATCTACCGCCGGATGGAAGAGGACATGGACATCAATTGCGGCGATGTCCTCGACGGCGTGACGCTGGAA
GAGAAGGGGCGCGAGATCTTCGACAAGATCCTGCGCACCGCTTCCGGCGAGCGCACGAAGTCCGAGGCGCTCGGCTATGG
CGATGCGGAATTCGTGCCCTGGCAGGTGGGCGCTGTGATGTGA

Upstream 100 bases:

>100_bases
CTCCGCTGGCACGTTTCCTCGCTTCCACTTGTGATGTTTTGCGATGCTAATATATTAGCATATCAACGCCGTCAACGATA
ACGCTGCGGGAGGTAGACCA

Downstream 100 bases:

>100_bases
GGCGTTCCCGGCCTATTCGAACAGGTCGGGATGCTCCTGCGCGACCTTGGGCAAGGCGCGCAGGATCTCGCTCAGGTGAC
AGCGCATGGCTTCCGCCGCG

Product: altronate hydrolase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MDELVREGASEVRAGAASPRVLHLAASDNVAVALDALAPGVVVSGITAQARIPRGHKFALVPIAEGEPVRKLGQIIGFAA
RAIAPGEHVHEHNVVLHDFSRDYAFAVDAHDDGLLPEAERATFQGYRRSNGRTGTRNYLGILTSVNCSASVARFAAQELE
RSGFLEQFPGIDGIVPITHGTGCGMASKGEGFDILNRTVWGYATHPNFAAVVMVGLGCEVFQIARFKSEYGVQESDGFRS
LTIQDEGGTRKTVEHLVGALKEMLPAAAAHRRQTRPAAELILALQCGGSDGYSALTANPALGVASDLLVRNGGTSILSET
PEIYGAEHLLTRRAANRAVGEKLVARIKWWEEYTSRSAGEMNNNPSPGNKAGGLTTILEKSLGAAAKGGASTLRAVYEYA
EPVTEHGFVYMDTPGYDPVAATGQVAGGANLICFTTGRGSAFGCKPTPSLKLATNSDIYRRMEEDMDINCGDVLDGVTLE
EKGREIFDKILRTASGERTKSEALGYGDAEFVPWQVGAVM

Sequences:

>Translated_520_residues
MDELVREGASEVRAGAASPRVLHLAASDNVAVALDALAPGVVVSGITAQARIPRGHKFALVPIAEGEPVRKLGQIIGFAA
RAIAPGEHVHEHNVVLHDFSRDYAFAVDAHDDGLLPEAERATFQGYRRSNGRTGTRNYLGILTSVNCSASVARFAAQELE
RSGFLEQFPGIDGIVPITHGTGCGMASKGEGFDILNRTVWGYATHPNFAAVVMVGLGCEVFQIARFKSEYGVQESDGFRS
LTIQDEGGTRKTVEHLVGALKEMLPAAAAHRRQTRPAAELILALQCGGSDGYSALTANPALGVASDLLVRNGGTSILSET
PEIYGAEHLLTRRAANRAVGEKLVARIKWWEEYTSRSAGEMNNNPSPGNKAGGLTTILEKSLGAAAKGGASTLRAVYEYA
EPVTEHGFVYMDTPGYDPVAATGQVAGGANLICFTTGRGSAFGCKPTPSLKLATNSDIYRRMEEDMDINCGDVLDGVTLE
EKGREIFDKILRTASGERTKSEALGYGDAEFVPWQVGAVM
>Mature_520_residues
MDELVREGASEVRAGAASPRVLHLAASDNVAVALDALAPGVVVSGITAQARIPRGHKFALVPIAEGEPVRKLGQIIGFAA
RAIAPGEHVHEHNVVLHDFSRDYAFAVDAHDDGLLPEAERATFQGYRRSNGRTGTRNYLGILTSVNCSASVARFAAQELE
RSGFLEQFPGIDGIVPITHGTGCGMASKGEGFDILNRTVWGYATHPNFAAVVMVGLGCEVFQIARFKSEYGVQESDGFRS
LTIQDEGGTRKTVEHLVGALKEMLPAAAAHRRQTRPAAELILALQCGGSDGYSALTANPALGVASDLLVRNGGTSILSET
PEIYGAEHLLTRRAANRAVGEKLVARIKWWEEYTSRSAGEMNNNPSPGNKAGGLTTILEKSLGAAAKGGASTLRAVYEYA
EPVTEHGFVYMDTPGYDPVAATGQVAGGANLICFTTGRGSAFGCKPTPSLKLATNSDIYRRMEEDMDINCGDVLDGVTLE
EKGREIFDKILRTASGERTKSEALGYGDAEFVPWQVGAVM

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=499, Percent_Identity=38.877755511022, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1789475, Length=491, Percent_Identity=36.2525458248472, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 55263; Mature: 55263

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELVREGASEVRAGAASPRVLHLAASDNVAVALDALAPGVVVSGITAQARIPRGHKFAL
CCHHHHHCHHHHHCCCCCCEEEEEECCCCEEEEEEHHCCCCEEECCEEEEECCCCCEEEE
VPIAEGEPVRKLGQIIGFAARAIAPGEHVHEHNVVLHDFSRDYAFAVDAHDDGLLPEAER
EEECCCCHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHH
ATFQGYRRSNGRTGTRNYLGILTSVNCSASVARFAAQELERSGFLEQFPGIDGIVPITHG
HHHHHHHHCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECC
TGCGMASKGEGFDILNRTVWGYATHPNFAAVVMVGLGCEVFQIARFKSEYGVQESDGFRS
CCCCCCCCCCCCHHHHCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEE
LTIQDEGGTRKTVEHLVGALKEMLPAAAAHRRQTRPAAELILALQCGGSDGYSALTANPA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCEEECCCC
LGVASDLLVRNGGTSILSETPEIYGAEHLLTRRAANRAVGEKLVARIKWWEEYTSRSAGE
CCHHHHHHEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MNNNPSPGNKAGGLTTILEKSLGAAAKGGASTLRAVYEYAEPVTEHGFVYMDTPGYDPVA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
ATGQVAGGANLICFTTGRGSAFGCKPTPSLKLATNSDIYRRMEEDMDINCGDVLDGVTLE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHCCCCHH
EKGREIFDKILRTASGERTKSEALGYGDAEFVPWQVGAVM
HHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MDELVREGASEVRAGAASPRVLHLAASDNVAVALDALAPGVVVSGITAQARIPRGHKFAL
CCHHHHHCHHHHHCCCCCCEEEEEECCCCEEEEEEHHCCCCEEECCEEEEECCCCCEEEE
VPIAEGEPVRKLGQIIGFAARAIAPGEHVHEHNVVLHDFSRDYAFAVDAHDDGLLPEAER
EEECCCCHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHH
ATFQGYRRSNGRTGTRNYLGILTSVNCSASVARFAAQELERSGFLEQFPGIDGIVPITHG
HHHHHHHHCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECC
TGCGMASKGEGFDILNRTVWGYATHPNFAAVVMVGLGCEVFQIARFKSEYGVQESDGFRS
CCCCCCCCCCCCHHHHCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEE
LTIQDEGGTRKTVEHLVGALKEMLPAAAAHRRQTRPAAELILALQCGGSDGYSALTANPA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCEEECCCC
LGVASDLLVRNGGTSILSETPEIYGAEHLLTRRAANRAVGEKLVARIKWWEEYTSRSAGE
CCHHHHHHEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MNNNPSPGNKAGGLTTILEKSLGAAAKGGASTLRAVYEYAEPVTEHGFVYMDTPGYDPVA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
ATGQVAGGANLICFTTGRGSAFGCKPTPSLKLATNSDIYRRMEEDMDINCGDVLDGVTLE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHCCCCHH
EKGREIFDKILRTASGERTKSEALGYGDAEFVPWQVGAVM
HHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]