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The map label for this gene is garD [H]

Identifier: 158424902

GI number: 158424902

Start: 3784959

End: 3786482

Strand: Reverse

Name: garD [H]

Synonym: AZC_3278

Alternate gene names: 158424902

Gene position: 3786482-3784959 (Counterclockwise)

Preceding gene: 158424903

Following gene: 158424901

Centisome position: 70.51

GC content: 66.4

Gene sequence:

>1524_bases
ATGTCCAGCCCGCGCACGCTGCGTCTCCACGAGACGGACAATGTCGTCATCGTCCTCGATACCGTTGCTCGGGGCGCGGC
GCTGGCTGGCGGCCTGACGGCGGCGGGGCCCGTGCCCCGCGGCCACAAGATCGCGGCCGCCCCCATTCCCGCCGGGGCGC
CGGTGCGCAAGTTCGGGCAGGTCATCGCCTTCGCCAGGACGGACATCGCGCCGGGCGAATGGGTGCATGAACACAATGTG
ATGCTGCAGGACTTCGAGCGCGATTATGCCTTCGCCTCGGAGGCCCGCGCGCCCGAAGGCCTGCTGCCGGGCGAGATGCC
CGCCACCTTCGAGGGCTTCCGCCGGGCCGATGGCCGCGCGGGCACGCGCAACTACATCGGCATCCTCAGCTCGGTGAACT
GCTCGGCCACCGTGGCGGACTACATTGCGGATGAGGTGACGCGCTCCGGCATCCTCGCGGATTATCCCAACATCGACGGC
GTGGTGCCGTTCACCCACGGCACCGGCTGCGGCATGGGCAGCAAGGGGGAGGAGTTCGACATCCTCGCCCGCACCCAGTG
GGGCTATGCCACCCATCCCAATTTTGCGGCCGTGCTGGTTATCGGCCTTGGTTGCGAGGTGTTCCAGATCCCGCGTCTCA
AGGAGGCCTATGGCATCGTTGAGGGGGAGCATTTCCAGAGTCTGACGATCCAGGAGAGCGGCGGCACTCGAAAGTCCGTG
GCGGCCGGCGTCGCGCGGGTGAAGGAGATGCTGGCCTTCGCCAATTCATCCCGGCGGCAGACGGTGGCGGCCTCCGAGCT
GATGCTGGCGCTCCAATGCGGCGGCTCGGACGGCTATTCCGGCCTCACCGCCAATCCCGCGCTGGGGGCGGCGGTGGATA
TTCTGGTGCGGCAGGGCGGCACGGCCATCCTTTCCGAGACGCCGGAAATCTTCGGTGCCGAGCATCTGCTTACCCGCAGG
GCCGAGAGCGAGGCGGTGGGTCGCAAGATCGTCGATCTCATCGACTGGTGGACGGACTATGCCGCCCGCGCCGGTGGCGA
GCTGAACAACAATCCGTCCCCCGGCAACAAGGCCGGCGGCCTCACCACCATTCTTGAAAAGTCATTGGGTGCGGTGGCGA
AGGGCGGCACTTCCACCCTGCGCGGCGTCTATCGCTATGCCGAGCGGATCGACCGCAAGGGCTTCGTTTATATGGACACG
CCCGGCTACGATCCGGTGGCCGCCACCGGGCAGGTGGCGGGTGGGGCGAACGTGCTGTGCTTCACTACCGGCCGTGGCTC
GGCCTATGGCTGTAAGCCCACGCCCTCCATCAAGCTGGCCACCAACAGCGATCTCTATCGCCGGATGGAAGAAGACATGG
ACCTCAACTGCGGTGATGTCCTTGAAGGCGTCAGCATTGAGGAGAAGGGCCGGGAGATCTTCCAGACGGTGCTCGATGTC
GCTTCCGGCACTCGCTCCAAGTCCGAACTGCTCGGCTATGGCCGAAACGAATTCGTGCCCTGGCAGCTCGGCGCGGTGAT
GTGA

Upstream 100 bases:

>100_bases
CCATCGCGGCGCGCTCTCCGACGCGCCCGCTTTGTTCCAGGAGTGGCTGCGGCCGGAGGCCGGGGTCATCAAGGCGATCC
TGGAAGTCTGAGGTTTCTTC

Downstream 100 bases:

>100_bases
GCGAAGGGGCGCGCCCGCAGCAGGGTGCGCCTTCCGGTGAGGCTGGACGAAGCGTGGTCGCCGTTCAGCGGTCTTGCATT
GATGAGCCGGGTTCACCGCT

Product: altronate hydrolase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSSPRTLRLHETDNVVIVLDTVARGAALAGGLTAAGPVPRGHKIAAAPIPAGAPVRKFGQVIAFARTDIAPGEWVHEHNV
MLQDFERDYAFASEARAPEGLLPGEMPATFEGFRRADGRAGTRNYIGILSSVNCSATVADYIADEVTRSGILADYPNIDG
VVPFTHGTGCGMGSKGEEFDILARTQWGYATHPNFAAVLVIGLGCEVFQIPRLKEAYGIVEGEHFQSLTIQESGGTRKSV
AAGVARVKEMLAFANSSRRQTVAASELMLALQCGGSDGYSGLTANPALGAAVDILVRQGGTAILSETPEIFGAEHLLTRR
AESEAVGRKIVDLIDWWTDYAARAGGELNNNPSPGNKAGGLTTILEKSLGAVAKGGTSTLRGVYRYAERIDRKGFVYMDT
PGYDPVAATGQVAGGANVLCFTTGRGSAYGCKPTPSIKLATNSDLYRRMEEDMDLNCGDVLEGVSIEEKGREIFQTVLDV
ASGTRSKSELLGYGRNEFVPWQLGAVM

Sequences:

>Translated_507_residues
MSSPRTLRLHETDNVVIVLDTVARGAALAGGLTAAGPVPRGHKIAAAPIPAGAPVRKFGQVIAFARTDIAPGEWVHEHNV
MLQDFERDYAFASEARAPEGLLPGEMPATFEGFRRADGRAGTRNYIGILSSVNCSATVADYIADEVTRSGILADYPNIDG
VVPFTHGTGCGMGSKGEEFDILARTQWGYATHPNFAAVLVIGLGCEVFQIPRLKEAYGIVEGEHFQSLTIQESGGTRKSV
AAGVARVKEMLAFANSSRRQTVAASELMLALQCGGSDGYSGLTANPALGAAVDILVRQGGTAILSETPEIFGAEHLLTRR
AESEAVGRKIVDLIDWWTDYAARAGGELNNNPSPGNKAGGLTTILEKSLGAVAKGGTSTLRGVYRYAERIDRKGFVYMDT
PGYDPVAATGQVAGGANVLCFTTGRGSAYGCKPTPSIKLATNSDLYRRMEEDMDLNCGDVLEGVSIEEKGREIFQTVLDV
ASGTRSKSELLGYGRNEFVPWQLGAVM
>Mature_506_residues
SSPRTLRLHETDNVVIVLDTVARGAALAGGLTAAGPVPRGHKIAAAPIPAGAPVRKFGQVIAFARTDIAPGEWVHEHNVM
LQDFERDYAFASEARAPEGLLPGEMPATFEGFRRADGRAGTRNYIGILSSVNCSATVADYIADEVTRSGILADYPNIDGV
VPFTHGTGCGMGSKGEEFDILARTQWGYATHPNFAAVLVIGLGCEVFQIPRLKEAYGIVEGEHFQSLTIQESGGTRKSVA
AGVARVKEMLAFANSSRRQTVAASELMLALQCGGSDGYSGLTANPALGAAVDILVRQGGTAILSETPEIFGAEHLLTRRA
ESEAVGRKIVDLIDWWTDYAARAGGELNNNPSPGNKAGGLTTILEKSLGAVAKGGTSTLRGVYRYAERIDRKGFVYMDTP
GYDPVAATGQVAGGANVLCFTTGRGSAYGCKPTPSIKLATNSDLYRRMEEDMDLNCGDVLEGVSIEEKGREIFQTVLDVA
SGTRSKSELLGYGRNEFVPWQLGAVM

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=489, Percent_Identity=39.8773006134969, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI1789475, Length=499, Percent_Identity=34.8697394789579, Blast_Score=279, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 53782; Mature: 53651

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRTLRLHETDNVVIVLDTVARGAALAGGLTAAGPVPRGHKIAAAPIPAGAPVRKFGQ
CCCCCEEEEECCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
VIAFARTDIAPGEWVHEHNVMLQDFERDYAFASEARAPEGLLPGEMPATFEGFRRADGRA
HHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
GTRNYIGILSSVNCSATVADYIADEVTRSGILADYPNIDGVVPFTHGTGCGMGSKGEEFD
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCEEECCCCCCCCCCCCCCCEE
ILARTQWGYATHPNFAAVLVIGLGCEVFQIPRLKEAYGIVEGEHFQSLTIQESGGTRKSV
EEEEECCCCCCCCCCEEEEEEECCCCEEECCCHHHHHCCCCCCCCEEEEEECCCCCHHHH
AAGVARVKEMLAFANSSRRQTVAASELMLALQCGGSDGYSGLTANPALGAAVDILVRQGG
HHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
TAILSETPEIFGAEHLLTRRAESEAVGRKIVDLIDWWTDYAARAGGELNNNPSPGNKAGG
CEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LTTILEKSLGAVAKGGTSTLRGVYRYAERIDRKGFVYMDTPGYDPVAATGQVAGGANVLC
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEE
FTTGRGSAYGCKPTPSIKLATNSDLYRRMEEDMDLNCGDVLEGVSIEEKGREIFQTVLDV
EECCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
ASGTRSKSELLGYGRNEFVPWQLGAVM
HCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSPRTLRLHETDNVVIVLDTVARGAALAGGLTAAGPVPRGHKIAAAPIPAGAPVRKFGQ
CCCCEEEEECCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
VIAFARTDIAPGEWVHEHNVMLQDFERDYAFASEARAPEGLLPGEMPATFEGFRRADGRA
HHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
GTRNYIGILSSVNCSATVADYIADEVTRSGILADYPNIDGVVPFTHGTGCGMGSKGEEFD
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCEEECCCCCCCCCCCCCCCEE
ILARTQWGYATHPNFAAVLVIGLGCEVFQIPRLKEAYGIVEGEHFQSLTIQESGGTRKSV
EEEEECCCCCCCCCCEEEEEEECCCCEEECCCHHHHHCCCCCCCCEEEEEECCCCCHHHH
AAGVARVKEMLAFANSSRRQTVAASELMLALQCGGSDGYSGLTANPALGAAVDILVRQGG
HHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
TAILSETPEIFGAEHLLTRRAESEAVGRKIVDLIDWWTDYAARAGGELNNNPSPGNKAGG
CEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LTTILEKSLGAVAKGGTSTLRGVYRYAERIDRKGFVYMDTPGYDPVAATGQVAGGANVLC
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEE
FTTGRGSAYGCKPTPSIKLATNSDLYRRMEEDMDLNCGDVLEGVSIEEKGREIFQTVLDV
EECCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
ASGTRSKSELLGYGRNEFVPWQLGAVM
HCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]