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The map label for this gene is yxeK [H]

Identifier: 158424802

GI number: 158424802

Start: 3633820

End: 3635193

Strand: Direct

Name: yxeK [H]

Synonym: AZC_3178

Alternate gene names: 158424802

Gene position: 3633820-3635193 (Clockwise)

Preceding gene: 158424801

Following gene: 158424803

Centisome position: 67.67

GC content: 67.83

Gene sequence:

>1374_bases
ATGTCGCCCCGCCGTGAACTGCGCCTCAACACCTTCCAGATGGCCGCGCCCTCGCACAATTGGGCGGGGCTCTGGAGCCA
TCCGCGGGACAACCAGACGCAGTACAACACCCTCGCTTATTGGACCGAGATGGCGAAGACCGCCGAGCGCGGCCTGCTTG
ACGGCATTTTCATCGCGGACGTGTTCGGCCTCTATGACGTCTATGGCGGCTCCGGAGACGCCGCGATCGCCGCCGGCGCG
CAGGCGCCCAATGCCGACCCGACTCTCGCCATCTCCGCCATGGCGCTGGTGACGCAGCACATCGGCTTCGGCGTCACCGC
CAATCTCACCTATGAGCATCCCTTCCAGTTCGCCCGCCGCTTCACCACCCTCGACCACCTCACAGGCGGGAGGCTCGGCT
GGAACATCGTCACCGGCTATCTCGACAGCGGCGCGCGCGGCATGGGCGAGAGCCTGAACCGCGACCATGACGAGCGCTAC
GAGGCGGCCGAGGATTTCATGGCCGCCATCTACAAGCTCTGGGAAGGCTCGTGGGAGGATGACGCCGCCCTTCGCGACAA
GGCGCGGGGCCTCTTCACCGACCCGGCCAAGGTCCACCGCATTCTCCATGACGGGGCCTATTACAAGGTGGACGGCCGGG
CGCTGGCCGAACCCTCTCCCCAGCGCACGCCCGTGCTCTATCAGGCGGGCACCTCCGCGCGGGGACGGGTGTTCTGCGGT
CGCCATGCGGAGGCGACCTTCTTGAATGGCCAGACGCCGTCCATTGCCGGCCAGGCGGTGCGGGGCGTGCGCGAGGCTGC
GGTGGCCGCCGGGCGCGGCCCGCGCGACGTGCTCACCTTCCTCGGTGGCACGGTCATCGTGGCTAAGACCTCCGCCGAGG
CCCATGAGCTGAAGGAAGAGTACCGACGCCACATCAACGCCGCCGGGCAGTTGGCGCTGGTTTCCGGCTGGACCGGCGTT
GACCTCTCCCAGCTTTCGCTGGACGATGCGCTGAGCTTTTCCAAGAGCAACGCCATCCAGTCCACGCTGGAAAACCTCAC
CCTCAAGGCCAAGGCGCCAACCCGCGTGCGCGACCTGCTGGACTTCACCCCCGCCGGCGCCCGCGCGCCCATCTTCGTCG
GCTCTCCAGTGGAGGTCGCGGACCAGATGCTGGCCTGGGCGGATGAAGCGGACGTGGACGGCTTCAATCTCGTGCGCACC
GTCATGCCGGAATCGCTGGAAGCCATCGTCGATCTGCTGATTCCGGAATTGCAGAACCGGGGGGCTTTCAAGACCGCCTA
TCGCGAGGGGACGCTGCGGGAAAAGCTCGGCGGCACGGCCCGCCTGCCCGACAGTCATCCGGGCGCCGCCTACCGGCACC
GGAGCGCGCCCTGA

Upstream 100 bases:

>100_bases
CTTCACCCTCAAGCGGTGGAGGGCGTCTCACGTTTTGGGGCATCATCGCCCATTGTCTGCCGCTCCCCACTCCTGCCCTG
CTCCAAGGACTGCCCATCCC

Downstream 100 bases:

>100_bases
CGGGGCGGTCGGGACGGGGCTGGTCTGATTCGAGCGAACACGCGGGCGGACAATCCCGGTTCTACATCGTTTGAATGAAA
TCCATCGAGCGACCGCCGTC

Product: hypothetical protein

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSPRRELRLNTFQMAAPSHNWAGLWSHPRDNQTQYNTLAYWTEMAKTAERGLLDGIFIADVFGLYDVYGGSGDAAIAAGA
QAPNADPTLAISAMALVTQHIGFGVTANLTYEHPFQFARRFTTLDHLTGGRLGWNIVTGYLDSGARGMGESLNRDHDERY
EAAEDFMAAIYKLWEGSWEDDAALRDKARGLFTDPAKVHRILHDGAYYKVDGRALAEPSPQRTPVLYQAGTSARGRVFCG
RHAEATFLNGQTPSIAGQAVRGVREAAVAAGRGPRDVLTFLGGTVIVAKTSAEAHELKEEYRRHINAAGQLALVSGWTGV
DLSQLSLDDALSFSKSNAIQSTLENLTLKAKAPTRVRDLLDFTPAGARAPIFVGSPVEVADQMLAWADEADVDGFNLVRT
VMPESLEAIVDLLIPELQNRGAFKTAYREGTLREKLGGTARLPDSHPGAAYRHRSAP

Sequences:

>Translated_457_residues
MSPRRELRLNTFQMAAPSHNWAGLWSHPRDNQTQYNTLAYWTEMAKTAERGLLDGIFIADVFGLYDVYGGSGDAAIAAGA
QAPNADPTLAISAMALVTQHIGFGVTANLTYEHPFQFARRFTTLDHLTGGRLGWNIVTGYLDSGARGMGESLNRDHDERY
EAAEDFMAAIYKLWEGSWEDDAALRDKARGLFTDPAKVHRILHDGAYYKVDGRALAEPSPQRTPVLYQAGTSARGRVFCG
RHAEATFLNGQTPSIAGQAVRGVREAAVAAGRGPRDVLTFLGGTVIVAKTSAEAHELKEEYRRHINAAGQLALVSGWTGV
DLSQLSLDDALSFSKSNAIQSTLENLTLKAKAPTRVRDLLDFTPAGARAPIFVGSPVEVADQMLAWADEADVDGFNLVRT
VMPESLEAIVDLLIPELQNRGAFKTAYREGTLREKLGGTARLPDSHPGAAYRHRSAP
>Mature_456_residues
SPRRELRLNTFQMAAPSHNWAGLWSHPRDNQTQYNTLAYWTEMAKTAERGLLDGIFIADVFGLYDVYGGSGDAAIAAGAQ
APNADPTLAISAMALVTQHIGFGVTANLTYEHPFQFARRFTTLDHLTGGRLGWNIVTGYLDSGARGMGESLNRDHDERYE
AAEDFMAAIYKLWEGSWEDDAALRDKARGLFTDPAKVHRILHDGAYYKVDGRALAEPSPQRTPVLYQAGTSARGRVFCGR
HAEATFLNGQTPSIAGQAVRGVREAAVAAGRGPRDVLTFLGGTVIVAKTSAEAHELKEEYRRHINAAGQLALVSGWTGVD
LSQLSLDDALSFSKSNAIQSTLENLTLKAKAPTRVRDLLDFTPAGARAPIFVGSPVEVADQMLAWADEADVDGFNLVRTV
MPESLEAIVDLLIPELQNRGAFKTAYREGTLREKLGGTARLPDSHPGAAYRHRSAP

Specific function: Involved In Desulfonation Of Aliphatic Sulfonates. Catalyzes The Conversion Of Pentanesulfonic Acid To Sulfite And Pentaldehyde And Is Able To Desulfonate A Wide Range Of Sulfonated Substrates Including C-2 To C-10 Unsubstituted Linear Alkanesulfonates, S

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=222, Percent_Identity=27.9279279279279, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR016215 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 49679; Mature: 49548

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRRELRLNTFQMAAPSHNWAGLWSHPRDNQTQYNTLAYWTEMAKTAERGLLDGIFIAD
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFGLYDVYGGSGDAAIAAGAQAPNADPTLAISAMALVTQHIGFGVTANLTYEHPFQFARR
HHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
FTTLDHLTGGRLGWNIVTGYLDSGARGMGESLNRDHDERYEAAEDFMAAIYKLWEGSWED
HHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DAALRDKARGLFTDPAKVHRILHDGAYYKVDGRALAEPSPQRTPVLYQAGTSARGRVFCG
CHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCEECCCCCCCCCEEEECCCCCCCCEEEC
RHAEATFLNGQTPSIAGQAVRGVREAAVAAGRGPRDVLTFLGGTVIVAKTSAEAHELKEE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCHHHHHHHHH
YRRHINAAGQLALVSGWTGVDLSQLSLDDALSFSKSNAIQSTLENLTLKAKAPTRVRDLL
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHH
DFTPAGARAPIFVGSPVEVADQMLAWADEADVDGFNLVRTVMPESLEAIVDLLIPELQNR
HCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAFKTAYREGTLREKLGGTARLPDSHPGAAYRHRSAP
CCHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPRRELRLNTFQMAAPSHNWAGLWSHPRDNQTQYNTLAYWTEMAKTAERGLLDGIFIAD
CCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFGLYDVYGGSGDAAIAAGAQAPNADPTLAISAMALVTQHIGFGVTANLTYEHPFQFARR
HHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
FTTLDHLTGGRLGWNIVTGYLDSGARGMGESLNRDHDERYEAAEDFMAAIYKLWEGSWED
HHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DAALRDKARGLFTDPAKVHRILHDGAYYKVDGRALAEPSPQRTPVLYQAGTSARGRVFCG
CHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCEECCCCCCCCCEEEECCCCCCCCEEEC
RHAEATFLNGQTPSIAGQAVRGVREAAVAAGRGPRDVLTFLGGTVIVAKTSAEAHELKEE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCHHHHHHHHH
YRRHINAAGQLALVSGWTGVDLSQLSLDDALSFSKSNAIQSTLENLTLKAKAPTRVRDLL
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHH
DFTPAGARAPIFVGSPVEVADQMLAWADEADVDGFNLVRTVMPESLEAIVDLLIPELQNR
HCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAFKTAYREGTLREKLGGTARLPDSHPGAAYRHRSAP
CCHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]