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The map label for this gene is nfrA1 [H]

Identifier: 158424800

GI number: 158424800

Start: 3631505

End: 3632359

Strand: Reverse

Name: nfrA1 [H]

Synonym: AZC_3176

Alternate gene names: 158424800

Gene position: 3632359-3631505 (Counterclockwise)

Preceding gene: 158424804

Following gene: 158424797

Centisome position: 67.64

GC content: 69.12

Gene sequence:

>855_bases
TTGATGAGCGATACTCTGATTGCCGAAAAGCCCGTCGTGAGCGCGGCTGAAGCCGCGCTGGAGCGGCGTTACCGCGAGCC
GCTGGCCGGCGCCGACCACCCCTGGAACGCTGTCATCCAGAGCCTCGTGGACCACCGCACCGTGCGGGCCTTCCTGCCGA
AGGCGGTGAGCGAGGAGACGCTGCAGGTGCTGGTGGCCGCCGCCCAATCGGCGCCCACCTCGTCCAATGTGCAGGCCTGG
AGCGTGGTGGCGGTGCGCGATCCTGCCCGCAAGGCGCGCCTCGCCCACCTCGCCGGCGACCAGAAGCACATTCTGGAGGC
GCCGCTGCTGCTCGTGTGGATCGCCGATCTCTCCCGCGCCGCCGGCATCGGGGAACGCGCGGGCCTGACGCTGGAAGGGC
TCGATTTCACGGAGAGCTTCCTCATCGCCGGCTTCGATGCGGCCCTCGCCGCCCAGAATGCGCTGGTGGCGGCCGAATCC
CTGGGGCTCGGTACCTGCTATATCGGCGCCTTGCGCAACCACCCCATCGAGGTCGCGGAAACGCTGGGCCTGCCGCGGAA
GACCTTCGCCGTATTCGGCCTCGTGGTGGGCCATCCCGATCCCGCGCGCCCGGCGGACGTGAAACCGCGCCTGCCGCAGG
CGGCGGTGCTGCATCACGAGACCTACGATCTTGCCGGGCAGGCGGAGGCGGTGGCGCGCCATGATGCCCATAGCCTGGCC
TTCCGCGCCGAGCAGAAGCTCGACGCCACCCCCTGGAGCCAGCAACTGATCGGCCGCCTGCATACGGTGGCAGCCCTCAA
GGGGCGTCATCTCCTCAAGCAGGCGTTGGAGAAACTCGGCTTTGCGCTGAAATGA

Upstream 100 bases:

>100_bases
GTGGCGCCGCCGGCGCAACCGGCGACAGCTTGGCAATCGCCGCCCCGTCTCCCACCTTCTGTCGGATCGAACCCCTTCGC
GCGGCCCCTGCCGGAGACCC

Downstream 100 bases:

>100_bases
AAGACGGGCGCCCCGCTTCAGTGCGGGGCGCCCGTGGTTTCCGGCCCAGCCTCAGCTGTAGAGCGGCGTCGCCGTGATGG
TGCCATTGAGCGCGTAATTG

Product: nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MMSDTLIAEKPVVSAAEAALERRYREPLAGADHPWNAVIQSLVDHRTVRAFLPKAVSEETLQVLVAAAQSAPTSSNVQAW
SVVAVRDPARKARLAHLAGDQKHILEAPLLLVWIADLSRAAGIGERAGLTLEGLDFTESFLIAGFDAALAAQNALVAAES
LGLGTCYIGALRNHPIEVAETLGLPRKTFAVFGLVVGHPDPARPADVKPRLPQAAVLHHETYDLAGQAEAVARHDAHSLA
FRAEQKLDATPWSQQLIGRLHTVAALKGRHLLKQALEKLGFALK

Sequences:

>Translated_284_residues
MMSDTLIAEKPVVSAAEAALERRYREPLAGADHPWNAVIQSLVDHRTVRAFLPKAVSEETLQVLVAAAQSAPTSSNVQAW
SVVAVRDPARKARLAHLAGDQKHILEAPLLLVWIADLSRAAGIGERAGLTLEGLDFTESFLIAGFDAALAAQNALVAAES
LGLGTCYIGALRNHPIEVAETLGLPRKTFAVFGLVVGHPDPARPADVKPRLPQAAVLHHETYDLAGQAEAVARHDAHSLA
FRAEQKLDATPWSQQLIGRLHTVAALKGRHLLKQALEKLGFALK
>Mature_284_residues
MMSDTLIAEKPVVSAAEAALERRYREPLAGADHPWNAVIQSLVDHRTVRAFLPKAVSEETLQVLVAAAQSAPTSSNVQAW
SVVAVRDPARKARLAHLAGDQKHILEAPLLLVWIADLSRAAGIGERAGLTLEGLDFTESFLIAGFDAALAAQNALVAAES
LGLGTCYIGALRNHPIEVAETLGLPRKTFAVFGLVVGHPDPARPADVKPRLPQAAVLHHETYDLAGQAEAVARHDAHSLA
FRAEQKLDATPWSQQLIGRLHTVAALKGRHLLKQALEKLGFALK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=198, Percent_Identity=36.8686868686869, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 30428; Mature: 30428

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSDTLIAEKPVVSAAEAALERRYREPLAGADHPWNAVIQSLVDHRTVRAFLPKAVSEET
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVLVAAAQSAPTSSNVQAWSVVAVRDPARKARLAHLAGDQKHILEAPLLLVWIADLSRA
HHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGIGERAGLTLEGLDFTESFLIAGFDAALAAQNALVAAESLGLGTCYIGALRNHPIEVAE
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
TLGLPRKTFAVFGLVVGHPDPARPADVKPRLPQAAVLHHETYDLAGQAEAVARHDAHSLA
HCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FRAEQKLDATPWSQQLIGRLHTVAALKGRHLLKQALEKLGFALK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMSDTLIAEKPVVSAAEAALERRYREPLAGADHPWNAVIQSLVDHRTVRAFLPKAVSEET
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVLVAAAQSAPTSSNVQAWSVVAVRDPARKARLAHLAGDQKHILEAPLLLVWIADLSRA
HHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGIGERAGLTLEGLDFTESFLIAGFDAALAAQNALVAAESLGLGTCYIGALRNHPIEVAE
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
TLGLPRKTFAVFGLVVGHPDPARPADVKPRLPQAAVLHHETYDLAGQAEAVARHDAHSLA
HCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FRAEQKLDATPWSQQLIGRLHTVAALKGRHLLKQALEKLGFALK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]