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The map label for this gene is nifR3 [H]

Identifier: 158424712

GI number: 158424712

Start: 3534253

End: 3535350

Strand: Reverse

Name: nifR3 [H]

Synonym: AZC_3088

Alternate gene names: 158424712

Gene position: 3535350-3534253 (Counterclockwise)

Preceding gene: 158424720

Following gene: 158424711

Centisome position: 65.84

GC content: 70.4

Gene sequence:

>1098_bases
ATGGCCTTGCGCAACGGCTTCGAATGTCTAGAATGTAGGCAGCTGATGGATGGCCTAATTTCTGTGCAAACCGCTCCCTT
TCCTGCCTTTTCGCCGCTGACGATCGGCCCGCACCGCATTGCGGCGCCGGTCCTTCTGGCGCCGATGGCGGGCATCACCG
ACGCCCCGGTGCGGCGCATGGCGCTGCGCTACGGGGCCGGGCTGACGGTGTCCGAGATGGTGGCGAGCGAGGCCCTGCTC
ACCGGCCATGCGGAAATGACGCTGCGGGCCGAAGGCGCGGGCGTGGACATCCATGCCGTGCAACTGGCCGGCAACGAGCC
GCACTGGATGGCCGAGGCGGCGCGCCTGGCGGAAGCCTCCGGCGCCGCCATCATCGACATCAACATGGGCTGTCCCGCCA
AGCGCGTGACCACAGGTGCGGCGGGCGCGGCCCTCCTGCGTGACCTGCCGCTGGCGACCCGGATTCTCGAAGCGGTGCGC
GCGGCCATCTCGGTTCCTGTCACGCTGAAGACCCGGCTCGGCTGGGACGGCGCTGAAATGACCGCGCCAGAGATCGCCCG
CCGTGCAGAGGCGATCGGCATCGCAATGGTCACCATCCACGGGCGCACCCGCTGCCAGTTCTACACCGGCACCGCCGACT
GGGCGGCCATCCGCGCGGTGAAGGACGCCGTCTCCATTCCGGTGATCGCTAATGGCGACCTCACCCAGACGCACGATGCC
GCCGCCATGCTGGCCGCTTCCGGCGCGGACGGCGTGATGATCGGGCGCGGGGCGCAGGGGCGGCCCTGGTTCCCGGGCGC
CGTCGCCCAGCACTGGAGGACGGGCCGCGCGCCGGCAGACCCCGACCTTGCCGAGCAGCGCGACGTGCTGCTCGAACTCT
ATGACGGCTGGCTGGCGCTCTATGGCGTCGAGCTCGGCCTGCGGCAGGCCCGCAAGCATGTGGGCTGGGCGCTGGAGGCG
GCTGCGGCCTCGGCCGGCGCTGCGCCTCAAATCGCCAAGGATGCGCGTGGCCGCATCCTCACTCTGGAGGCCCCCGACGC
TGTGATCGCGGGCATCCGCACCTTTTACGACCAACTCGCCTGGAGGGCGGCCGCATGA

Upstream 100 bases:

>100_bases
CCGCGCCGCAGGCGCAAGGGATAATGCGGAGGCAGCGAGCCGCCGCCCCATCGGGCAGCACGGGCAAGGCGCAGGCAAAA
AGCCTGCAAACTTCATCGCT

Downstream 100 bases:

>100_bases
AGGCGGAACTGAAGCCTCGCACCTCGGACGGCGAGGCCCTGACCCGCGCCATCCTGGATGCGGTGCCGCATCCGGTGGTG
ACGCTGGCGGAAGACGGCCG

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MALRNGFECLECRQLMDGLISVQTAPFPAFSPLTIGPHRIAAPVLLAPMAGITDAPVRRMALRYGAGLTVSEMVASEALL
TGHAEMTLRAEGAGVDIHAVQLAGNEPHWMAEAARLAEASGAAIIDINMGCPAKRVTTGAAGAALLRDLPLATRILEAVR
AAISVPVTLKTRLGWDGAEMTAPEIARRAEAIGIAMVTIHGRTRCQFYTGTADWAAIRAVKDAVSIPVIANGDLTQTHDA
AAMLAASGADGVMIGRGAQGRPWFPGAVAQHWRTGRAPADPDLAEQRDVLLELYDGWLALYGVELGLRQARKHVGWALEA
AAASAGAAPQIAKDARGRILTLEAPDAVIAGIRTFYDQLAWRAAA

Sequences:

>Translated_365_residues
MALRNGFECLECRQLMDGLISVQTAPFPAFSPLTIGPHRIAAPVLLAPMAGITDAPVRRMALRYGAGLTVSEMVASEALL
TGHAEMTLRAEGAGVDIHAVQLAGNEPHWMAEAARLAEASGAAIIDINMGCPAKRVTTGAAGAALLRDLPLATRILEAVR
AAISVPVTLKTRLGWDGAEMTAPEIARRAEAIGIAMVTIHGRTRCQFYTGTADWAAIRAVKDAVSIPVIANGDLTQTHDA
AAMLAASGADGVMIGRGAQGRPWFPGAVAQHWRTGRAPADPDLAEQRDVLLELYDGWLALYGVELGLRQARKHVGWALEA
AAASAGAAPQIAKDARGRILTLEAPDAVIAGIRTFYDQLAWRAAA
>Mature_364_residues
ALRNGFECLECRQLMDGLISVQTAPFPAFSPLTIGPHRIAAPVLLAPMAGITDAPVRRMALRYGAGLTVSEMVASEALLT
GHAEMTLRAEGAGVDIHAVQLAGNEPHWMAEAARLAEASGAAIIDINMGCPAKRVTTGAAGAALLRDLPLATRILEAVRA
AISVPVTLKTRLGWDGAEMTAPEIARRAEAIGIAMVTIHGRTRCQFYTGTADWAAIRAVKDAVSIPVIANGDLTQTHDAA
AMLAASGADGVMIGRGAQGRPWFPGAVAQHWRTGRAPADPDLAEQRDVLLELYDGWLALYGVELGLRQARKHVGWALEAA
AASAGAAPQIAKDARGRILTLEAPDAVIAGIRTFYDQLAWRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=281, Percent_Identity=31.3167259786477, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI239788462, Length=281, Percent_Identity=31.3167259786477, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI40807366, Length=256, Percent_Identity=31.25, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=30.1801801801802, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=239, Percent_Identity=29.7071129707113, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789660, Length=299, Percent_Identity=46.1538461538462, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=36.5217391304348, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI145693211, Length=260, Percent_Identity=29.2307692307692, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=293, Percent_Identity=29.3515358361775, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=166, Percent_Identity=36.7469879518072, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=30.5439330543933, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=238, Percent_Identity=30.672268907563, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=239, Percent_Identity=29.2887029288703, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=298, Percent_Identity=27.1812080536913, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=238, Percent_Identity=28.1512605042017, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24585320, Length=229, Percent_Identity=36.6812227074236, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=32.618025751073, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=32.618025751073, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19921524, Length=223, Percent_Identity=29.1479820627803, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI45549423, Length=236, Percent_Identity=33.0508474576271, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38349; Mature: 38217

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRNGFECLECRQLMDGLISVQTAPFPAFSPLTIGPHRIAAPVLLAPMAGITDAPVRRM
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ALRYGAGLTVSEMVASEALLTGHAEMTLRAEGAGVDIHAVQLAGNEPHWMAEAARLAEAS
HHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCC
GAAIIDINMGCPAKRVTTGAAGAALLRDLPLATRILEAVRAAISVPVTLKTRLGWDGAEM
CCEEEEEECCCCHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
TAPEIARRAEAIGIAMVTIHGRTRCQFYTGTADWAAIRAVKDAVSIPVIANGDLTQTHDA
CHHHHHHHHHHHEEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCHHH
AAMLAASGADGVMIGRGAQGRPWFPGAVAQHWRTGRAPADPDLAEQRDVLLELYDGWLAL
HHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGVELGLRQARKHVGWALEAAAASAGAAPQIAKDARGRILTLEAPDAVIAGIRTFYDQLA
HHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHH
WRAAA
HHCCC
>Mature Secondary Structure 
ALRNGFECLECRQLMDGLISVQTAPFPAFSPLTIGPHRIAAPVLLAPMAGITDAPVRRM
CCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ALRYGAGLTVSEMVASEALLTGHAEMTLRAEGAGVDIHAVQLAGNEPHWMAEAARLAEAS
HHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCC
GAAIIDINMGCPAKRVTTGAAGAALLRDLPLATRILEAVRAAISVPVTLKTRLGWDGAEM
CCEEEEEECCCCHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
TAPEIARRAEAIGIAMVTIHGRTRCQFYTGTADWAAIRAVKDAVSIPVIANGDLTQTHDA
CHHHHHHHHHHHEEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCHHH
AAMLAASGADGVMIGRGAQGRPWFPGAVAQHWRTGRAPADPDLAEQRDVLLELYDGWLAL
HHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGVELGLRQARKHVGWALEAAAASAGAAPQIAKDARGRILTLEAPDAVIAGIRTFYDQLA
HHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHH
WRAAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]