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The map label for this gene is pyrG [H]

Identifier: 158424691

GI number: 158424691

Start: 3505322

End: 3506950

Strand: Reverse

Name: pyrG [H]

Synonym: AZC_3067

Alternate gene names: 158424691

Gene position: 3506950-3505322 (Counterclockwise)

Preceding gene: 158424692

Following gene: 158424690

Centisome position: 65.31

GC content: 65.93

Gene sequence:

>1629_bases
ATGGCGCGCTACATCTTCATCACCGGCGGCGTCGTGTCCTCCCTTGGCAAGGGCCTCGCCTCCGCAGCACTCGGCGCGCT
TCTTCAGGCGCGCGGGTACACCGTCCGGCTCCGCAAGCTGGACCCCTATCTGAACGTCGATCCGGGCACCATGAGCCCGT
ATCAGCATGGCGAGGTCTTCGTGACCGACGACGGGGCCGAGACCGATCTCGACCTCGGCCATTACGAGCGCTTCACCGGG
CATCCTGCCACCAAGCAGGACAACATCACCACCGGGCGCATCTATCAGGACATCCTCACCAAGGAGCGGCGCGGCGACTA
TCTGGGCGCCACCATCCAGGTGATTCCCCACGTCACCAACGCCATCAAGGACTTCGTGCTGGAAGGCAACGAGGGCTTCG
ACTTCGTGCTCATCGAGGTCGGCGGCACGGTGGGTGACATCGAGGGCCTGCCCTTCTTCGAGGCGACCCGTCAGCTCAAG
AACGAGCTGCCGCGCAACCACGCCATCTTCATCCACCTGACGCTGATGCCCTTCATCCCCTCCGCCGGGGAACTGAAGAC
CAAGCCGACGCAGCACTCCGTCAAGGAACTGCGCTCCATCGGCATCCAGCCCGACATCCTGCTCTGCCGCTGCGATCGCG
AGATCCCGCGCGAGGAGCGGCGCAAGCTCTCGCTCTTCTGCAACGTGCGCGAGAGCGCCGTGATCGAGGCGCGCGACGTG
GACAACATCTACGCCGTGCCGTCCTCCTACCATGCGGAAGGTCTCGACACGGAAGTGCTGGCCGCCTTCGGCATCGAGCC
GGCCCCGGCGCCGAACCTCCAGCGCTGGACCAATATCGAGCAGCGCGTGCGCAACCCCGAGGGCGAAGTGACCATCGCCA
TCGTGGGCAAGTACACGGGCCTCAAGGACGCCTACAAGTCGCTCATCGAGGCGCTGAACCACGGCGGCATCGCCAACAAG
GTGAAGGTGAACCTCGACTGGATCGAGAGCGAGACCTTCGAGACCGAGGACCCGGCGCCCTTCCTGGAGCATGTCCACGG
CATTCTCGTGCCGGGCGGCTTCGGCCAGCGCGGCGCCGAGGGCAAGATCCGCGCGGCCCAGTTCGCCCGCGAGCGCAAGG
TGCCCTATTTCGGCATCTGCTTCGGCATGCAGATGGCCGTGATCGAGGCGGCCCGGAACCTGGCCGGCGTCAAGAACGCC
AACTCCACCGAGTTCGGCGAGACGTCCGAGCCGGTGGTGGGCCTGCTCACCGAATGGCTGAAGGGCAATGAACTGGAGCG
CCGCTCGGCAGCGGGCGACCTCGGCGGCACCATGCGCCTCGGCGCCTATCTGGCGCAGCTCAAGGAGCCGTCCCGCGTCG
CCGGCATCTATGGCGCGCAGGAGATTTCCGAGCGTCACCGTCACCGTTACGAGGTGAATACCGCCTACAAGGATCGCCTC
GAGGCCTGCGGTCTCATGTTCTCCGGCCTGTCGCCCGATGGCCTGCTGCCGGAGATCGTGGAATATGCGGACCATCCCTG
GTTCATCGGCGTGCAGTTCCATCCCGAGCTGAAGTCGCGCCCGTTCCAGCCGCATCCGCTCTTCGCCTCCTTCATCGGGG
CGGCCATGGAGCAGAGCCGGCTGGTGTGA

Upstream 100 bases:

>100_bases
CGCGGCAGGCCGCGGCGTGTAGGTTCTCCACAGGCGACGCGGTGCCCCAAAGGGCGTCGCGCTCGCCGAATCGCGTTTGC
GCGGAGTAGAGTTGAAGCCC

Downstream 100 bases:

>100_bases
GTGAGCCTGCCGCTCCGGCGTGAAGCCGGGCGGCGTGCGATCAGGTAAAGGAGTTGCCCATGCGTCGCGGCCTCGATCAC
GTCGTCCATCTGGTGCGGGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
HPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTNAIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLK
NELPRNHAIFIHLTLMPFIPSAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPREERRKLSLFCNVRESAVIEARDV
DNIYAVPSSYHAEGLDTEVLAAFGIEPAPAPNLQRWTNIEQRVRNPEGEVTIAIVGKYTGLKDAYKSLIEALNHGGIANK
VKVNLDWIESETFETEDPAPFLEHVHGILVPGGFGQRGAEGKIRAAQFARERKVPYFGICFGMQMAVIEAARNLAGVKNA
NSTEFGETSEPVVGLLTEWLKGNELERRSAAGDLGGTMRLGAYLAQLKEPSRVAGIYGAQEISERHRHRYEVNTAYKDRL
EACGLMFSGLSPDGLLPEIVEYADHPWFIGVQFHPELKSRPFQPHPLFASFIGAAMEQSRLV

Sequences:

>Translated_542_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
HPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTNAIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLK
NELPRNHAIFIHLTLMPFIPSAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPREERRKLSLFCNVRESAVIEARDV
DNIYAVPSSYHAEGLDTEVLAAFGIEPAPAPNLQRWTNIEQRVRNPEGEVTIAIVGKYTGLKDAYKSLIEALNHGGIANK
VKVNLDWIESETFETEDPAPFLEHVHGILVPGGFGQRGAEGKIRAAQFARERKVPYFGICFGMQMAVIEAARNLAGVKNA
NSTEFGETSEPVVGLLTEWLKGNELERRSAAGDLGGTMRLGAYLAQLKEPSRVAGIYGAQEISERHRHRYEVNTAYKDRL
EACGLMFSGLSPDGLLPEIVEYADHPWFIGVQFHPELKSRPFQPHPLFASFIGAAMEQSRLV
>Mature_541_residues
ARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGH
PATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTNAIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLKN
ELPRNHAIFIHLTLMPFIPSAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPREERRKLSLFCNVRESAVIEARDVD
NIYAVPSSYHAEGLDTEVLAAFGIEPAPAPNLQRWTNIEQRVRNPEGEVTIAIVGKYTGLKDAYKSLIEALNHGGIANKV
KVNLDWIESETFETEDPAPFLEHVHGILVPGGFGQRGAEGKIRAAQFARERKVPYFGICFGMQMAVIEAARNLAGVKNAN
STEFGETSEPVVGLLTEWLKGNELERRSAAGDLGGTMRLGAYLAQLKEPSRVAGIYGAQEISERHRHRYEVNTAYKDRLE
ACGLMFSGLSPDGLLPEIVEYADHPWFIGVQFHPELKSRPFQPHPLFASFIGAAMEQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=558, Percent_Identity=44.8028673835125, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=555, Percent_Identity=44.1441441441441, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI28559083, Length=555, Percent_Identity=44.1441441441441, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI221316689, Length=555, Percent_Identity=44.1441441441441, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=58.955223880597, Blast_Score=623, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI25148299, Length=612, Percent_Identity=36.7647058823529, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=40.2460456942003, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319432, Length=568, Percent_Identity=39.0845070422535, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=43.649373881932, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.7722772277228, Blast_Score=390, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59980; Mature: 59849

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00442 GATASE_TYPE_I ; PS00214 FABP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGHPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLKNELPRNHAIFIHLTLMPFIP
HHHHHHCCCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPREERRKLSLFCNVRESAVIEARDV
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHEEHEEECCHHHEEEECCC
DNIYAVPSSYHAEGLDTEVLAAFGIEPAPAPNLQRWTNIEQRVRNPEGEVTIAIVGKYTG
CCEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHCHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALNHGGIANKVKVNLDWIESETFETEDPAPFLEHVHGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHCCEEEECCCCCCCCCC
GKIRAAQFARERKVPYFGICFGMQMAVIEAARNLAGVKNANSTEFGETSEPVVGLLTEWL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KGNELERRSAAGDLGGTMRLGAYLAQLKEPSRVAGIYGAQEISERHRHRYEVNTAYKDRL
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHEEECCHHHHHHH
EACGLMFSGLSPDGLLPEIVEYADHPWFIGVQFHPELKSRPFQPHPLFASFIGAAMEQSR
HHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGHPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLKNELPRNHAIFIHLTLMPFIP
HHHHHHCCCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPREERRKLSLFCNVRESAVIEARDV
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHEEHEEECCHHHEEEECCC
DNIYAVPSSYHAEGLDTEVLAAFGIEPAPAPNLQRWTNIEQRVRNPEGEVTIAIVGKYTG
CCEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHCHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALNHGGIANKVKVNLDWIESETFETEDPAPFLEHVHGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHCCEEEECCCCCCCCCC
GKIRAAQFARERKVPYFGICFGMQMAVIEAARNLAGVKNANSTEFGETSEPVVGLLTEWL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KGNELERRSAAGDLGGTMRLGAYLAQLKEPSRVAGIYGAQEISERHRHRYEVNTAYKDRL
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHEEECCHHHHHHH
EACGLMFSGLSPDGLLPEIVEYADHPWFIGVQFHPELKSRPFQPHPLFASFIGAAMEQSR
HHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]