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The map label for this gene is citH [H]

Identifier: 158424602

GI number: 158424602

Start: 3403147

End: 3404499

Strand: Direct

Name: citH [H]

Synonym: AZC_2978

Alternate gene names: 158424602

Gene position: 3403147-3404499 (Clockwise)

Preceding gene: 158424600

Following gene: 158424603

Centisome position: 63.38

GC content: 65.19

Gene sequence:

>1353_bases
TTGTGCGCCATAGTGCCGCCCATTTTTCGACCCGAGAGGCCCCCAATGCCCGCCCCGCAGCCTCAGGCAGCGCCCTCCTC
CAAGGCCATGACAGTGCTCCGCGTCACCAGCGGCAACTTCCTGGAGATGTTCGATTTCTTCCTGTTCGGATTCTATGCGA
CGCACATCGCGCACGCCTTCTTTCCGGTCAGTGATGCCTATGCCTCGCTCATGCTGACCTTCGGCACCTTCGGCGCCGGC
TTTCTCATGCGGCCCTTAGGGGCGATCATTCTCGGCGCTTATGTGGACCGCCTCGGCCGCCGGCAGGGTCTGATCCTCAC
GCTCGGCATCATGTCGCTGGGCACCGTCCTCATCGCCTTCGTGCCCGGCTATGCCACCATCGGCATGGCCGCGCCGCTTC
TGGTGCTGCTCGGCCGGCTGCTTCAGGGCTTTTCCGCGGGCGTGGAACTGGGCGGCGTCTCCGTCTATCTCGCGGAGATC
GCGACGCCCGGGAACAAGGGCTTCTATGTCAGCTGGCAGTCGGGCAGCCAGCAGGTGGCGATCATCGTGGCTGCCCTCCT
CGGCTATGCCCTCAATGCCTGGATGGCGCCGCAGGCCGTCTCCGACTGGGCGTGGCGCATTCCCTTCTTCATCGGCTGTC
TCATCGTGCCCCTGCTGTTCCTCCTGCGTCGCTCGCTCGAGGAGACGGAGGAGTTCCTCGCCCGCAAGCATCGCCCCGAG
CTTGGGGAGATGTTCCGCACCATCGCCGGCAACTGGAAGATCGTTCTGGCGGGCATGATGCTGGTGGTGATGACCACCGT
GTCCTTCTACATGATCACCGTCTACACTCCGACCTTCGGGCGGGCAGTGCTCAAGCTCTCCCAGACAGACGCGCTGCTGG
TGACGCTGTGCGTCGGCATTTCCAACTTCTTCTGGCTGCCCGTCATGGGTGCTCTGTCGGATCGCATCGGCCGCAAGCCG
ATCCTCATCATCTTCACCGCCCTGACGCTGCTCACCGCCTATCCCGCGCTCGCGTGGCTGGTGGCCAACGTCACCTTCGG
CAAGATGCTGGCGGTCGAACTCTGGCTGTCCTTCCTCTACGCCAGCTACAACGGCGCGATGGTGGTGGCGCTCACCGAGG
TGATGCCGGCGCATGTGCGCACCACAGGCTTCTCGCTCGCCTACAGCCTCGCCACGGCGCTGTTCGGCGGCTTCACGCCG
GCGGTGTCCACCTGGCTTATCGAGCAGACGGGCGACAAGGCCGCGCCGGGCTACTGGCTGATCTTCGCCGGCGCCTGCGG
CCTCATCGCCACCTTCATCCTCTACGGGCGCCAGGGCGCCATGGTGGCAAAGCCCGCCGTGCGGACGAACTGA

Upstream 100 bases:

>100_bases
TTTGCAGACCACCGTCAACATCACCCAACCTTCCGCAACGGAAAACTAACCCTTTGATATGAATTGCTAATTCCCTAGCG
GCATGTAGGCATCTGACGCT

Downstream 100 bases:

>100_bases
TCCATCACCGGATCTCTCTTGCGATGCCTCCGGCCTCCCATGGCCGGAGGCATTTTTCTTGGAATCGAGCCCGCGCCCTG
CCCCGGCCCAGCGCCCTTCC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MCAIVPPIFRPERPPMPAPQPQAAPSSKAMTVLRVTSGNFLEMFDFFLFGFYATHIAHAFFPVSDAYASLMLTFGTFGAG
FLMRPLGAIILGAYVDRLGRRQGLILTLGIMSLGTVLIAFVPGYATIGMAAPLLVLLGRLLQGFSAGVELGGVSVYLAEI
ATPGNKGFYVSWQSGSQQVAIIVAALLGYALNAWMAPQAVSDWAWRIPFFIGCLIVPLLFLLRRSLEETEEFLARKHRPE
LGEMFRTIAGNWKIVLAGMMLVVMTTVSFYMITVYTPTFGRAVLKLSQTDALLVTLCVGISNFFWLPVMGALSDRIGRKP
ILIIFTALTLLTAYPALAWLVANVTFGKMLAVELWLSFLYASYNGAMVVALTEVMPAHVRTTGFSLAYSLATALFGGFTP
AVSTWLIEQTGDKAAPGYWLIFAGACGLIATFILYGRQGAMVAKPAVRTN

Sequences:

>Translated_450_residues
MCAIVPPIFRPERPPMPAPQPQAAPSSKAMTVLRVTSGNFLEMFDFFLFGFYATHIAHAFFPVSDAYASLMLTFGTFGAG
FLMRPLGAIILGAYVDRLGRRQGLILTLGIMSLGTVLIAFVPGYATIGMAAPLLVLLGRLLQGFSAGVELGGVSVYLAEI
ATPGNKGFYVSWQSGSQQVAIIVAALLGYALNAWMAPQAVSDWAWRIPFFIGCLIVPLLFLLRRSLEETEEFLARKHRPE
LGEMFRTIAGNWKIVLAGMMLVVMTTVSFYMITVYTPTFGRAVLKLSQTDALLVTLCVGISNFFWLPVMGALSDRIGRKP
ILIIFTALTLLTAYPALAWLVANVTFGKMLAVELWLSFLYASYNGAMVVALTEVMPAHVRTTGFSLAYSLATALFGGFTP
AVSTWLIEQTGDKAAPGYWLIFAGACGLIATFILYGRQGAMVAKPAVRTN
>Mature_450_residues
MCAIVPPIFRPERPPMPAPQPQAAPSSKAMTVLRVTSGNFLEMFDFFLFGFYATHIAHAFFPVSDAYASLMLTFGTFGAG
FLMRPLGAIILGAYVDRLGRRQGLILTLGIMSLGTVLIAFVPGYATIGMAAPLLVLLGRLLQGFSAGVELGGVSVYLAEI
ATPGNKGFYVSWQSGSQQVAIIVAALLGYALNAWMAPQAVSDWAWRIPFFIGCLIVPLLFLLRRSLEETEEFLARKHRPE
LGEMFRTIAGNWKIVLAGMMLVVMTTVSFYMITVYTPTFGRAVLKLSQTDALLVTLCVGISNFFWLPVMGALSDRIGRKP
ILIIFTALTLLTAYPALAWLVANVTFGKMLAVELWLSFLYASYNGAMVVALTEVMPAHVRTTGFSLAYSLATALFGGFTP
AVSTWLIEQTGDKAAPGYWLIFAGACGLIATFILYGRQGAMVAKPAVRTN

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=403, Percent_Identity=32.7543424317618, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788942, Length=384, Percent_Identity=35.6770833333333, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788292, Length=388, Percent_Identity=29.8969072164948, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789941, Length=397, Percent_Identity=30.2267002518892, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87082404, Length=207, Percent_Identity=28.9855072463768, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082231, Length=203, Percent_Identity=29.064039408867, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48785; Mature: 48785

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAIVPPIFRPERPPMPAPQPQAAPSSKAMTVLRVTSGNFLEMFDFFLFGFYATHIAHAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
FPVSDAYASLMLTFGTFGAGFLMRPLGAIILGAYVDRLGRRQGLILTLGIMSLGTVLIAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
VPGYATIGMAAPLLVLLGRLLQGFSAGVELGGVSVYLAEIATPGNKGFYVSWQSGSQQVA
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCEEEEECCCCCHHH
IIVAALLGYALNAWMAPQAVSDWAWRIPFFIGCLIVPLLFLLRRSLEETEEFLARKHRPE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LGEMFRTIAGNWKIVLAGMMLVVMTTVSFYMITVYTPTFGRAVLKLSQTDALLVTLCVGI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCHHHHHHHHHHH
SNFFWLPVMGALSDRIGRKPILIIFTALTLLTAYPALAWLVANVTFGKMLAVELWLSFLY
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYNGAMVVALTEVMPAHVRTTGFSLAYSLATALFGGFTPAVSTWLIEQTGDKAAPGYWL
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEE
IFAGACGLIATFILYGRQGAMVAKPAVRTN
EHHHHHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MCAIVPPIFRPERPPMPAPQPQAAPSSKAMTVLRVTSGNFLEMFDFFLFGFYATHIAHAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
FPVSDAYASLMLTFGTFGAGFLMRPLGAIILGAYVDRLGRRQGLILTLGIMSLGTVLIAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
VPGYATIGMAAPLLVLLGRLLQGFSAGVELGGVSVYLAEIATPGNKGFYVSWQSGSQQVA
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCEEEEECCCCCHHH
IIVAALLGYALNAWMAPQAVSDWAWRIPFFIGCLIVPLLFLLRRSLEETEEFLARKHRPE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LGEMFRTIAGNWKIVLAGMMLVVMTTVSFYMITVYTPTFGRAVLKLSQTDALLVTLCVGI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCHHHHHHHHHHH
SNFFWLPVMGALSDRIGRKPILIIFTALTLLTAYPALAWLVANVTFGKMLAVELWLSFLY
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYNGAMVVALTEVMPAHVRTTGFSLAYSLATALFGGFTPAVSTWLIEQTGDKAAPGYWL
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEE
IFAGACGLIATFILYGRQGAMVAKPAVRTN
EHHHHHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]