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The map label for this gene is cobS

Identifier: 158424520

GI number: 158424520

Start: 3307747

End: 3308541

Strand: Direct

Name: cobS

Synonym: AZC_2896

Alternate gene names: 158424520

Gene position: 3307747-3308541 (Clockwise)

Preceding gene: 158424514

Following gene: 158424521

Centisome position: 61.6

GC content: 72.83

Gene sequence:

>795_bases
ATGCGGCACAGCAGGATCTTGGCGGATGTCGGCGCGGCCCTGCGCTTCTACTCGCGCATCCCCGTCCCCGCCGGCGCCGC
CGAGGACGCCTTCGCGCCCCCGGACCTGAAGCGCATCGCCTATGCGGTGCCGCTGGCCGGTGCCGCCATCGGCCTTGCCG
GCGCAGCGATTCTCATCGGCGCGCACGCCCTCGGCCTGCCGAACCTCGTGGCGGCCATCCTCGCCGTCCTCACCTGCGTG
CTGCTGACGGGCGCCCTGCACGAGGATGGCCTCGCCGACACGGCGGACGGTTTCGGCGGTGGCGCGAGCCGCCTGCGCCG
CCTTGAAATCATGCGCGACAGCCGCATCGGCTCCTATGGCGCCTGCGCGCTCGTCTTCTCTCTGCTGCTGCGCGTCGCGC
TGCTGGATGGCCTGCTCGCCCTCTCCCCGACCCGCGCCGCCCTCGCGCTCATTGCCGCCGCGAGCCTGTCGCGGGCGGCG
GGGATGCTGCTGCTGGAATTCCTGCCGCCCGCACGCGCCGATGGGGCCTCGGCCGCCGCCGGCCAGCCCGGCGCGGACGC
GGCGGTGCGCTCCGCCCTCGTGGGCGCCCTCCTCGCCACCCTGCTGATCGTGCCGAGCACCGGGGTCGGTGCCACGCTGA
TGGCGATCGGGCTCTCCGTGCTCGCTCTCTTTGCCATGACGCGGCTCTCCAACCGCCTGATCGGCGGCCAGACGGGGGAT
GTGGCCGGCGCGGTCCAGCAACTCTGTGAGATTGCCTTCCTCATGGGCGTGCTTATCTATGCAAGGCCGCACTGA

Upstream 100 bases:

>100_bases
CCTGTGGGCGGCGTTTGATGCACTTGACCCGGTGCGGAAATAAGGCTCCCAAGATGGCCGCCCGTTCCAAGGGGGAGCGG
CGGCACGGAACGATGGACCC

Downstream 100 bases:

>100_bases
AGCAGTCCCATGACCGAGAGCCAGAGCCCCATCGCCTCCCCCTGCATCAAGGTCTGCGTCGTCGATCCGCTCACCAGCGT
GTGCGTGGGCTGCGGGCGCA

Product: cobalamin-5-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRHSRILADVGAALRFYSRIPVPAGAAEDAFAPPDLKRIAYAVPLAGAAIGLAGAAILIGAHALGLPNLVAAILAVLTCV
LLTGALHEDGLADTADGFGGGASRLRRLEIMRDSRIGSYGACALVFSLLLRVALLDGLLALSPTRAALALIAAASLSRAA
GMLLLEFLPPARADGASAAAGQPGADAAVRSALVGALLATLLIVPSTGVGATLMAIGLSVLALFAMTRLSNRLIGGQTGD
VAGAVQQLCEIAFLMGVLIYARPH

Sequences:

>Translated_264_residues
MRHSRILADVGAALRFYSRIPVPAGAAEDAFAPPDLKRIAYAVPLAGAAIGLAGAAILIGAHALGLPNLVAAILAVLTCV
LLTGALHEDGLADTADGFGGGASRLRRLEIMRDSRIGSYGACALVFSLLLRVALLDGLLALSPTRAALALIAAASLSRAA
GMLLLEFLPPARADGASAAAGQPGADAAVRSALVGALLATLLIVPSTGVGATLMAIGLSVLALFAMTRLSNRLIGGQTGD
VAGAVQQLCEIAFLMGVLIYARPH
>Mature_264_residues
MRHSRILADVGAALRFYSRIPVPAGAAEDAFAPPDLKRIAYAVPLAGAAIGLAGAAILIGAHALGLPNLVAAILAVLTCV
LLTGALHEDGLADTADGFGGGASRLRRLEIMRDSRIGSYGACALVFSLLLRVALLDGLLALSPTRAALALIAAASLSRAA
GMLLLEFLPPARADGASAAAGQPGADAAVRSALVGALLATLLIVPSTGVGATLMAIGLSVLALFAMTRLSNRLIGGQTGD
VAGAVQQLCEIAFLMGVLIYARPH

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=34.9397590361446, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_AZOC5 (A8ID91)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525812.1
- GeneID:   5691715
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2896
- HOGENOM:   HBG729392
- OMA:   WICLLEL
- ProtClustDB:   CLSK2516675
- BioCyc:   ACAU438753:AZC_2896-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26541; Mature: 26541

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2076ede8)-; HASH(0x21821e84)-; HASH(0x21e37928)-; HASH(0x1fd74b50)-; HASH(0x213ea50c)-; HASH(0x21e0a1f4)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSRILADVGAALRFYSRIPVPAGAAEDAFAPPDLKRIAYAVPLAGAAIGLAGAAILIG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AHALGLPNLVAAILAVLTCVLLTGALHEDGLADTADGFGGGASRLRRLEIMRDSRIGSYG
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
ACALVFSLLLRVALLDGLLALSPTRAALALIAAASLSRAAGMLLLEFLPPARADGASAAA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQPGADAAVRSALVGALLATLLIVPSTGVGATLMAIGLSVLALFAMTRLSNRLIGGQTGD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VAGAVQQLCEIAFLMGVLIYARPH
HHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MRHSRILADVGAALRFYSRIPVPAGAAEDAFAPPDLKRIAYAVPLAGAAIGLAGAAILIG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AHALGLPNLVAAILAVLTCVLLTGALHEDGLADTADGFGGGASRLRRLEIMRDSRIGSYG
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
ACALVFSLLLRVALLDGLLALSPTRAALALIAAASLSRAAGMLLLEFLPPARADGASAAA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQPGADAAVRSALVGALLATLLIVPSTGVGATLMAIGLSVLALFAMTRLSNRLIGGQTGD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VAGAVQQLCEIAFLMGVLIYARPH
HHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA