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The map label for this gene is mhpA [H]

Identifier: 158424506

GI number: 158424506

Start: 3283841

End: 3285367

Strand: Direct

Name: mhpA [H]

Synonym: AZC_2882

Alternate gene names: 158424506

Gene position: 3283841-3285367 (Clockwise)

Preceding gene: 158424504

Following gene: 158424507

Centisome position: 61.15

GC content: 71.25

Gene sequence:

>1527_bases
ATGCTCGAAGCCACCGATACGTCCGAAAACCCGTTCGACATCGTCATCATCGGCCTCGGTCCGGTCGGCGCGACGCTGGC
CAACCTGCTCGGCCTCAAGGGCCTCTCGACCCTCGTGCTGGAGCGCGAGAGCGAGGCCTACCACCTGCCGCGCGCGGTGC
ACTTCGACGATGAGGTCATGCGCGTGTTCCAGACCATCGGCCTCGCCGATGCGATCCTGCCGGCGACGCGCCTCAACCCC
GGCATGCGCTTCATCGATGCGCAGGGCGCGCTGCTCATGGACTGGCCCCGCCCGCAGGAGGTGAGCGGCCAGGGCTGGCA
TGCCAGCTACCGTTTCCACCAGCCGGATCTGGAGCGCATCCTGCGCGACGGCCTGCAGCGCTTCGCGAGCGTGACAGTGC
GCACCCGCTGCGATGCCTTCGCCTTCGAGCAGGATCGCGAGGGCGTCACCGTCCGCTATGAGGAACTGACCACCGGCCGC
CTGCGGCAGGTCCGTGCCCGCTATGTGGTCGGCTGCGACGGTGCCCGCTCGCTGGTGCGCCGTTTCATCGGGTCGTCCAT
GGACGATCTCGGCTTTCACGAGCGCTGGCTGGTGGTGGATGCCGTGCTGCGGCAGCCGAAGCCCGAGCTGGGCGACCACA
CCATCCAGTATTGCAACCCGGCGCGGCCGGCGACCTATGTGCGCGGCCCCGGCGACCGCCGGCGCTGGGAAATCACCGTG
CTGCCGCACGAGGACAGCCGCACGGTGGTCCAGCCGCAGACGGTCTGGTCGCTGCTCGCGCCGTGGCTCGCGCCCGACGA
GGCGGACCTGGAGCGGGCCGCCGTCTACACCTTCCATTCCGCCGTCGCCCGCCAGTGGCGGGCGGGCCGGCTGCTGCTCG
CGGGCGATGCCGCGCACCAGACGCCCCCCTTCATGGGTCAGGGCATGTGTTCGGGCATCCGCGACGTGGCCAATCTCGCC
TGGAAGCTGGAGGCGGTGGTGCACGGACAGGTCGGTGAGAGCCTGCTCGACACCTATCAGAGCGAACGCGGCCCGGACGC
CCATGCTTACATCGAAATGGCCGTGCGTCTGGGCGGGCTCATCAACACCTGCGGCACCGAAGCAGCCCTGAATGCCGCCT
TCCGCGACGCGGACGGCGTCGTCCGCATCCAGAGCCGCGTGCCCCGGCTGGGGCCGGGCATCGCTGCCGGCCGCAGCGAG
CTCGCCGGCAGCCGCTTCGCCCAGCCGAGGCTCGGCGACGGGCGGCTGCTGGATGACGCCGTCGGATTCAGCTTCGCCCT
GCTCGCCAGCGAGCGGATGTGGAGCGCCGCGAGCGAGCCGGTGCGCCGGCGCTTCGCGGCGGCCGGGGTGACGGTGGTCA
CCGAAGCCACCTGTCCGGCTCTGAAGCCCCTCCTGGACGCGCAAGGCGTCGGCGCCCTGCTGCTGCGGCCGGACCGCTAT
GTGGCGGGAGCGGCGCTCGATGACGGAGACCTTGCCGCCCTGGCCGACTGGCCGATGGTCTACCTGCCCCAACGCGCCGC
CGCCTGA

Upstream 100 bases:

>100_bases
GCCCGTATTGAAGGCCCCCGCCCGCTCTGGGATCAGTCGGCCCCAACAGGCACCAGTACCAAGGCGCCGCAGCAAGACGC
CAACAATCAAGGAGGGGACG

Downstream 100 bases:

>100_bases
TCCGGCCGGCACCGCCCGCCCGGCCCTCTCCTGTTTCCCCCCAAGAAGATGTTCAAGGAAAAGGTTCGCCCGATGAAGTT
CGCTTCCTTCACCCATGCCG

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: NA

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MLEATDTSENPFDIVIIGLGPVGATLANLLGLKGLSTLVLERESEAYHLPRAVHFDDEVMRVFQTIGLADAILPATRLNP
GMRFIDAQGALLMDWPRPQEVSGQGWHASYRFHQPDLERILRDGLQRFASVTVRTRCDAFAFEQDREGVTVRYEELTTGR
LRQVRARYVVGCDGARSLVRRFIGSSMDDLGFHERWLVVDAVLRQPKPELGDHTIQYCNPARPATYVRGPGDRRRWEITV
LPHEDSRTVVQPQTVWSLLAPWLAPDEADLERAAVYTFHSAVARQWRAGRLLLAGDAAHQTPPFMGQGMCSGIRDVANLA
WKLEAVVHGQVGESLLDTYQSERGPDAHAYIEMAVRLGGLINTCGTEAALNAAFRDADGVVRIQSRVPRLGPGIAAGRSE
LAGSRFAQPRLGDGRLLDDAVGFSFALLASERMWSAASEPVRRRFAAAGVTVVTEATCPALKPLLDAQGVGALLLRPDRY
VAGAALDDGDLAALADWPMVYLPQRAAA

Sequences:

>Translated_508_residues
MLEATDTSENPFDIVIIGLGPVGATLANLLGLKGLSTLVLERESEAYHLPRAVHFDDEVMRVFQTIGLADAILPATRLNP
GMRFIDAQGALLMDWPRPQEVSGQGWHASYRFHQPDLERILRDGLQRFASVTVRTRCDAFAFEQDREGVTVRYEELTTGR
LRQVRARYVVGCDGARSLVRRFIGSSMDDLGFHERWLVVDAVLRQPKPELGDHTIQYCNPARPATYVRGPGDRRRWEITV
LPHEDSRTVVQPQTVWSLLAPWLAPDEADLERAAVYTFHSAVARQWRAGRLLLAGDAAHQTPPFMGQGMCSGIRDVANLA
WKLEAVVHGQVGESLLDTYQSERGPDAHAYIEMAVRLGGLINTCGTEAALNAAFRDADGVVRIQSRVPRLGPGIAAGRSE
LAGSRFAQPRLGDGRLLDDAVGFSFALLASERMWSAASEPVRRRFAAAGVTVVTEATCPALKPLLDAQGVGALLLRPDRY
VAGAALDDGDLAALADWPMVYLPQRAAA
>Mature_508_residues
MLEATDTSENPFDIVIIGLGPVGATLANLLGLKGLSTLVLERESEAYHLPRAVHFDDEVMRVFQTIGLADAILPATRLNP
GMRFIDAQGALLMDWPRPQEVSGQGWHASYRFHQPDLERILRDGLQRFASVTVRTRCDAFAFEQDREGVTVRYEELTTGR
LRQVRARYVVGCDGARSLVRRFIGSSMDDLGFHERWLVVDAVLRQPKPELGDHTIQYCNPARPATYVRGPGDRRRWEITV
LPHEDSRTVVQPQTVWSLLAPWLAPDEADLERAAVYTFHSAVARQWRAGRLLLAGDAAHQTPPFMGQGMCSGIRDVANLA
WKLEAVVHGQVGESLLDTYQSERGPDAHAYIEMAVRLGGLINTCGTEAALNAAFRDADGVVRIQSRVPRLGPGIAAGRSE
LAGSRFAQPRLGDGRLLDDAVGFSFALLASERMWSAASEPVRRRFAAAGVTVVTEATCPALKPLLDAQGVGALLLRPDRY
VAGAALDDGDLAALADWPMVYLPQRAAA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=519, Percent_Identity=32.5626204238921, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 55671; Mature: 55671

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEATDTSENPFDIVIIGLGPVGATLANLLGLKGLSTLVLERESEAYHLPRAVHFDDEVM
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHEEECCCCHHCCCEEECCCHHHH
RVFQTIGLADAILPATRLNPGMRFIDAQGALLMDWPRPQEVSGQGWHASYRFHQPDLERI
HHHHHHHHHHHHCCHHHCCCCCEEEECCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHH
LRDGLQRFASVTVRTRCDAFAFEQDREGVTVRYEELTTGRLRQVRARYVVGCDGARSLVR
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHEEECCCHHHHHHH
RFIGSSMDDLGFHERWLVVDAVLRQPKPELGDHTIQYCNPARPATYVRGPGDRRRWEITV
HHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCCEEEEEE
LPHEDSRTVVQPQTVWSLLAPWLAPDEADLERAAVYTFHSAVARQWRAGRLLLAGDAAHQ
EECCCCCCEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPPFMGQGMCSGIRDVANLAWKLEAVVHGQVGESLLDTYQSERGPDAHAYIEMAVRLGGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
INTCGTEAALNAAFRDADGVVRIQSRVPRLGPGIAAGRSELAGSRFAQPRLGDGRLLDDA
HHHCCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEHHH
VGFSFALLASERMWSAASEPVRRRFAAAGVTVVTEATCPALKPLLDAQGVGALLLRPDRY
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCCEEEECCCCE
VAGAALDDGDLAALADWPMVYLPQRAAA
EECCCCCCCCEEEEECCCEEECCHHCCC
>Mature Secondary Structure
MLEATDTSENPFDIVIIGLGPVGATLANLLGLKGLSTLVLERESEAYHLPRAVHFDDEVM
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHEEECCCCHHCCCEEECCCHHHH
RVFQTIGLADAILPATRLNPGMRFIDAQGALLMDWPRPQEVSGQGWHASYRFHQPDLERI
HHHHHHHHHHHHCCHHHCCCCCEEEECCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHH
LRDGLQRFASVTVRTRCDAFAFEQDREGVTVRYEELTTGRLRQVRARYVVGCDGARSLVR
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHEEECCCHHHHHHH
RFIGSSMDDLGFHERWLVVDAVLRQPKPELGDHTIQYCNPARPATYVRGPGDRRRWEITV
HHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCCEEEEEE
LPHEDSRTVVQPQTVWSLLAPWLAPDEADLERAAVYTFHSAVARQWRAGRLLLAGDAAHQ
EECCCCCCEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
TPPFMGQGMCSGIRDVANLAWKLEAVVHGQVGESLLDTYQSERGPDAHAYIEMAVRLGGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
INTCGTEAALNAAFRDADGVVRIQSRVPRLGPGIAAGRSELAGSRFAQPRLGDGRLLDDA
HHHCCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEHHH
VGFSFALLASERMWSAASEPVRRRFAAAGVTVVTEATCPALKPLLDAQGVGALLLRPDRY
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCCEEEECCCCE
VAGAALDDGDLAALADWPMVYLPQRAAA
EECCCCCCCCEEEEECCCEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA