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The map label for this gene is lacI [H]

Identifier: 158424480

GI number: 158424480

Start: 3252448

End: 3253551

Strand: Reverse

Name: lacI [H]

Synonym: AZC_2856

Alternate gene names: 158424480

Gene position: 3253551-3252448 (Counterclockwise)

Preceding gene: 158424487

Following gene: 158424479

Centisome position: 60.59

GC content: 71.01

Gene sequence:

>1104_bases
GTGCATTCGCCTTGTCAATCGTTAACTGAGGGCACGGTGTTGCGGCGTGAGGGACGGGACGTGTTTCAGGAGGAGGAAAC
CCAGGAGCGGCCGGGGCGGATCACGATCCTTGACGTGGCCGCTGCGGCGGGTGTCTCCAAATCCACGGTCTCCCGCATCC
TGGACGAGCGCCTGCCGCGCTCCGAGAGCGAGACGGCGCGCCGCGTGCGGGAGGTGGCGGCCCGGCTCGGCTATGTGCGC
GACGTGTCCGCCGCCAGCCTCCGCCGCGGCCAGACCAACACCATTGGCGTGATCGTGCCGCGCCTGACCGATACGGTCAT
GGCTATGCTGTACGAGGCGCTGGCGCGGGCCTGTGCGCGCTCCGGCCGCTTCGCCATCGTGGCGACCACCGACGATGAGC
CCCGCGCCGACCGGGCAGCCGCGAAGAGCCTGCTCCAGCGCGGCGTGGACGGGCTCGTGCTGTCCACCGCCCGCGAGGGG
GACGATTTCGCGCAGGAACTGGCGGACCGCGGCGTGCCCTATGTCCTTGCCCTGCGCAGCGACGGCCGCAGCCTTGCCTC
CGTGGGCGACGACCGGCTGGGCGGCTATCTCGCCACCCGCCATCTCATCGATCTCGGCCACCGCCGCATCGGCATCATTG
CCGGGCCGAGCTACGCATCCAGCAGCCGCGGGCGGCTCGCCGGCTATCTCCAGGCCCTGGAAGAGCAGGGCGTGCCGCAC
GATCCGGCCCTGCTGGTGGAGTCCACCTTCGGCATCGATTCCGGTGCGGAGGCGGCCGAGCATCTGATGCGCCTGCCCGA
TCCGCCCACCGCCATCTTCGCCGTGAACGACAACACCGCCATCGGCGCGCTCTCCGCTCTGGCGCGGCTCGGCGTCTCGG
TGCCGGGGGACGTGTCCCTCGTCGGCTATAACGACATCCCCATCGTGAGCCGGCTGCCCATCCCGCTGACCTCCGTGCGG
GTGCCATTCGACCAGATCGCCACCTCCGCCCTCGACCTCATGACTCAGTGGCCGGACACGAAGGGCGAGCGCGTCCGCGT
CGCGGCGCCCACGCTCATCCCTCGCCGGTCGAGCGGTCCGCCGAACCGGCGAATCGGCGCCTGA

Upstream 100 bases:

>100_bases
GTCTCGTTCCTTCCCTAGGAGTGTCAGACCGAAATACGGGTCCCGCCTCCGCTCCCCCGTTTTTAGGATCGTTAGCGGGA
ACGTTCCCATCATCAGACTT

Downstream 100 bases:

>100_bases
GCCCGCCGGGTGCTTCCGCAGCGTCCTTCGCGCGGCGGGAGCAAGGGGCGATTTGTGGGGACAAGACGGCTTCGTGACCG
CGCGAGAGGGCTTGGTGGCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MHSPCQSLTEGTVLRREGRDVFQEEETQERPGRITILDVAAAAGVSKSTVSRILDERLPRSESETARRVREVAARLGYVR
DVSAASLRRGQTNTIGVIVPRLTDTVMAMLYEALARACARSGRFAIVATTDDEPRADRAAAKSLLQRGVDGLVLSTAREG
DDFAQELADRGVPYVLALRSDGRSLASVGDDRLGGYLATRHLIDLGHRRIGIIAGPSYASSSRGRLAGYLQALEEQGVPH
DPALLVESTFGIDSGAEAAEHLMRLPDPPTAIFAVNDNTAIGALSALARLGVSVPGDVSLVGYNDIPIVSRLPIPLTSVR
VPFDQIATSALDLMTQWPDTKGERVRVAAPTLIPRRSSGPPNRRIGA

Sequences:

>Translated_367_residues
MHSPCQSLTEGTVLRREGRDVFQEEETQERPGRITILDVAAAAGVSKSTVSRILDERLPRSESETARRVREVAARLGYVR
DVSAASLRRGQTNTIGVIVPRLTDTVMAMLYEALARACARSGRFAIVATTDDEPRADRAAAKSLLQRGVDGLVLSTAREG
DDFAQELADRGVPYVLALRSDGRSLASVGDDRLGGYLATRHLIDLGHRRIGIIAGPSYASSSRGRLAGYLQALEEQGVPH
DPALLVESTFGIDSGAEAAEHLMRLPDPPTAIFAVNDNTAIGALSALARLGVSVPGDVSLVGYNDIPIVSRLPIPLTSVR
VPFDQIATSALDLMTQWPDTKGERVRVAAPTLIPRRSSGPPNRRIGA
>Mature_367_residues
MHSPCQSLTEGTVLRREGRDVFQEEETQERPGRITILDVAAAAGVSKSTVSRILDERLPRSESETARRVREVAARLGYVR
DVSAASLRRGQTNTIGVIVPRLTDTVMAMLYEALARACARSGRFAIVATTDDEPRADRAAAKSLLQRGVDGLVLSTAREG
DDFAQELADRGVPYVLALRSDGRSLASVGDDRLGGYLATRHLIDLGHRRIGIIAGPSYASSSRGRLAGYLQALEEQGVPH
DPALLVESTFGIDSGAEAAEHLMRLPDPPTAIFAVNDNTAIGALSALARLGVSVPGDVSLVGYNDIPIVSRLPIPLTSVR
VPFDQIATSALDLMTQWPDTKGERVRVAAPTLIPRRSSGPPNRRIGA

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=33.7349397590361, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=31.0030395136778, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1790369, Length=303, Percent_Identity=31.6831683168317, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789202, Length=338, Percent_Identity=32.2485207100592, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788474, Length=311, Percent_Identity=32.475884244373, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787906, Length=320, Percent_Identity=32.1875, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789068, Length=300, Percent_Identity=31.6666666666667, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=28.3582089552239, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787580, Length=282, Percent_Identity=26.5957446808511, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994940, Length=303, Percent_Identity=27.3927392739274, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786268, Length=302, Percent_Identity=23.1788079470199, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790715, Length=303, Percent_Identity=24.4224422442244, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=25.0783699059561, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39311; Mature: 39311

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPCQSLTEGTVLRREGRDVFQEEETQERPGRITILDVAAAAGVSKSTVSRILDERLPR
CCCHHHHHHHCHHHHHCCHHHHHHHHHHCCCCEEEEEEEHHHCCCCHHHHHHHHHHHCCC
SESETARRVREVAARLGYVRDVSAASLRRGQTNTIGVIVPRLTDTVMAMLYEALARACAR
CHHHHHHHHHHHHHHHCCHHCCHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHC
SGRFAIVATTDDEPRADRAAAKSLLQRGVDGLVLSTAREGDDFAQELADRGVPYVLALRS
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECC
DGRSLASVGDDRLGGYLATRHLIDLGHRRIGIIAGPSYASSSRGRLAGYLQALEEQGVPH
CCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
DPALLVESTFGIDSGAEAAEHLMRLPDPPTAIFAVNDNTAIGALSALARLGVSVPGDVSL
CCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
VGYNDIPIVSRLPIPLTSVRVPFDQIATSALDLMTQWPDTKGERVRVAAPTLIPRRSSGP
EECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
PNRRIGA
CCCCCCC
>Mature Secondary Structure
MHSPCQSLTEGTVLRREGRDVFQEEETQERPGRITILDVAAAAGVSKSTVSRILDERLPR
CCCHHHHHHHCHHHHHCCHHHHHHHHHHCCCCEEEEEEEHHHCCCCHHHHHHHHHHHCCC
SESETARRVREVAARLGYVRDVSAASLRRGQTNTIGVIVPRLTDTVMAMLYEALARACAR
CHHHHHHHHHHHHHHHCCHHCCHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHC
SGRFAIVATTDDEPRADRAAAKSLLQRGVDGLVLSTAREGDDFAQELADRGVPYVLALRS
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECC
DGRSLASVGDDRLGGYLATRHLIDLGHRRIGIIAGPSYASSSRGRLAGYLQALEEQGVPH
CCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
DPALLVESTFGIDSGAEAAEHLMRLPDPPTAIFAVNDNTAIGALSALARLGVSVPGDVSL
CCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
VGYNDIPIVSRLPIPLTSVRVPFDQIATSALDLMTQWPDTKGERVRVAAPTLIPRRSSGP
EECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
PNRRIGA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]