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The map label for this gene is gatB

Identifier: 158424471

GI number: 158424471

Start: 3240630

End: 3242108

Strand: Direct

Name: gatB

Synonym: AZC_2847

Alternate gene names: 158424471

Gene position: 3240630-3242108 (Clockwise)

Preceding gene: 158424469

Following gene: 158424476

Centisome position: 60.35

GC content: 65.52

Gene sequence:

>1479_bases
ATGACCGCCCACGTCCGACCGACCGACGCCAAGAAGCTCATCAAGGGCGCCACCGGCAATTGGGAAGTGGTGATTGGCAT
GGAAATCCATGCCCAGGTCTCGTCCAACGCGAAGCTGTTCTCCGGCGCCTCCACCGCCTTCGGCGGCCCGCCGAACGACC
ATGTGTCGCTGGTGGACGCGGCCATGCCGGGCATGCTGCCGGTCATCAACAAGGAATGCGTGGCGCAGGCGGTGCGCACC
GGCCTCGGTCTCCGGGCGCAGATCAACCTGCGCTCCACCTTCGACCGCAAGAACTACTTCTATCCGGACCTGCCGCAGGG
CTACCAGATCAGCCAGTACAAGAGCCCCATCGTGGGTGAAGGCGAGGTGATCGTGGACCTGCTGGACGGCGAGAGCTTCG
TCGTCGGCATCGAGCGCCTGCATCTGGAGCAGGATGCCGGCAAGTCTATCCACGACCTGTCCCCCACCCTCTCCTTCGTG
GACCTCAACCGCTCCGGCGTGGCCCTCATGGAGATCGTGTCCCGCCCCGACCTGCGCTCGTCGGAGGAGGCCCGCGCCTA
TGTGACCAAGCTGCGCTCCATCCTGCGCTATCTCGGCACCTGCGACGGCGACATGGAGAAGGGCAACCTGCGCGCCGACG
TGAACGTCTCCGTGCGCCGCCCCGGCGAGCCGCTGGGCACGCGCTGCGAGATCAAGAACGTCAATTCCATCCGCTTCATC
GGCCAGGCCATCGAGGTGGAGGCCCGGCGCCAGATCGAGATCCTGGAGGATGGCGGCGTCATCGATCAGGAAACCCGCCT
GTTCGACCCCAACAAGGGCGAGACGCGCTCCATGCGCTCCAAGGAAGAGGCGCACGATTATCGCTACTTCCCCGATCCGG
ACCTGCTGCCCCTCGTGCTGCATGCCGATTTCGTGGAGGAGTTGAAGTCGCACCTTCCCGAGCTGCCGGACGAGAAGAAG
GCGCGCTTCATCGCCGACTACGGCCTCAGCCCCTATGACGCGAGCGTGCTGGTGGCCGAGCGCGACACGGCGGACTATTT
CGAGCAGGTGGCCAAGGGCCGCGACGGCAAGGCGGCGGCGAACTTCGTCATCAACGAGCTGTTCGGCCGCCTGAACAAGG
ACGGCAAGGACGTCATCACCTCGCCCGTCTCCCCGGCGCAGGTGGCCGCCATCGTCGATCTCATCGGCGAGGGCGTCATC
TCCTCCAAGATCGCCAAGGACCTGTTCGAGATCGTCTATACGGAAGGCGGCGATCCGCGCGTGCTCGTGGAGGAGCGCGG
CCTGAAGCAGGTGACGGACACCGGCGCCATCGAAAAGGTGGTGGATGAGATCATCGCCGCCAATCCCGACAAGGTGGCGC
AGGTGCAGGCCAAGCCCACCATGCTGGGCTGGTTCGTCGGCCAGGCCATGAAGGCCTCCGGCGGCAAGGCCAACCCGCAG
GCCCTCAACGACATCCTGAAGCGCAAGCTCGGCATCTGA

Upstream 100 bases:

>100_bases
CTAGAGCGCGAACGGGTTGTGTCAATCGCTGCGGTTGCCACGCTTTGCCGGGGGGAGTAGGGGACAGGGCAGACCGTTTT
CACATCCGCAAGGGATAGCG

Downstream 100 bases:

>100_bases
GCGGCGCGTCCTAGACAGTGAGACACCGGCCTTCGGGCCGGTGTTTTCGTTTGGAGGCCCGGAGAGCATCCGACGCCCTC
CCCGGCCTCACATGAGGGCG

Product: glutamyl-tRNA(Gln) amidotransferase B subunit

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTAHVRPTDAKKLIKGATGNWEVVIGMEIHAQVSSNAKLFSGASTAFGGPPNDHVSLVDAAMPGMLPVINKECVAQAVRT
GLGLRAQINLRSTFDRKNYFYPDLPQGYQISQYKSPIVGEGEVIVDLLDGESFVVGIERLHLEQDAGKSIHDLSPTLSFV
DLNRSGVALMEIVSRPDLRSSEEARAYVTKLRSILRYLGTCDGDMEKGNLRADVNVSVRRPGEPLGTRCEIKNVNSIRFI
GQAIEVEARRQIEILEDGGVIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLVLHADFVEELKSHLPELPDEKK
ARFIADYGLSPYDASVLVAERDTADYFEQVAKGRDGKAAANFVINELFGRLNKDGKDVITSPVSPAQVAAIVDLIGEGVI
SSKIAKDLFEIVYTEGGDPRVLVEERGLKQVTDTGAIEKVVDEIIAANPDKVAQVQAKPTMLGWFVGQAMKASGGKANPQ
ALNDILKRKLGI

Sequences:

>Translated_492_residues
MTAHVRPTDAKKLIKGATGNWEVVIGMEIHAQVSSNAKLFSGASTAFGGPPNDHVSLVDAAMPGMLPVINKECVAQAVRT
GLGLRAQINLRSTFDRKNYFYPDLPQGYQISQYKSPIVGEGEVIVDLLDGESFVVGIERLHLEQDAGKSIHDLSPTLSFV
DLNRSGVALMEIVSRPDLRSSEEARAYVTKLRSILRYLGTCDGDMEKGNLRADVNVSVRRPGEPLGTRCEIKNVNSIRFI
GQAIEVEARRQIEILEDGGVIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLVLHADFVEELKSHLPELPDEKK
ARFIADYGLSPYDASVLVAERDTADYFEQVAKGRDGKAAANFVINELFGRLNKDGKDVITSPVSPAQVAAIVDLIGEGVI
SSKIAKDLFEIVYTEGGDPRVLVEERGLKQVTDTGAIEKVVDEIIAANPDKVAQVQAKPTMLGWFVGQAMKASGGKANPQ
ALNDILKRKLGI
>Mature_491_residues
TAHVRPTDAKKLIKGATGNWEVVIGMEIHAQVSSNAKLFSGASTAFGGPPNDHVSLVDAAMPGMLPVINKECVAQAVRTG
LGLRAQINLRSTFDRKNYFYPDLPQGYQISQYKSPIVGEGEVIVDLLDGESFVVGIERLHLEQDAGKSIHDLSPTLSFVD
LNRSGVALMEIVSRPDLRSSEEARAYVTKLRSILRYLGTCDGDMEKGNLRADVNVSVRRPGEPLGTRCEIKNVNSIRFIG
QAIEVEARRQIEILEDGGVIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLVLHADFVEELKSHLPELPDEKKA
RFIADYGLSPYDASVLVAERDTADYFEQVAKGRDGKAAANFVINELFGRLNKDGKDVITSPVSPAQVAAIVDLIGEGVIS
SKIAKDLFEIVYTEGGDPRVLVEERGLKQVTDTGAIEKVVDEIIAANPDKVAQVQAKPTMLGWFVGQAMKASGGKANPQA
LNDILKRKLGI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=506, Percent_Identity=41.501976284585, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI115534094, Length=309, Percent_Identity=43.3656957928803, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=524, Percent_Identity=31.4885496183206, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=37.525354969574, Blast_Score=325, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_AZOC5 (A8ICV0)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525763.1
- ProteinModelPortal:   A8ICV0
- SMR:   A8ICV0
- GeneID:   5689401
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2847
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   CLSK980142
- BioCyc:   ACAU438753:AZC_2847-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53863; Mature: 53732

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHVRPTDAKKLIKGATGNWEVVIGMEIHAQVSSNAKLFSGASTAFGGPPNDHVSLVDA
CCCCCCCCCHHHHHHCCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEHHHH
AMPGMLPVINKECVAQAVRTGLGLRAQINLRSTFDRKNYFYPDLPQGYQISQYKSPIVGE
HCCCCCHHHCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
GEVIVDLLDGESFVVGIERLHLEQDAGKSIHDLSPTLSFVDLNRSGVALMEIVSRPDLRS
CCEEEEEECCCEEEEEHHHHHHHHHCCCCHHHCCCCEEEEECCCCCHHHHHHHCCCCCCC
SEEARAYVTKLRSILRYLGTCDGDMEKGNLRADVNVSVRRPGEPLGTRCEIKNVNSIRFI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHH
GQAIEVEARRQIEILEDGGVIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLVL
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
HADFVEELKSHLPELPDEKKARFIADYGLSPYDASVLVAERDTADYFEQVAKGRDGKAAA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCHHHH
NFVINELFGRLNKDGKDVITSPVSPAQVAAIVDLIGEGVISSKIAKDLFEIVYTEGGDPR
HHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCE
VLVEERGLKQVTDTGAIEKVVDEIIAANPDKVAQVQAKPTMLGWFVGQAMKASGGKANPQ
EEEECCCCHHHHCCHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHHHHHHHCCCCCCCHH
ALNDILKRKLGI
HHHHHHHHHHCC
>Mature Secondary Structure 
TAHVRPTDAKKLIKGATGNWEVVIGMEIHAQVSSNAKLFSGASTAFGGPPNDHVSLVDA
CCCCCCCCHHHHHHCCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEHHHH
AMPGMLPVINKECVAQAVRTGLGLRAQINLRSTFDRKNYFYPDLPQGYQISQYKSPIVGE
HCCCCCHHHCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
GEVIVDLLDGESFVVGIERLHLEQDAGKSIHDLSPTLSFVDLNRSGVALMEIVSRPDLRS
CCEEEEEECCCEEEEEHHHHHHHHHCCCCHHHCCCCEEEEECCCCCHHHHHHHCCCCCCC
SEEARAYVTKLRSILRYLGTCDGDMEKGNLRADVNVSVRRPGEPLGTRCEIKNVNSIRFI
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHH
GQAIEVEARRQIEILEDGGVIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLVL
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
HADFVEELKSHLPELPDEKKARFIADYGLSPYDASVLVAERDTADYFEQVAKGRDGKAAA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCHHHH
NFVINELFGRLNKDGKDVITSPVSPAQVAAIVDLIGEGVISSKIAKDLFEIVYTEGGDPR
HHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCE
VLVEERGLKQVTDTGAIEKVVDEIIAANPDKVAQVQAKPTMLGWFVGQAMKASGGKANPQ
EEEECCCCHHHHCCHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHHHHHHHCCCCCCCHH
ALNDILKRKLGI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA