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The map label for this gene is phrB [H]

Identifier: 158424448

GI number: 158424448

Start: 3214405

End: 3215859

Strand: Direct

Name: phrB [H]

Synonym: AZC_2824

Alternate gene names: 158424448

Gene position: 3214405-3215859 (Clockwise)

Preceding gene: 158424447

Following gene: 158424449

Centisome position: 59.86

GC content: 71.48

Gene sequence:

>1455_bases
ATGAGCGCCGCCGGCACCGCCGTCATCTGGTTCCGAGACGACCTGCGCCTTGCGGACAATCCCGCGCTCGCCGCCGCGGC
CGCCAGCGGGCGGCGGGTCCTCGCGCTTTATGTGCTGGACGAGGACAGCCCCGGCGTCCGCGCGCTCGGCGGCGCCGCGC
TCTGGTGGCTGGCGGGATCGCTGCGGGCGCTGAAGGCGGAACTCGGCCAGCATGGCGTGCCCCTCGTGCTGCGGCGGGGG
GAGGCCGGGCGCATCGTGCCGGACGTGGCGCGTGCCGCGGGTGCCGACCTCGTCGCCTTCAACTGGCGGGCGGGCAAGGC
GGAGCAGGCGGTGGACGAGGCGGTGGCCCGCCATCTTTCGGCCGCCGGCGTGACGGTGGAGCGCACGCTCGGCCATCTGC
TCCACGCGCCCGGCAGCGTGCGGGGCACGGGCGGCGGCCTGCCGCGCACCTTCTCCTCCTTCATGCGGGCGGCGGTGAAG
GGGCGCGACATCGGCCGCACCCTGCCCGTTCCGCGCCTCAACGGCGCCGCGCCCCTGCCCTGCGATGATCTGGAGGAGTG
GGCGCTTGAGCCGACACAGCCCGACTGGGCCGGCGGACTGCGCACCACATGGCATCCCGGCGAAGCGGCGGCGCGAGCCC
GGCTCGAAGCCTTCCTCGCCGATGGTCTTGCCGGCTATGGCGAAGGCCGCGACCGGCCGGACCAGACCCATGTGTCGCGC
CTTTCGCCCTATCTGCGCTTCGGGGAGATCTCGCCCCGGCAGGTGCTGCTGGCGGCGCTCGCCGCGGGCGATGCGGGCAC
CGTCCCGGCCAAGGACGTGCAGAAGTTCGAGGCCGAGCTCTACTGGCGGGAATTCTCCCACCATCTCCTCTTTGCGGTGC
CGGACCTCGCGCGCCGCAACCTGCAAGCCTCCTTCGACGCCTTCCCGTGGCGCGAATGCCCCGGCGACCTCAAGAAATGG
CAGAAGGGCCGCACCGGCTATCCCATCGTGGACGCGGGCATGCGCCAGCTCTGGCAGACCGGCTGGATGCACAATCGCGT
GCGCATGATCGTCGCCTCCTTCCTCTGCAAGCACCTCCTGTTGCCCTGGCAGGATGGCGAACTGTGGTTCTGGGACACGC
TGGTGGATGCGGACCTCGCCAACAATGCCGCAAGCTGGCAGTGGGTGGCGGGCTCGGGCGCCGACGCCGCGCCCTATTTC
CGCATCTTCAATCCGGTGCTTCAGGGCGAGAAATTCGATCCCGAGGGCACCTATGTGCGCACCTATGTGCCCGAGCTGAA
GGACGTGCCCGCCCGCCTCATCCACCAGCCCTGGAGCGCGCCGCTGGAGTTGACGCGATACGGCGTGCGGCTCGGCCGCG
ACTATCCCGAGCCCATGGTGGAGCATGGCGCCGCCCGCGACAGAGCGCTGCGGGCATTCGAGCATACCCGCAAGGATCTG
CGCTCGGCGGGCTGA

Upstream 100 bases:

>100_bases
GTTACCATCCTCCGTCCGCGAGGCGTATGATCCTCCCGTCTGGTCCGCGCGTAATCGCCTCGGGTCCGCGCGCGGCTGGC
CCGGAAGAGGGAGAACAGCC

Downstream 100 bases:

>100_bases
TCCCGCCGCCCCGCCCCTCCTCTCTTTCAAAGCTCCCCGCGCCACGGGACCGGCGGCGCGGTTGCCGCCGCCTGCCCCCT
TGGCTAGATTGCACGGAACA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSAAGTAVIWFRDDLRLADNPALAAAAASGRRVLALYVLDEDSPGVRALGGAALWWLAGSLRALKAELGQHGVPLVLRRG
EAGRIVPDVARAAGADLVAFNWRAGKAEQAVDEAVARHLSAAGVTVERTLGHLLHAPGSVRGTGGGLPRTFSSFMRAAVK
GRDIGRTLPVPRLNGAAPLPCDDLEEWALEPTQPDWAGGLRTTWHPGEAAARARLEAFLADGLAGYGEGRDRPDQTHVSR
LSPYLRFGEISPRQVLLAALAAGDAGTVPAKDVQKFEAELYWREFSHHLLFAVPDLARRNLQASFDAFPWRECPGDLKKW
QKGRTGYPIVDAGMRQLWQTGWMHNRVRMIVASFLCKHLLLPWQDGELWFWDTLVDADLANNAASWQWVAGSGADAAPYF
RIFNPVLQGEKFDPEGTYVRTYVPELKDVPARLIHQPWSAPLELTRYGVRLGRDYPEPMVEHGAARDRALRAFEHTRKDL
RSAG

Sequences:

>Translated_484_residues
MSAAGTAVIWFRDDLRLADNPALAAAAASGRRVLALYVLDEDSPGVRALGGAALWWLAGSLRALKAELGQHGVPLVLRRG
EAGRIVPDVARAAGADLVAFNWRAGKAEQAVDEAVARHLSAAGVTVERTLGHLLHAPGSVRGTGGGLPRTFSSFMRAAVK
GRDIGRTLPVPRLNGAAPLPCDDLEEWALEPTQPDWAGGLRTTWHPGEAAARARLEAFLADGLAGYGEGRDRPDQTHVSR
LSPYLRFGEISPRQVLLAALAAGDAGTVPAKDVQKFEAELYWREFSHHLLFAVPDLARRNLQASFDAFPWRECPGDLKKW
QKGRTGYPIVDAGMRQLWQTGWMHNRVRMIVASFLCKHLLLPWQDGELWFWDTLVDADLANNAASWQWVAGSGADAAPYF
RIFNPVLQGEKFDPEGTYVRTYVPELKDVPARLIHQPWSAPLELTRYGVRLGRDYPEPMVEHGAARDRALRAFEHTRKDL
RSAG
>Mature_483_residues
SAAGTAVIWFRDDLRLADNPALAAAAASGRRVLALYVLDEDSPGVRALGGAALWWLAGSLRALKAELGQHGVPLVLRRGE
AGRIVPDVARAAGADLVAFNWRAGKAEQAVDEAVARHLSAAGVTVERTLGHLLHAPGSVRGTGGGLPRTFSSFMRAAVKG
RDIGRTLPVPRLNGAAPLPCDDLEEWALEPTQPDWAGGLRTTWHPGEAAARARLEAFLADGLAGYGEGRDRPDQTHVSRL
SPYLRFGEISPRQVLLAALAAGDAGTVPAKDVQKFEAELYWREFSHHLLFAVPDLARRNLQASFDAFPWRECPGDLKKWQ
KGRTGYPIVDAGMRQLWQTGWMHNRVRMIVASFLCKHLLLPWQDGELWFWDTLVDADLANNAASWQWVAGSGADAAPYFR
IFNPVLQGEKFDPEGTYVRTYVPELKDVPARLIHQPWSAPLELTRYGVRLGRDYPEPMVEHGAARDRALRAFEHTRKDLR
SAG

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=491, Percent_Identity=29.5315682281059, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI188536100, Length=491, Percent_Identity=29.7352342158859, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI188536103, Length=428, Percent_Identity=29.6728971962617, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1786926, Length=499, Percent_Identity=38.6773547094188, Blast_Score=294, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6324962, Length=515, Percent_Identity=29.126213592233, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI17137248, Length=505, Percent_Identity=32.6732673267327, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24585455, Length=505, Percent_Identity=32.6732673267327, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24648152, Length=522, Percent_Identity=29.1187739463602, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53163; Mature: 53032

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAGTAVIWFRDDLRLADNPALAAAAASGRRVLALYVLDEDSPGVRALGGAALWWLAGS
CCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
LRALKAELGQHGVPLVLRRGEAGRIVPDVARAAGADLVAFNWRAGKAEQAVDEAVARHLS
HHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
AAGVTVERTLGHLLHAPGSVRGTGGGLPRTFSSFMRAAVKGRDIGRTLPVPRLNGAAPLP
HCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
CDDLEEWALEPTQPDWAGGLRTTWHPGEAAARARLEAFLADGLAGYGEGRDRPDQTHVSR
CCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHH
LSPYLRFGEISPRQVLLAALAAGDAGTVPAKDVQKFEAELYWREFSHHLLFAVPDLARRN
HCHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHH
LQASFDAFPWRECPGDLKKWQKGRTGYPIVDAGMRQLWQTGWMHNRVRMIVASFLCKHLL
HHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
LPWQDGELWFWDTLVDADLANNAASWQWVAGSGADAAPYFRIFNPVLQGEKFDPEGTYVR
CCCCCCCEEEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
TYVPELKDVPARLIHQPWSAPLELTRYGVRLGRDYPEPMVEHGAARDRALRAFEHTRKDL
EECCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
RSAG
HCCC
>Mature Secondary Structure 
SAAGTAVIWFRDDLRLADNPALAAAAASGRRVLALYVLDEDSPGVRALGGAALWWLAGS
CCCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
LRALKAELGQHGVPLVLRRGEAGRIVPDVARAAGADLVAFNWRAGKAEQAVDEAVARHLS
HHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
AAGVTVERTLGHLLHAPGSVRGTGGGLPRTFSSFMRAAVKGRDIGRTLPVPRLNGAAPLP
HCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
CDDLEEWALEPTQPDWAGGLRTTWHPGEAAARARLEAFLADGLAGYGEGRDRPDQTHVSR
CCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHH
LSPYLRFGEISPRQVLLAALAAGDAGTVPAKDVQKFEAELYWREFSHHLLFAVPDLARRN
HCHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHH
LQASFDAFPWRECPGDLKKWQKGRTGYPIVDAGMRQLWQTGWMHNRVRMIVASFLCKHLL
HHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
LPWQDGELWFWDTLVDADLANNAASWQWVAGSGADAAPYFRIFNPVLQGEKFDPEGTYVR
CCCCCCCEEEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
TYVPELKDVPARLIHQPWSAPLELTRYGVRLGRDYPEPMVEHGAARDRALRAFEHTRKDL
EECCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
RSAG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]