Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 158424436

GI number: 158424436

Start: 3197468

End: 3198256

Strand: Direct

Name: xthA [H]

Synonym: AZC_2812

Alternate gene names: 158424436

Gene position: 3197468-3198256 (Clockwise)

Preceding gene: 158424433

Following gene: 158424444

Centisome position: 59.55

GC content: 66.79

Gene sequence:

>789_bases
ATGCGTATCGCCACCTGGAACGTGAATTCCGTCCGCCTGCGCACCGAACATGCCGTCGCCTGGCTGAAGGAGGCACAGCC
GGACGTGGTGTGCCTGCAGGAGATCAAGTGCCAGACCGAGCAGTTCCCCCGCGAGCCCTTCGAGGCGCTGGGCTACAATG
TGGCGGTGCATGGACAGAAGGGCTTCAACGGCGTGGCCATCCTCTCCAAGCGCCCGCTGGAGGACGTGACCAACGGACTT
GCTGGCGACGACAGCGACGTGCAGGCCCGCTTCATCGAGGCGGTGGTTTCGGTGCCCGGCGGGGTGGTGCGGGTGTGCTG
CATCTATTTGCCCAACGGCAACCCGCCGCAGACCGAGAAATATGACTACAAGCTCGGCTTCATGGCCCGGCTGAAGGCCT
TCACCGAAAGCCGCCTGAAGCTGGAGGAGCCGTTGATCCTCGCCGGCGACTTCAACGTGATTCCCGATGCCCGCGATGCC
GCCGATCCCGCCGCCTGGGTCGAGGATGCGCTCTATCTGCCGCGCACCCGCGCCGCCTTCCGCGAGATCATCAACCTCGG
CATGACGGACGCGCTGCGCGCCACGTCCGACGCCGAGCGCCTCTTCACCTTCTGGGATTATCAGGCGGGCGCCTGGCAGC
GGGACTTCGGCATCCGCATCGATCACCTGCTGCTCTCGCCGCAGGCGGCCGACCGCCTGATCGGCGCCGGCATCGACAAG
CACCTGCGCGCCTTGGAGCGCCCCTCGGACCACGTGCCCGCCTGGATCGACCTGAAGATGGCGGCGTGA

Upstream 100 bases:

>100_bases
CTCTGTCTGGAACATTTCGAGCAATGATTTCTGTTCCGTTGCGGCGCCCCTCCCCTTCGCGCCCCTCGCGCTCTATGCTC
CCGGGCAAGAAGGAGTCCCC

Downstream 100 bases:

>100_bases
GGCCGCGCGTCCGTCTGTGTTCCGAGATCAGAGGCTGAACTCGGGCCCGTCATAGGCCGTCGGCACCACGATCCGGCCGG
CGGCGATGTCCGCCGTATAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIATWNVNSVRLRTEHAVAWLKEAQPDVVCLQEIKCQTEQFPREPFEALGYNVAVHGQKGFNGVAILSKRPLEDVTNGL
AGDDSDVQARFIEAVVSVPGGVVRVCCIYLPNGNPPQTEKYDYKLGFMARLKAFTESRLKLEEPLILAGDFNVIPDARDA
ADPAAWVEDALYLPRTRAAFREIINLGMTDALRATSDAERLFTFWDYQAGAWQRDFGIRIDHLLLSPQAADRLIGAGIDK
HLRALERPSDHVPAWIDLKMAA

Sequences:

>Translated_262_residues
MRIATWNVNSVRLRTEHAVAWLKEAQPDVVCLQEIKCQTEQFPREPFEALGYNVAVHGQKGFNGVAILSKRPLEDVTNGL
AGDDSDVQARFIEAVVSVPGGVVRVCCIYLPNGNPPQTEKYDYKLGFMARLKAFTESRLKLEEPLILAGDFNVIPDARDA
ADPAAWVEDALYLPRTRAAFREIINLGMTDALRATSDAERLFTFWDYQAGAWQRDFGIRIDHLLLSPQAADRLIGAGIDK
HLRALERPSDHVPAWIDLKMAA
>Mature_262_residues
MRIATWNVNSVRLRTEHAVAWLKEAQPDVVCLQEIKCQTEQFPREPFEALGYNVAVHGQKGFNGVAILSKRPLEDVTNGL
AGDDSDVQARFIEAVVSVPGGVVRVCCIYLPNGNPPQTEKYDYKLGFMARLKAFTESRLKLEEPLILAGDFNVIPDARDA
ADPAAWVEDALYLPRTRAAFREIINLGMTDALRATSDAERLFTFWDYQAGAWQRDFGIRIDHLLLSPQAADRLIGAGIDK
HLRALERPSDHVPAWIDLKMAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=28.0898876404494, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=28.0898876404494, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=28.0898876404494, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=33.7078651685393, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=27.2030651340996, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221330655, Length=268, Percent_Identity=27.9850746268657, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17136678, Length=268, Percent_Identity=27.9850746268657, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29271; Mature: 29271

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSVRLRTEHAVAWLKEAQPDVVCLQEIKCQTEQFPREPFEALGYNVAVHGQK
CEEEEECCCEEEEEHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHCCCCEEEECCC
GFNGVAILSKRPLEDVTNGLAGDDSDVQARFIEAVVSVPGGVVRVCCIYLPNGNPPQTEK
CCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCC
YDYKLGFMARLKAFTESRLKLEEPLILAGDFNVIPDARDAADPAAWVEDALYLPRTRAAF
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
REIINLGMTDALRATSDAERLFTFWDYQAGAWQRDFGIRIDHLLLSPQAADRLIGAGIDK
HHHHHCCCHHHHHHCCCHHHHHHEECCCCCCEECCCCCEEEHEEECCHHHHHHHHCCHHH
HLRALERPSDHVPAWIDLKMAA
HHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MRIATWNVNSVRLRTEHAVAWLKEAQPDVVCLQEIKCQTEQFPREPFEALGYNVAVHGQK
CEEEEECCCEEEEEHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHCCCCEEEECCC
GFNGVAILSKRPLEDVTNGLAGDDSDVQARFIEAVVSVPGGVVRVCCIYLPNGNPPQTEK
CCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCC
YDYKLGFMARLKAFTESRLKLEEPLILAGDFNVIPDARDAADPAAWVEDALYLPRTRAAF
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
REIINLGMTDALRATSDAERLFTFWDYQAGAWQRDFGIRIDHLLLSPQAADRLIGAGIDK
HHHHHCCCHHHHHHCCCHHHHHHEECCCCCCEECCCCCEEEHEEECCHHHHHHHHCCHHH
HLRALERPSDHVPAWIDLKMAA
HHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]