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The map label for this gene is algI [H]

Identifier: 158424435

GI number: 158424435

Start: 3195834

End: 3197240

Strand: Reverse

Name: algI [H]

Synonym: AZC_2811

Alternate gene names: 158424435

Gene position: 3197240-3195834 (Counterclockwise)

Preceding gene: 158424437

Following gene: 158424434

Centisome position: 59.54

GC content: 62.19

Gene sequence:

>1407_bases
ATGACCTTCGCTTCGGTTGAGTTCATTTTCTATTTCTTTCCGCTTTTCCTGATATTCTATTTCTCAAGCCGAAAGCTGAA
GATCCGTAATCTCGTTCTGGTTTTATTTTCGCTTGCTTTTTATTCTGTCGGCTATGTGCCTTATCTGTTTCTGCTTCTGG
CGTCGATTTGTGTCAATTTCATCATCGCGCTGCGTATTGATGAGCGGCAGAACGCCTCCCGCAAGACGTTTCTCATCCTC
GGCGTCACGGCAAATGTGCTGCTGCTCGGCGTCTTCAAATATACGGATTTCGCAGTCGACAACCTGAACGTGCTTCTTGC
GCCGTTCCATCTGCGTCTGCCGGAGCCGGATATTGCCCTTCCGCTGGGAATTTCCTTCTACTCCTTCCACGCCATTTCTT
ACCTCGCCGACATTTATAAGGGCCGTGTACGGGCCAACCGCAGCCTCGGCCAGTTCACGCTCTACATGTCCATGTTCCCG
CAGCTAGTGGCCGGCCCCATCGTCCGCTACGCGACGGTGGCGCGGCAGCTCGGCGACCGGCGCGTCACCTGGGGGCGCTT
TTCCGCCGGCATGCGCCTCTTCGTCATCGGCCTCGCCTGGAAGGTGATGATCGCCGACGAGGTGGCGGCGCTGGCCGATG
GCGTCTTCGATTCCACCACCCATCCGACTTTCGTGGAGGCCCTGCTCGGCCTCTATGCCTATACGGTCCAGATCTATTTC
GACTTCGGCGGCTACTCCACCATGGCGGTGGGGCTCGGCGTGATGATGGGCTTCACGCTGCCGCGCAACTTCCGCCTGCC
CTATGCGGCGCGCAGCGTCACCGATTTCTGGCGGCGCTGGCACATGAGCCTGTCGTCCTGGCTGCGTGACTATGTGTACA
TCACGCTCGGCGGCAACCGGCGCGGCGCCACCCGCACCTATGTGAACCTCTGGATCGTCTTCCTGCTGTGCGGCCTCTGG
CACGGGGCAAGCTGGAACTTTGTCATCTGGGGCGCCTGGCACGGCGCCTTCCTCGTGCTGGAGCGGGCGTGGCTCGGGCG
CCACCTGCGGCGCCTGCCGCCAGGCGTGTCGCACCTTTATCTGGTGCTGGTCGTGATGCTGGGCTGGGTGTGGTTCCGCG
CCGAAACCTTCTCGGGCGCCATCGACATCTATCGCGGCCTCCTCGGGTTCAACGGCTTTTCCGACCAGTCGCTGGCGCTG
GGCTTCGGCCTTCACCCGCTCAGCGCCGCGGCACTGGTCGCCGGCGGACTGCTCGGCTTCTGGCGCTGGCGCCGGCCGGC
GCTGCTGAAGGGAGAGGGCTGGGTGGCGGTGGGGGCGGACTATGTCATGGTCGCCGCTCTCATGCTCGTCTGCGTGCTCT
GGGTGGGCGGCGGCTCCGGCACGCCGTTCCTCTACTACCGGTTCTGA

Upstream 100 bases:

>100_bases
GGCCAAGTCGTCGTTCGCAATTCCGTGATCCGTATTGCCTCTCGCGGCAAAGGATGGCATTGCGAGGCAGGCACTTCAGC
AGGCGGGCGGCACCCTTGAA

Downstream 100 bases:

>100_bases
CATGACGATGCTCAGCCATCACCGCCGCTATCTCGGCGCCGTCGTCCTGGTTATCCTCGCGCTGCTGCTCGCCGCCAATT
TCGTGCCGGATCCGCTGGGG

Product: membrane bound O-acyl transferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTFASVEFIFYFFPLFLIFYFSSRKLKIRNLVLVLFSLAFYSVGYVPYLFLLLASICVNFIIALRIDERQNASRKTFLIL
GVTANVLLLGVFKYTDFAVDNLNVLLAPFHLRLPEPDIALPLGISFYSFHAISYLADIYKGRVRANRSLGQFTLYMSMFP
QLVAGPIVRYATVARQLGDRRVTWGRFSAGMRLFVIGLAWKVMIADEVAALADGVFDSTTHPTFVEALLGLYAYTVQIYF
DFGGYSTMAVGLGVMMGFTLPRNFRLPYAARSVTDFWRRWHMSLSSWLRDYVYITLGGNRRGATRTYVNLWIVFLLCGLW
HGASWNFVIWGAWHGAFLVLERAWLGRHLRRLPPGVSHLYLVLVVMLGWVWFRAETFSGAIDIYRGLLGFNGFSDQSLAL
GFGLHPLSAAALVAGGLLGFWRWRRPALLKGEGWVAVGADYVMVAALMLVCVLWVGGGSGTPFLYYRF

Sequences:

>Translated_468_residues
MTFASVEFIFYFFPLFLIFYFSSRKLKIRNLVLVLFSLAFYSVGYVPYLFLLLASICVNFIIALRIDERQNASRKTFLIL
GVTANVLLLGVFKYTDFAVDNLNVLLAPFHLRLPEPDIALPLGISFYSFHAISYLADIYKGRVRANRSLGQFTLYMSMFP
QLVAGPIVRYATVARQLGDRRVTWGRFSAGMRLFVIGLAWKVMIADEVAALADGVFDSTTHPTFVEALLGLYAYTVQIYF
DFGGYSTMAVGLGVMMGFTLPRNFRLPYAARSVTDFWRRWHMSLSSWLRDYVYITLGGNRRGATRTYVNLWIVFLLCGLW
HGASWNFVIWGAWHGAFLVLERAWLGRHLRRLPPGVSHLYLVLVVMLGWVWFRAETFSGAIDIYRGLLGFNGFSDQSLAL
GFGLHPLSAAALVAGGLLGFWRWRRPALLKGEGWVAVGADYVMVAALMLVCVLWVGGGSGTPFLYYRF
>Mature_467_residues
TFASVEFIFYFFPLFLIFYFSSRKLKIRNLVLVLFSLAFYSVGYVPYLFLLLASICVNFIIALRIDERQNASRKTFLILG
VTANVLLLGVFKYTDFAVDNLNVLLAPFHLRLPEPDIALPLGISFYSFHAISYLADIYKGRVRANRSLGQFTLYMSMFPQ
LVAGPIVRYATVARQLGDRRVTWGRFSAGMRLFVIGLAWKVMIADEVAALADGVFDSTTHPTFVEALLGLYAYTVQIYFD
FGGYSTMAVGLGVMMGFTLPRNFRLPYAARSVTDFWRRWHMSLSSWLRDYVYITLGGNRRGATRTYVNLWIVFLLCGLWH
GASWNFVIWGAWHGAFLVLERAWLGRHLRRLPPGVSHLYLVLVVMLGWVWFRAETFSGAIDIYRGLLGFNGFSDQSLALG
FGLHPLSAAALVAGGLLGFWRWRRPALLKGEGWVAVGADYVMVAALMLVCVLWVGGGSGTPFLYYRF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 52963; Mature: 52832

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFASVEFIFYFFPLFLIFYFSSRKLKIRNLVLVLFSLAFYSVGYVPYLFLLLASICVNF
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIALRIDERQNASRKTFLILGVTANVLLLGVFKYTDFAVDNLNVLLAPFHLRLPEPDIAL
HEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHEECCCEEEEEEEEEECCCCCCEE
PLGISFYSFHAISYLADIYKGRVRANRSLGQFTLYMSMFPQLVAGPIVRYATVARQLGDR
ECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVTWGRFSAGMRLFVIGLAWKVMIADEVAALADGVFDSTTHPTFVEALLGLYAYTVQIYF
CCCCHHHHCCHHEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEE
DFGGYSTMAVGLGVMMGFTLPRNFRLPYAARSVTDFWRRWHMSLSSWLRDYVYITLGGNR
ECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCC
RGATRTYVNLWIVFLLCGLWHGASWNFVIWGAWHGAFLVLERAWLGRHLRRLPPGVSHLY
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHH
LVLVVMLGWVWFRAETFSGAIDIYRGLLGFNGFSDQSLALGFGLHPLSAAALVAGGLLGF
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
WRWRRPALLKGEGWVAVGADYVMVAALMLVCVLWVGGGSGTPFLYYRF
HHCCCCCEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure 
TFASVEFIFYFFPLFLIFYFSSRKLKIRNLVLVLFSLAFYSVGYVPYLFLLLASICVNF
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIALRIDERQNASRKTFLILGVTANVLLLGVFKYTDFAVDNLNVLLAPFHLRLPEPDIAL
HEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHEECCCEEEEEEEEEECCCCCCEE
PLGISFYSFHAISYLADIYKGRVRANRSLGQFTLYMSMFPQLVAGPIVRYATVARQLGDR
ECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVTWGRFSAGMRLFVIGLAWKVMIADEVAALADGVFDSTTHPTFVEALLGLYAYTVQIYF
CCCCHHHHCCHHEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEE
DFGGYSTMAVGLGVMMGFTLPRNFRLPYAARSVTDFWRRWHMSLSSWLRDYVYITLGGNR
ECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCC
RGATRTYVNLWIVFLLCGLWHGASWNFVIWGAWHGAFLVLERAWLGRHLRRLPPGVSHLY
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHH
LVLVVMLGWVWFRAETFSGAIDIYRGLLGFNGFSDQSLALGFGLHPLSAAALVAGGLLGF
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
WRWRRPALLKGEGWVAVGADYVMVAALMLVCVLWVGGGSGTPFLYYRF
HHCCCCCEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]