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The map label for this gene is ophA1 [H]

Identifier: 158424410

GI number: 158424410

Start: 3166822

End: 3167766

Strand: Reverse

Name: ophA1 [H]

Synonym: AZC_2786

Alternate gene names: 158424410

Gene position: 3167766-3166822 (Counterclockwise)

Preceding gene: 158424411

Following gene: 158424409

Centisome position: 58.99

GC content: 72.28

Gene sequence:

>945_bases
ATGCGGCGACACGGTGCGATCCTGACCCGCGTGAGCGCCATCGAGGATGCGGGCGAGGAGGTCAAGCGCTTCACCCTTCG
GGATCCCGAGGGGTGGGATCTTCCCCCGGCGCGGCCCGGCGGCCATGTGGACCTTTATCTCCCCGGAGGCCTCACACGCA
CGTATTCCCTGTGCGGAGACCCGGCGCAGCGGGACCGCTATGTGGTCGCGGTGAAGCGCGAGGCGGAGGGCCGGGGCGGC
TCCATCCGGCTGCACGACGCTGTGAGCGTGGGGCATGAGATGCACGTGGGCCTGCCGCGCGGCGGCGTGCCGCTCGGGCC
CGGCTTTCAGCTTTTCATCGCAGGCGGGATCGGGGTCACGCCCTTCCTCTCGGCGGCCGCGGCGCTGTTGGCACAGGGGC
GGGAGGACTTCCACCTGCACGTGGCGAGCCGGGGTGCCCCGCCGCTGGCGGACCAGCTCGCCCCGCTGCTCGAACGCGGC
CTCGCGTCGGTTCACGATACGACGCAGGCACCGCGGCGGGCTGTCTCCGACGTCATCAGGGGGGCGCCGGGAGAGGCTTC
CCTCGCCTGCTGCGGTCCGCTGTCCATGCTGGATGACGTCGCGGCGGCCACCGAGGGATGGCCGGCCGAGCGGGTGCATC
TGGAGCGCTTCGTGCCGCCGCCCCTCGTGCCCGATCCGGATGCGCGGGACTATCGGCTGGTGCTGGCGAAGCGGGGCATC
GAACTCGACGTGCCCGCCGGAGCCAACATGGCCGACGCGCTGGCGGCAGCGGGCGTGCAGGTGCCGGTCTCCTGCGGTGG
CGGCATCTGTGGCACCTGCGCCGTTGTCTATCTTGAGGGCCAGCCCCTGCACCGCGACCGCTGCCTGTCCCAGACCGAGC
GCGCGAGCCGGCTTATGCCGTGTGTGGCCCAATCGGCGGGCGAACGGCTGGTGCTGGACCTGTGA

Upstream 100 bases:

>100_bases
AAGGCGATCTTTGATCCCTTGATCAACCGCTCCGGCCTCAACTGCCGCATCCTCGAAGGCGGCACGGTGCGGATCGGTGA
TCTGGTGCGGAACATCTGAC

Downstream 100 bases:

>100_bases
TCCGAGGGGAGCGACGGAAGAGGCGATGTCGGAAAGCCACACTCGTCCACAACCAGAGGGTGAGGGTGGGGATTCTTGAC
CCAAACGGTATCGCATTTTG

Product: flavodoxin reductases

Products: NA

Alternate protein names: PDR [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRRHGAILTRVSAIEDAGEEVKRFTLRDPEGWDLPPARPGGHVDLYLPGGLTRTYSLCGDPAQRDRYVVAVKREAEGRGG
SIRLHDAVSVGHEMHVGLPRGGVPLGPGFQLFIAGGIGVTPFLSAAAALLAQGREDFHLHVASRGAPPLADQLAPLLERG
LASVHDTTQAPRRAVSDVIRGAPGEASLACCGPLSMLDDVAAATEGWPAERVHLERFVPPPLVPDPDARDYRLVLAKRGI
ELDVPAGANMADALAAAGVQVPVSCGGGICGTCAVVYLEGQPLHRDRCLSQTERASRLMPCVAQSAGERLVLDL

Sequences:

>Translated_314_residues
MRRHGAILTRVSAIEDAGEEVKRFTLRDPEGWDLPPARPGGHVDLYLPGGLTRTYSLCGDPAQRDRYVVAVKREAEGRGG
SIRLHDAVSVGHEMHVGLPRGGVPLGPGFQLFIAGGIGVTPFLSAAAALLAQGREDFHLHVASRGAPPLADQLAPLLERG
LASVHDTTQAPRRAVSDVIRGAPGEASLACCGPLSMLDDVAAATEGWPAERVHLERFVPPPLVPDPDARDYRLVLAKRGI
ELDVPAGANMADALAAAGVQVPVSCGGGICGTCAVVYLEGQPLHRDRCLSQTERASRLMPCVAQSAGERLVLDL
>Mature_314_residues
MRRHGAILTRVSAIEDAGEEVKRFTLRDPEGWDLPPARPGGHVDLYLPGGLTRTYSLCGDPAQRDRYVVAVKREAEGRGG
SIRLHDAVSVGHEMHVGLPRGGVPLGPGFQLFIAGGIGVTPFLSAAAALLAQGREDFHLHVASRGAPPLADQLAPLLERG
LASVHDTTQAPRRAVSDVIRGAPGEASLACCGPLSMLDDVAAATEGWPAERVHLERFVPPPLVPDPDARDYRLVLAKRGI
ELDVPAGANMADALAAAGVQVPVSCGGGICGTCAVVYLEGQPLHRDRCLSQTERASRLMPCVAQSAGERLVLDL

Specific function: Component of the electron transfer chain involved in pyridine nucleotide-dependent dihydroxylation of phthalate. Utilizes FMN to mediate electron transfer from the two-electron donor, NADH, to the one-electron acceptor, (2Fe-2S) [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=326, Percent_Identity=33.1288343558282, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787658, Length=299, Percent_Identity=27.4247491638796, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 33098; Mature: 33098

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHGAILTRVSAIEDAGEEVKRFTLRDPEGWDLPPARPGGHVDLYLPGGLTRTYSLCGD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCC
PAQRDRYVVAVKREAEGRGGSIRLHDAVSVGHEMHVGLPRGGVPLGPGFQLFIAGGIGVT
CCCCCCEEEEEEECCCCCCCEEEEEHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCCHH
PFLSAAAALLAQGREDFHLHVASRGAPPLADQLAPLLERGLASVHDTTQAPRRAVSDVIR
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAPGEASLACCGPLSMLDDVAAATEGWPAERVHLERFVPPPLVPDPDARDYRLVLAKRGI
CCCCCCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
ELDVPAGANMADALAAAGVQVPVSCGGGICGTCAVVYLEGQPLHRDRCLSQTERASRLMP
EEECCCCCCHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
CVAQSAGERLVLDL
HHHHCCCCEEEEEC
>Mature Secondary Structure
MRRHGAILTRVSAIEDAGEEVKRFTLRDPEGWDLPPARPGGHVDLYLPGGLTRTYSLCGD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCC
PAQRDRYVVAVKREAEGRGGSIRLHDAVSVGHEMHVGLPRGGVPLGPGFQLFIAGGIGVT
CCCCCCEEEEEEECCCCCCCEEEEEHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCCHH
PFLSAAAALLAQGREDFHLHVASRGAPPLADQLAPLLERGLASVHDTTQAPRRAVSDVIR
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAPGEASLACCGPLSMLDDVAAATEGWPAERVHLERFVPPPLVPDPDARDYRLVLAKRGI
CCCCCCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
ELDVPAGANMADALAAAGVQVPVSCGGGICGTCAVVYLEGQPLHRDRCLSQTERASRLMP
EEECCCCCCHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
CVAQSAGERLVLDL
HHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9851995; 1280857 [H]