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The map label for this gene is fabI1 [H]

Identifier: 158424393

GI number: 158424393

Start: 3149645

End: 3150502

Strand: Direct

Name: fabI1 [H]

Synonym: AZC_2769

Alternate gene names: 158424393

Gene position: 3149645-3150502 (Clockwise)

Preceding gene: 158424390

Following gene: 158424396

Centisome position: 58.66

GC content: 66.08

Gene sequence:

>858_bases
ATGGCGCCGAACCTCCATATTGCGACGCACCATACTTGCGTTTCGCAGGATGTTCCGCCCCGCCCCGAGGTCTCCATGAT
CGACGCCACGCAAATGCCGGCTCTGGAGGGCAAGCGCGCCCTCATCCTTGGCATCGCCAACGAGCATTCCATCGCGTACG
GATGCGCCCGCGTGTTCCGCCGCCTCGGCGCGGAAGTCGCCCTCACCTATCTCAACGAGAAGGCGCGCCCCTTCGTGGAG
CCACTGGCCCATGAGTTGGATGCCCGCATCTTTGCCCCCTGCGACGTGGAGCAGGAAGGCGCGCTGGATTCGGTTTTTGC
CGAAGTGCGCGAAACCTGGGGCGGCCTCGACATCGCGCTCCATTCCATCGCCTTCGCCCCGAAGGACGACCTGCACGGGC
GGCTGGTGGACAGTTCGGCGGCGGGGTTCGCGCTCGCCATGGATGTGTCCTGCCACTCCTTCATCCGCATGGCTCACCTC
GCCGAACCCTTGATGACCCAGGGCGGCACGCTTCTCGCCATGACCTATCAGGGCGCCAACAAGGTGGTGCCCAACTACAA
TCTCATGGGGCCGGTGAAAGCGGCGCTGGAGAGCGCCGTGCGCTATCTCGCCTATGAGCTGGGGCCGAAGGGCATCCGCG
TGCATGCCATCTCGCCCGGCCCCCTGAAGACGCGCGCCGCGTCGGGCCTCAAGGATTTCGACCTCCTGCTGAACGAGGCC
GCCACCCGCGCGCCGGTGCACGAGCTGGTGGACATCGACGACGTGGGCATGACCGCCGCCTTCCTTGCCACCCCCTTCGC
CCACCGCCTCACGGGAATGACGGTCTATGTGGACGGCGGTCTCAGCATCATGGCCTGA

Upstream 100 bases:

>100_bases
TCATCACGACGAGCCGCACGCTTTCACTTCCGCTGCTGCCAGCCGACGAAATAATAATACGGGTGCGACCAGTCGTGGGA
TTGATTCACTTCAAGGATGA

Downstream 100 bases:

>100_bases
GGCTCAGCGGGGCGGGTGGTCACCGACCAGTCCCAGCGTTCGTGGATCAGGCTCGCCGTCGAAGAAGACAGCGAGCGCCT
GACGGAAGATGCTGTCCGGA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAPNLHIATHHTCVSQDVPPRPEVSMIDATQMPALEGKRALILGIANEHSIAYGCARVFRRLGAEVALTYLNEKARPFVE
PLAHELDARIFAPCDVEQEGALDSVFAEVRETWGGLDIALHSIAFAPKDDLHGRLVDSSAAGFALAMDVSCHSFIRMAHL
AEPLMTQGGTLLAMTYQGANKVVPNYNLMGPVKAALESAVRYLAYELGPKGIRVHAISPGPLKTRAASGLKDFDLLLNEA
ATRAPVHELVDIDDVGMTAAFLATPFAHRLTGMTVYVDGGLSIMA

Sequences:

>Translated_285_residues
MAPNLHIATHHTCVSQDVPPRPEVSMIDATQMPALEGKRALILGIANEHSIAYGCARVFRRLGAEVALTYLNEKARPFVE
PLAHELDARIFAPCDVEQEGALDSVFAEVRETWGGLDIALHSIAFAPKDDLHGRLVDSSAAGFALAMDVSCHSFIRMAHL
AEPLMTQGGTLLAMTYQGANKVVPNYNLMGPVKAALESAVRYLAYELGPKGIRVHAISPGPLKTRAASGLKDFDLLLNEA
ATRAPVHELVDIDDVGMTAAFLATPFAHRLTGMTVYVDGGLSIMA
>Mature_284_residues
APNLHIATHHTCVSQDVPPRPEVSMIDATQMPALEGKRALILGIANEHSIAYGCARVFRRLGAEVALTYLNEKARPFVEP
LAHELDARIFAPCDVEQEGALDSVFAEVRETWGGLDIALHSIAFAPKDDLHGRLVDSSAAGFALAMDVSCHSFIRMAHLA
EPLMTQGGTLLAMTYQGANKVVPNYNLMGPVKAALESAVRYLAYELGPKGIRVHAISPGPLKTRAASGLKDFDLLLNEAA
TRAPVHELVDIDDVGMTAAFLATPFAHRLTGMTVYVDGGLSIMA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=255, Percent_Identity=44.7058823529412, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI87082100, Length=265, Percent_Identity=29.0566037735849, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=28.4, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 30591; Mature: 30459

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPNLHIATHHTCVSQDVPPRPEVSMIDATQMPALEGKRALILGIANEHSIAYGCARVFR
CCCCCEEEECCHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
RLGAEVALTYLNEKARPFVEPLAHELDARIFAPCDVEQEGALDSVFAEVRETWGGLDIAL
HHCHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHH
HSIAFAPKDDLHGRLVDSSAAGFALAMDVSCHSFIRMAHLAEPLMTQGGTLLAMTYQGAN
HHHCCCCCCCCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
KVVPNYNLMGPVKAALESAVRYLAYELGPKGIRVHAISPGPLKTRAASGLKDFDLLLNEA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHH
ATRAPVHELVDIDDVGMTAAFLATPFAHRLTGMTVYVDGGLSIMA
HHHCCHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCEEECC
>Mature Secondary Structure 
APNLHIATHHTCVSQDVPPRPEVSMIDATQMPALEGKRALILGIANEHSIAYGCARVFR
CCCCEEEECCHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
RLGAEVALTYLNEKARPFVEPLAHELDARIFAPCDVEQEGALDSVFAEVRETWGGLDIAL
HHCHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHH
HSIAFAPKDDLHGRLVDSSAAGFALAMDVSCHSFIRMAHLAEPLMTQGGTLLAMTYQGAN
HHHCCCCCCCCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
KVVPNYNLMGPVKAALESAVRYLAYELGPKGIRVHAISPGPLKTRAASGLKDFDLLLNEA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHH
ATRAPVHELVDIDDVGMTAAFLATPFAHRLTGMTVYVDGGLSIMA
HHHCCHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]