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The map label for this gene is cspA [C]

Identifier: 158424300

GI number: 158424300

Start: 3035748

End: 3036101

Strand: Direct

Name: cspA [C]

Synonym: AZC_2676

Alternate gene names: 158424300

Gene position: 3035748-3036101 (Clockwise)

Preceding gene: 158424299

Following gene: 158424301

Centisome position: 56.53

GC content: 61.02

Gene sequence:

>354_bases
GTGTCGATAGACGGTCAGACGACTTGGCTTTGCGCCCCCACATGTGGCGCGCCGCTGACGATATCCCTCCGAAAATCATC
CTTCGACGGCCACGCAACTTTGCGTGGCGTGACGTTCTATTCTTTCTGGAAAGGGTTTCCCATGACTACGGGAACCGTCA
AATGGTTCAACCCCGAGAAGGGCTTCGGCTTCATCGCTCCCGATGACGGCGGCGCGGATGCCTTCGTCCACATCAGCGCG
GTTGAGCGCGCCGGCCTGCCGCCCCTGCGCGAAGGCCAGAAGGTCAATTATGACCTCGTGGCCGACAAGCGCAGCGGCAA
GAGCTCGGCATCGAACCTCACGCTCGTTCCCTGA

Upstream 100 bases:

>100_bases
ACGCGGATCGTTCTTCAGTCAAAACCTTTATCTACCGGGGCTTTCGGCCCGCCGACCCGATAAAATTTGACTTGTGCGGC
CTCGGCGTCCATCATGAGTC

Downstream 100 bases:

>100_bases
CGAGCGCTTCGGATCACACCAGGCTCCGGCCGATCGCGGATGCGCGGACGGCCAGAGCGCGGTGAACCGGACCCAAGAGC
CCCGCCCCCGCCGTGGAGGC

Product: cold shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 116

Protein sequence:

>117_residues
MSIDGQTTWLCAPTCGAPLTISLRKSSFDGHATLRGVTFYSFWKGFPMTTGTVKWFNPEKGFGFIAPDDGGADAFVHISA
VERAGLPPLREGQKVNYDLVADKRSGKSSASNLTLVP

Sequences:

>Translated_117_residues
MSIDGQTTWLCAPTCGAPLTISLRKSSFDGHATLRGVTFYSFWKGFPMTTGTVKWFNPEKGFGFIAPDDGGADAFVHISA
VERAGLPPLREGQKVNYDLVADKRSGKSSASNLTLVP
>Mature_116_residues
SIDGQTTWLCAPTCGAPLTISLRKSSFDGHATLRGVTFYSFWKGFPMTTGTVKWFNPEKGFGFIAPDDGGADAFVHISAV
ERAGLPPLREGQKVNYDLVADKRSGKSSASNLTLVP

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=50, Percent_Identity=60, Blast_Score=70, Evalue=5e-14,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=48.4848484848485, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=47.6923076923077, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1787834, Length=50, Percent_Identity=56, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=56, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=48.4848484848485, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1787107, Length=52, Percent_Identity=51.9230769230769, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 12569; Mature: 12438

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDGQTTWLCAPTCGAPLTISLRKSSFDGHATLRGVTFYSFWKGFPMTTGTVKWFNPEK
CCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCCEEEEECCCC
GFGFIAPDDGGADAFVHISAVERAGLPPLREGQKVNYDLVADKRSGKSSASNLTLVP
CCEEECCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECC
>Mature Secondary Structure 
SIDGQTTWLCAPTCGAPLTISLRKSSFDGHATLRGVTFYSFWKGFPMTTGTVKWFNPEK
CCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCCEEEEECCCC
GFGFIAPDDGGADAFVHISAVERAGLPPLREGQKVNYDLVADKRSGKSSASNLTLVP
CCEEECCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]