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The map label for this gene is gatA [H]

Identifier: 158424258

GI number: 158424258

Start: 2971011

End: 2972321

Strand: Direct

Name: gatA [H]

Synonym: AZC_2634

Alternate gene names: 158424258

Gene position: 2971011-2972321 (Clockwise)

Preceding gene: 158424257

Following gene: 158424259

Centisome position: 55.33

GC content: 67.81

Gene sequence:

>1311_bases
ATGGAACCCTGTTTCCTGACCGCCGCCGAGGCGGCCTCTGCCATACGGGCCGGCACACTCACCAGCGAAGCGCTGCTCCG
CTCCTGCATCGCCCGCATCGAGGCGCGCGACCCGGTCGTGAAGGCCTGGCTCTATCTGGATAAAGCCAAGGCGATCGCCA
CCGCCCGGGAACTGGACAAGCGCCCGCCCATGGGGCCTCTGCACGGCCTGCCCATCGGCGTGAAGGACATGATCGACACC
GCCGACATGCCCACCACCTACAACTCCCCGGTGTTCCAGGACTATCGTCCGGCCCGCGACGCCGCCTGCGTGGCGGTGGC
GCGCTATTCCGGCGCCGTGATCCTCGGCAAGACAGACACGGTGGAATTCGCCGCGGCCGGCCGCCGCGCCGCCACCCGCA
ACCCGAAGAACCCCGCCCACACGCCGGGCGGCTCGTCCTCCGGCTCGGGCGCCGCGGTGGGCGACCGGCAGGTGCAGCTG
GCCTTCGGCACCCAGACCGGCGGCTCGCACATCCGCCCGGCATCCTTCAATGGCATCTATGGGATGAAGCCAACCTGGGG
CACCGTCAGCCGGGAGGGCGCCAAGCAGTATGCCAACATGCTGGACACCATCGGCTGGTATGGCCGCTCGGTCGCAGATC
TGGCCCTTGTGGGCTCCGCCTTCCGCCTGCACGACGTGGGCGAGATCGGCATTCCCAGCGTCGCCGGGCTGAGGGTTGCC
ATCTGCCGGACGCCGTTCTGGGATCAGATCGAACCGGCCGGCGCCGCAGCTTTGGAGATCGCCAGCAAACGGCTTGAGAA
GGCCGGAGCGAAGCTCATCGATCTCACCCTCCCGCCCGCCTTCGACGGCATGTTCGAAGCCCAGAACACCATCATGATGG
CGGAGGGGCGCGCCTCGTTCCTGGACGTCGCGTTGGAGTTCGGCGAACGGCTGCACGTGGACTTTCATGACCGGGTGAAC
AATAAACTCGAGATAACGCCCGAGAACCTGCTCGCGGCCTACAATCTGGCCGCGGGATGCCGCAGGGCCTTCGAGGCGCT
CTATGCCGATTTCGACGTGGTGCTCACCCCCGCCGCCCCGGGAGAGGCGCCGGAAGGCACGTACAACACCGGCAATCACA
TCTTTAACGCGATGTGGACGCTCCTTCACGTGCCCTGCCTCGGGATCCCCTGCACCACGGGCCCCACGGGCCTGCCGGTG
GGCGTGCAGATCGTCGCCCCACGCTATGGTGACGCCCGGCTGCTGGCGCTGGGAGAAGCGATGGCGCCCATCATCGATGA
GGAAACCGCGCTCTATGCGGTGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCAGTCTCTTCAGCCGGTGCTGGACGATTTCGCCGACACCCGCGCCACCCTGCTCGCCGCCGCCTGAACATTCGCGC
ACATCGGAAAGGGAATACCC

Downstream 100 bases:

>100_bases
TCCATCCGACGTACCACTATCCCGCAGCCGCCCCCGTGAACGGCTATTGCCTCGCCGCCACCCTCGCGCTGGCGGCGGAT
TATGGAGACGCATGTTCATC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MEPCFLTAAEAASAIRAGTLTSEALLRSCIARIEARDPVVKAWLYLDKAKAIATARELDKRPPMGPLHGLPIGVKDMIDT
ADMPTTYNSPVFQDYRPARDAACVAVARYSGAVILGKTDTVEFAAAGRRAATRNPKNPAHTPGGSSSGSGAAVGDRQVQL
AFGTQTGGSHIRPASFNGIYGMKPTWGTVSREGAKQYANMLDTIGWYGRSVADLALVGSAFRLHDVGEIGIPSVAGLRVA
ICRTPFWDQIEPAGAAALEIASKRLEKAGAKLIDLTLPPAFDGMFEAQNTIMMAEGRASFLDVALEFGERLHVDFHDRVN
NKLEITPENLLAAYNLAAGCRRAFEALYADFDVVLTPAAPGEAPEGTYNTGNHIFNAMWTLLHVPCLGIPCTTGPTGLPV
GVQIVAPRYGDARLLALGEAMAPIIDEETALYAVAA

Sequences:

>Translated_436_residues
MEPCFLTAAEAASAIRAGTLTSEALLRSCIARIEARDPVVKAWLYLDKAKAIATARELDKRPPMGPLHGLPIGVKDMIDT
ADMPTTYNSPVFQDYRPARDAACVAVARYSGAVILGKTDTVEFAAAGRRAATRNPKNPAHTPGGSSSGSGAAVGDRQVQL
AFGTQTGGSHIRPASFNGIYGMKPTWGTVSREGAKQYANMLDTIGWYGRSVADLALVGSAFRLHDVGEIGIPSVAGLRVA
ICRTPFWDQIEPAGAAALEIASKRLEKAGAKLIDLTLPPAFDGMFEAQNTIMMAEGRASFLDVALEFGERLHVDFHDRVN
NKLEITPENLLAAYNLAAGCRRAFEALYADFDVVLTPAAPGEAPEGTYNTGNHIFNAMWTLLHVPCLGIPCTTGPTGLPV
GVQIVAPRYGDARLLALGEAMAPIIDEETALYAVAA
>Mature_436_residues
MEPCFLTAAEAASAIRAGTLTSEALLRSCIARIEARDPVVKAWLYLDKAKAIATARELDKRPPMGPLHGLPIGVKDMIDT
ADMPTTYNSPVFQDYRPARDAACVAVARYSGAVILGKTDTVEFAAAGRRAATRNPKNPAHTPGGSSSGSGAAVGDRQVQL
AFGTQTGGSHIRPASFNGIYGMKPTWGTVSREGAKQYANMLDTIGWYGRSVADLALVGSAFRLHDVGEIGIPSVAGLRVA
ICRTPFWDQIEPAGAAALEIASKRLEKAGAKLIDLTLPPAFDGMFEAQNTIMMAEGRASFLDVALEFGERLHVDFHDRVN
NKLEITPENLLAAYNLAAGCRRAFEALYADFDVVLTPAAPGEAPEGTYNTGNHIFNAMWTLLHVPCLGIPCTTGPTGLPV
GVQIVAPRYGDARLLALGEAMAPIIDEETALYAVAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=488, Percent_Identity=25.4098360655738, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI195972892, Length=205, Percent_Identity=26.3414634146341, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=415, Percent_Identity=26.2650602409639, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=483, Percent_Identity=24.6376811594203, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=432, Percent_Identity=26.8518518518519, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=433, Percent_Identity=24.7113163972286, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=433, Percent_Identity=28.175519630485, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45550774, Length=467, Percent_Identity=25.0535331905782, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=469, Percent_Identity=24.9466950959488, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648437, Length=469, Percent_Identity=24.9466950959488, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356731, Length=204, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 46251; Mature: 46251

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPCFLTAAEAASAIRAGTLTSEALLRSCIARIEARDPVVKAWLYLDKAKAIATARELDK
CCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
RPPMGPLHGLPIGVKDMIDTADMPTTYNSPVFQDYRPARDAACVAVARYSGAVILGKTDT
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCEEEECCCCC
VEFAAAGRRAATRNPKNPAHTPGGSSSGSGAAVGDRQVQLAFGTQTGGSHIRPASFNGIY
CHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GMKPTWGTVSREGAKQYANMLDTIGWYGRSVADLALVGSAFRLHDVGEIGIPSVAGLRVA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHEECCCCCCCCCCCCCCEEE
ICRTPFWDQIEPAGAAALEIASKRLEKAGAKLIDLTLPPAFDGMFEAQNTIMMAEGRASF
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCEEEEECCCHHH
LDVALEFGERLHVDFHDRVNNKLEITPENLLAAYNLAAGCRRAFEALYADFDVVLTPAAP
HHHHHHHCCEEEEEHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GEAPEGTYNTGNHIFNAMWTLLHVPCLGIPCTTGPTGLPVGVQIVAPRYGDARLLALGEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHH
MAPIIDEETALYAVAA
HHHHHCCCCEEEEEEC
>Mature Secondary Structure
MEPCFLTAAEAASAIRAGTLTSEALLRSCIARIEARDPVVKAWLYLDKAKAIATARELDK
CCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
RPPMGPLHGLPIGVKDMIDTADMPTTYNSPVFQDYRPARDAACVAVARYSGAVILGKTDT
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCEEEECCCCC
VEFAAAGRRAATRNPKNPAHTPGGSSSGSGAAVGDRQVQLAFGTQTGGSHIRPASFNGIY
CHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GMKPTWGTVSREGAKQYANMLDTIGWYGRSVADLALVGSAFRLHDVGEIGIPSVAGLRVA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHEECCCCCCCCCCCCCCEEE
ICRTPFWDQIEPAGAAALEIASKRLEKAGAKLIDLTLPPAFDGMFEAQNTIMMAEGRASF
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCEEEEECCCHHH
LDVALEFGERLHVDFHDRVNNKLEITPENLLAAYNLAAGCRRAFEALYADFDVVLTPAAP
HHHHHHHCCEEEEEHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GEAPEGTYNTGNHIFNAMWTLLHVPCLGIPCTTGPTGLPVGVQIVAPRYGDARLLALGEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHH
MAPIIDEETALYAVAA
HHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA