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The map label for this gene is lacI [H]

Identifier: 158424235

GI number: 158424235

Start: 2946207

End: 2947280

Strand: Reverse

Name: lacI [H]

Synonym: AZC_2611

Alternate gene names: 158424235

Gene position: 2947280-2946207 (Counterclockwise)

Preceding gene: 158424236

Following gene: 158424234

Centisome position: 54.89

GC content: 68.72

Gene sequence:

>1074_bases
ATGCCGCCGCTCACGCGCAAGCCCACCATGCGGGACGTCTCGCGTCTCGCGGGCGTCTCCACGATGACCGTCTCGCGGGT
CCTGGCCGACCCCGAGACGGTGTCGCGGGAGACGCGCTCGCGCGTTCTGGCGGCGGTGGAGCAACTGGGCTATGTGCCCG
ACCGGGTGGCGGGAAGCCTGTCTTCCCGCCGCACCAACGTGGTGGCGGCGATCCTGCCGACCCTGACCAACGTCAATTTC
GCCGACACCGCGCACGCTCTGACGGAGGTGCTGCGACCGGCCGATTATCAGCTGCTCATCGGCTACACAATGTACCGGCT
GGACGAGGAGGAGCGCCTGTTAAGGGCCATGCTCCCCCGCCGACCGGACGCGGTGGTGGTGGCCAGTTCCGTGCACACGA
AGACCGCTTCCCAGATGCTGCTGCGGGCAGGCGTGCCGGTGGTGGAGATCTGGGACAATCCCGGTCAGCCCATCGATCAT
GCGGTGGGATTTTCCAATTATGAGGTGGGCCGGGCGGCGGCGCATCATCTCATCTCCCTCGGCCATCGTCGCATCGGCGC
CCTCGGCCCCGGCAGGGACGGCGAGGCGCGGGATTTTCGCGGTGAGGACCGCATGAAGGGCTTTGCCGCCGCTCTGCGCG
AGGCGGGCCTGTCGGACGAATGGGTGCTGCATTTCGGTGATGTGCCGGTCGCCTTCGATCACGGCCGCCAGTCGTTCGCC
GCCATGCTGGAGCGGGCGCCGGATGTGGAGGCGCTGTTTGCCGTCTCCGACATTTCCGCCTGGGGCGCGCTGATGGAGTG
CCAGCGGCGCGGCATCCGCGTGCCCGACGACCTGTCGCTGCTCGGCTTCGGCGATTTCGACTTCGGCCGCCAGTGCGTGC
CGATGCTGAGCACCATGGGCATCGACGCGCGGGCCATCGGCACGGCAGCGGGCGAACTGCTGATGCGGCTTCTTCATGAC
GACAGCGGCGCGGCGGCGGCGCCCGAGCGCATCGATGTGGGCTTCCGGCTGATCGAGCGGGAGACCACCGCGCCGTCCCG
CAAGCGCCGCAAGAATGGGAGGAAGTCCAGTTGA

Upstream 100 bases:

>100_bases
CGCACGCAGGCCCGCGCGGTGACCGAGGACATCGTGAAGATGTTCACCGGCCGCTTCTATCACATCGTCTACAAGCGTCA
GGCGGGGTCCTGAGGCGATC

Downstream 100 bases:

>100_bases
GAGAGACCATCGGCATCATCGGCGTCGGCGTCATGGGCAGCGCCATCGCGACGCGCTTCCTGGAGCGCGGCCGCACCGTG
CATCTGCGCGACATGGACCC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPPLTRKPTMRDVSRLAGVSTMTVSRVLADPETVSRETRSRVLAAVEQLGYVPDRVAGSLSSRRTNVVAAILPTLTNVNF
ADTAHALTEVLRPADYQLLIGYTMYRLDEEERLLRAMLPRRPDAVVVASSVHTKTASQMLLRAGVPVVEIWDNPGQPIDH
AVGFSNYEVGRAAAHHLISLGHRRIGALGPGRDGEARDFRGEDRMKGFAAALREAGLSDEWVLHFGDVPVAFDHGRQSFA
AMLERAPDVEALFAVSDISAWGALMECQRRGIRVPDDLSLLGFGDFDFGRQCVPMLSTMGIDARAIGTAAGELLMRLLHD
DSGAAAAPERIDVGFRLIERETTAPSRKRRKNGRKSS

Sequences:

>Translated_357_residues
MPPLTRKPTMRDVSRLAGVSTMTVSRVLADPETVSRETRSRVLAAVEQLGYVPDRVAGSLSSRRTNVVAAILPTLTNVNF
ADTAHALTEVLRPADYQLLIGYTMYRLDEEERLLRAMLPRRPDAVVVASSVHTKTASQMLLRAGVPVVEIWDNPGQPIDH
AVGFSNYEVGRAAAHHLISLGHRRIGALGPGRDGEARDFRGEDRMKGFAAALREAGLSDEWVLHFGDVPVAFDHGRQSFA
AMLERAPDVEALFAVSDISAWGALMECQRRGIRVPDDLSLLGFGDFDFGRQCVPMLSTMGIDARAIGTAAGELLMRLLHD
DSGAAAAPERIDVGFRLIERETTAPSRKRRKNGRKSS
>Mature_356_residues
PPLTRKPTMRDVSRLAGVSTMTVSRVLADPETVSRETRSRVLAAVEQLGYVPDRVAGSLSSRRTNVVAAILPTLTNVNFA
DTAHALTEVLRPADYQLLIGYTMYRLDEEERLLRAMLPRRPDAVVVASSVHTKTASQMLLRAGVPVVEIWDNPGQPIDHA
VGFSNYEVGRAAAHHLISLGHRRIGALGPGRDGEARDFRGEDRMKGFAAALREAGLSDEWVLHFGDVPVAFDHGRQSFAA
MLERAPDVEALFAVSDISAWGALMECQRRGIRVPDDLSLLGFGDFDFGRQCVPMLSTMGIDARAIGTAAGELLMRLLHDD
SGAAAAPERIDVGFRLIERETTAPSRKRRKNGRKSS

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=333, Percent_Identity=33.033033033033, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1790715, Length=337, Percent_Identity=28.7833827893175, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787948, Length=350, Percent_Identity=30.2857142857143, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790369, Length=308, Percent_Identity=28.2467532467532, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789068, Length=354, Percent_Identity=28.5310734463277, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788474, Length=323, Percent_Identity=28.4829721362229, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=29.1946308724832, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=24.7787610619469, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=26.1904761904762, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787906, Length=273, Percent_Identity=26.7399267399267, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=28.391167192429, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786540, Length=361, Percent_Identity=25.4847645429363, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39016; Mature: 38885

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLTRKPTMRDVSRLAGVSTMTVSRVLADPETVSRETRSRVLAAVEQLGYVPDRVAGSL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SSRRTNVVAAILPTLTNVNFADTAHALTEVLRPADYQLLIGYTMYRLDEEERLLRAMLPR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHCCHHHHHHHHHCCC
RPDAVVVASSVHTKTASQMLLRAGVPVVEIWDNPGQPIDHAVGFSNYEVGRAAAHHLISL
CCCEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHH
GHRRIGALGPGRDGEARDFRGEDRMKGFAAALREAGLSDEWVLHFGDVPVAFDHGRQSFA
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHCCHHHHH
AMLERAPDVEALFAVSDISAWGALMECQRRGIRVPDDLSLLGFGDFDFGRQCVPMLSTMG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCC
IDARAIGTAAGELLMRLLHDDSGAAAAPERIDVGFRLIERETTAPSRKRRKNGRKSS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
PPLTRKPTMRDVSRLAGVSTMTVSRVLADPETVSRETRSRVLAAVEQLGYVPDRVAGSL
CCCCCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SSRRTNVVAAILPTLTNVNFADTAHALTEVLRPADYQLLIGYTMYRLDEEERLLRAMLPR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHCCHHHHHHHHHCCC
RPDAVVVASSVHTKTASQMLLRAGVPVVEIWDNPGQPIDHAVGFSNYEVGRAAAHHLISL
CCCEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHH
GHRRIGALGPGRDGEARDFRGEDRMKGFAAALREAGLSDEWVLHFGDVPVAFDHGRQSFA
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHCCHHHHH
AMLERAPDVEALFAVSDISAWGALMECQRRGIRVPDDLSLLGFGDFDFGRQCVPMLSTMG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCC
IDARAIGTAAGELLMRLLHDDSGAAAAPERIDVGFRLIERETTAPSRKRRKNGRKSS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]