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The map label for this gene is ppaX [H]

Identifier: 158424144

GI number: 158424144

Start: 2857409

End: 2858101

Strand: Reverse

Name: ppaX [H]

Synonym: AZC_2520

Alternate gene names: 158424144

Gene position: 2858101-2857409 (Counterclockwise)

Preceding gene: 158424145

Following gene: 158424143

Centisome position: 53.23

GC content: 69.7

Gene sequence:

>693_bases
ATGACCCTTGAGCCCATCCTCGCCCTGTTCGACTGCGACGGCACGCTGGTGGACAGTCAGCACATGATCGTCGCCGCCAT
GGGCACGGCCTATGCCCGGCAGGGCCTCCCCGCGCCGGACCGGCTGCGCATGCTCTCCATCGTCGGCCTGTCGCTGCGCG
AAGCCTTCACCGACCTCGCCGAGGGCGATGCCGCCTTTCCCGTGGAGGAGATGGTGGAAGCCTATCGCAGCGCCTTCATG
GAGCTGCGCGACCGCGAGCCGCCCGAGCCCATGTACCCCTTCGCCCGCGATACGCTCGATGGGCTGCGCCAGCGCGACGA
CATGCTGCTCGGCATGGTGACGGGCAAGGCCCGGCGTGGCGTCACGCGCGTTCTGGCGGCCAATGCCATGGACGGCTGGT
TCTCGACCATCCAGACCGCCGACGATGCGCCCTCCAAGCCCCATCCGGCCATGGTGCAGCAGGCCATGGCGGAGATGGGA
CAGCCCTCCGCCCGCACGGTGGTGATCGGCGACACCGGCTTCGACATGGCCATGGCCAAGGCGGCCGGGGCCCATGCCAT
CGGTGTCGCCTGGGGCTATCATCCGCTGGAACAACTCACTGCGGCCGGTGCCGATGTGATCGTGCGCGACTTCCGCGAGA
TTCCGGACGCCATTGCGGCGCTGGTCGGCGGTCGCGCGCCCGCCGTCGCCTGA

Upstream 100 bases:

>100_bases
GCCAGTGCAAGGCGCGCGGCTTCAAGCAGGTGGTGTTCATCGATGCCAACGGCACCACCACCTGGACCCTCGACCTCGAC
ACCGACAAGTTGACCCAGAA

Downstream 100 bases:

>100_bases
GATTTTCGAGGAACCGAGCCATGCGCGACATCCTGAGCGACCTTGAGCAGTCGCCGCTCGATCCGGCCGAGGCCATCCGC
GCGCAGGGCCGCCGCACGCT

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTLEPILALFDCDGTLVDSQHMIVAAMGTAYARQGLPAPDRLRMLSIVGLSLREAFTDLAEGDAAFPVEEMVEAYRSAFM
ELRDREPPEPMYPFARDTLDGLRQRDDMLLGMVTGKARRGVTRVLAANAMDGWFSTIQTADDAPSKPHPAMVQQAMAEMG
QPSARTVVIGDTGFDMAMAKAAGAHAIGVAWGYHPLEQLTAAGADVIVRDFREIPDAIAALVGGRAPAVA

Sequences:

>Translated_230_residues
MTLEPILALFDCDGTLVDSQHMIVAAMGTAYARQGLPAPDRLRMLSIVGLSLREAFTDLAEGDAAFPVEEMVEAYRSAFM
ELRDREPPEPMYPFARDTLDGLRQRDDMLLGMVTGKARRGVTRVLAANAMDGWFSTIQTADDAPSKPHPAMVQQAMAEMG
QPSARTVVIGDTGFDMAMAKAAGAHAIGVAWGYHPLEQLTAAGADVIVRDFREIPDAIAALVGGRAPAVA
>Mature_229_residues
TLEPILALFDCDGTLVDSQHMIVAAMGTAYARQGLPAPDRLRMLSIVGLSLREAFTDLAEGDAAFPVEEMVEAYRSAFME
LRDREPPEPMYPFARDTLDGLRQRDDMLLGMVTGKARRGVTRVLAANAMDGWFSTIQTADDAPSKPHPAMVQQAMAEMGQ
PSARTVVIGDTGFDMAMAKAAGAHAIGVAWGYHPLEQLTAAGADVIVRDFREIPDAIAALVGGRAPAVA

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=29.7413793103448, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 24646; Mature: 24514

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEPILALFDCDGTLVDSQHMIVAAMGTAYARQGLPAPDRLRMLSIVGLSLREAFTDLA
CCHHHHHHEECCCCCEECCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EGDAAFPVEEMVEAYRSAFMELRDREPPEPMYPFARDTLDGLRQRDDMLLGMVTGKARRG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
VTRVLAANAMDGWFSTIQTADDAPSKPHPAMVQQAMAEMGQPSARTVVIGDTGFDMAMAK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
AAGAHAIGVAWGYHPLEQLTAAGADVIVRDFREIPDAIAALVGGRAPAVA
HCCCCEEEHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLEPILALFDCDGTLVDSQHMIVAAMGTAYARQGLPAPDRLRMLSIVGLSLREAFTDLA
CHHHHHHEECCCCCEECCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EGDAAFPVEEMVEAYRSAFMELRDREPPEPMYPFARDTLDGLRQRDDMLLGMVTGKARRG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
VTRVLAANAMDGWFSTIQTADDAPSKPHPAMVQQAMAEMGQPSARTVVIGDTGFDMAMAK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
AAGAHAIGVAWGYHPLEQLTAAGADVIVRDFREIPDAIAALVGGRAPAVA
HCCCCEEEHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]