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The map label for this gene is ycdT [H]

Identifier: 158424141

GI number: 158424141

Start: 2854924

End: 2855982

Strand: Reverse

Name: ycdT [H]

Synonym: AZC_2517

Alternate gene names: 158424141

Gene position: 2855982-2854924 (Counterclockwise)

Preceding gene: 158424142

Following gene: 158424140

Centisome position: 53.19

GC content: 64.78

Gene sequence:

>1059_bases
ATGGACATCGAATTCATCAGATTGCGGAAGCGGATGTTGCGGCTGTTGTTCGCCATCGGCTTTGTCTGCAACGGATGGTT
TCTGATTTTCGAGAGCACCAGCGGCATCATCACCGCGCGTGATGCCTATGTGACCTATCCCTCGGTCATGTTCATCTATG
CCGCCGGCTTCTTTGTCATCGAACTGGGTGATCGGTGGCGCGCAGCGACGGAGTGGCTCGCCTTCCTGTCCTTCTGTGCC
AATTGCGCCAGCGGCGTGCTGTCCTTCACGGCCGCGGACGAACCCGTGCGCTACTACGCCATTGCCGATACGCTGCAATG
GATCCCGCTCATCAGCATCATCGCCTTCATGATCTTTCCCGGCCGCCAGAGCGTGGTGGCGTCGGTGGTGGGCTTCCTCA
TTCCGGCGGGAACGGCGGGCTGGGTGTGGTGGGGAAGCGATGCAGCGACCTGGCCGGCCACCACGAGCACGCTTTTGTTC
AATGCGCTCGTCATCCATGTGGTGTCGGTGGCGGCCCTCGTTCTGTTCTCGCATTCGCAGGCCGCCTTCCAGCAGGCCCG
GGCGGAGGCCAGGATGATGGAGAGTGCGGCCCTCTCCGACCCGCTCACGGGCATTGCCAACCGGCGCGGCATCGAGCGGG
CGCTGGAGACACAGACGGCGGCTTCGGTGCCCCGCCCGTGGGCGCTCATCCTTCTGGACATGGATCACTTCAAGATGGTC
AACGACGAGCGTGGCCACGTCTTTGGGGATCGCGTGCTCCAGAATGTGGTGAATGCGCTCCGCCCTATCCTGCGTGGCGG
CGATGTCATCGGCCGGTGGGGCGGCGACGAATTTCTGGTGATCTCCCCCGGCGCGCCAGCGGCCGAGGCGGAAGCGCTGG
CCGAGCGGCTGCGGCAGGCGGTGGCCGGCCTTCCGGAGGATGACAGCGGCGGCGTGACCATCTCCGTCGGCATCGCGCTG
TGGAATGGGGAAGGCGGCCTTGTGGAGGCGCTCCGGCAGGTGGACGCGGCGCTTTATCGCTCCAAGCAGATGGGCCGGAA
CCGGGTGGTGTTTGCCTGA

Upstream 100 bases:

>100_bases
TCCTGTCGTCGCCTAGCCGTCCTGCTCCCGAGCCGAGGGCGGCCGTCTGCTTGCGTGTGTCGGTGGCCTATGTCCAAGTG
CCCGCCTGGGGAGTGCTGCG

Downstream 100 bases:

>100_bases
GGCGGAAGAGCGGGCCCGGGTGCTGCGTCGAATGCGCCATTCGCGATAACGTGCGTATCTGGATGGAGCACCGCTTCCCA
AGGCTGACAGGCTCACAACA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MDIEFIRLRKRMLRLLFAIGFVCNGWFLIFESTSGIITARDAYVTYPSVMFIYAAGFFVIELGDRWRAATEWLAFLSFCA
NCASGVLSFTAADEPVRYYAIADTLQWIPLISIIAFMIFPGRQSVVASVVGFLIPAGTAGWVWWGSDAATWPATTSTLLF
NALVIHVVSVAALVLFSHSQAAFQQARAEARMMESAALSDPLTGIANRRGIERALETQTAASVPRPWALILLDMDHFKMV
NDERGHVFGDRVLQNVVNALRPILRGGDVIGRWGGDEFLVISPGAPAAEAEALAERLRQAVAGLPEDDSGGVTISVGIAL
WNGEGGLVEALRQVDAALYRSKQMGRNRVVFA

Sequences:

>Translated_352_residues
MDIEFIRLRKRMLRLLFAIGFVCNGWFLIFESTSGIITARDAYVTYPSVMFIYAAGFFVIELGDRWRAATEWLAFLSFCA
NCASGVLSFTAADEPVRYYAIADTLQWIPLISIIAFMIFPGRQSVVASVVGFLIPAGTAGWVWWGSDAATWPATTSTLLF
NALVIHVVSVAALVLFSHSQAAFQQARAEARMMESAALSDPLTGIANRRGIERALETQTAASVPRPWALILLDMDHFKMV
NDERGHVFGDRVLQNVVNALRPILRGGDVIGRWGGDEFLVISPGAPAAEAEALAERLRQAVAGLPEDDSGGVTISVGIAL
WNGEGGLVEALRQVDAALYRSKQMGRNRVVFA
>Mature_352_residues
MDIEFIRLRKRMLRLLFAIGFVCNGWFLIFESTSGIITARDAYVTYPSVMFIYAAGFFVIELGDRWRAATEWLAFLSFCA
NCASGVLSFTAADEPVRYYAIADTLQWIPLISIIAFMIFPGRQSVVASVVGFLIPAGTAGWVWWGSDAATWPATTSTLLF
NALVIHVVSVAALVLFSHSQAAFQQARAEARMMESAALSDPLTGIANRRGIERALETQTAASVPRPWALILLDMDHFKMV
NDERGHVFGDRVLQNVVNALRPILRGGDVIGRWGGDEFLVISPGAPAAEAEALAERLRQAVAGLPEDDSGGVTISVGIAL
WNGEGGLVEALRQVDAALYRSKQMGRNRVVFA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=40.1197604790419, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=38.6363636363636, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=38.4615384615385, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=35.4651162790698, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=34.8387096774194, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=37.2670807453416, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081977, Length=197, Percent_Identity=32.48730964467, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=33.7278106508876, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=30, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787056, Length=161, Percent_Identity=33.5403726708075, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=33.1428571428571, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=186, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38418; Mature: 38418

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEFIRLRKRMLRLLFAIGFVCNGWFLIFESTSGIITARDAYVTYPSVMFIYAAGFFVI
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCEEEE
ELGDRWRAATEWLAFLSFCANCASGVLSFTAADEPVRYYAIADTLQWIPLISIIAFMIFP
EECCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
GRQSVVASVVGFLIPAGTAGWVWWGSDAATWPATTSTLLFNALVIHVVSVAALVLFSHSQ
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
AAFQQARAEARMMESAALSDPLTGIANRRGIERALETQTAASVPRPWALILLDMDHFKMV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHC
NDERGHVFGDRVLQNVVNALRPILRGGDVIGRWGGDEFLVISPGAPAAEAEALAERLRQA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
VAGLPEDDSGGVTISVGIALWNGEGGLVEALRQVDAALYRSKQMGRNRVVFA
HCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDIEFIRLRKRMLRLLFAIGFVCNGWFLIFESTSGIITARDAYVTYPSVMFIYAAGFFVI
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCEEEE
ELGDRWRAATEWLAFLSFCANCASGVLSFTAADEPVRYYAIADTLQWIPLISIIAFMIFP
EECCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
GRQSVVASVVGFLIPAGTAGWVWWGSDAATWPATTSTLLFNALVIHVVSVAALVLFSHSQ
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
AAFQQARAEARMMESAALSDPLTGIANRRGIERALETQTAASVPRPWALILLDMDHFKMV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHC
NDERGHVFGDRVLQNVVNALRPILRGGDVIGRWGGDEFLVISPGAPAAEAEALAERLRQA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
VAGLPEDDSGGVTISVGIALWNGEGGLVEALRQVDAALYRSKQMGRNRVVFA
HCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]