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The map label for this gene is potD [C]

Identifier: 158424099

GI number: 158424099

Start: 2811685

End: 2812809

Strand: Direct

Name: potD [C]

Synonym: AZC_2475

Alternate gene names: 158424099

Gene position: 2811685-2812809 (Clockwise)

Preceding gene: 158424098

Following gene: 158424100

Centisome position: 52.36

GC content: 65.33

Gene sequence:

>1125_bases
GTGTCTCAGGTCCTCATAACAAACTTCAGAGGTGAGCCCATGACTGACGTTTCCGGTACCGGTCCCTCCCGCCGCACGCT
GCTCAAGAGCGCCGCCGCCGGCGCAGCCGCGGTCATGATGCCGGCCGTCTGGTCGCCCTCCCGCGCCGCCGGCAAACGCA
TCGTAGTGCGCGACGACGGCGGCATCTACACCAAGGCCTACGGCGCGGTGTTCTATAAGCCGTTCACCGAGAAGACCGGC
ATCGAGGTGGTGGGCGTGCAGGCCGCCGCCGAACCGACCGCTCAGATCAAGAGCATGGTAGAGACGAAGAACTACACCTG
GGACATGGCCAAGATCAGCAAGCCGGCCATCCTGATCCTCACCGACGGCGGCACGCCCTATCTTGAGAAGCATGGCCTCG
AGAGCGATCCGGTGATCGCGTCGATCCCGGCCCAGTACATGTCCCCCTACGGCGTCGGCACGAACGTCTATACGACGGTG
CTCGCCTATCGCACGGACGCTTTCCAGGGCAAGAAGGCGCCCAAGTCCTGGGCCGACTTCTGGAACGTGAAGGACTTCCC
CGGCCGCCGCGCCATGCGCAAGCATCCCTTCGACACCGTCGAGGAAGCGCTGATGGCGACCGGCGTCCCCACCAACCTCG
TCTACCCCTGCAATGTGGACAAGGCCTTCGCGGCGCTCGACCAGATCAAGCCGAACATCGCCGTCTGGTGGACCTCCGGC
GCGCTGGTGGAACAGGTCCTCACCTCCGGTGAGGTGGACATGATCCCGACCTGGGTTTCCCGCGCACAGGCCGCGCAGGC
CAAGGGCGCCCCGGTGGAGATCATCTGGGACCAGGGCATCTGGGGCGGCGACCACTGGTCGATCCTCGCCGGCACGCCCA
ACGCCGACGCCTGCCGCGAGTTCATCAAGTTCGCCTCCGATCCGGAGCGCATGGCCAAGCTGACCGAATTCTTCCCCGCC
GGCGTCACCCAGCCGAAGGCGTTCGACTACATCAAGCCGGACATCGCCAAGAACTGCCCGACCTTCCCGGCCAACATTCA
GTCCGGCCTCCAGATCGACGCTGCCTACTGGCTGAAGAACCAGGCGGCCGTCATCGAGCGCTTCAACAGCTGGGTGCTCG
GCTGA

Upstream 100 bases:

>100_bases
GCGGTGAAGGCCGCCTTCGACCCCGACAACCTCTTCAACCCCGGCCGCCTGCTGCCTCCCGCAGCCTGAGCCGGGAAACG
GCCGATCCCACGTTCAAACA

Downstream 100 bases:

>100_bases
TCCCACTCGGCGCCCGCTCTCCCCTCCCCAGGGCGCCGAGCGTGCCGGCCGGATCTTCCCCATCCGGCCGGCACCCCCTT
CTTGTCGCAGACACTTGTCC

Product: extracellular substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSQVLITNFRGEPMTDVSGTGPSRRTLLKSAAAGAAAVMMPAVWSPSRAAGKRIVVRDDGGIYTKAYGAVFYKPFTEKTG
IEVVGVQAAAEPTAQIKSMVETKNYTWDMAKISKPAILILTDGGTPYLEKHGLESDPVIASIPAQYMSPYGVGTNVYTTV
LAYRTDAFQGKKAPKSWADFWNVKDFPGRRAMRKHPFDTVEEALMATGVPTNLVYPCNVDKAFAALDQIKPNIAVWWTSG
ALVEQVLTSGEVDMIPTWVSRAQAAQAKGAPVEIIWDQGIWGGDHWSILAGTPNADACREFIKFASDPERMAKLTEFFPA
GVTQPKAFDYIKPDIAKNCPTFPANIQSGLQIDAAYWLKNQAAVIERFNSWVLG

Sequences:

>Translated_374_residues
MSQVLITNFRGEPMTDVSGTGPSRRTLLKSAAAGAAAVMMPAVWSPSRAAGKRIVVRDDGGIYTKAYGAVFYKPFTEKTG
IEVVGVQAAAEPTAQIKSMVETKNYTWDMAKISKPAILILTDGGTPYLEKHGLESDPVIASIPAQYMSPYGVGTNVYTTV
LAYRTDAFQGKKAPKSWADFWNVKDFPGRRAMRKHPFDTVEEALMATGVPTNLVYPCNVDKAFAALDQIKPNIAVWWTSG
ALVEQVLTSGEVDMIPTWVSRAQAAQAKGAPVEIIWDQGIWGGDHWSILAGTPNADACREFIKFASDPERMAKLTEFFPA
GVTQPKAFDYIKPDIAKNCPTFPANIQSGLQIDAAYWLKNQAAVIERFNSWVLG
>Mature_373_residues
SQVLITNFRGEPMTDVSGTGPSRRTLLKSAAAGAAAVMMPAVWSPSRAAGKRIVVRDDGGIYTKAYGAVFYKPFTEKTGI
EVVGVQAAAEPTAQIKSMVETKNYTWDMAKISKPAILILTDGGTPYLEKHGLESDPVIASIPAQYMSPYGVGTNVYTTVL
AYRTDAFQGKKAPKSWADFWNVKDFPGRRAMRKHPFDTVEEALMATGVPTNLVYPCNVDKAFAALDQIKPNIAVWWTSGA
LVEQVLTSGEVDMIPTWVSRAQAAQAKGAPVEIIWDQGIWGGDHWSILAGTPNADACREFIKFASDPERMAKLTEFFPAG
VTQPKAFDYIKPDIAKNCPTFPANIQSGLQIDAAYWLKNQAAVIERFNSWVLG

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40779; Mature: 40648

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVLITNFRGEPMTDVSGTGPSRRTLLKSAAAGAAAVMMPAVWSPSRAAGKRIVVRDDG
CCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCHHHCCEEEEECCC
GIYTKAYGAVFYKPFTEKTGIEVVGVQAAAEPTAQIKSMVETKNYTWDMAKISKPAILIL
CEEEEECCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEHHHCCCCEEEEE
TDGGTPYLEKHGLESDPVIASIPAQYMSPYGVGTNVYTTVLAYRTDAFQGKKAPKSWADF
ECCCCCHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
WNVKDFPGRRAMRKHPFDTVEEALMATGVPTNLVYPCNVDKAFAALDQIKPNIAVWWTSG
CCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCEEEEECCC
ALVEQVLTSGEVDMIPTWVSRAQAAQAKGAPVEIIWDQGIWGGDHWSILAGTPNADACRE
HHHHHHHCCCCCEECHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHH
FIKFASDPERMAKLTEFFPAGVTQPKAFDYIKPDIAKNCPTFPANIQSGLQIDAAYWLKN
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEHHHHHHH
QAAVIERFNSWVLG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SQVLITNFRGEPMTDVSGTGPSRRTLLKSAAAGAAAVMMPAVWSPSRAAGKRIVVRDDG
CCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCHHHCCEEEEECCC
GIYTKAYGAVFYKPFTEKTGIEVVGVQAAAEPTAQIKSMVETKNYTWDMAKISKPAILIL
CEEEEECCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEHHHCCCCEEEEE
TDGGTPYLEKHGLESDPVIASIPAQYMSPYGVGTNVYTTVLAYRTDAFQGKKAPKSWADF
ECCCCCHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
WNVKDFPGRRAMRKHPFDTVEEALMATGVPTNLVYPCNVDKAFAALDQIKPNIAVWWTSG
CCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCEEEEECCC
ALVEQVLTSGEVDMIPTWVSRAQAAQAKGAPVEIIWDQGIWGGDHWSILAGTPNADACRE
HHHHHHHCCCCCEECHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHH
FIKFASDPERMAKLTEFFPAGVTQPKAFDYIKPDIAKNCPTFPANIQSGLQIDAAYWLKN
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEHHHHHHH
QAAVIERFNSWVLG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA